Linker Histone H1.4 Inhibits the Growth, Migration and EMT Process of Non-Small Cell Lung Cancer by Regulating ERK1/2 Expression
In this study, we found that overexpression of H1.4 significantly inhibited the cell viability, migration, invasion and epithelial-mesenchymal transition (EMT) processes, whereas silencing H1.4 by shRNA knockdown promoted these processes in NSCLC cell lines A549 and H1299. We further showed that H1.4 overexpression reduced ERK1/2 expression or its phosphorylation levels, while H1.4 knockdown increased ERK1/2 expression or phosphorylation levels in NSCLC. Furthermore, we demonstrated that H1.4 bound to the promoter of ERK1/2, and acted as a transcriptional suppressor to inhibit ERK1/2 expression in A549 or H1299 cells. Impo...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Qian Chen Mengqi Yang Xinyue Duan Jie Zhang Fan Shi Rong Chen Yong Li Source Type: research
DR6 Augments Colorectal Cancer Cell Growth, Invasion, and Stemness by Activating AKT/NF- κB Pathway
This study aims to elucidate the role and mechanisms of Death Receptor 6 (DR6), a member of the tumor necrosis factor receptor superfamily, in the malignant progression of colorectal cancer (CRC). The association of DR6 expression levels and CRC patient survival was examined using the CRC cohort data from GEPIA database. The functional role of DR6 in CRC cells was investigated by performing loss-of-function and gain-of-function experiments based on CCK-8 proliferation assay, transwell migration and invasion assay, and sphere-forming assays. Xenograft model of CRC cells in nude mouse was established to evaluate the impact o...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Jing Jia Yisen Huang Qiwei Chen Jianbin Hou Yan Liu Lifeng Xie Xinyu Li Chunkang Yang Source Type: research
Observation of the Effects of Different Enzyme Digestion Time and Enzyme Digestion Methods on the Extraction of Primary Renal Tubular Epithelial Cells from Sprague-Dawley Suckling Mice
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10729-1. Online ahead of print.ABSTRACTRenal tubular epithelial cells are one of the essential functional cells in the kidney. Optimizing the isolation and culture method of primary renal tubular epithelial cells from SD mammary rats provides better experimental materials for renal tubule-related studies, which is essential for studying the pathogenesis of renal diseases, especially diabetic nephropathy and drug screening. SD rat renal tubular epithelial cells were isolated and purified by 2.5-mg/ml collagenase II or 2 mg/ml trypsin + 2.5 mg/ml collagenase II enzymatic di...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Tian Sun Hongguang Chen Zhenwang Zhang Source Type: research
Effect of Application of Autologous Conditioned Serum on Wound Healing in Diabetic Mice Through Inhibition of STING Pathway Activation
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10734-4. Online ahead of print.ABSTRACTSlow wound healing in diabetic patients is a common complication of diabetes. Autologous conditioned serum (ACS) therapy is an emerging and safe biological therapy, and may accelerate the wound healing in diabetes. To investigate the effect of ACS in promoting wound healing in diabetic mice and its possible mechanism. Twenty-four six-week-old male C57BL/6 J mice were selected and divided into 5 groups, including control group (Ctrl), diabetic wound group (DW), ACS treatment group (DW+ACS) and STING pathway validation group (DW+ACS+DM...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Hongxia Li Source Type: research
Assessment of CTLA-4 Gene Expression Levels on CD8+ T Cells in Renal Transplant Patients and Relation with Serum sCTLA-4 Levels
Biochem Genet. 2024 Mar 11. doi: 10.1007/s10528-024-10723-7. Online ahead of print.ABSTRACTCTLA-4 (Cytotoxic T Lymphocyte Antigen-4) is an immune regulator molecule that is expressed on a variety of immune cells, including CD4+ and CD8+ T cells. After realizing the significance of this regulator molecule, researchers began to concentrate on its activation or inhibition in cancer. Even though there have been some studies on organ transplantation and autoimmunity, the role of the CTLA-4 molecule in renal transplantation has not been demonstrated. The goal of this study was to see how CTLA-4 gene expression and serum sCTLA-4 ...
Source: Biochemical Genetics - March 12, 2024 Category: Genetics & Stem Cells Authors: Burcu Çerçi Alkaç Mustafa Soy öz Melek Pehlivan T ülay Kılıçaslan Ayna Erhan Tatar H İlayhan Karahan Çöven Mehmet Tanr ısev İbrahim Pirim Source Type: research
BPIFB1, Serving as a Downstream Effector of EBV-miR-BART4, Blocks Immune Escape of Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression
This study provided a comprehensive understanding of the molecular mechanism for the upstream and downstream pathway of BPIFB1 related with immune escape, indicating a novel approach for the treatment of NPC.PMID:38467887 | DOI:10.1007/s10528-024-10719-3 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 12, 2024 Category: Genetics & Stem Cells Authors: Jiaodi Cai Li Xiao Jiao Liu Dan Wang Yadong Zhou Zhiming Liao Guoqun Chen Source Type: research
Assessment of CTLA-4 Gene Expression Levels on CD8+ T Cells in Renal Transplant Patients and Relation with Serum sCTLA-4 Levels
Biochem Genet. 2024 Mar 11. doi: 10.1007/s10528-024-10723-7. Online ahead of print.ABSTRACTCTLA-4 (Cytotoxic T Lymphocyte Antigen-4) is an immune regulator molecule that is expressed on a variety of immune cells, including CD4+ and CD8+ T cells. After realizing the significance of this regulator molecule, researchers began to concentrate on its activation or inhibition in cancer. Even though there have been some studies on organ transplantation and autoimmunity, the role of the CTLA-4 molecule in renal transplantation has not been demonstrated. The goal of this study was to see how CTLA-4 gene expression and serum sCTLA-4 ...
Source: Biochemical Genetics - March 12, 2024 Category: Genetics & Stem Cells Authors: Burcu Çerçi Alkaç Mustafa Soy öz Melek Pehlivan T ülay Kılıçaslan Ayna Erhan Tatar H İlayhan Karahan Çöven Mehmet Tanr ısev İbrahim Pirim Source Type: research
BPIFB1, Serving as a Downstream Effector of EBV-miR-BART4, Blocks Immune Escape of Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression
This study provided a comprehensive understanding of the molecular mechanism for the upstream and downstream pathway of BPIFB1 related with immune escape, indicating a novel approach for the treatment of NPC.PMID:38467887 | DOI:10.1007/s10528-024-10719-3 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 12, 2024 Category: Genetics & Stem Cells Authors: Jiaodi Cai Li Xiao Jiao Liu Dan Wang Yadong Zhou Zhiming Liao Guoqun Chen Source Type: research
Gene Rearrangements in the Mitochondrial Genome of Gonatopsis borealis and Onychoteuthis compacta Reveal Their Phylogenetic Implications for Oegopsida
Biochem Genet. 2024 Mar 11. doi: 10.1007/s10528-024-10707-7. Online ahead of print.ABSTRACTThe complete mitochondrial genome provides crucial information for comprehending gene rearrangement, molecular evolution, and phylogenetic analysis. Here, we have determined the complete mitogenome sequence of Gonatopsis borealis and Onychoteuthis compacta for the first time. Their genome sizes were 20,148 bp and 20,491 bp, respectively, including 18 protein-coding genes, COI-COIII, ATP6, and ATP8 are duplicated, 23 transfer RNA genes, and 2 ribosomal RNA (rRNA) genes (12S and 16S rRNA). Specifically, the overall A+T content is 70.69...
Source: Biochemical Genetics - March 11, 2024 Category: Genetics & Stem Cells Authors: Fan Fan Liyi Pei Lihua Jiang Yingying Ye Yifan Liu Bilin Liu Source Type: research
In Silico Prediction of Functional SNPs Interrupting Antioxidant Defense Genes in Relation to COVID-19 Progression
Biochem Genet. 2024 Mar 9. doi: 10.1007/s10528-024-10705-9. Online ahead of print.ABSTRACTThe excessive production of reactive oxygen species and weakening of antioxidant defense system play a pivotal role in the pathogenesis of different diseases. Extensive differences observed among individuals in terms of affliction with cancer, cardiovascular disorders, diabetes, bacterial, and viral infections, as well as response to treatments can be partly due to their genomic variations. In this work, we attempted to predict the effect of SNPs of the key genes of antioxidant defense system on their structure, function, and expressi...
Source: Biochemical Genetics - March 9, 2024 Category: Genetics & Stem Cells Authors: Somayeh Hashemi Sheikhshabani Soudeh Ghafouri-Fard Zeinab Amini-Farsani Parastoo Modarres Sharareh Khazaei Feyzabad Zahra Amini-Farsani Nasibeh Shaygan Mir Davood Omrani Source Type: research
In Silico Prediction of Functional SNPs Interrupting Antioxidant Defense Genes in Relation to COVID-19 Progression
Biochem Genet. 2024 Mar 9. doi: 10.1007/s10528-024-10705-9. Online ahead of print.ABSTRACTThe excessive production of reactive oxygen species and weakening of antioxidant defense system play a pivotal role in the pathogenesis of different diseases. Extensive differences observed among individuals in terms of affliction with cancer, cardiovascular disorders, diabetes, bacterial, and viral infections, as well as response to treatments can be partly due to their genomic variations. In this work, we attempted to predict the effect of SNPs of the key genes of antioxidant defense system on their structure, function, and expressi...
Source: Biochemical Genetics - March 9, 2024 Category: Genetics & Stem Cells Authors: Somayeh Hashemi Sheikhshabani Soudeh Ghafouri-Fard Zeinab Amini-Farsani Parastoo Modarres Sharareh Khazaei Feyzabad Zahra Amini-Farsani Nasibeh Shaygan Mir Davood Omrani Source Type: research
Complete Mitochondrial Genome and Phylogenetic Position of Nurudea zhengii Ren (Insecta, Hemiptera, Aphididae)
Biochem Genet. 2024 Mar 8. doi: 10.1007/s10528-024-10717-5. Online ahead of print.ABSTRACTNurudea zhengii Ren was identified by aphid morphological characteristics as well as the gall shape and host plant species, and placed in the tribe Fordini (Hemiptera, Aphididae, Eriosomatinae). Here, its whole genome was firstly sequenced by a genome-skimming method and its mitochondrial genome (mitogenome) was assembled to examine its genetic variation and phylogenetic position. The complete mitogenome of Nurudea zhengii is 15,392 bp in length, and consists of 13 protein-coding genes, 22 tRNAs, two rRNAs and one D-loop region. The g...
Source: Biochemical Genetics - March 8, 2024 Category: Genetics & Stem Cells Authors: Yuzhen Song Yukang Liang Wenli Ma M James C Crabbe Zhumei Ren Source Type: research
Complete Mitochondrial Genome and Phylogenetic Position of Nurudea zhengii Ren (Insecta, Hemiptera, Aphididae)
Biochem Genet. 2024 Mar 8. doi: 10.1007/s10528-024-10717-5. Online ahead of print.ABSTRACTNurudea zhengii Ren was identified by aphid morphological characteristics as well as the gall shape and host plant species, and placed in the tribe Fordini (Hemiptera, Aphididae, Eriosomatinae). Here, its whole genome was firstly sequenced by a genome-skimming method and its mitochondrial genome (mitogenome) was assembled to examine its genetic variation and phylogenetic position. The complete mitogenome of Nurudea zhengii is 15,392 bp in length, and consists of 13 protein-coding genes, 22 tRNAs, two rRNAs and one D-loop region. The g...
Source: Biochemical Genetics - March 8, 2024 Category: Genetics & Stem Cells Authors: Yuzhen Song Yukang Liang Wenli Ma M James C Crabbe Zhumei Ren Source Type: research
Screening of Genes Associated with Immune Infiltration of Discoid Lupus Erythematosus Based on Weighted Gene Co-expression Network Analysis
Biochem Genet. 2024 Mar 7. doi: 10.1007/s10528-023-10603-6. Online ahead of print.ABSTRACTDiscoid lupus erythematosus (DLE) is a disorder of the immune system commonly seen in women of childbearing age. The pathophysiology and aetiology are still poorly understood, and no cure is presently available. Therefore, there is an urgent need to explore the underlying molecular mechanisms, as well as search for new therapeutic targets. Gene expression data from skin biopsies samples of DLE patients and healthy controls were downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between DLE ...
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Yuru Han Shuang Liu Shuo Shi Yongyong Shu Changlian Lu Xuefeng Gu Source Type: research
Bioinformatic Analysis of m6A Regulator-Mediated RNA Methylation Modification Patterns and Immune Microenvironment Characterization in Endometriosis
In conclusions, our integrated bioinformatics analysis results demonstrated that m6A-related genes METTL3, CBLL1 and YTHDF2 may be useful biomarkers for endometriosis in ectopic endometrium. The potential therapeutic approach of targeting m6A regulators holds promise for the treatment of endometriosis.PMID:38451401 | DOI:10.1007/s10528-024-10725-5 (Source: Biochemical Genetics)
Source: Biochemical Genetics - March 7, 2024 Category: Genetics & Stem Cells Authors: Weilin Zheng Zhiyi Fu Xi Tan Xuefang Liang Lixing Cao Source Type: research
