siRNA targeting < em > Atp5a1 < /em > gene encoding ATPase α, the ligand of Peg fimbriae, reduced < em > Salmonella < /em > Enteritidis adhesion

In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesive protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 inLeghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the adhesion process.PMID:37526573 | DOI:10.1080/03079457.2023.2243842

Source: Avian Pathology - August 1, 2023 Category: Pathology Authors: Di Zhang Yi Jiang Yongyi Dong Lixia Fu Linlin Zhuang Kun Wu Xinhong Dou Bu Xu Chengming Wang Jiansen Gong Source Type: research