Applications of biaryl cyclization in the synthesis of cyclic enkephalin analogs with a highly restricted flexibility
Amino Acids. 2024 Mar 1;56(1):18. doi: 10.1007/s00726-023-03371-5.ABSTRACTA series of 10 cyclic, biaryl analogs of enkephalin, with Tyr or Phe residues at positions 1 and 4, were synthesized according to the Miyaura borylation and Suzuki coupling methodology. Biaryl bridges formed by side chains of the two aromatic amino acid residues are of the meta-meta, meta-para, para-meta, and para-para configuration. Conformational properties of the peptides were studied by CD and NMR. CD studies allowed only to compare conformations of individual peptides while NMR investigations followed by XPLOR calculations provided detailed info...
Source: Amino Acids - March 1, 2024 Category: Biochemistry Authors: Maria R óżanowska Gabriela Szczupaj Micha ł Nowakowski Priyadharshni Rajagopal Piotr F J Lipi ński Joanna Matali ńska Aleksandra Misicka Marek Lisowski Łukasz Jaremko Mariusz Jaremko Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
SNAT2-mediated regulation of estrogen and progesterone in the proliferation of goat mammary epithelial cells
In this study, we show that E2 and P4 could increase the proliferation of goat mammary epithelial cells (GMECs) and regulate SNAT2 mRNA and protein expression in a dose-dependent manner. Further investigation revealed that SNAT2 is abundantly expressed in the mammary gland during late pregnancy and early lactation, while knockdown and overexpression of SNAT2 in GMECs could inhibit or enhance E2- and P4-induced cell proliferation as well as mammalian target of rapamycin (mTOR) signaling. We also found that the accelerated proliferation induced by SNAT2 overexpression in GMECs was suppressed by the mTOR signaling pathway inh...
Source: Amino Acids - February 23, 2024 Category: Biochemistry Authors: Tingting Jiang Xiaoyue Ma Hanling Liu Qianqian Jia Jianguo Chen Yi Ding Ming Sun Hongmei Zhu Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
Hybrid 2D/3D-quantitative structure-activity relationship studies on the bioactivities and molecular mechanism of antibacterial peptides
Amino Acids. 2024 Feb 15;56(1):16. doi: 10.1007/s00726-024-03381-x.ABSTRACTAntimicrobial peptide (AMP) is the polypeptide, which protects the organism avoiding attack from pathogenic bacteria. Studies have shown that there were some antimicrobial peptides with molecular action mechanism involved in crossing the cell membrane without inducing severe membrane collapse, then interacting with cytoplasmic target-nucleic acid, and exerting antibacterial activity by interfacing the transmission of genetic information of pathogenic microorganisms. However, the relationship between the antibacterial activities and peptide structure...
Source: Amino Acids - February 15, 2024 Category: Biochemistry Authors: Qingguo Yan Fangfang Wang Bo Zhou Xiangna Lin Source Type: research
A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells
Amino Acids. 2024 Feb 13;56(1):15. doi: 10.1007/s00726-023-03361-7.ABSTRACTThe advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). In the present study, bioinformatics was used to screen the lncRNA-hidden polypeptides in LUAD. Analysis of protein expression was done by western blot or immunofluorescence assay. The functions of the polypeptide were determined by detecting its effects on cell viability, proliferation, migration, invasion, and...
Source: Amino Acids - February 13, 2024 Category: Biochemistry Authors: Xiaobing Han Liangxin Chen Peng Sun Xiuqing Wang Qian Zhao Lingfeng Liao Dejin Lou Nan Zhou Yujun Wang Source Type: research
