Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid
Amino Acids. 2023 Nov 16. doi: 10.1007/s00726-023-03348-4. Online ahead of print.ABSTRACTThe conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPL...
Source: Amino Acids - November 16, 2023 Category: Biochemistry Authors: Shakti K Maurya Abhishek Tripathi Selvakumar Karuthapandi Harkesh B Singh Source Type: research
A novel antibacterial approach of Cecropin-B peptide loaded on chitosan nanoparticles against MDR Klebsiella pneumoniae isolates
This study aims to encapsulate the cationic antimicrobial peptide; Cecropin-B (Cec-B), to increase its lifetime, drug targeting, and efficacy and study the antimicrobial effect of free and encapsulated recombinant rCec-B peptide on multidrug-resistant K. pneumoniae (MDR-KP) isolates. Fifty isolates were collected from different clinical departments at Theodore Bilharz Research Institute. Minimal inhibitory concentrations (MICs) of rCec-B against MDR-KP isolates were determined by the broth microdilution test. In addition, encapsulation of rCec-B peptide into chitosan nanoparticles and studying its bactericidal effect again...
Source: Amino Acids - November 15, 2023 Category: Biochemistry Authors: Hend Okasha Heba Dahroug Abdullah E Gouda Mohamed Abbas Shemis Source Type: research
Amino acid-stimulated insulin secretion: a path forward in type 2 diabetes
Amino Acids. 2023 Nov 15. doi: 10.1007/s00726-023-03352-8. Online ahead of print.ABSTRACTQualitative and quantitatively appropriate insulin secretion is essential for optimal control of blood glucose. Beta-cells of the pancreas produce and secrete insulin in response to glucose and non-glucose stimuli including amino acids. In this manuscript, we review the literature on amino acid-stimulated insulin secretion in oral and intravenous in vivo studies, in addition to the in vitro literature, and describe areas of consensus and gaps in understanding. We find promising evidence that the synergism of amino acid-stimulated insul...
Source: Amino Acids - November 15, 2023 Category: Biochemistry Authors: Jelena Kolic WenQing Grace Sun James D Johnson Nicola Guess Source Type: research
A novel antibacterial approach of Cecropin-B peptide loaded on chitosan nanoparticles against MDR Klebsiella pneumoniae isolates
This study aims to encapsulate the cationic antimicrobial peptide; Cecropin-B (Cec-B), to increase its lifetime, drug targeting, and efficacy and study the antimicrobial effect of free and encapsulated recombinant rCec-B peptide on multidrug-resistant K. pneumoniae (MDR-KP) isolates. Fifty isolates were collected from different clinical departments at Theodore Bilharz Research Institute. Minimal inhibitory concentrations (MICs) of rCec-B against MDR-KP isolates were determined by the broth microdilution test. In addition, encapsulation of rCec-B peptide into chitosan nanoparticles and studying its bactericidal effect again...
Source: Amino Acids - November 15, 2023 Category: Biochemistry Authors: Hend Okasha Heba Dahroug Abdullah E Gouda Mohamed Abbas Shemis Source Type: research
Amino acid-stimulated insulin secretion: a path forward in type 2 diabetes
Amino Acids. 2023 Nov 15. doi: 10.1007/s00726-023-03352-8. Online ahead of print.ABSTRACTQualitative and quantitatively appropriate insulin secretion is essential for optimal control of blood glucose. Beta-cells of the pancreas produce and secrete insulin in response to glucose and non-glucose stimuli including amino acids. In this manuscript, we review the literature on amino acid-stimulated insulin secretion in oral and intravenous in vivo studies, in addition to the in vitro literature, and describe areas of consensus and gaps in understanding. We find promising evidence that the synergism of amino acid-stimulated insul...
Source: Amino Acids - November 15, 2023 Category: Biochemistry Authors: Jelena Kolic WenQing Grace Sun James D Johnson Nicola Guess Source Type: research
Safety assessment of L-Arg oral intake in healthy subjects: a systematic review of randomized control trials
Amino Acids. 2023 Nov 10. doi: 10.1007/s00726-023-03354-6. Online ahead of print.ABSTRACTL-Arg is a nonessential amino acid but has many physiological roles. Accordingly, L-Arg has been used in various fields, but there is only limited information available about its safety upon overdose. Generally, the no-observed adverse effect level (NOAEL) is used when setting the upper amount for chemical substances. Recently, systematic reviews have been used to assess the safety as well as the effectiveness and usefulness of them. Therefore, we conducted an assessment of the safety of the oral intake of L-Arg in healthy subjects usi...
Source: Amino Acids - November 10, 2023 Category: Biochemistry Authors: Yui Kuramochi Mai Murata Akihide Sumino Hideko Sone Kohsuke Hayamizu Source Type: research
Safety assessment of L-Arg oral intake in healthy subjects: a systematic review of randomized control trials
Amino Acids. 2023 Nov 10. doi: 10.1007/s00726-023-03354-6. Online ahead of print.ABSTRACTL-Arg is a nonessential amino acid but has many physiological roles. Accordingly, L-Arg has been used in various fields, but there is only limited information available about its safety upon overdose. Generally, the no-observed adverse effect level (NOAEL) is used when setting the upper amount for chemical substances. Recently, systematic reviews have been used to assess the safety as well as the effectiveness and usefulness of them. Therefore, we conducted an assessment of the safety of the oral intake of L-Arg in healthy subjects usi...
Source: Amino Acids - November 10, 2023 Category: Biochemistry Authors: Yui Kuramochi Mai Murata Akihide Sumino Hideko Sone Kohsuke Hayamizu Source Type: research
Safety assessment of L-Arg oral intake in healthy subjects: a systematic review of randomized control trials
Amino Acids. 2023 Nov 10. doi: 10.1007/s00726-023-03354-6. Online ahead of print.ABSTRACTL-Arg is a nonessential amino acid but has many physiological roles. Accordingly, L-Arg has been used in various fields, but there is only limited information available about its safety upon overdose. Generally, the no-observed adverse effect level (NOAEL) is used when setting the upper amount for chemical substances. Recently, systematic reviews have been used to assess the safety as well as the effectiveness and usefulness of them. Therefore, we conducted an assessment of the safety of the oral intake of L-Arg in healthy subjects usi...
Source: Amino Acids - November 10, 2023 Category: Biochemistry Authors: Yui Kuramochi Mai Murata Akihide Sumino Hideko Sone Kohsuke Hayamizu Source Type: research
