Screening of Genes Associated with Immune Infiltration of Discoid Lupus Erythematosus Based on Weighted Gene Co-expression Network Analysis
Biochem Genet. 2024 Mar 7. doi: 10.1007/s10528-023-10603-6. Online ahead of print.ABSTRACTDiscoid lupus erythematosus (DLE) is a disorder of the immune system commonly seen in women of childbearing age. The pathophysiology and aetiology are still poorly understood, and no cure is presently available. Therefore, there is an urgent need to explore the underlying molecular mechanisms, as well as search for new therapeutic targets. Gene expression data from skin biopsies samples of DLE patients and healthy controls were downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between DLE ...
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Yuru Han Shuang Liu Shuo Shi Yongyong Shu Changlian Lu Xuefeng Gu Source Type: research
Bioinformatic Analysis of m6A Regulator-Mediated RNA Methylation Modification Patterns and Immune Microenvironment Characterization in Endometriosis
In conclusions, our integrated bioinformatics analysis results demonstrated that m6A-related genes METTL3, CBLL1 and YTHDF2 may be useful biomarkers for endometriosis in ectopic endometrium. The potential therapeutic approach of targeting m6A regulators holds promise for the treatment of endometriosis.PMID:38451401 | DOI:10.1007/s10528-024-10725-5 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Weilin Zheng Zhiyi Fu Xi Tan Xuefang Liang Lixing Cao Source Type: research
Screening of Genes Associated with Immune Infiltration of Discoid Lupus Erythematosus Based on Weighted Gene Co-expression Network Analysis
Biochem Genet. 2024 Mar 7. doi: 10.1007/s10528-023-10603-6. Online ahead of print.ABSTRACTDiscoid lupus erythematosus (DLE) is a disorder of the immune system commonly seen in women of childbearing age. The pathophysiology and aetiology are still poorly understood, and no cure is presently available. Therefore, there is an urgent need to explore the underlying molecular mechanisms, as well as search for new therapeutic targets. Gene expression data from skin biopsies samples of DLE patients and healthy controls were downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between DLE ...
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Yuru Han Shuang Liu Shuo Shi Yongyong Shu Changlian Lu Xuefeng Gu Source Type: research
Bioinformatic Analysis of m6A Regulator-Mediated RNA Methylation Modification Patterns and Immune Microenvironment Characterization in Endometriosis
In conclusions, our integrated bioinformatics analysis results demonstrated that m6A-related genes METTL3, CBLL1 and YTHDF2 may be useful biomarkers for endometriosis in ectopic endometrium. The potential therapeutic approach of targeting m6A regulators holds promise for the treatment of endometriosis.PMID:38451401 | DOI:10.1007/s10528-024-10725-5 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Weilin Zheng Zhiyi Fu Xi Tan Xuefang Liang Lixing Cao Source Type: research
Immunogenic Cell Death-related Signature Evaluates the Tumor Microenvironment and Predicts the Prognosis in Diffuse Large B-Cell Lymphoma
In conclusion, the present study provides novel insights into the role of ICD in DLBCL by identifying a new biomarker for the disease and may have implications for the development of immune-targeted therapies for the tumor.PMID:38446321 | DOI:10.1007/s10528-024-10697-6 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 6, 2024 Category: Genetics & Stem Cells Authors: Shengqiang Huang Wenbin Liu Qiuling Zhao Ting Chen Ruyi Huang Liangliang Dong Zilin Nian Lin Yang Source Type: research
The Role of MicroRNA-206 in the Regulation of Diabetic Wound Healing via Hypoxia-Inducible Factor 1-Alpha
This study aimed to investigate the abnormal expression of miRNAs in diabetic wound healing and the potential therapeutic role of modulating the miR-206/HIF-1α pathway. MicroRNA assays were used to identify differentially expressed miRNAs in diabetic wound sites and adjacent areas. In vitro models and a rat diabetic model were established to evaluate the effects of miR-206 on HIF-1α regulation and wound healing. The study revealed differential expression of miR-206 in diabetic wound tissues, its interaction with HIF-1α, and the inhibitory effect of miR-206 on cell growth under high glucose conditions. Modulating the miR...
Source: Biochemical Genetics - March 6, 2024 Category: Genetics & Stem Cells Authors: Zeming Bai Dapeng Zhou Kai Tao Feng Lin Hongyi Wang Haiwei Sun Ruidi Liu Zhe Li Source Type: research
Immunogenic Cell Death-related Signature Evaluates the Tumor Microenvironment and Predicts the Prognosis in Diffuse Large B-Cell Lymphoma
In conclusion, the present study provides novel insights into the role of ICD in DLBCL by identifying a new biomarker for the disease and may have implications for the development of immune-targeted therapies for the tumor.PMID:38446321 | DOI:10.1007/s10528-024-10697-6 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 6, 2024 Category: Genetics & Stem Cells Authors: Shengqiang Huang Wenbin Liu Qiuling Zhao Ting Chen Ruyi Huang Liangliang Dong Zilin Nian Lin Yang Source Type: research
The Role of MicroRNA-206 in the Regulation of Diabetic Wound Healing via Hypoxia-Inducible Factor 1-Alpha
This study aimed to investigate the abnormal expression of miRNAs in diabetic wound healing and the potential therapeutic role of modulating the miR-206/HIF-1α pathway. MicroRNA assays were used to identify differentially expressed miRNAs in diabetic wound sites and adjacent areas. In vitro models and a rat diabetic model were established to evaluate the effects of miR-206 on HIF-1α regulation and wound healing. The study revealed differential expression of miR-206 in diabetic wound tissues, its interaction with HIF-1α, and the inhibitory effect of miR-206 on cell growth under high glucose conditions. Modulating the miR...
Source: Biochemical Genetics - March 6, 2024 Category: Genetics & Stem Cells Authors: Zeming Bai Dapeng Zhou Kai Tao Feng Lin Hongyi Wang Haiwei Sun Ruidi Liu Zhe Li Source Type: research
Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells are not Really the Same: Investigating the Differences in Their Immunomodulatory, Migratory, and Adhesive Profile
In this study, we compared the levels of major immunomodulatory, adhesive, and migratory factors in human BM-MSCs and AD-MSCs under normal conditions, which will help determine the suitability and specificity of each type for certain therapeutic applications. WST1 assay and fluorescent assay SUC-LLVY-AMC were used to measure MSC proliferation and 26S proteasome activity, respectively. Western blotting, ELISA Assays, and bright field live imaging were also used. AD-MSCs and BM-MSCs exhibited similar morphology and proliferation rate. A significantly higher 26S proteasome activity was detected in AD-MSCs than in BM-MSCs. Lev...
Source: Biochemical Genetics - March 5, 2024 Category: Genetics & Stem Cells Authors: Ejlal Abu-El-Rub Ramada R Khaswaneh Fatimah A Almahasneh Rawan Almazari Ayman Alzu'bi Source Type: research
Novel Diagnostic Biomarker BST2 Identified by Integrated Transcriptomics Promotes the Development of Endometriosis via the TNF- α/NF-κB Signaling Pathway
Biochem Genet. 2024 Mar 5. doi: 10.1007/s10528-024-10666-z. Online ahead of print.ABSTRACTEndometriosis (EMS) is a common gynecological condition with apparent heterogeneity, lack of diagnostic markers, and unclear pathogenesis. A series of bioinformatics methods were employed to explore EMS's pathological mechanisms and potential biomarkers by analyzing the combined datasets of EMS (GSE7305, GSE7307, GSE58198, E-MTAB-694), which included 34 normal, 127 eutopic, and 46 ectopic endometrium samples. Then, wet-laboratory experiments (including Western blot, qRT-PCR, and Immunohistochemistry, Immunofluorescence, CCK-8, EdU, Wo...
Source: Biochemical Genetics - March 5, 2024 Category: Genetics & Stem Cells Authors: Li Jiang Sixue Wang Xiaomeng Xia Tingting Zhang Xi Wang Fei Zeng Jiezhi Ma Xiaoling Fang Source Type: research
GhCOL2 Positively Regulates Flowering by Activating the Transcription of GhHD3A in Upland Cotton (Gossypium hirsutum L.)
In this study, a yeast one-hybrid and dual-LUC expression assays were used to elucidate the molecular mechanism through which GhCOL2 regulates the transcription of GhHD3A. RT-qPCR was used to examine the expression of GhCOL2 and GhHD3A. Our findings reveal that GhCOL2 directly binds to CCACA cis-elements and atypical CORE (TGTGTATG) cis-elements in the promoter regions of HEADING DATE 3 A (HD3A), thereby activating GhHD3A transcription. Notably, GhCOL2 and GhHD3A exhibited high expression levels in the adult stage and low levels in the juvenile stage. Interestingly, the expression of GhCOL2 and GhHD3A varied significant be...
Source: Biochemical Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Xiaoyu Yin Ye Liu Hang Zhao Qi Su Juan Zong Xueying Zhu Ying Bao Source Type: research
The Combinatorial Effect of Ad-IL-24 and Ad-HSV-tk/GCV on Tumor Size, Autophagy, and UPR Mechanisms in Multiple Myeloma Mouse Model
This study aimed to investigate the effects of combination of Adenovector-carrying interleukin-24 and herpes simplex virus 1 thymidine kinase/ganciclovir on tumor growth, autophagy, and unfolded protein response mechanisms in mouse model of multiple myeloma. Six groups of mice, including Ad-HSV-tk/GCV, Ad-IL-24, Ad-HSV-tk/IL-24, Ad-GFP, and positive and negative controls, were investigated, and each group was injected every 72 h. The tumor size was measured several times. The expression of LC3B evaluated through western blotting and ASK-1, CHOP, Caspase-3, and ATF-6 genes in the UPR and apoptosis pathways were also analyze...
Source: Biochemical Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Shima Poorghobadi Seyed Younes Hosseini Seyed Mehdi Sadat Asghar Abdoli Shiva Irani Kazem Baesi Source Type: research
Associations Between BLG, CSN3, DGAT1, GH, PIT1, and PRL Gene Polymorphisms and Milk Production Traits in Holstein Dairy Cows: A Meta-Analysis
Biochem Genet. 2024 Mar 4. doi: 10.1007/s10528-024-10706-8. Online ahead of print.ABSTRACTThe current meta-analysis was employed to combine the results of multiple studies into a single estimate related to B-LG, CSN3, DGAT1, PRL, GH, and PIT1 gene polymorphisms and their effects on milk production traits. The purpose of this meta-analysis was to investigate associations between B-LG, CSN3, DGAT1, GH, PIT1, and PRLgene polymorphisms with milk production traits in Holstein dairy cows. An extensive search was done from 2002 to 2022 year. Statistical analyses were performed by using Stata 11.2 software. Genetic models viz codo...
Source: Biochemical Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Memis Ozdemir Zekrullah Motmain Kubra Ekinci Esranur Sayg ılı Source Type: research
Comprehensive Analyses of PANoptosome with Potential Implications in Cancer Prognosis and Immunotherapy
In conclusion, our study suggests that targeting PANoptosome components and modulating PANoptosis may hold tremendous therapeutic potential in the context of cancer.PMID:38436818 | DOI:10.1007/s10528-024-10687-8 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Yonghua Cai Heng Xiao Qixiong Zhou Jie Lin Xianqiu Liang Wei Xu Yongfu Cao Xian Zhang Hai Wang Source Type: research
Curcumol Attenuates Portal Hypertension and Collateral Shunting Via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats
Biochem Genet. 2024 Mar 4. doi: 10.1007/s10528-024-10684-x. Online ahead of print.ABSTRACTLiver cirrhosis can cause disturbances in blood circulation in the liver, resulting in impaired portal blood flow and ultimately increasing portal venous pressure. Portal hypertension induces portal-systemic collateral formation and fatal complications. Extrahepatic angiogenesis plays a crucial role in the development of portal hypertension. Curcumol is a sesquiterpenoid derived from the rhizome of Curcumae Rhizoma and has been confirmed to alleviate liver fibrosis by inhibiting angiogenesis. Therefore, our study was designed to explo...
Source: Biochemical Genetics - March 4, 2024 Category: Genetics & Stem Cells Authors: Xinyuan Wang Juan Li Jiao Nong Xin Deng Yiping Chen Peibin Wu Xiabing Huang Source Type: research
