Genetic Variations Related to Angiotensin II Production and Risk for Basal Cell Carcinoma
Biochem Genet. 2024 Mar 28. doi: 10.1007/s10528-024-10746-0. Online ahead of print.ABSTRACTBasal cell carcinoma (BCC) is the most prevalent human neoplasm, with constantly increasing annual incidence. Despite its slow growth, BCC is locally invasive and, if left untreated, can cause severe complications, including metastasis and death. The renin-angiotensin system (RAS) plays a key role in electrolyte balance, atrial pressure, tissue development, homeostasis, and inflammation, but also in cancer development. After binding to its type 1 receptor (AT1R), angiotensin II (ANGII), the system's principal hormonal effector, regul...
Source: Biochemical Genetics - March 28, 2024 Category: Genetics & Stem Cells Authors: Iphigenia Gintoni Michael Mastrogeorgiou Veronica Papakosta Stavros Vassiliou Christos Yapijakis Source Type: research
MiR-374a/b-5p Suppresses Cell Growth in Papillary Thyroid Carcinoma Through Blocking Exosomal ANXA1-Induced Macrophage M2 Polarization
Biochem Genet. 2024 Mar 27. doi: 10.1007/s10528-024-10747-z. Online ahead of print.ABSTRACTPapillary thyroid carcinoma (PTC), comprising 85% of all thyroid cancers, is an epithelial malignancy. The potential for malignant transformation in normal cells by thyroid cancer cells via exosomal Annexin A1 (ANXA1) delivery is investigated in this study. Our aim is to determine the impact of PTC cells on macrophage polarization through exosomal ANXA1 secretion and its implications for tumor progression. Exosomes in PTC cells were examined using transmission electron microscopy, exosome labeling, and nanoparticle tracking analysis....
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Sanbao Liu Source Type: research
Integration of Single-Cell and Bulk RNA-seq Data to Identify the Cancer-Associated Fibroblast Subtypes and Risk Model in Glioma
Biochem Genet. 2024 Mar 27. doi: 10.1007/s10528-024-10751-3. Online ahead of print.ABSTRACTCancer-associated fibroblasts (CAFs) are an important component of the stroma. Studies showed that CAFs were pivotally in glioma progression which have long been considered a promising therapeutic target. Therefore, the identification of prognostic CAF markers might facilitate the development of novel diagnostic and therapeutic approaches. A total of 1333 glioma samples were obtained from the TCGA and CGGA datasets. The EPIC, MCP-counter, and xCell algorithms were used to evaluate the relative proportion of CAFs in glioma. CAF marker...
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Xiuwei Yan Xin Gao Jiawei Dong Fang Wang Xiaoyan Jiang Xueyan Hu Jiheng Zhang Nan Wang Lei Xu Zhihui Liu Shaoshan Hu Hongtao Zhao Source Type: research
Trimethylamine-N-oxide, a New Risk Factor for Non-alcoholic Fatty Liver Disease Changes the Expression of miRNA-34a, and miRNA-122 in the Fatty Liver Cell Model
This study investigated the expression of miRNA-34a, miRNA-122, and miRNA-192 in the fatty liver cell model treated with different concentrations of TMAO. A fatty liver cell model was developed by exposing HepG2 cells to a mixture of palmitate and oleate in a ratio of 1:2 at a final concentration of 1200 μM for 24 h. The confirmed fatty liver cells were treated with 37.5, 75, 150, and 300 μM of TMAO for 24 h. RT-qPCR was used to quantify the expression of microRNAs in a cellular model. The cellular expression of all microRNAs was significantly higher in treated fatty liver cells compared to normal HepG2 cells (P < 0.0...
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Zhila Bahramirad Mohammad Raman Moloudi Mohammad Moradzad Alina Abdollahi Zakaria Vahabzadeh Source Type: research
MiR-374a/b-5p Suppresses Cell Growth in Papillary Thyroid Carcinoma Through Blocking Exosomal ANXA1-Induced Macrophage M2 Polarization
Biochem Genet. 2024 Mar 27. doi: 10.1007/s10528-024-10747-z. Online ahead of print.ABSTRACTPapillary thyroid carcinoma (PTC), comprising 85% of all thyroid cancers, is an epithelial malignancy. The potential for malignant transformation in normal cells by thyroid cancer cells via exosomal Annexin A1 (ANXA1) delivery is investigated in this study. Our aim is to determine the impact of PTC cells on macrophage polarization through exosomal ANXA1 secretion and its implications for tumor progression. Exosomes in PTC cells were examined using transmission electron microscopy, exosome labeling, and nanoparticle tracking analysis....
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Sanbao Liu Source Type: research
Integration of Single-Cell and Bulk RNA-seq Data to Identify the Cancer-Associated Fibroblast Subtypes and Risk Model in Glioma
Biochem Genet. 2024 Mar 27. doi: 10.1007/s10528-024-10751-3. Online ahead of print.ABSTRACTCancer-associated fibroblasts (CAFs) are an important component of the stroma. Studies showed that CAFs were pivotally in glioma progression which have long been considered a promising therapeutic target. Therefore, the identification of prognostic CAF markers might facilitate the development of novel diagnostic and therapeutic approaches. A total of 1333 glioma samples were obtained from the TCGA and CGGA datasets. The EPIC, MCP-counter, and xCell algorithms were used to evaluate the relative proportion of CAFs in glioma. CAF marker...
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Xiuwei Yan Xin Gao Jiawei Dong Fang Wang Xiaoyan Jiang Xueyan Hu Jiheng Zhang Nan Wang Lei Xu Zhihui Liu Shaoshan Hu Hongtao Zhao Source Type: research
Trimethylamine-N-oxide, a New Risk Factor for Non-alcoholic Fatty Liver Disease Changes the Expression of miRNA-34a, and miRNA-122 in the Fatty Liver Cell Model
This study investigated the expression of miRNA-34a, miRNA-122, and miRNA-192 in the fatty liver cell model treated with different concentrations of TMAO. A fatty liver cell model was developed by exposing HepG2 cells to a mixture of palmitate and oleate in a ratio of 1:2 at a final concentration of 1200 μM for 24 h. The confirmed fatty liver cells were treated with 37.5, 75, 150, and 300 μM of TMAO for 24 h. RT-qPCR was used to quantify the expression of microRNAs in a cellular model. The cellular expression of all microRNAs was significantly higher in treated fatty liver cells compared to normal HepG2 cells (P < 0.0...
Source: Biochemical Genetics - March 27, 2024 Category: Genetics & Stem Cells Authors: Zhila Bahramirad Mohammad Raman Moloudi Mohammad Moradzad Alina Abdollahi Zakaria Vahabzadeh Source Type: research
The Hsa_circ_0105558/miR-182-5p/ATF6 Cascade Affects H < sub > 2 < /sub > O < sub > 2 < /sub > -Triggered Oxidative Damage and Apoptosis of Human Lens Epithelial Cells
Biochem Genet. 2024 Mar 26. doi: 10.1007/s10528-024-10753-1. Online ahead of print.ABSTRACTAge-related cataract (ARC) is the prevalent cause of useful vision loss. Circular RNAs are related to ARC pathogenesis partly through their competing endogenous RNA (ceRNA) activity. Herein, we defined the action of hsa_circ_0105558 in hydrogen peroxide (H2O2)-driven apoptosis and oxidative damage in human lens epithelial SRA01/04 cells. Hsa_circ_0105558, microRNA (miR)-182-5p and activating transcription factor 6 (ATF6) were evaluated by a qRT-PCR or immunoblotting method. The hsa_circ_0105558/miR-182-5p and miR-182-5p/ATF6 relation...
Source: Biochemical Genetics - March 26, 2024 Category: Genetics & Stem Cells Authors: Wei Mao Zhe Zhang Source Type: research
Role of C/EBP Homologous Protein in Vascular Stenosis After Carotid Artery Injury
In conclusion, CHOP plays a crucial role in endothelial injury, proliferation, and VSMCs migration during carotid artery injury, serving as a pivotal regulator in post-injury fibrous plaque formation and vascular remodeling. Statins emerge as protectors of endothelial cells, restraining VSMCs migration by modulating CHOP expression.PMID:38526708 | DOI:10.1007/s10528-024-10713-9 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 25, 2024 Category: Genetics & Stem Cells Authors: Lin Teng Qin Qin Zi-Yi Zhou Fei Zhou Cun-Yu Cao Chao He Jia-Wang Ding Jian Yang Source Type: research
Constructing a Novel Amino Acid Metabolism Signature: A New Perspective on Pheochromocytoma Diagnosis, Immune Landscape, and Immunotherapy
This study aimed to build a risk model using amino acid metabolism-related genes, enhancing PGPG diagnosis and treatment decisions. We analyzed RNA-sequencing data from the PCPG cohort in the GEO dataset as our training set and validated our findings using the TCGA dataset and an additional clinical cohort. WGCNA and LASSO were utilized to identify hub genes and develop risk prediction models. The single-sample gene set enrichment analysis, MCPCOUNTER, and ESTIMATE algorithm calculated the relationship between amino acid metabolism and immune cell infiltration in PCPG. The TIDE algorithm predicted the immunotherapy efficac...
Source: Biochemical Genetics - March 25, 2024 Category: Genetics & Stem Cells Authors: Zechen Yan Yongkun Luan Yu Wang Yilin Ren Zhiyuan Li Luyang Zhao Linnuo Shen Xiaojie Yang Tonghu Liu Yukui Gao Weibo Sun Source Type: research
Knocking Down HN1 Blocks Helicobacter pylori-Induced Malignant Phenotypes in Gastric Mucosal Cells and Inhibits Gastric Cancer Cell Proliferation, Cytoskeleton Remodeling, and Migration
Biochem Genet. 2024 Mar 25. doi: 10.1007/s10528-024-10731-7. Online ahead of print.ABSTRACTHelicobacter pylori (H. pylori) is implicated in the aberrant proliferation and malignant transformation of gastric mucosal cells, heightening the risk of gastric cancer (GC). HN1 is involved in the development of various tumors. However, precise mechanistic underpinnings of HN1 promoting GC progression in H. pylori remain elusive. The study collected 79 tissue samples of GC patients, including 47 with H. pylori-positive GC and 32 H. pylori-negative controls. Using human gastric epithelial cells (GES-1) and human gastric adenocarcino...
Source: Biochemical Genetics - March 25, 2024 Category: Genetics & Stem Cells Authors: Ying Huang Xiaofei Wang Hao Liu Xiangkun Meng Hua Yin Ruirui Hou Wan Lin Xu Zhang Jun Ma Xiaoxu Zhang Feixiong Zhang Yu Miao Source Type: research
Potential Impact of SOD2 (rs4880; p.Val16Ala) Variant with the Susceptibility for Childhood Bronchial Asthma
Biochem Genet. 2024 Mar 24. doi: 10.1007/s10528-024-10742-4. Online ahead of print.ABSTRACTOxidative stress is a sophisticated situation that orignates from the accumulation of reactive free radicals within cellular compartments. The antioxidant mechanism of the MnSOD enzyme facilitates the removal of these lethal oxygen species from cellular components. The main goal of this pertained work is to study the contribution of the SOD2 (rs4880; p.Val16Ala) variant to the development of bronchial asthma among children. The study's design was carried out based on a total of 254 participants including 127 asthmatic children (91 at...
Source: Biochemical Genetics - March 24, 2024 Category: Genetics & Stem Cells Authors: Nahla H Anber Hanaa Elsayed Ahmed Shahin Heba K Badawy Enas A Oraby Sameh A Mohammed Esraa Ibrahim A Shaaban Zeinab Rizk Attia Shereen Mohamed Mona Farag Shabana Mohamed Adel El-Eshmawy Riham Elsayed Afaf M Elsaid Adel I Alalawy Rami M Elshazli Source Type: research
Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control
Biochem Genet. 2024 Mar 24. doi: 10.1007/s10528-024-10714-8. Online ahead of print.ABSTRACTBladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. One of its mechanisms of action is the regulation of RECK, a tumor suppressor gene responsible for inhibiting metalloproteinase...
Source: Biochemical Genetics - March 24, 2024 Category: Genetics & Stem Cells Authors: Paulo Rodolfo Moraes Dos Santos Paulo Ricardo da Silva Gomes Poliana Rom ão Feres Camargo Maluf Vanessa Ribeiro Guimar ães Patr ícia Candido Guilherme Lopes Gon çalves Juliana Alves de Camargo Gabriel Arantes Dos Santos Iran Silva Katia Ramos Moreira Source Type: research
Potential Impact of SOD2 (rs4880; p.Val16Ala) Variant with the Susceptibility for Childhood Bronchial Asthma
Biochem Genet. 2024 Mar 24. doi: 10.1007/s10528-024-10742-4. Online ahead of print.ABSTRACTOxidative stress is a sophisticated situation that orignates from the accumulation of reactive free radicals within cellular compartments. The antioxidant mechanism of the MnSOD enzyme facilitates the removal of these lethal oxygen species from cellular components. The main goal of this pertained work is to study the contribution of the SOD2 (rs4880; p.Val16Ala) variant to the development of bronchial asthma among children. The study's design was carried out based on a total of 254 participants including 127 asthmatic children (91 at...
Source: Biochemical Genetics - March 24, 2024 Category: Genetics & Stem Cells Authors: Nahla H Anber Hanaa Elsayed Ahmed Shahin Heba K Badawy Enas A Oraby Sameh A Mohammed Esraa Ibrahim A Shaaban Zeinab Rizk Attia Shereen Mohamed Mona Farag Shabana Mohamed Adel El-Eshmawy Riham Elsayed Afaf M Elsaid Adel I Alalawy Rami M Elshazli Source Type: research
Enhancing RECK Expression Through miR-21 Inhibition: A Promising Strategy for Bladder Carcinoma Control
Biochem Genet. 2024 Mar 24. doi: 10.1007/s10528-024-10714-8. Online ahead of print.ABSTRACTBladder carcinoma (BC) is the tenth most frequent malignancy worldwide, with high morbidity and mortality rates. Despite recent treatment advances, high-grade BC and muscle-invasive BC present with significant progression and recurrence rates, urging the need for alternative treatments. The microRNA-21 (miR-21) has superexpression in many malignancies and is associated with cellular invasion and progression. One of its mechanisms of action is the regulation of RECK, a tumor suppressor gene responsible for inhibiting metalloproteinase...
Source: Biochemical Genetics - March 24, 2024 Category: Genetics & Stem Cells Authors: Paulo Rodolfo Moraes Dos Santos Paulo Ricardo da Silva Gomes Poliana Rom ão Feres Camargo Maluf Vanessa Ribeiro Guimar ães Patr ícia Candido Guilherme Lopes Gon çalves Juliana Alves de Camargo Gabriel Arantes Dos Santos Iran Silva Katia Ramos Moreira Source Type: research
