Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epi...
Source: Avian Pathology - August 15, 2023 Category: Pathology Authors: Xiangyu Huang Yiqin Cai Guihu Yin Zili Chen Jianing Hu Zichen Gao Xinyu Guo Fuqiang Xiong Xiuli Feng Source Type: research
Genetic Characterization of Avian Polyomaviruses Identified from Psittacine Birds in South Korea
This study improves our understanding of APV pathogenicity and provides a more detailed genetic characterization of APV strains.PMID:37565272 | DOI:10.1080/03079457.2023.2247347 (Source: Avian Pathology)
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: Ye-Ji Yun HyeSoon Song Yong-Kuk Kwon Choi-Kyu Park Hye-Ryoung Kim Source Type: research
Phylodynamic approaches to studying avian influenza virus
Avian Pathol. 2023 Aug 11:1-20. doi: 10.1080/03079457.2023.2236568. Online ahead of print.ABSTRACTAvian influenza viruses can cause severe disease in domestic and wild birds and are a pandemic threat. Phylodynamics is the study of how epidemiological, evolutionary, and immunological processes can interact to shape viral phylogenies. This review summarizes how phylodynamic methods have and could contribute to the study of avian influenza viruses. Specifically, we assess how phylodynamics can be used to examine viral spread within and between wild or domestic bird populations at various geographical scales, identify factors ...
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: L Carnegie J Raghwani G Fourni é S C Hill Source Type: research
Genetic Characterization of Avian Polyomaviruses Identified from Psittacine Birds in South Korea
This study improves our understanding of APV pathogenicity and provides a more detailed genetic characterization of APV strains.PMID:37565272 | DOI:10.1080/03079457.2023.2247347 (Source: Avian Pathology)
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: Ye-Ji Yun HyeSoon Song Yong-Kuk Kwon Choi-Kyu Park Hye-Ryoung Kim Source Type: research
Phylodynamic approaches to studying avian influenza virus
Avian Pathol. 2023 Aug 11:1-20. doi: 10.1080/03079457.2023.2236568. Online ahead of print.ABSTRACTAvian influenza viruses can cause severe disease in domestic and wild birds and are a pandemic threat. Phylodynamics is the study of how epidemiological, evolutionary, and immunological processes can interact to shape viral phylogenies. This review summarizes how phylodynamic methods have and could contribute to the study of avian influenza viruses. Specifically, we assess how phylodynamics can be used to examine viral spread within and between wild or domestic bird populations at various geographical scales, identify factors ...
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: L Carnegie J Raghwani G Fourni é S C Hill Source Type: research
Genetic Characterization of Avian Polyomaviruses Identified from Psittacine Birds in South Korea
This study improves our understanding of APV pathogenicity and provides a more detailed genetic characterization of APV strains.PMID:37565272 | DOI:10.1080/03079457.2023.2247347 (Source: Avian Pathology)
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: Ye-Ji Yun HyeSoon Song Yong-Kuk Kwon Choi-Kyu Park Hye-Ryoung Kim Source Type: research
Phylodynamic approaches to studying avian influenza virus
Avian Pathol. 2023 Aug 11:1-20. doi: 10.1080/03079457.2023.2236568. Online ahead of print.ABSTRACTAvian influenza viruses can cause severe disease in domestic and wild birds and are a pandemic threat. Phylodynamics is the study of how epidemiological, evolutionary, and immunological processes can interact to shape viral phylogenies. This review summarizes how phylodynamic methods have and could contribute to the study of avian influenza viruses. Specifically, we assess how phylodynamics can be used to examine viral spread within and between wild or domestic bird populations at various geographical scales, identify factors ...
Source: Avian Pathology - August 11, 2023 Category: Pathology Authors: L Carnegie J Raghwani G Fourni é S C Hill Source Type: research
Phylogenetic analysis of Newcastle disease virus detected in Eritrea between 2017 -2021
Avian Pathol. 2023 Aug 10:1-18. doi: 10.1080/03079457.2023.2247370. Online ahead of print.ABSTRACTAbstractThirty-five samples collected from chickens in 13 commercial farms in Eritrea between 2017 and 2021 following reports of disease were screened for Newcastle disease virus. Seventeen samples (50%) were shown to be positive by RT-PCR. An initial analysis of partial fusion (F) gene sequences of 10 representative samples indicated that the viruses belonged to subgenotype VII.1.1. Subsequent, full F gene sequence analysis of four of these representative samples confirmed the genotype of the viruses but also revealed that th...
Source: Avian Pathology - August 10, 2023 Category: Pathology Authors: Bereket Mihreteab Tebogo Kgotlele Fitsum Neguse Yodahi Petros Habtom Habtemariam Yordanos Berhane Munir Wehab Giovanni Cattoli Irene K Meki William G Dundon Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion
In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the a...
Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research
