GSE210680 Engineering Pluripotent DNA Methylation by CpG Island Methylation Response (CIMR) to Synthetic CpG-free ssDNA Insertion
Contributor : Joshua D TompkinsSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensIntegration of synthetic CpG Free DNA induces de novo DNAme in the flanking CpG island.Cellular differentiation requires global changes to DNA methylation (DNAme), where it functions to regulate transcription factor and chromatin remodeling activity, and genome interpretation. Here, we describe a simple DNAme engineering approach in pluripotent stem cells (PSCs), extending across large stretches of CpG dense “islands (CGIs).” Integration of synthetic CpG free single-stranded DNA (ssDNA) induces a target C...
Source: GEO: Gene Expression Omnibus - June 21, 2023 Category: Genetics & Stem Cells Tags: Methylation profiling by genome tiling array Homo sapiens Source Type: research
GSE210107 Differentiation of human cardiomyocyte and thymic epithelial cells from H1 control hESCs and H1 hESCs uniquely modified to be DNA methylated at the promoter CpG island of MLH1.
Contributor : Joshua TompkinsSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensEngineering Pluripotent DNA Methylation by CpG Island Methylation Response (CIMR) to Synthetic CpG-free ssDNA InsertionCellular differentiation requires global changes to DNA methylation (DNAme), where it functions to regulate transcription factor and chromatin remodeling activity, and genome interpretation. Here, we describe a simple DNAme engineering approach in pluripotent stem cells (PSCs), extending across large stretches of CpG dense “islands (CGIs).” Integration of synthetic CpG free single-st...
Source: GEO: Gene Expression Omnibus - June 21, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Intracranial Tumors in the First Year of Life
Adv Tech Stand Neurosurg. 2023;46:23-52. doi: 10.1007/978-3-031-28202-7_2.ABSTRACTIntracranial tumors in the first year of life are rare and, in this age group, are the second most common type of pediatric cancer after leukemias. As the more common solid tumor in neonates and infants, they present some peculiarities such as the high incidence of malignancies. Routine ultrasonography made easier to detect intrauterine tumors, but diagnosis can be delayed due to the lack or scarcity of recognizable symptoms. These neoplasms are often very large and highly vascular. Their removal is challenging, and there is a higher rate of ...
Source: Advances and Technical Standards in Neurosurgery - June 15, 2023 Category: Neurosurgery Authors: Jos é Francisco M Salomão Tatiana Protzenko Source Type: research
