Nanomolar range of FAM237B can activate receptor GPR83
Amino Acids. 2023 Sep 9. doi: 10.1007/s00726-023-03328-8. Online ahead of print.ABSTRACTOur recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in viv...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Hao-Zheng Li Ya-Fen Wang Wen-Feng Hu Ya-Li Liu Zeng-Guang Xu Zhan-Yun Guo Source Type: research
Serum metabolomics study of narcolepsy type 1 based on ultra-performance liquid chromatography-tandem mass spectrometry
Amino Acids. 2023 Sep 10. doi: 10.1007/s00726-023-03315-z. Online ahead of print.ABSTRACTNarcolepsy is a chronic and underrecognized sleep disorder characterized by excessive daytime sleepiness and cataplexy. Furthermore, narcolepsy type 1 (NT1) has serious negative impacts on an individual's health, society, and the economy. Currently, many sleep centers lack the means to measure orexin levels in the cerebrospinal fluid. We aimed to analyze the characteristics of metabolite changes in patients with NT1, measured by ultra-performance liquid chromatography-tandem mass spectrometry. A principal component analysis (PCA), an o...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Qingqing Zhan Lili Wang Nan Liu Yuqing Yuan Liying Deng Yongmin Ding Fen Wang Jian Zhou Liang Xie Source Type: research
Nanomolar range of FAM237B can activate receptor GPR83
Amino Acids. 2023 Sep 9. doi: 10.1007/s00726-023-03328-8. Online ahead of print.ABSTRACTOur recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in viv...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Hao-Zheng Li Ya-Fen Wang Wen-Feng Hu Ya-Li Liu Zeng-Guang Xu Zhan-Yun Guo Source Type: research
Serum metabolomics study of narcolepsy type 1 based on ultra-performance liquid chromatography-tandem mass spectrometry
Amino Acids. 2023 Sep 10. doi: 10.1007/s00726-023-03315-z. Online ahead of print.ABSTRACTNarcolepsy is a chronic and underrecognized sleep disorder characterized by excessive daytime sleepiness and cataplexy. Furthermore, narcolepsy type 1 (NT1) has serious negative impacts on an individual's health, society, and the economy. Currently, many sleep centers lack the means to measure orexin levels in the cerebrospinal fluid. We aimed to analyze the characteristics of metabolite changes in patients with NT1, measured by ultra-performance liquid chromatography-tandem mass spectrometry. A principal component analysis (PCA), an o...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Qingqing Zhan Lili Wang Nan Liu Yuqing Yuan Liying Deng Yongmin Ding Fen Wang Jian Zhou Liang Xie Source Type: research
PepAnalyzer: predicting peptide properties using its sequence
Amino Acids. 2023 Sep 5. doi: 10.1007/s00726-023-03317-x. Online ahead of print.ABSTRACTPeptides are short linear molecules consisting of amino acids that play an essential role in most biological processes. They can treat diseases by working as a vaccine or antimicrobial agent and serves as a cancer molecule to deliver the drug to the target site for the treatment of cancer. They have the potential to solve the drawbacks of current medications and can be industrially produced in large quantities at low cost. However, poor chemical and physical stability, short circulating plasma half-life, and solubility are some issues t...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Vanshika Rustagi Shradheya R R Gupta Monika Bajaj Archana Singh Indrakant Kumar Singh Source Type: research
BamA-targeted antimicrobial peptide design for enhanced efficacy and reduced toxicity
This study employed computational simulation methods to screen and design AMPs specifically targeting ESKAPE pathogens. Particularly, AMPs were rationally designed to target the BamA and obtain novel antimicrobial peptide sequences. The designed AMPs were assessed for their antibacterial activities, mechanisms, and stability. Molecular docking and dynamics simulations demonstrated the interaction of both designed AMPs, 11pep and D-11pep, with the β1, β9, β15, and β16 chains of BamA, resulting in misfolding of outer membrane proteins and antibacterial effects. Subsequent antibacterial investigations confirmed the broad-...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Li Yang Minghe Luo Zhou Liu Yuepeng Li Zhihua Lin Shan Geng Yuanqiang Wang Source Type: research
PepAnalyzer: predicting peptide properties using its sequence
Amino Acids. 2023 Sep 5. doi: 10.1007/s00726-023-03317-x. Online ahead of print.ABSTRACTPeptides are short linear molecules consisting of amino acids that play an essential role in most biological processes. They can treat diseases by working as a vaccine or antimicrobial agent and serves as a cancer molecule to deliver the drug to the target site for the treatment of cancer. They have the potential to solve the drawbacks of current medications and can be industrially produced in large quantities at low cost. However, poor chemical and physical stability, short circulating plasma half-life, and solubility are some issues t...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Vanshika Rustagi Shradheya R R Gupta Monika Bajaj Archana Singh Indrakant Kumar Singh Source Type: research
BamA-targeted antimicrobial peptide design for enhanced efficacy and reduced toxicity
This study employed computational simulation methods to screen and design AMPs specifically targeting ESKAPE pathogens. Particularly, AMPs were rationally designed to target the BamA and obtain novel antimicrobial peptide sequences. The designed AMPs were assessed for their antibacterial activities, mechanisms, and stability. Molecular docking and dynamics simulations demonstrated the interaction of both designed AMPs, 11pep and D-11pep, with the β1, β9, β15, and β16 chains of BamA, resulting in misfolding of outer membrane proteins and antibacterial effects. Subsequent antibacterial investigations confirmed the broad-...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Li Yang Minghe Luo Zhou Liu Yuepeng Li Zhihua Lin Shan Geng Yuanqiang Wang Source Type: research
PepAnalyzer: predicting peptide properties using its sequence
Amino Acids. 2023 Sep 5. doi: 10.1007/s00726-023-03317-x. Online ahead of print.ABSTRACTPeptides are short linear molecules consisting of amino acids that play an essential role in most biological processes. They can treat diseases by working as a vaccine or antimicrobial agent and serves as a cancer molecule to deliver the drug to the target site for the treatment of cancer. They have the potential to solve the drawbacks of current medications and can be industrially produced in large quantities at low cost. However, poor chemical and physical stability, short circulating plasma half-life, and solubility are some issues t...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Vanshika Rustagi Shradheya R R Gupta Monika Bajaj Archana Singh Indrakant Kumar Singh Source Type: research
BamA-targeted antimicrobial peptide design for enhanced efficacy and reduced toxicity
This study employed computational simulation methods to screen and design AMPs specifically targeting ESKAPE pathogens. Particularly, AMPs were rationally designed to target the BamA and obtain novel antimicrobial peptide sequences. The designed AMPs were assessed for their antibacterial activities, mechanisms, and stability. Molecular docking and dynamics simulations demonstrated the interaction of both designed AMPs, 11pep and D-11pep, with the β1, β9, β15, and β16 chains of BamA, resulting in misfolding of outer membrane proteins and antibacterial effects. Subsequent antibacterial investigations confirmed the broad-...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Li Yang Minghe Luo Zhou Liu Yuepeng Li Zhihua Lin Shan Geng Yuanqiang Wang Source Type: research
PepAnalyzer: predicting peptide properties using its sequence
Amino Acids. 2023 Sep 5. doi: 10.1007/s00726-023-03317-x. Online ahead of print.ABSTRACTPeptides are short linear molecules consisting of amino acids that play an essential role in most biological processes. They can treat diseases by working as a vaccine or antimicrobial agent and serves as a cancer molecule to deliver the drug to the target site for the treatment of cancer. They have the potential to solve the drawbacks of current medications and can be industrially produced in large quantities at low cost. However, poor chemical and physical stability, short circulating plasma half-life, and solubility are some issues t...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Vanshika Rustagi Shradheya R R Gupta Monika Bajaj Archana Singh Indrakant Kumar Singh Source Type: research
BamA-targeted antimicrobial peptide design for enhanced efficacy and reduced toxicity
This study employed computational simulation methods to screen and design AMPs specifically targeting ESKAPE pathogens. Particularly, AMPs were rationally designed to target the BamA and obtain novel antimicrobial peptide sequences. The designed AMPs were assessed for their antibacterial activities, mechanisms, and stability. Molecular docking and dynamics simulations demonstrated the interaction of both designed AMPs, 11pep and D-11pep, with the β1, β9, β15, and β16 chains of BamA, resulting in misfolding of outer membrane proteins and antibacterial effects. Subsequent antibacterial investigations confirmed the broad-...
Source: Amino Acids - September 5, 2023 Category: Biochemistry Authors: Li Yang Minghe Luo Zhou Liu Yuepeng Li Zhihua Lin Shan Geng Yuanqiang Wang Source Type: research
Participation of protein metabolism in cancer progression and its potential targeting for the management of cancer
Amino Acids. 2023 Aug 30. doi: 10.1007/s00726-023-03316-y. Online ahead of print.ABSTRACTCancer malignancies may broadly be described as heterogeneous disorders manifested by uncontrolled cellular growth/division and proliferation. Tumor cells utilize metabolic reprogramming to accomplish the upregulated nutritional requirements for sustaining their uncontrolled growth, proliferation, and survival. Metabolic reprogramming also called altered or dysregulated metabolism undergoes modification in normal metabolic pathways for anabolic precursor's generation that serves to continue biomass formation that sustains the growth, p...
Source: Amino Acids - August 30, 2023 Category: Biochemistry Authors: Dalong Liu Yun Wang Xiaojiang Li Yan Wang Zhiqiang Zhang Zhifeng Wang Xudong Zhang Source Type: research
Developmental dynamics of homoarginine, ADMA and SDMA plasma levels from birth to adolescence
Amino Acids. 2023 Aug 30. doi: 10.1007/s00726-023-03318-w. Online ahead of print.ABSTRACTGuanidino compounds such as dimethylarginines (SDMA, ADMA) and L-homoarginine ((L-)hArg) can interfere with bioavailability and function of the main NO-donor L-arginine (L-Arg). High ADMA and SDMA but low L-hArg concentrations have been associated with cardio- and cerebrovascular events and mortality in adults. The role of guanidino compounds in paediatric patients remains less clear. We, therefore, compared guanidino compound levels in plasma samples of 57 individuals with chronic kidney disease (CKD) and 141 individuals without CKD f...
Source: Amino Acids - August 30, 2023 Category: Biochemistry Authors: Florence Baach Boglarka Meyer Jun Oh Susanne Lezius Rainer B öger Edzard Schwedhelm Chi-Un Choe Axel Neu Source Type: research
Participation of protein metabolism in cancer progression and its potential targeting for the management of cancer
Amino Acids. 2023 Aug 30. doi: 10.1007/s00726-023-03316-y. Online ahead of print.ABSTRACTCancer malignancies may broadly be described as heterogeneous disorders manifested by uncontrolled cellular growth/division and proliferation. Tumor cells utilize metabolic reprogramming to accomplish the upregulated nutritional requirements for sustaining their uncontrolled growth, proliferation, and survival. Metabolic reprogramming also called altered or dysregulated metabolism undergoes modification in normal metabolic pathways for anabolic precursor's generation that serves to continue biomass formation that sustains the growth, p...
Source: Amino Acids - August 30, 2023 Category: Biochemistry Authors: Dalong Liu Yun Wang Xiaojiang Li Yan Wang Zhiqiang Zhang Zhifeng Wang Xudong Zhang Source Type: research
