Identification of adulteration in botanical samples with untargeted metabolomics.
Abstract
Adulteration remains an issue in the dietary supplement industry, including botanical supplements. While it is common to employ a targeted analysis to detect known adulterants, this is difficult when little is known about the sample set. With this study, untargeted metabolomics using liquid chromatography coupled to ultraviolet-visible spectroscopy (LC-UV) or high-resolution mass spectrometry (LC-MS) was employed to detect adulteration in botanical dietary supplements. A training set was prepared by combining Hydrastis canadensis L. with a known adulterant, Coptis chinensis Franch., in ratios rang...
Source: Analytical and Bioanalytical Chemistry - April 28, 2020 Category: Chemistry Authors: Wallace ED, Todd DA, Harnly JM, Cech NB, Kellogg JJ Tags: Anal Bioanal Chem Source Type: research
Progress and prospects in the management of bacterial infections and developments in Phytotherapeutic modalities.
Abstract
The advent of antibiotics revolutionized the medical care resulting in significantly reduced mortality and morbidity caused by infectious diseases. However, excessive use of antibiotics has led to the development of antibiotic resistance and indeed, the incidence of multi-drug resistant pathogens is considered as a major disadvantage in the medication strategy, which led the scholar's attention toward innovative antibiotic sources in recent years. Medicinal plants contained a variety of secondary metabolites with a wide range of therapeutic potential against the resistant microbes. Therefore, the ...
Source: Clinical and Experimental Pharmacology and Physiology - February 15, 2020 Category: Drugs & Pharmacology Authors: Akram M, Riaz M, Munir N, Rasul A, Daniyal M, Ali Shah SM, Shariati MA, Shaheen G, Akhtar N, Parveen F, Akhter N, Owais Ghauri A, Chishti AW, Usman Sarwar M, Said Khan F Tags: Clin Exp Pharmacol Physiol Source Type: research
Composite score analysis for unsupervised comparison and network visualization of metabolomics data.
Abstract
Metabolomics-based approaches are becoming increasingly popular to interrogate the chemical basis for phenotypic differences in biological systems. Successful metabolomics studies employ multivariate data analysis to compare large and highly complex datasets. A primary tool for unsupervised statistical analyses, principal component analysis (PCA), relies on the selection of a subsection of a maximum of three components from a larger model to visually represent similarity. The use of only three principal components limits the comprehensiveness of the model and can mask discrimination between sample...
Source: Analytica Chimica Acta - December 24, 2019 Category: Chemistry Authors: Kellogg JJ, Kvalheim OM, Cech NB Tags: Anal Chim Acta Source Type: research
Botanical metabolite ions extraction from full electrospray ionization mass spectrometry using high-dimensional penalized regression
ConclusionThe multivariate analytical workflow selects “true” metabolite ions signals and provides an alternative approach to a univariatep-value cutoff fromt-test, thus enhancing the data analysis process of metabolomics. (Source: Metabolomics)
Source: Metabolomics - October 3, 2019 Category: Biology Source Type: research
Quantification of natural products in herbal supplements: A combined NMR approach applied on goldenseal
Publication date: 20 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 165Author(s): Phuong Mai Le, Clément Milande, Estelle Martineau, Patrick Giraudeau, Jonathan FarjonAbstractAuthentication of natural products is of major relevance in the context of manufactured drugs or herbal supplements since such active products generate a lucrative market. The analytical method to identify and quantify valuable natural products is critical for quality control and product assignment of herbal supplements. In this framework, we propose to apply a recently developed quantitative 2D NMR approach called Q Q...
Source: Journal of Pharmaceutical and Biomedical Analysis - December 11, 2018 Category: Drugs & Pharmacology Source Type: research
Detection of adulteration in Hydrastis canadensis (goldenseal) dietary supplements via untargeted mass spectrometry-based metabolomics.
Abstract
Current estimates report that approximately 25% of U.S. adults use dietary supplements for medicinal purposes. Yet, regulation and transparency within the dietary supplement industry remains a challenge, and economic incentives encourage adulteration or augmentation of botanical dietary supplement products. Undisclosed changes to the dietary supplement composition could impact safety and efficacy; thus, there is a continued need to monitor possible botanical adulteration or mis-identification. Goldenseal, Hydrastis canadensis L. (Ranunculaceae), is a well-known botanical used to combat bacterial i...
Source: Food and Chemical Toxicology - July 18, 2018 Category: Food Science Authors: Wallace ED, Oberlies NH, Cech NB, Kellogg JJ Tags: Food Chem Toxicol Source Type: research
Potential pharmacokinetic herb-drug interactions: have we overlooked the importance of human carboxylesterases 1 and 2?
Abstract
Herbal products have grown steadily across the globe over the past 10 years and have increasingly been incorporated into western medicine for healthcare aims, thereby causing potential pharmacokinetic herb-drug interactions (HDI) through the inhibition or induction of drug-metabolizing enzymes and transporters. Human carboxylesterases 1 (CES1) and 2 (CES2) are versatile enzymes involvement in the metabolism of endogenous and exogenous compounds including many important therapeutic medications. Moreover, the growing number of CES substrate drugs also underscores the importance of CES1 and CES2. Her...
Source: Current Drug Metabolism - March 30, 2018 Category: Drugs & Pharmacology Authors: Xu J, Qiu JC, Ji X, Guo HL, Wang X, Zhang B, Wang T, Chen F Tags: Curr Drug Metab Source Type: research
FT-NIR characterization with chemometric analyses to differentiate goldenseal from common adulterants.
This study demonstrates that NIR spectroscopy coupled with chemometric analyses is a good tool for industry and investigators to implement for rapid detection of goldenseal adulteration in the marketplace, but also indicates that the specific approach to chemometric analysis must be evaluated and selected on a case-by-case basis in order to achieve useful sensitivity and specificity.
PMID: 29421241 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - February 5, 2018 Category: Biochemistry Authors: Liu Y, Finley J, Betz JM, Brown PN Tags: Fitoterapia Source Type: research
Biochemometrics to Identify Synergists and Additives
from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)
Journal of Natural ProductsDOI: 10.1021/acs.jnatprod.7b00654 (Source: Journal of Natural Products)
Source: Journal of Natural Products - November 1, 2017 Category: Biochemistry Authors: Emily R. Britton, Joshua J. Kellogg, Olav M. Kvalheim and Nadja B. Cech Source Type: research
Molecules, Vol. 22, Pages 1847: Berberine Activates Aryl Hydrocarbon Receptor but Suppresses CYP1A1 Induction through miR-21-3p Stimulation in MCF-7 Breast Cancer Cells
This study aimed to illustrate the effects of protoberberines on aryl hydrocarbon receptor (AhR) activation and cytochrome P450 (CYP) 1 in the estrogen receptor (ER)α(+) MCF-7 breast cancer cells. Among protoberberines at non-cytotoxic concentrations (≤10 μM), berberine had the most potent and statistically significant effects on AhR activation and CYP1A1/1A2/1B1 mRNA induction. The 24-h exposure to 10 μM berberine did not change CYP1A1 mRNA stability, protein level and function. Berberine significantly increased micro RNA (miR)-21-3p by 36% and the transfection of an inhibitor of miR-21-3p restored the induction of C...
Source: Molecules - October 28, 2017 Category: Chemistry Authors: Sheng-Nan Lo Chun-Wei Wang Yueh-Shieh Chen Chiung-Chiao Huang Tian-Shung Wu Lih-Ann Li I-Jung Lee Yune-Fang Ueng Tags: Article Source Type: research
Common Herbal Dietary Supplement-Drug Interactions.
Abstract
Nearly 25% of U.S. adults report concurrently taking a prescription medication with a dietary supplement. Some supplements, such as St. John's wort and goldenseal, are known to cause clinically important drug interactions and should be avoided by most patients receiving any pharmacologic therapy. However, many other supplements are predicted to cause interactions based only on in vitro studies that have not been confirmed or have been refuted in human clinical trials. Some supplements may cause interactions with a few medications but are likely to be safe with other medications (e.g., curcumin, ec...
Source: American Family Physician - July 15, 2017 Category: Primary Care Authors: Asher GN, Corbett AH, Hawke RL Tags: Am Fam Physician Source Type: research
Secondary metabolites from the leaves of the medicinal plant goldenseal (Hydrastis canadensis)
Publication date: June 2017 Source:Phytochemistry Letters, Volume 20 Author(s): Martha Leyte-Lugo, Emily R. Britton, Daniel H. Foil, Adam R. Brown, Daniel A. Todd, José Rivera-Chávez, Nicholas H. Oberlies, Nadja B. Cech The study presented herein constitutes an extensive investigation of constituents in Hydrastis canadensis L. (Ranunculaceae) leaves. It describes the isolation and identification of two previously unknown compounds, 3,4-dimethoxy-2-(methoxycarbonyl)benzoic acid (1) and 3,5,3′-trihydroxy-7,4′-dimethoxy-6,8-C-dimethyl-flavone (2), along with the known compounds (±)-chilenine (3), (2R)-5,4′-...
Source: Phytochemistry Letters - May 3, 2017 Category: Chemistry Source Type: research
New strategies to identify synergists from Hydrastis canadensis (goldenseal)
Planta Med 2016; 81: S1-S381 DOI: 10.1055/s-0036-1596182 Georg Thieme Verlag KG Stuttgart · New YorkArticle in Thieme eJournals: Table of contents | Full text (Source: Planta Medica)
Source: Planta Medica - December 13, 2016 Category: Drugs & Pharmacology Authors: Britton, ER Kellogg, JJ Kvalheim, OM Cech, NB Tags: Abstracts Source Type: research
Berberine and Its Role in Chronic Disease.
Authors: Cicero AF, Baggioni A
Abstract
Berberine is a quaternary ammonium salt from the protoberberine group of isoquinoline alkaloids. It is found in such plants as Berberis [e.g. Berberis aquifolium (Oregon grape), Berberis vulgaris (barberry), Berberis aristata (tree turmeric)], Hydrastis canadensis (goldenseal), Xanthorhiza simplicissima (yellowroot), Phellodendron amurense ([2]) (Amur corktree), Coptis chinensis (Chinese goldthread), Tinospora cordifolia, Argemone mexicana (prickly poppy) and Eschscholzia californica (Californian poppy). In vitro it exerts significant anti-inflammatory and antioxidan...
Source: Advances in Experimental Medicine and Biology - September 29, 2016 Category: Research Tags: Adv Exp Med Biol Source Type: research
Antimicrobial fungal endophytes from the botanical medicine goldenseal (Hydrastis canadensis)
Publication date: September 2016 Source:Phytochemistry Letters, Volume 17 Author(s): Joseph M. Egan, Amninder Kaur, Huzefa A. Raja, Joshua J. Kellogg, Nicholas H. Oberlies, Nadja B. Cech The potential of fungal endophytes to alter or contribute to plant chemistry and biology has been the topic of a great deal of recent interest. For plants that are used medicinally, it has been proposed that endophytes might play an important role in biological activity. With this study, we sought to identify antimicrobial fungal endophytes from the medicinal plant goldenseal (Hydrastis canadensis L., Ranunculaceae), a plant used i...
Source: Phytochemistry Letters - August 7, 2016 Category: Chemistry Source Type: research
