Untargeted metabolomics analysis of plasma metabolic characteristics in patients with acne and insulin resistance
This study aimed to identify significantly different metabolites and related metabolic pathways in the serum of acne vulgaris patients with or without insulin resistance. LC-MS/MS was used to analyze serum samples from patients about acne with insulin resistance (n = 51) and acne without insulin resistance (n = 69) to identify significant metabolites and metabolic pathways. In this study, 18 significant differential metabolites were screened for the first time. In the positive-ion mode, the upregulated substances were creatine, sarcosine, D-proline, uracil, Phe-Phe, L-pipecolic acid, and DL-phenylalanine; the downregulated...
Source: Amino Acids - September 19, 2023 Category: Biochemistry Authors: Qingqing He Huiling Shu Yu Peng Yang Xu Li Liu Jie Zhou Juan Zhao Xia Xiong Changqiang Li Source Type: research
Amino acid metabolism dysregulation associated with inflammation and insulin resistance in HIV-infected individuals with metabolic disorders
In this study, we performed targeted metabolomic analyses on 64 patients with HIV/AIDS and 21 healthy controls. Twenty-four amino acids and selected intermediate metabolites in the serum were quantitatively detected using high-performance liquid chromatography-tandem mass spectrometry, and characteristic changes and metabolic pathways were analyzed in HIV-infected patients with different degrees of abnormal glucose and lipid metabolism. Spearman's partial correlation was used to analyze the association between amino acids, biochemical parameters, and inflammatory cytokines. The results showed that the main metabolic pathwa...
Source: Amino Acids - September 19, 2023 Category: Biochemistry Authors: Jing Zhang Yanfang Chen Mingli Wang Liuting Zhong Linghua Li Zhongwen Yuan Shangrong Zou Source Type: research
Pancreastatin inhibitor PSTi8 ameliorates insulin resistance by decreasing fat accumulation and oxidative stress in high-fat diet-fed mice
Amino Acids. 2023 Sep 16. doi: 10.1007/s00726-023-03332-y. Online ahead of print.ABSTRACTAbnormal fat accumulation, enhanced free fatty acids (FFA) release, and their metabolites cause insulin resistance (IR) in major glucose-lipid metabolic organs such as skeletal muscle and adipose tissue. However, excessive lipolysis and FFA release from adipose tissue elevate plasma FFA levels leading to oxidative stress and skeletal muscle IR. Indeed, in obese individuals, there is enhanced pro-inflammatory secretion from adipose tissue influencing insulin signaling in skeletal muscles. Here, we investigated the effect of PSTi8 on FFA...
Source: Amino Acids - September 16, 2023 Category: Biochemistry Authors: Richa Garg Arun Agarwal Roshan Katekar Umesh Kumar Goand Naveen Singh Shubhi Yadav Shivam Rathaur Saurabh Verma Debalina Maity Sachin Vishwakarma Jiaur R Gayen Source Type: research
Pancreastatin inhibitor PSTi8 ameliorates insulin resistance by decreasing fat accumulation and oxidative stress in high-fat diet-fed mice
Amino Acids. 2023 Sep 16. doi: 10.1007/s00726-023-03332-y. Online ahead of print.ABSTRACTAbnormal fat accumulation, enhanced free fatty acids (FFA) release, and their metabolites cause insulin resistance (IR) in major glucose-lipid metabolic organs such as skeletal muscle and adipose tissue. However, excessive lipolysis and FFA release from adipose tissue elevate plasma FFA levels leading to oxidative stress and skeletal muscle IR. Indeed, in obese individuals, there is enhanced pro-inflammatory secretion from adipose tissue influencing insulin signaling in skeletal muscles. Here, we investigated the effect of PSTi8 on FFA...
Source: Amino Acids - September 16, 2023 Category: Biochemistry Authors: Richa Garg Arun Agarwal Roshan Katekar Umesh Kumar Goand Naveen Singh Shubhi Yadav Shivam Rathaur Saurabh Verma Debalina Maity Sachin Vishwakarma Jiaur R Gayen Source Type: research
Pancreastatin inhibitor PSTi8 ameliorates insulin resistance by decreasing fat accumulation and oxidative stress in high-fat diet-fed mice
Amino Acids. 2023 Sep 16. doi: 10.1007/s00726-023-03332-y. Online ahead of print.ABSTRACTAbnormal fat accumulation, enhanced free fatty acids (FFA) release, and their metabolites cause insulin resistance (IR) in major glucose-lipid metabolic organs such as skeletal muscle and adipose tissue. However, excessive lipolysis and FFA release from adipose tissue elevate plasma FFA levels leading to oxidative stress and skeletal muscle IR. Indeed, in obese individuals, there is enhanced pro-inflammatory secretion from adipose tissue influencing insulin signaling in skeletal muscles. Here, we investigated the effect of PSTi8 on FFA...
Source: Amino Acids - September 16, 2023 Category: Biochemistry Authors: Richa Garg Arun Agarwal Roshan Katekar Umesh Kumar Goand Naveen Singh Shubhi Yadav Shivam Rathaur Saurabh Verma Debalina Maity Sachin Vishwakarma Jiaur R Gayen Source Type: research
Using the local symmetry in amino acids sequences of polypeptides to improve the predictive potential of models of their inhibitor activity
Amino Acids. 2023 Sep 14. doi: 10.1007/s00726-023-03322-0. Online ahead of print.ABSTRACTThe minimal inhibitory concentrations (pMIC) are a valuable measure of the biological activity of polypeptides. Numerical data on the pMIC are necessary to systematize knowledge on polypeptides' biochemical behaviour. The model of negative decimal logarithm of pMIC of polypeptides in the form of a mathematical function of a sequence of amino acids is suggested. The suggested model is based on the so-called correlation weights of amino acids together with the correlation weights of fragments of local symmetry (FLS). Three kinds of the F...
Source: Amino Acids - September 14, 2023 Category: Biochemistry Authors: Alla P Toropova Andrey A Toropov Source Type: research
Using the local symmetry in amino acids sequences of polypeptides to improve the predictive potential of models of their inhibitor activity
Amino Acids. 2023 Sep 14. doi: 10.1007/s00726-023-03322-0. Online ahead of print.ABSTRACTThe minimal inhibitory concentrations (pMIC) are a valuable measure of the biological activity of polypeptides. Numerical data on the pMIC are necessary to systematize knowledge on polypeptides' biochemical behaviour. The model of negative decimal logarithm of pMIC of polypeptides in the form of a mathematical function of a sequence of amino acids is suggested. The suggested model is based on the so-called correlation weights of amino acids together with the correlation weights of fragments of local symmetry (FLS). Three kinds of the F...
Source: Amino Acids - September 14, 2023 Category: Biochemistry Authors: Alla P Toropova Andrey A Toropov Source Type: research
Using the local symmetry in amino acids sequences of polypeptides to improve the predictive potential of models of their inhibitor activity
Amino Acids. 2023 Sep 14. doi: 10.1007/s00726-023-03322-0. Online ahead of print.ABSTRACTThe minimal inhibitory concentrations (pMIC) are a valuable measure of the biological activity of polypeptides. Numerical data on the pMIC are necessary to systematize knowledge on polypeptides' biochemical behaviour. The model of negative decimal logarithm of pMIC of polypeptides in the form of a mathematical function of a sequence of amino acids is suggested. The suggested model is based on the so-called correlation weights of amino acids together with the correlation weights of fragments of local symmetry (FLS). Three kinds of the F...
Source: Amino Acids - September 14, 2023 Category: Biochemistry Authors: Alla P Toropova Andrey A Toropov Source Type: research
PyProtif: a PyMol plugin to retrieve and visualize protein motifs for structural studies
Amino Acids. 2023 Sep 12. doi: 10.1007/s00726-023-03323-z. Online ahead of print.ABSTRACTProteins often possess several motifs and the ones with similar motifs were found to have similar biochemical properties and thus related biological functions. Thereby, multiple databases were developed to store information on such motifs in proteins. For instance, PDBsum stores the results of Promotif's generated structural motifs and Pfam stores pre-computed patterns of functional domains. In addition to the fact that all this stored information is extremely useful, we can further augment its importance if we ought to integrate these...
Source: Amino Acids - September 12, 2023 Category: Biochemistry Authors: Gilbert El Khoury Wael Azzam Joseph Rebehmed Source Type: research
PyProtif: a PyMol plugin to retrieve and visualize protein motifs for structural studies
Amino Acids. 2023 Sep 12. doi: 10.1007/s00726-023-03323-z. Online ahead of print.ABSTRACTProteins often possess several motifs and the ones with similar motifs were found to have similar biochemical properties and thus related biological functions. Thereby, multiple databases were developed to store information on such motifs in proteins. For instance, PDBsum stores the results of Promotif's generated structural motifs and Pfam stores pre-computed patterns of functional domains. In addition to the fact that all this stored information is extremely useful, we can further augment its importance if we ought to integrate these...
Source: Amino Acids - September 12, 2023 Category: Biochemistry Authors: Gilbert El Khoury Wael Azzam Joseph Rebehmed Source Type: research
PDZK1 improves ventricular remodeling in hypertensive rats by regulating the stability of the Mas receptor
This study provides a scientific basis for the prevention and treatment of ventricular remodeling.PMID:37696999 | DOI:10.1007/s00726-023-03331-z (Source: Amino Acids)
Source: Amino Acids - September 11, 2023 Category: Biochemistry Authors: Jinyu Chi Wanlin Li Yang Xu Xiuzhi Li Xiaohui Zhang Zhiyu Shi Chunnan Liu Wenxiu Liu Meng Zhao Yan Meng Dechao Zhao Source Type: research
PDZK1 improves ventricular remodeling in hypertensive rats by regulating the stability of the Mas receptor
This study provides a scientific basis for the prevention and treatment of ventricular remodeling.PMID:37696999 | DOI:10.1007/s00726-023-03331-z (Source: Amino Acids)
Source: Amino Acids - September 11, 2023 Category: Biochemistry Authors: Jinyu Chi Wanlin Li Yang Xu Xiuzhi Li Xiaohui Zhang Zhiyu Shi Chunnan Liu Wenxiu Liu Meng Zhao Yan Meng Dechao Zhao Source Type: research
Nanomolar range of FAM237B can activate receptor GPR83
Amino Acids. 2023 Sep 9. doi: 10.1007/s00726-023-03328-8. Online ahead of print.ABSTRACTOur recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in viv...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Hao-Zheng Li Ya-Fen Wang Wen-Feng Hu Ya-Li Liu Zeng-Guang Xu Zhan-Yun Guo Source Type: research
Serum metabolomics study of narcolepsy type 1 based on ultra-performance liquid chromatography-tandem mass spectrometry
Amino Acids. 2023 Sep 10. doi: 10.1007/s00726-023-03315-z. Online ahead of print.ABSTRACTNarcolepsy is a chronic and underrecognized sleep disorder characterized by excessive daytime sleepiness and cataplexy. Furthermore, narcolepsy type 1 (NT1) has serious negative impacts on an individual's health, society, and the economy. Currently, many sleep centers lack the means to measure orexin levels in the cerebrospinal fluid. We aimed to analyze the characteristics of metabolite changes in patients with NT1, measured by ultra-performance liquid chromatography-tandem mass spectrometry. A principal component analysis (PCA), an o...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Qingqing Zhan Lili Wang Nan Liu Yuqing Yuan Liying Deng Yongmin Ding Fen Wang Jian Zhou Liang Xie Source Type: research
Nanomolar range of FAM237B can activate receptor GPR83
Amino Acids. 2023 Sep 9. doi: 10.1007/s00726-023-03328-8. Online ahead of print.ABSTRACTOur recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in viv...
Source: Amino Acids - September 9, 2023 Category: Biochemistry Authors: Hao-Zheng Li Ya-Fen Wang Wen-Feng Hu Ya-Li Liu Zeng-Guang Xu Zhan-Yun Guo Source Type: research
