Involvement of Histone Acetyltransferase 1 (HAT1) in the Spermatogenesis of Non-Condensed Nuclear Sperm in Chinese Mitten Crab, Eriocheir sinensis
Biochem Genet. 2024 Feb 28. doi: 10.1007/s10528-024-10700-0. Online ahead of print.ABSTRACTChinese mitten crab, Eriocheir sinensis, is a decapod crustacean with a special, non-condensated nucleus in the sperm. Studies have shown that the nuclear compact state of male germ cells during the spermatogenesis is closely related to histone modification. To explore the possible role of histone acetyltransferase 1 (HAT1) in the chromatin organization during the E. sinensis spermatogenesis, we took the testis tissues of both adult and juvenile crabs as the materials of study and analyzed the biological functions of HAT1 by whole tr...
Source: Biochemical Genetics - February 28, 2024 Category: Genetics & Stem Cells Authors: Huiting Liu Yulian Tang Lishuang Sun Shu Li Lvjing Luo Zhengyu Chen Genliang Li Source Type: research
Identification and Validation of Lipid Metabolism Gene FASN-Associated miRNA in Wilms Tumor
This study intends to explore the role of miRNA in the lipid metabolism and development of Wilms tumor (WT) by detecting and analyzing the differences in the expression profiles of miRNAs between the tumor and adjacent normal tissue. Gene detection was performed in tumor tissues and adjacent normal tissues of three cases of WT to screen differentially expressed miRNAs (DEMs). According to our previous research, FASN, which participates in the lipid metabolism pathway, may be a target of WT. The starBase database was used to predict FASN-targeted miRNAs. The above two groups of miRNAs were intersected to obtain FASN-targete...
Source: Biochemical Genetics - February 28, 2024 Category: Genetics & Stem Cells Authors: Xiaoqing Wang Xiao Li Lin Tan Fengjun Zhang Jing Zhang Xu Zhao Yongfei Zhang Guoqiang Du Wei Liu Source Type: research
Involvement of Histone Acetyltransferase 1 (HAT1) in the Spermatogenesis of Non-Condensed Nuclear Sperm in Chinese Mitten Crab, Eriocheir sinensis
Biochem Genet. 2024 Feb 28. doi: 10.1007/s10528-024-10700-0. Online ahead of print.ABSTRACTChinese mitten crab, Eriocheir sinensis, is a decapod crustacean with a special, non-condensated nucleus in the sperm. Studies have shown that the nuclear compact state of male germ cells during the spermatogenesis is closely related to histone modification. To explore the possible role of histone acetyltransferase 1 (HAT1) in the chromatin organization during the E. sinensis spermatogenesis, we took the testis tissues of both adult and juvenile crabs as the materials of study and analyzed the biological functions of HAT1 by whole tr...
Source: Biochemical Genetics - February 28, 2024 Category: Genetics & Stem Cells Authors: Huiting Liu Yulian Tang Lishuang Sun Shu Li Lvjing Luo Zhengyu Chen Genliang Li Source Type: research
Cell Senescence-Related Genes as Biomarkers for Prognosis and Immunotherapeutic Response in Colon Cancer
This study has culminated in the development of a prognostic risk signature grounded in cell senescence-related genes for COAD. We posit that the CSS plays a regulatory role in immune infiltration, emerging as a robust biomarker for prognosis and a predictive indicator for immunotherapeutic responsiveness within the COAD landscape.PMID:38411939 | DOI:10.1007/s10528-024-10690-z (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Zhang Haibo Lan Tianyun Chen Xiaoman Han Xiaoyan Source Type: research
Comprehensive Analysis Based on the TCGA Database Identified SCIN as a Key DNA Methylation-Driver Gene in Epstein-Barr Virus-Associated Gastric Cancer
This study aims to analyze DNA methylation-driven genes (DMDG) in EBVaGC through bioinformatics methods, providing an important bioinformatics basis for the differential diagnosis and treatment of potential methylation biomarkers in EBVaGC. We downloaded the mRNA expression profiles and methylation datasets of EBVaGC and EBV-negative gastric cancer (EBVnGC) through the TCGA database to screen methylated-differentially expressed genes (MDEGs). DNA methylation-driver genes were identified based on MethylMix algorithm and key genes were further identified by LASSO regression and Random Forest algorithm. Then, we performed gen...
Source: Biochemical Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Zhiyuan Gong Chunxia Bi Wen Liu Bing Luo Source Type: research
Effects of Antimicrobial Peptides on Antioxidant Properties, Non-specific Immune Response and Gut Microbes of Tsinling Lenok Trout (Brachymystax lenok tsinlingensis)
In conclusion, AMP enhanced the antioxidant capacity, non-specific immunity, and intestinal health of tsinling lenok trout.PMID:38411941 | DOI:10.1007/s10528-024-10708-6 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Fang Ma Ruilin Ma Lei Zhao Source Type: research
In Silico Comparison of WRKY Transcription Factors in Wild and Cultivated Soybean and Their Co-expression Network Arbitrating Disease Resistance
Biochem Genet. 2024 Feb 27. doi: 10.1007/s10528-024-10701-z. Online ahead of print.ABSTRACTWRKY Transcription factors (TFs) play critical roles in plant defence mechanisms that are activated in response to biotic and abiotic stresses. However, information on the Glycine soja WRKYs (GsoWRKYs) is scarce. Owing to its importance in soybean breeding, here we identified putative WRKY TFs in wild soybean, and compared the results with Glycine max WRKYs (GmaWRKYs) by phylogenetic, conserved motif, and duplication analyses. Moreover, we explored the expression trends of WRKYs in G. max (oomycete, fungi, virus, bacteria, and soybea...
Source: Biochemical Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Muhammad Amjad Nawaz Hafiz Kashif Khalil Farrukh Azeem Muhammad Amjad Ali Igor Eduardovich Pamirsky Kirill S Golokhvast Seung Hwan Yang Rana Muhammad Atif Gyuhwa Chung Source Type: research
Circ_BLNK is a Unique Molecular Marker in Non-small Cell Lung Cancer
The objective of the present study was to functionally dissect the role and mechanism of circ_BLNK in NSCLC development and progression. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of circ_BLNK, miR-942-5p, and forkhead box protein O1 (FOXO1) in NSCLC tissues and cells. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay and colony formation assay detected cell proliferation; the protein expression levels were tested by western blot assay; cell apoptosis was measured by flow cytometry, and transwell assay ...
Source: Biochemical Genetics - February 27, 2024 Category: Genetics & Stem Cells Authors: Haihua Hong Dongxiao Ding Yonghua Zhang Yongbin Chen Shiyuan Chen Maofen Jiang Hairong Zhang Qinqin Wang Yue Hu Jianghong He Jiawei Yuan Source Type: research
Characterizing Homozygous Variants in Bardet-Biedl Syndrome-Associated Genes Within Iranian Families: Unveiling a Founder Variant in BBS2, c.471G & gt;A
Biochem Genet. 2024 Feb 26. doi: 10.1007/s10528-023-10637-w. Online ahead of print.ABSTRACTBardet-Biedl syndrome (BBS) is a rare inherited ciliopathy disorder characterized by a broad spectrum of clinical symptoms such as retinal dystrophy, obesity, polydactyly, genitourinary and kidney anomalies, learning disability, and hypogonadism. The understanding of the variants involved in BBS-causing genes remains incomplete, highlighting the need for further research to develop a molecular diagnostic strategy for this syndrome. Singleton whole-exome sequencing (WES) was performed on sixteen patients. Our study revealed (1) nine p...
Source: Biochemical Genetics - February 26, 2024 Category: Genetics & Stem Cells Authors: Masoumeh Heidari Feizabadi Masoome Alerasool Atieh Eslahi Emran Esmaeilzadeh Mohammad Yahya Vahidi Mehrjardi Mitra Saket Shima Farokhi Zohreh Fattahi Hamid Reza Khorram Khorshid Majid Mojarrad Source Type: research
Characterization of Complete Mitochondrial Genome of Badri Breed of Bos indicus (Bovidae: Bovinae): Selection Pressure and Comparative Analysis
In this study, we have generated the complete mitochondrial genome of the Badri breed of Bos indicus inhabiting a high-altitude environment to test the signatures of adaptive evolution on PCGs and their phylogenetic relationships. The complete mitogenome of the Badri breed is 16,339 bp and most tRNAs showed typical clover-leaf secondary structure with a few exceptions, like trnS1 and trnS2 without DHU arm and trnK without DHU loop. Comparative analysis of PCGs indicated that cox1 is the most conserved, while atp6 is the most variable gene. Moreover, the ratios of non-synonymous to synonymous substitution rates indicated th...
Source: Biochemical Genetics - February 26, 2024 Category: Genetics & Stem Cells Authors: Mansi Arya Abhishek Ghosh Kaomud Tyagi Inderjeet Tyagi Satpal Singh Bisht Vikas Kumar Source Type: research
Characterizing Homozygous Variants in Bardet-Biedl Syndrome-Associated Genes Within Iranian Families: Unveiling a Founder Variant in BBS2, c.471G & gt;A
Biochem Genet. 2024 Feb 26. doi: 10.1007/s10528-023-10637-w. Online ahead of print.ABSTRACTBardet-Biedl syndrome (BBS) is a rare inherited ciliopathy disorder characterized by a broad spectrum of clinical symptoms such as retinal dystrophy, obesity, polydactyly, genitourinary and kidney anomalies, learning disability, and hypogonadism. The understanding of the variants involved in BBS-causing genes remains incomplete, highlighting the need for further research to develop a molecular diagnostic strategy for this syndrome. Singleton whole-exome sequencing (WES) was performed on sixteen patients. Our study revealed (1) nine p...
Source: Biochemical Genetics - February 26, 2024 Category: Genetics & Stem Cells Authors: Masoumeh Heidari Feizabadi Masoome Alerasool Atieh Eslahi Emran Esmaeilzadeh Mohammad Yahya Vahidi Mehrjardi Mitra Saket Shima Farokhi Zohreh Fattahi Hamid Reza Khorram Khorshid Majid Mojarrad Source Type: research
Characterization of Complete Mitochondrial Genome of Badri Breed of Bos indicus (Bovidae: Bovinae): Selection Pressure and Comparative Analysis
In this study, we have generated the complete mitochondrial genome of the Badri breed of Bos indicus inhabiting a high-altitude environment to test the signatures of adaptive evolution on PCGs and their phylogenetic relationships. The complete mitogenome of the Badri breed is 16,339 bp and most tRNAs showed typical clover-leaf secondary structure with a few exceptions, like trnS1 and trnS2 without DHU arm and trnK without DHU loop. Comparative analysis of PCGs indicated that cox1 is the most conserved, while atp6 is the most variable gene. Moreover, the ratios of non-synonymous to synonymous substitution rates indicated th...
Source: Biochemical Genetics - February 26, 2024 Category: Genetics & Stem Cells Authors: Mansi Arya Abhishek Ghosh Kaomud Tyagi Inderjeet Tyagi Satpal Singh Bisht Vikas Kumar Source Type: research
Implication of m6A Methylation Regulators in the Immune Microenvironment of Bronchopulmonary Dysplasia
Biochem Genet. 2024 Feb 23. doi: 10.1007/s10528-024-10664-1. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) regulates gene expression and governs many important biological processes. However, the function of m6A in the development of bronchopulmonary dysplasia (BPD) remains poorly characterized. Thus, the purpose of this investigation was to evaluate the effects of m6A RNA methylation regulators on the development of BPD. BPD-related transcriptome data were downloaded from the GEO database. Differentially expressed m6A methylation regulators between BPD and control group were identified. Consensus clustering was co...
Source: Biochemical Genetics - February 23, 2024 Category: Genetics & Stem Cells Authors: Tianping Bao Haiyan Zhu Mengmeng Ma Tingting Sun Jingjing Hu JingYan Li Linxia Cao Huaiping Cheng Zhaofang Tian Source Type: research
Implication of m6A Methylation Regulators in the Immune Microenvironment of Bronchopulmonary Dysplasia
Biochem Genet. 2024 Feb 23. doi: 10.1007/s10528-024-10664-1. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) regulates gene expression and governs many important biological processes. However, the function of m6A in the development of bronchopulmonary dysplasia (BPD) remains poorly characterized. Thus, the purpose of this investigation was to evaluate the effects of m6A RNA methylation regulators on the development of BPD. BPD-related transcriptome data were downloaded from the GEO database. Differentially expressed m6A methylation regulators between BPD and control group were identified. Consensus clustering was co...
Source: Biochemical Genetics - February 23, 2024 Category: Genetics & Stem Cells Authors: Tianping Bao Haiyan Zhu Mengmeng Ma Tingting Sun Jingjing Hu JingYan Li Linxia Cao Huaiping Cheng Zhaofang Tian Source Type: research
Implication of m6A Methylation Regulators in the Immune Microenvironment of Bronchopulmonary Dysplasia
Biochem Genet. 2024 Feb 23. doi: 10.1007/s10528-024-10664-1. Online ahead of print.ABSTRACTN6-methyladenosine (m6A) regulates gene expression and governs many important biological processes. However, the function of m6A in the development of bronchopulmonary dysplasia (BPD) remains poorly characterized. Thus, the purpose of this investigation was to evaluate the effects of m6A RNA methylation regulators on the development of BPD. BPD-related transcriptome data were downloaded from the GEO database. Differentially expressed m6A methylation regulators between BPD and control group were identified. Consensus clustering was co...
Source: Biochemical Genetics - February 23, 2024 Category: Genetics & Stem Cells Authors: Tianping Bao Haiyan Zhu Mengmeng Ma Tingting Sun Jingjing Hu JingYan Li Linxia Cao Huaiping Cheng Zhaofang Tian Source Type: research
