Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis
Transgenic Res. 2023 Sep 13. doi: 10.1007/s11248-023-00367-5. Online ahead of print.ABSTRACTCitrus is one of the major horticultural crops with high economic and nutraceutical value. Despite the fact that conventional research has developed numerous improved varieties, citriculture is still susceptible to various stresses and requires innovative solutions such as genetic engineering. Among all the currently available modern approaches, Agrobacterium-mediated transformation is the most efficient method for introducing desired traits in citrus. However, being a non-host for Agrobacterium, various citrus species, including Ci...
Source: Transgenic Research - September 13, 2023 Category: Genetics & Stem Cells Authors: Sweta Singh Zeba Tarannum Sunil Kokane Dilip K Ghosh Ashwani K Sharma Harsh Chauhan Source Type: research
Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis
Transgenic Res. 2023 Sep 13. doi: 10.1007/s11248-023-00367-5. Online ahead of print.ABSTRACTCitrus is one of the major horticultural crops with high economic and nutraceutical value. Despite the fact that conventional research has developed numerous improved varieties, citriculture is still susceptible to various stresses and requires innovative solutions such as genetic engineering. Among all the currently available modern approaches, Agrobacterium-mediated transformation is the most efficient method for introducing desired traits in citrus. However, being a non-host for Agrobacterium, various citrus species, including Ci...
Source: Transgenic Research - September 13, 2023 Category: Genetics & Stem Cells Authors: Sweta Singh Zeba Tarannum Sunil Kokane Dilip K Ghosh Ashwani K Sharma Harsh Chauhan Source Type: research
Comparative analysis of bile metabolic profile in patients with biliary obstruction complicated by Clonorchis sinensis infection
ConclusionThese results show that there were significant differences between OB+C.s and OB patients, especially in amino acids. The metabolic signature and perturbations in metabolic pathways may help to better distinguish OB+C.s and OB patients. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - September 12, 2023 Category: Microbiology Source Type: research
Insights into the physiological, molecular, and genetic regulators of albinism in Camellia sinensis leaves
Introduction: Yanling Yinbiancha, a cultivar of Camellia sinensis (L.) O. Kuntze, is an evergreen woody perennial with characteristic albino leaves. A mutant variant with green leaves on branches has been recently identified. The molecular mechanisms underlying this color variation remain unknown.Methods: We aimed to utilize omics tools to decipher the molecular basis for this color variation, with the ultimate goal of enhancing existing germplasm and utilizing it in future breeding programs.Results and discussion: Albinotic leaves exhibited significant chloroplast degeneration and reduced carotenoid accumulation. Transcri...
Source: Frontiers in Genetics - September 6, 2023 Category: Genetics & Stem Cells Source Type: research
Production of chitosan-PVA packaging films using green synthesized ZnO NPs enriched with dragon fruit waste extract envisaging food packaging applications
Int J Biol Macromol. 2023 Aug 21:126457. doi: 10.1016/j.ijbiomac.2023.126457. Online ahead of print.ABSTRACTIn this work, the PVA-CS composite packaging films doped with biomass fabricated zinc oxide nanoparticles (ZnO NPs) and dragon fruit waste extract (DFE) were produced for potential use in food packaging applications. ZnO NPs were synthesized using a sustainable method employing C. sinensis waste extract as a reducing agent. Chitosan and PVA were blended in a specific ratio (1: 1 w/w) to obtain a film-forming solution, into which the ZnO NPs and dragon fruit waste extract were incorporated. The resulting solution was ...
Source: International Journal of Biological Macromolecules - August 23, 2023 Category: Biochemistry Authors: None Shahid-Ul-Islam Vivek Jaiswal B S Butola Abhijeet Majumdar Source Type: research
Production of chitosan-PVA packaging films using green synthesized ZnO NPs enriched with dragon fruit waste extract envisaging food packaging applications
Int J Biol Macromol. 2023 Aug 21:126457. doi: 10.1016/j.ijbiomac.2023.126457. Online ahead of print.ABSTRACTIn this work, the PVA-CS composite packaging films doped with biomass fabricated zinc oxide nanoparticles (ZnO NPs) and dragon fruit waste extract (DFE) were produced for potential use in food packaging applications. ZnO NPs were synthesized using a sustainable method employing C. sinensis waste extract as a reducing agent. Chitosan and PVA were blended in a specific ratio (1: 1 w/w) to obtain a film-forming solution, into which the ZnO NPs and dragon fruit waste extract were incorporated. The resulting solution was ...
Source: International Journal of Biological Macromolecules - August 23, 2023 Category: Biochemistry Authors: None Shahid-Ul-Islam Vivek Jaiswal B S Butola Abhijeet Majumdar Source Type: research
Morphological and phylogenetic analyzes reveal two new species of Melanconiella from Fujian Province, China
DiscussionThese data identify new species of Melanconiella, expanding the potential range and distribution of these dark septate fungi. The developed keys provide a reference source for further characterization of these fungi. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - August 16, 2023 Category: Microbiology Source Type: research
Multilayer omics reveals the molecular mechanism of early infection of Clonorchis sinensis juvenile
Clonorchiasis remains a non-negligible global zoonosis, causing serious socioeconomic burdens in endemic areas. Clonorchis sinensis infection typically elicits Th1/Th2 mixed immune responses during the course of ... (Source: Parasites and Vectors)
Source: Parasites and Vectors - August 16, 2023 Category: Microbiology Authors: Yuhong Wu, Xueling Deng, Zhanshuai Wu, Dengyu Liu, Xiaoyin Fu, Lili Tang, Shanshan He, Jiahui Lv, Jilong Wang, Qing Li, Tingzheng Zhan and Zeli Tang Tags: Research Source Type: research
Rapid, sensitive and visual detection of Clonorchis sinensis with an RPA-CRISPR/Cas12a-based dual readout portable platform
In this study, an RPA-CRISPR/Cas12a-based dual readout portable detection platform was established, which shows high sensitivity (one copy/μl) and specificity (no cross-reactivity with common pathogens) by rapid preamplification and combines lateral flow strips and visual fluorescence for visualization of results by the naked eye within 1 h. Moreover, 50 human fecal swabs and 50 fish flesh samples were detected by this platform and nested PCR. The CRISPR/Cas12a-based dual readout portable platform showed 10.0 % (5/50) C. sinensis-positive samples in human fecal swabs and 28.0 % (14/50) in fish flesh, which was consistent ...
Source: International Journal of Biological Macromolecules - July 26, 2023 Category: Biochemistry Authors: Taojun Huang Lu Li Jianhua Li Xin Li Shan Li Xiaocen Wang Nan Zhang Yanhui Yu Xichen Zhang Zhiteng Zhao Yanbing Guo Lili Cao Pengtao Gong Source Type: research
Rapid, sensitive and visual detection of Clonorchis sinensis with an RPA-CRISPR/Cas12a-based dual readout portable platform
In this study, an RPA-CRISPR/Cas12a-based dual readout portable detection platform was established, which shows high sensitivity (one copy/μl) and specificity (no cross-reactivity with common pathogens) by rapid preamplification and combines lateral flow strips and visual fluorescence for visualization of results by the naked eye within 1 h. Moreover, 50 human fecal swabs and 50 fish flesh samples were detected by this platform and nested PCR. The CRISPR/Cas12a-based dual readout portable platform showed 10.0 % (5/50) C. sinensis-positive samples in human fecal swabs and 28.0 % (14/50) in fish flesh, which was consistent ...
Source: International Journal of Biological Macromolecules - July 26, 2023 Category: Biochemistry Authors: Taojun Huang Lu Li Jianhua Li Xin Li Shan Li Xiaocen Wang Nan Zhang Yanhui Yu Xichen Zhang Zhiteng Zhao Yanbing Guo Lili Cao Pengtao Gong Source Type: research
Recombinant protein EBI3 attenuates Clonorchis sinensis-induced liver fibrosis by inhibiting hepatic stellate cell activation in mice
Chronic infection with Clonorchis sinensis can cause hepatobiliary fibrosis and even lead to hepatobiliary carcinoma. Epstein-Barr virus-induced gene 3 protein (EBI3) is a subunit of interleukin 35, which can reg... (Source: Parasites and Vectors)
Source: Parasites and Vectors - July 21, 2023 Category: Microbiology Authors: Lei Zhao, Jia Li, Gang Mo, Deping Cao, Chun Li, Guoyang Huang, Liping Jiang, Gen Chen, Hongbing Yao and Xiaohong Peng Tags: Research Source Type: research
Genes, Vol. 14, Pages 1417: Haplotype-Resolution Transcriptome Analysis Reveals Important Responsive Gene Modules and Allele-Specific Expression Contributions under Continuous Salt and Drought in Camellia sinensis
This study presents a high-quality genome-based transcriptome dynamic analysis of C. sinensis’ response to salt and drought stress. A total of 2244 upregulated and 2164 downregulated genes were identified under salt and drought stress compared to the control sample. Most of the differentially expression genes (DEGs) were found to involve divergent regulation processes at different time points under stress. Some shared up- and downregulated DEGs related to secondary metabolic and photosynthetic processes, respectively. Weighted gene co-expression network analysis (WGCNA) revealed six co-expression modules sign...
Source: Genes - July 8, 2023 Category: Genetics & Stem Cells Authors: Qing Zhang Ziqi Ye Yinghao Wang Xingtan Zhang Weilong Kong Tags: Article Source Type: research
Revealing ecotype influences on Cistanche sinensis: from the perspective of endophytes to metabolites characteristics
DiscussionThe aim of this research is: (1) to explore firstly the influence of ecotype on C. sinensis from the perspective of endophytes and metabolites; (2) to investigate the relationship between endophytes and metabolites. This discovery advances our understanding of the interaction between endophytes and plants and provides a theoretical basis for cultivation of C. sinensis in future. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - June 27, 2023 Category: Microbiology Source Type: research
Establishment of an efficient transformation system and its application in regulatory mechanism analysis of biological macromolecules in tea plants
In this study, we established a highly efficient, labor-saving, and cost-effective Agrobacterium rhizogenes-mediated hairy roots genetic transformation system for C. sinensis, which can be used for gene overexpression and genome editing. The established transformation system was simple to operate, bypassing tissue culture and antibiotic screening, and only took two months to complete. We used this system to conduct function analysis of transcription factor CsMYB73 and found that CsMYB73 negatively regulates L-theanine synthesis in tea plant. Additionally, callus formation was successfully induced using transgenic roots, an...
Source: International Journal of Biological Macromolecules - June 15, 2023 Category: Biochemistry Authors: Haijie Ma Ningge Liu Xuepeng Sun Mengling Zhu Tingfeng Mao Suya Huang Xinyue Meng Hangfei Li Min Wang Huiling Liang Source Type: research
Coraliomargarita parva sp. nov., isolated from mangrove sediment and genome-based analysis of the class Opitutae revealed five novel families: Coraliomargaritaceae fam. nov., Pelagicoccaceae fam. nov., Cerasicoccaeae fam. nov., Oceanipulchritudinaceae fam. nov., and Alterococcaeae fam. nov.
This study provided a new taxonomic framework of the class Opitutae based on the genomic data. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - June 9, 2023 Category: Microbiology Source Type: research
