siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Avian Diseases 67.1 Table of Contents
Avian Pathol. 2023 Aug;52(4):286-287. doi: 10.1080/03079457.2023.2235142.NO ABSTRACTPMID:37485675 | DOI:10.1080/03079457.2023.2235142 (Source: Avian Pathology)
Source: Avian Pathology - July 24, 2023 Category: Pathology Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Antigenic and molecular characterization of isolates of the Brazilian genotype BR-I (GI-11) of infectious bronchitis virus supports its recognition as BR-I serotype
Avian Pathol. 2023 Jul 21:1-16. doi: 10.1080/03079457.2023.2228725. Online ahead of print.ABSTRACTThe antigenic and molecular characteristics of BR-I infectious bronchitis viruses (IBVs) isolated from Brazil are reported. IBVs isolated from commercial flocks with different clinical manifestations between 2003 and 2019 were submitted to antigenic and molecular characterization. The complete S1 glycoprotein gene of 11 field isolates was amplified and sequenced. The virus neutralization (VN) test showed 94.75% neutralization with a BR-I isolate and 30% or less against other worldwide reference strains. The nucleotide and amin...
Source: Avian Pathology - July 21, 2023 Category: Pathology Authors: Jorge L Chac ón Ruy D Chac ón Christian J S ánchez-Llatas Jaime G Mor ín Claudete S Astolfi-Ferreira Antonio J Piantino Ferreira Source Type: research
Antigenic and molecular characterization of isolates of the Brazilian genotype BR-I (GI-11) of infectious bronchitis virus supports its recognition as BR-I serotype
Avian Pathol. 2023 Jul 21:1-16. doi: 10.1080/03079457.2023.2228725. Online ahead of print.ABSTRACTThe antigenic and molecular characteristics of BR-I infectious bronchitis viruses (IBVs) isolated from Brazil are reported. IBVs isolated from commercial flocks with different clinical manifestations between 2003 and 2019 were submitted to antigenic and molecular characterization. The complete S1 glycoprotein gene of 11 field isolates was amplified and sequenced. The virus neutralization (VN) test showed 94.75% neutralization with a BR-I isolate and 30% or less against other worldwide reference strains. The nucleotide and amin...
Source: Avian Pathology - July 21, 2023 Category: Pathology Authors: Jorge L Chac ón Ruy D Chac ón Christian J S ánchez-Llatas Jaime G Mor ín Claudete S Astolfi-Ferreira Antonio J Piantino Ferreira Source Type: research
Antigenic and molecular characterization of isolates of the Brazilian genotype BR-I (GI-11) of infectious bronchitis virus supports its recognition as BR-I serotype
Avian Pathol. 2023 Jul 21:1-16. doi: 10.1080/03079457.2023.2228725. Online ahead of print.ABSTRACTThe antigenic and molecular characteristics of BR-I infectious bronchitis viruses (IBVs) isolated from Brazil are reported. IBVs isolated from commercial flocks with different clinical manifestations between 2003 and 2019 were submitted to antigenic and molecular characterization. The complete S1 glycoprotein gene of 11 field isolates was amplified and sequenced. The virus neutralization (VN) test showed 94.75% neutralization with a BR-I isolate and 30% or less against other worldwide reference strains. The nucleotide and amin...
Source: Avian Pathology - July 21, 2023 Category: Pathology Authors: Jorge L Chac ón Ruy D Chac ón Christian J S ánchez-Llatas Jaime G Mor ín Claudete S Astolfi-Ferreira Antonio J Piantino Ferreira Source Type: research
Molecular characterization of the < em > HMTp210 < /em > gene of < em > Avibacterium paragallinarum < /em > and the proposition of a new genotyping method as alternative for classical serotyping
Avian Pathol. 2023 Jul 20:1-34. doi: 10.1080/03079457.2023.2239178. Online ahead of print.ABSTRACTAvibacterium paragallinarum (A. paragallinarum) is the etiological agent of infectious coryza (IC) in chickens and characterized by acute respiratory distress and severe drop in egg production. Vaccination is important in the control of IC outbreaks and the efficacy of vaccination is dependent on A. paragallinarum serovars included in the vaccine. Classical serotyping of A. paragallinarum is laborious and hampered by the poor availability of antigens and antisera. The hemagglutinin (HA), important in classical serotyping is en...
Source: Avian Pathology - July 20, 2023 Category: Pathology Authors: Rianne Buter Anneke Feberwee Sjaak de Wit Annet Heuvelink Ana da Silva Rodrigo Gallardo Edgardo Soriano Vargas Stefan Swanepoel Arne Jung Mathias T ödte Remco Dijkman Source Type: research
"Live IBD vaccine exacerbates disease and pathological effects of Asian lineage H9N2 LPAIV in chickens."
Avian Pathol. 2023 Jul 13:1-25. doi: 10.1080/03079457.2023.2236994. Online ahead of print.ABSTRACTAvian influenza H9N2 is one of the most circulating viruses in numerous Egyptian poultry farms. The Asian lineage H9N2 exhibits an immunosuppressive effect, and its pathogenicity is amplified when it co-infects with other pathogens, especially with the immunosuppressive infectious bursal disease virus (IBDV), resulting in increased mortality rates. Both vaccines and field infection can exacerbate the pathogenicity of H9N2, particularly in the bursa of Fabricius (B.F), causing more significant lymphoid depletion. To comprehend ...
Source: Avian Pathology - July 13, 2023 Category: Pathology Authors: N W Khalil M A Elshorbagy E M Elboraay A M Helal Source Type: research
