Evaluation of DNA extraction methods for the detection of Shiga toxin producing Escherichia coli in food by polymerase chain reaction
In this study, three DNA extraction protocols using commercial kits (Instagene Matrix with Beckman Coulter Ampure XP Beads; Qiagen Gentra Puregene Yeast/Bact. Kit; Qiagen DNeasy Blood & Tissue) were assessed as alternative DNA extraction methods for the detection of the Shiga toxin gene by PCR in enrichments from sixteen different foods inoculated with STEC O157. The inoculated foods were bean sprouts, blackberries, blue cheese, cilantro, cocoa powder, coleslaw, cream of mushroom dried soup mix, cream of vegetable dried soup mix, flaxseed, guacamole, peanut butter, soft cheese, soy butter, spinach, walnut, and wheat fl...
Source: International Journal of Food Microbiology - July 20, 2023 Category: Food Science Authors: Tanis McMahon Mariam Abdelmesih Alexander Gill Source Type: research
Evaluation of DNA extraction methods for the detection of Shiga toxin producing Escherichia coli in food by polymerase chain reaction
In this study, three DNA extraction protocols using commercial kits (Instagene Matrix with Beckman Coulter Ampure XP Beads; Qiagen Gentra Puregene Yeast/Bact. Kit; Qiagen DNeasy Blood & Tissue) were assessed as alternative DNA extraction methods for the detection of the Shiga toxin gene by PCR in enrichments from sixteen different foods inoculated with STEC O157. The inoculated foods were bean sprouts, blackberries, blue cheese, cilantro, cocoa powder, coleslaw, cream of mushroom dried soup mix, cream of vegetable dried soup mix, flaxseed, guacamole, peanut butter, soft cheese, soy butter, spinach, walnut, and wheat fl...
Source: International Journal of Food Microbiology - July 20, 2023 Category: Food Science Authors: Tanis McMahon Mariam Abdelmesih Alexander Gill Source Type: research
Evaluation of DNA extraction methods for the detection of Shiga toxin producing Escherichia coli in food by polymerase chain reaction
In this study, three DNA extraction protocols using commercial kits (Instagene Matrix with Beckman Coulter Ampure XP Beads; Qiagen Gentra Puregene Yeast/Bact. Kit; Qiagen DNeasy Blood & Tissue) were assessed as alternative DNA extraction methods for the detection of the Shiga toxin gene by PCR in enrichments from sixteen different foods inoculated with STEC O157. The inoculated foods were bean sprouts, blackberries, blue cheese, cilantro, cocoa powder, coleslaw, cream of mushroom dried soup mix, cream of vegetable dried soup mix, flaxseed, guacamole, peanut butter, soft cheese, soy butter, spinach, walnut, and wheat fl...
Source: International Journal of Food Microbiology - July 20, 2023 Category: Food Science Authors: Tanis McMahon Mariam Abdelmesih Alexander Gill Source Type: research
A comprehensive review of the health benefits of flaxseed oil in relation to its chemical composition and comparison with other omega-3-rich oils
This study provides an overview of flaxseed oil effects with the reported action mechanisms related to its phytochemical composition and in comparison, to other PUFA-rich oils. This study presents the most updated and comprehensive review summarizing flaxseed oil's health benefits for the treatment of various diseases.PMID:37464425 | DOI:10.1186/s40001-023-01203-6 (Source: Atherosclerosis)
Source: Atherosclerosis - July 18, 2023 Category: Cardiology Authors: Somaia Al-Madhagy Naglaa S Ashmawy Ayat Mamdouh Omayma A Eldahshan Mohamed A Farag Source Type: research
Molecules, Vol. 28, Pages 5484: Health Benefits, Antioxidant Activity, and Sensory Attributes of Selected Cold-Pressed Oils
This study focused on the estimation and comparison of the physicochemical properties and sensory quality of six CPOs: linseed oil (CPLO), pumpkin oil (CPPO), milk thistle oil (CPMTO), rapeseed oil (CPRO), camelina oil (CPCO), and sunflower oil (CPSO), which are the most popular in the Polish market. These oils were analysed for their fatty acid composition (FAC), their tocopherol, sterol, polycyclic aromatic hydrocarbon (PAHs), water, and volatile matter (WVM) contents, as well as their antioxidant activity (AA) and oxidative stability parameters. Moreover, quantitative descriptive analysis (QDA) was performed to obtain d...
Source: Molecules - July 18, 2023 Category: Chemistry Authors: Dobrochna Rabiej-Kozio ł Monika Momot-Ruppert Barbara Stawicka Aleksandra Szyd łowska-Czerniak Tags: Article Source Type: research
A comprehensive review of the health benefits of flaxseed oil in relation to its chemical composition and comparison with other omega-3-rich oils
This study provides an overview of flaxseed oil effects with the reported a ction mechanisms related to its phytochemical composition and in comparison, to other PUFA-rich oils. This study presents the most updated and comprehensive review summarizing flaxseed oil’s health benefits for the treatment of various diseases.Graphical Abstract (Source: European Journal of Medical Research)
Source: European Journal of Medical Research - July 18, 2023 Category: Research Source Type: research
Genes, Vol. 14, Pages 1461: Molecular Advances to Combat Different Biotic and Abiotic Stresses in Linseed (Linum usitatissimum L.): A Comprehensive Review
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Shailja Chauhan
Flax, or linseed, is considered a “superfood”, which means that it is a food with diverse health benefits and potentially useful bioactive ingredients. It is a multi-purpose crop that is prized for its seed oil, fibre, nutraceutical, and probiotic qualities. It is suited to various habitats and agro-ecological conditions. Numerous abiotic and biotic stressors that can either have a direct or indirect impact on plant health are experienced by flax plants as a result of changing environmental circumstances. Research on the impact of various stresses and their possible ameli...
Source: Genes - July 17, 2023 Category: Genetics & Stem Cells Authors: Shruti Paliwal Manoj Kumar Tripathi Sushma Tiwari Niraj Tripathi Devendra K. Payasi Prakash N. Tiwari Kirti Singh Rakesh Kumar Yadav Ruchi Asati Shailja Chauhan Tags: Review Source Type: research
Cutinase production from Fusarium verticillioides using response surface methodology and its application as potential insecticide degrader
Environ Sci Pollut Res Int. 2023 Jul 14. doi: 10.1007/s11356-023-28635-1. Online ahead of print.ABSTRACTCutinase, a multifunctional enzyme, has shown great potential in environmental applications such as degradation of plastics and some commonly used insecticides. To overcome these environmental threatening problems, attempts should be made to enhance enzyme production. In the present study, a cutinolytic fungus was isolated from the soil. Based on 18 s rDNA sequencing, it was found that isolate AR08 belongs to the genus Fusarium and clades with Fusarium verticillioides. Optimization of medium composition for enhancement i...
Source: Environmental Science and Pollution Research International - July 14, 2023 Category: Environmental Health Authors: Aruna Singh Parmar Deepak K Rahi Source Type: research
Cutinase production from Fusarium verticillioides using response surface methodology and its application as potential insecticide degrader
Environ Sci Pollut Res Int. 2023 Jul 14. doi: 10.1007/s11356-023-28635-1. Online ahead of print.ABSTRACTCutinase, a multifunctional enzyme, has shown great potential in environmental applications such as degradation of plastics and some commonly used insecticides. To overcome these environmental threatening problems, attempts should be made to enhance enzyme production. In the present study, a cutinolytic fungus was isolated from the soil. Based on 18 s rDNA sequencing, it was found that isolate AR08 belongs to the genus Fusarium and clades with Fusarium verticillioides. Optimization of medium composition for enhancement i...
Source: Environmental Science and Pollution Research International - July 14, 2023 Category: Environmental Health Authors: Aruna Singh Parmar Deepak K Rahi Source Type: research
GSE213719 Alternative splicing and DNA methylation interact in linseed (Linum usitatissimum L.) response to different drought stresses
Contributors : Lei Wang ; Wei WangSeries Type : Methylation profiling by high throughput sequencingOrganism : Linum usitatissimumTo investigate changes in genome methylation in flax seedlings under drought stress, we selected a drought-tolerant flax variety (Z141) and a drought-sensitive flax variety (NY-17)We then performed genome methylation analysis using data obtained from Z141 and NY-17 leaf tissue BS-seq at four different treatments (DS, RW, RD and CK). (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - July 14, 2023 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Linum usitatissimum Source Type: research
The cardioprotective effects of secoisolariciresinol diglucoside (flaxseed lignan) against cafeteria diet-induced cardiac fibrosis and vascular injury in rats: an insight into apelin/AMPK/FOXO3a signaling pathways
Conclusion: FLE supplementation abrogated CAFD-induced cardiac injury and endothelial dysfunction in an apelin/APJ-dependent manner. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - July 11, 2023 Category: Drugs & Pharmacology Source Type: research
Sulphonated poly(ethersulfone)/carbon nano-onions-based nanocomposite membranes with high ion-conducting channels for salt removal via electrodialysis
Environ Sci Pollut Res Int. 2023 Jul 8. doi: 10.1007/s11356-023-28570-1. Online ahead of print.ABSTRACTHerein, we are reporting the carbon nano onions (CNO)-based sulphonated poly(ethersulfone) (SPES) composite membranes by varying CNO content in SPES matrix for water desalination applications. CNOs were cost-effectively synthesized using flaxseed oil as a carbon source in an energy efficient flame pyrolysis process. The physico- and electrochemical properties of nanocomposite membranes were evaluated and compared to pristine SPES. Moreover, the chemical characterisation of composite membranes and CNOs were illustrated usi...
Source: Environmental Science and Pollution Research International - July 8, 2023 Category: Environmental Health Authors: Poonam Kumari Prashant Upadhyay Kumud Malika Tripathi Ragini Gupta Vaibhav Kulshrestha Kamlendra Awasthi Source Type: research
Sulphonated poly(ethersulfone)/carbon nano-onions-based nanocomposite membranes with high ion-conducting channels for salt removal via electrodialysis
Environ Sci Pollut Res Int. 2023 Jul 8. doi: 10.1007/s11356-023-28570-1. Online ahead of print.ABSTRACTHerein, we are reporting the carbon nano onions (CNO)-based sulphonated poly(ethersulfone) (SPES) composite membranes by varying CNO content in SPES matrix for water desalination applications. CNOs were cost-effectively synthesized using flaxseed oil as a carbon source in an energy efficient flame pyrolysis process. The physico- and electrochemical properties of nanocomposite membranes were evaluated and compared to pristine SPES. Moreover, the chemical characterisation of composite membranes and CNOs were illustrated usi...
Source: Environmental Science and Pollution Research International - July 8, 2023 Category: Environmental Health Authors: Poonam Kumari Prashant Upadhyay Kumud Malika Tripathi Ragini Gupta Vaibhav Kulshrestha Kamlendra Awasthi Source Type: research
Erratum: Transcriptome profiling of flax plants exposed to a low-frequency alternating electromagnetic field
(Source: Frontiers in Genetics)
Source: Frontiers in Genetics - July 6, 2023 Category: Genetics & Stem Cells Source Type: research
Assessing the promoting effect of compound microbial agents on flax dew retting: Based on the relationship between metabolites and core genera
In this study, 16S rRNA sequencing and GC-MS (gas chromatography-mass spectrometry) techniques were employed to examine the relationship between bacterial succession and metabolite alterations during the dew retting process of flax. The results indicated that the addition of compound microbial agents may affect the production and transformation of metabolites by re-establishing bacterial communities and promoting the degradation of pectic substances and the release of metabolites, and the best retting effect was achieved under the combined addition (BA). In addition, Chryseobacterium, Bacillus, and Pseudoonas were closely ...
Source: Bioresource Technology - July 5, 2023 Category: Biotechnology Authors: Zhenchao Wu Jie Kang Chi Zhang Wen Zhang Jingping Ge Source Type: research
