Molecular assay for detecting MS-H vaccine strain and Immune response mechanisms in chickens receiving one or two doses of live MS-H vaccine
This study aimed to verify the PCR-RFLP molecular assay as a convenient technique for detecting the MS-H vaccine strain and to characterize the immune response mechanisms in experimental layer-type chickens receiving three different vaccination programs; a single dose at either 9 or 12 weeks of age or two doses at both 9 and 12 weeks of age. The PCR-RFLP assay, using restriction enzyme TasI to digest vlhA gene-targeted PCR amplicons, was performed to evaluate vaccine administration by detecting the MS-H vaccine strain in vaccinated chickens and differentiating it from non-vaccine strains as WVU1853 reference strain and Tha...
Source: Avian Pathology - October 4, 2023 Category: Pathology Authors: Kriengwich Limpavithayakul Nataya Charoenvisal Somsak Pakpinyo Source Type: research
Molecular assay for detecting MS-H vaccine strain and Immune response mechanisms in chickens receiving one or two doses of live MS-H vaccine
This study aimed to verify the PCR-RFLP molecular assay as a convenient technique for detecting the MS-H vaccine strain and to characterize the immune response mechanisms in experimental layer-type chickens receiving three different vaccination programs; a single dose at either 9 or 12 weeks of age or two doses at both 9 and 12 weeks of age. The PCR-RFLP assay, using restriction enzyme TasI to digest vlhA gene-targeted PCR amplicons, was performed to evaluate vaccine administration by detecting the MS-H vaccine strain in vaccinated chickens and differentiating it from non-vaccine strains as WVU1853 reference strain and Tha...
Source: Avian Pathology - October 4, 2023 Category: Pathology Authors: Kriengwich Limpavithayakul Nataya Charoenvisal Somsak Pakpinyo Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay
Avian Pathol. 2023 Sep 25:1-8. doi: 10.1080/03079457.2023.2254253. Online ahead of print.ABSTRACTThe widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achie...
Source: Avian Pathology - September 25, 2023 Category: Pathology Authors: Dongdong Yin Lei Yin Jieru Wang Yin Dai Xuehuai Shen Ruihong Zhao Kezong Qi Xiaocheng Pan Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand
Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recov...
Source: Avian Pathology - September 18, 2023 Category: Pathology Authors: Doan Hoang Phu Ruethai Narinthorn Nguyen Thi Nhung Niwat Chansiripornchai Patrick J Blackall Conny Turni Juan Carrique-Mas Thotsapol Thomrongsuwannakij Source Type: research
