Cuproptosis-Related Gene FDX1 Suppresses the Growth and Progression of Colorectal Cancer by Retarding EMT Progress
In conclusion, FDX1 inhibited the growth and progression of CRC by inhibiting EMT progress. This discovery hinted that FDX1 may act as an effective candidate for CRC treatment.PMID:38520567 | DOI:10.1007/s10528-024-10784-8 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 23, 2024 Category: Genetics & Stem Cells Authors: Chao Wang Jingjing Guo Yun Zhang Shusheng Zhou Bing Jiang Source Type: research
Cuproptosis-Related Gene FDX1 Suppresses the Growth and Progression of Colorectal Cancer by Retarding EMT Progress
In conclusion, FDX1 inhibited the growth and progression of CRC by inhibiting EMT progress. This discovery hinted that FDX1 may act as an effective candidate for CRC treatment.PMID:38520567 | DOI:10.1007/s10528-024-10784-8 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 23, 2024 Category: Genetics & Stem Cells Authors: Chao Wang Jingjing Guo Yun Zhang Shusheng Zhou Bing Jiang Source Type: research
XRCC1 Polymorphisms p.Arg194Trp, p.Arg280His, and p.Arg399Gln, Polycyclic Aromatic Hydrocarbons, and Infertility: A Case-Control and In Silico Study
Biochem Genet. 2024 Mar 21. doi: 10.1007/s10528-024-10743-3. Online ahead of print.ABSTRACTXRCC1 is involved in repair of single-strand breaks generated by mutagenic exposure. Polymorphisms within XRCC1 affect its ability to efficiently repair DNA damage. Polycyclic aromatic hydrocarbons or PAHs are genotoxic compounds which form bulky DNA adducts that are linked with infertility. Few reports suggest combined role of XRCC1 polymorphisms and PAHs in infertility. Present study investigates association of three XRCC1 polymorphisms (p.Arg194Trp, p.Arg280His, p.Arg399Gln) with male and female infertility in a North-West Indian ...
Source: Biochemical Genetics - March 22, 2024 Category: Genetics & Stem Cells Authors: Jatinder Singh Sahota Kamlesh Guleria Vasudha Sambyal Source Type: research
XRCC1 Polymorphisms p.Arg194Trp, p.Arg280His, and p.Arg399Gln, Polycyclic Aromatic Hydrocarbons, and Infertility: A Case-Control and In Silico Study
Biochem Genet. 2024 Mar 21. doi: 10.1007/s10528-024-10743-3. Online ahead of print.ABSTRACTXRCC1 is involved in repair of single-strand breaks generated by mutagenic exposure. Polymorphisms within XRCC1 affect its ability to efficiently repair DNA damage. Polycyclic aromatic hydrocarbons or PAHs are genotoxic compounds which form bulky DNA adducts that are linked with infertility. Few reports suggest combined role of XRCC1 polymorphisms and PAHs in infertility. Present study investigates association of three XRCC1 polymorphisms (p.Arg194Trp, p.Arg280His, p.Arg399Gln) with male and female infertility in a North-West Indian ...
Source: Biochemical Genetics - March 22, 2024 Category: Genetics & Stem Cells Authors: Jatinder Singh Sahota Kamlesh Guleria Vasudha Sambyal Source Type: research
Design of an In Vitro Model for Epithelial-to-Mesenchymal Transition in Gastric Cancer
Biochem Genet. 2024 Mar 20. doi: 10.1007/s10528-024-10668-x. Online ahead of print.ABSTRACTEpithelial-to-mesenchymal transition (EMT) is a developmental program that plays a vital role in gastric cancer, including aspects of tumor progression, the metastatic process, and resistance to treatment. Here, we have designed an in vitro model that mimics the features of EMT as observed in gastric cancer. The results showed that both migration and invasion were enhanced in gastric cancer cells with Brachyury overexpression. Additionally, the expression of IL-8 increased, while IL-8RA and IL-8RB levels significantly decreased in th...
Source: Biochemical Genetics - March 21, 2024 Category: Genetics & Stem Cells Authors: Yuanhui Zhang Ling Bi Quanyao Li Liqiu Yao Xiao Wang Hui Liu Jun Shi Source Type: research
Hsa-miR-92b-3p Targeting FHL2 to Enhance Radiosensitivity of Nasopharyngeal Carcinoma
In this study, we found that the expression level of miR-92b-3p was significantly higher in radiotherapy-sensitive NPC patients than in radiotherapy-resistant patients. High expression of miR-92b-3p was associated with good prognosis in patients with NPC, and high expression of FHL2 was associated with poor prognosis in patients with NPC. It was predicted that miR-92b-3p could directly target and bind FHL2. Overexpression of miR-92b-3p significantly inhibited FHL2 expression at the mRNA as well as protein levels, while inhibition of miR-92b-3p expression significantly upregulated FHL2 expression. Overexpression of miR-92b-...
Source: Biochemical Genetics - March 21, 2024 Category: Genetics & Stem Cells Authors: Can Zeng Shuangni Duan Lin Zhao Jing Jiang Source Type: research
SOX21-AS1 Augmented Cervical Cancer Growth by Triggering FZD3 to Activate the Wnt/ β-Catenin Signaling Pathway
Biochem Genet. 2024 Mar 21. doi: 10.1007/s10528-024-10770-0. Online ahead of print.ABSTRACTLong non-coding RNA (LncRNA) SOX21-AS1 has been reported that it plays an important role in biological processes of several cancers. However, how it functions in cervical cancer (CC) still remain unclear. This investigation seeks to explore the impact of SOX21-AS1 on CC cell proliferation, invasion and migration and its association to the FZD3 and the Wnt/β-catenin signaling pathway. SOX21-AS1 expression levels were detected using real-time quantitative PCR in 20 cases of cervical cancer together with its adjacent tissues and severa...
Source: Biochemical Genetics - March 21, 2024 Category: Genetics & Stem Cells Authors: Yanfang Zhi Peipei Du Ya Li Hui Liu Tao Jiang Xin Zhao Xiaofu Li Source Type: research
Molecular Characterization and Seasonal Variation in Expression of HSP70.1 Gene in Gangatiri Cattle and Its Comparison with Buffalo
Biochem Genet. 2024 Mar 18. doi: 10.1007/s10528-024-10739-z. Online ahead of print.ABSTRACTUnder tropical climate heat stress is a major challenge for livestock production. HSP70.1 is a ubiquitously expressed protein maintaining cellular machinery through proper folding of denatured proteins and prevents cellular apoptosis and protect cell from heat stress. Therefore, present investigation was undertaken to explore genetic variability in HSP70.1 gene in Gangatiri cattle, its comparison with buffalo sequences and differential expression in different season. The allelic variant was identified by sequencing amplified PCR prod...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Shanker Dayal Birendra Kumar Rajni Kumari Jyoti Kumar Pradeep Kumar Ray P C Chandran Amitava Dey Source Type: research
RBM24 Suppresses the Tumorigenesis of Glioblastoma by Stabilizing LATS1 mRNA
The objective of this study is to uncover the role of RBM24 in GBM and understand the underlying mechanism. The expression of RBM24 in GBM was initially analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA). Subsequently, the RBM24 expression levels in clinical samples of GBM were examined, and the survival curves of GBM patients were plotted based on high- and low-expression levels of RBM24 using Kaplan-Meier (KM) plotter. In addition, RBM24 knockdown cell lines and overexpression vectors were created to assess the effects on proliferation, apoptosis, and invasion abilities. Finally, the binding level ...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Xuewen Lu Yong Xie Guolin Ding Wei Sun Hao Ye Source Type: research
Integrating Network Pharmacology and In Vitro Experimental Verification Revealing Bushenhuoxue Recipe Against Intrauterine Adhesions via PI3K-AKT Signaling Pathway
Biochem Genet. 2024 Mar 19. doi: 10.1007/s10528-024-10732-6. Online ahead of print.ABSTRACTWe aimed to investigate therapeutic effect of Bushenhuoxue recipe in intrauterine adhesions (IUA) and explore the underlying molecular mechanism via integrating network pharmacology and in vitro experimental verification. The active compounds and gene targets of Bushenhuoxue recipe were screened in the TCMSP database and the IUA-related genes were identified using GeneCards database by the keyword "Intrauterine adhesions". Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the unde...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Lingli Wang Lingzi Zhuang Yajing Lu Jin Jia Cailing Chen Ying Zhang Zhejing Hong Xiaohong Wang Source Type: research
Molecular Cloning and Functional Analysis of Secretory Phospholipase A < sub > 2 < /sub > from Apostichopus japonicus
This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2s from starfish have the typical domains of the Group IB sPLA2. And additional amino acid sequences were found around the β-Wing, which is different from the Group IB sPLA2. These results showed that AjPLA2 and sPLA2s from starfish all belong to a new group in the Group I sPLA2 family. AjPLA2 is widely distributed in sea cuc...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Cheng Li Lili Yang Zhongyun Zhang Ying Liu Xu Li Kai Yang Ming Chen Source Type: research
Molecular Characterization and Seasonal Variation in Expression of HSP70.1 Gene in Gangatiri Cattle and Its Comparison with Buffalo
Biochem Genet. 2024 Mar 18. doi: 10.1007/s10528-024-10739-z. Online ahead of print.ABSTRACTUnder tropical climate heat stress is a major challenge for livestock production. HSP70.1 is a ubiquitously expressed protein maintaining cellular machinery through proper folding of denatured proteins and prevents cellular apoptosis and protect cell from heat stress. Therefore, present investigation was undertaken to explore genetic variability in HSP70.1 gene in Gangatiri cattle, its comparison with buffalo sequences and differential expression in different season. The allelic variant was identified by sequencing amplified PCR prod...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Shanker Dayal Birendra Kumar Rajni Kumari Jyoti Kumar Pradeep Kumar Ray P C Chandran Amitava Dey Source Type: research
RBM24 Suppresses the Tumorigenesis of Glioblastoma by Stabilizing LATS1 mRNA
The objective of this study is to uncover the role of RBM24 in GBM and understand the underlying mechanism. The expression of RBM24 in GBM was initially analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA). Subsequently, the RBM24 expression levels in clinical samples of GBM were examined, and the survival curves of GBM patients were plotted based on high- and low-expression levels of RBM24 using Kaplan-Meier (KM) plotter. In addition, RBM24 knockdown cell lines and overexpression vectors were created to assess the effects on proliferation, apoptosis, and invasion abilities. Finally, the binding level ...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Xuewen Lu Yong Xie Guolin Ding Wei Sun Hao Ye Source Type: research
Integrating Network Pharmacology and In Vitro Experimental Verification Revealing Bushenhuoxue Recipe Against Intrauterine Adhesions via PI3K-AKT Signaling Pathway
Biochem Genet. 2024 Mar 19. doi: 10.1007/s10528-024-10732-6. Online ahead of print.ABSTRACTWe aimed to investigate therapeutic effect of Bushenhuoxue recipe in intrauterine adhesions (IUA) and explore the underlying molecular mechanism via integrating network pharmacology and in vitro experimental verification. The active compounds and gene targets of Bushenhuoxue recipe were screened in the TCMSP database and the IUA-related genes were identified using GeneCards database by the keyword "Intrauterine adhesions". Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the unde...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Lingli Wang Lingzi Zhuang Yajing Lu Jin Jia Cailing Chen Ying Zhang Zhejing Hong Xiaohong Wang Source Type: research
Molecular Cloning and Functional Analysis of Secretory Phospholipase A < sub > 2 < /sub > from Apostichopus japonicus
This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2s from starfish have the typical domains of the Group IB sPLA2. And additional amino acid sequences were found around the β-Wing, which is different from the Group IB sPLA2. These results showed that AjPLA2 and sPLA2s from starfish all belong to a new group in the Group I sPLA2 family. AjPLA2 is widely distributed in sea cuc...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Cheng Li Lili Yang Zhongyun Zhang Ying Liu Xu Li Kai Yang Ming Chen Source Type: research
