Anticoagulant and antiplatelet activity of aqueous extracts of Citrus sinensis and Lippia alba: interactions and potential for the development of antithrombotics
Citrus sinensis and Lippia alba are herbal medicines widely used in the form of tea (infusion, decoction), which ethanolic extracts have already shown great anticoagulant activity in vitro. For this reason, they seem to be excellent candidates for the development of new antithrombotics and also have the potential to interact with them. The aim of this study was to evaluate the activity of aqueous extracts in blood coagulation and platelet aggregation, in addition to analysing the micromolecular composition of these species. Thrombin generation test (TGT) by the Calibrated Automated Thrombogram method and Platelet Agg...
Source: Blood Coagulation and Fibrinolysis - November 26, 2022 Category: Hematology Tags: ORIGINAL ARTICLES Source Type: research

Comprehensive genome-wide identification and expression profiling of ADF gene family in Citrus sinensis, induced by endophytic colonization of Beauveria bassiana
Int J Biol Macromol. 2022 Nov 17:S0141-8130(22)02712-X. doi: 10.1016/j.ijbiomac.2022.11.153. Online ahead of print.ABSTRACTEndophytic entomopathogenic species are known to systematically colonize host plants and form symbiotic associations that benefit the plants they live with. The actin-depolymerizing factors (ADFs) are a group of gene family that regulate growth, development, and defense-related functions in plants. Systematic studies of ADF family at the genome-wide level and their expression in response to endophytic colonization are essential to understand its functions but are currently lacking in this field. 14ADF ...
Source: International Journal of Biological Macromolecules - November 20, 2022 Category: Biochemistry Authors: Luis Carlos Ramos Aguila Jessica Paola S ánchez Moreano Komivi Senyo Akutse Bamisope Steve Bamisile Juxiu Liu Fasih Ullah Haider Hafiza Javaira Ashraf Liande Wang Source Type: research

Comprehensive genome-wide identification and expression profiling of ADF gene family in Citrus sinensis, induced by endophytic colonization of Beauveria bassiana
Int J Biol Macromol. 2022 Nov 17:S0141-8130(22)02712-X. doi: 10.1016/j.ijbiomac.2022.11.153. Online ahead of print.ABSTRACTEndophytic entomopathogenic species are known to systematically colonize host plants and form symbiotic associations that benefit the plants they live with. The actin-depolymerizing factors (ADFs) are a group of gene family that regulate growth, development, and defense-related functions in plants. Systematic studies of ADF family at the genome-wide level and their expression in response to endophytic colonization are essential to understand its functions but are currently lacking in this field. 14ADF ...
Source: International Journal of Biological Macromolecules - November 20, 2022 Category: Biochemistry Authors: Luis Carlos Ramos Aguila Jessica Paola S ánchez Moreano Komivi Senyo Akutse Bamisope Steve Bamisile Juxiu Liu Fasih Ullah Haider Hafiza Javaira Ashraf Liande Wang Source Type: research

Structure-based virtual screening and molecular dynamics of potential inhibitors targeting sodium-bile acid co-transporter of carcinogenic liver fluke < i > Clonorchis sinensis < /i >
ConclusionsIt is proposed that compound 9806452 act as a potential inhibitor toward CsSBAT and further studies are warranted for drug development process against clonorchiasis. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - November 9, 2022 Category: Tropical Medicine Authors: Won Gi Yoo Source Type: research

Genes, Vol. 13, Pages 1994: Metabolomic and Transcriptomic Analyses Reveal the Characteristics of Tea Flavonoids and Caffeine Accumulation and Regulation between Chinese Varieties (Camellia sinensis var. sinensis) and Assam Varieties (C. sinensis var. assamica)
In this study, metabolomic and transcriptomic analyses were performed to investigate the differences of flavonoids and caffeine accumulation and regulation between Chinese varieties, including the &amp;lsquo;BTSC&amp;rsquo; group with green leaf, the &amp;lsquo;BTZY&amp;rsquo; group with purple foliage, and the &amp;lsquo;MYC&amp;rsquo; group comprising Assam varieties with green leaf. The results showed that most of the flavonoids were down-regulated in the &amp;lsquo;MYC&amp;rsquo; group; however, the total anthocyanin contents were higher than that of the &amp;lsquo;BTSC&amp;rsquo...
Source: Genes - October 31, 2022 Category: Genetics & Stem Cells Authors: Hao Tang Man Zhang Jiayu Liu Jiao Cai Tags: Article Source Type: research

Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii
This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.PMID:36280206 | DOI:10.1016/j.actatropica.2022.106729 (Source: Acta Tropica)
Source: Acta Tropica - October 24, 2022 Category: Infectious Diseases Authors: Lenu B Fakae Mohammad S R Harun Darren Shu Jeng Ting Harminder S Dua Gareth W V Cave Xing-Quan Zhu Carl W Stevenson Hany M Elsheikha Source Type: research

Camellia sinensis solvent extract, epigallocatechin gallate and caffeine confer trophocidal and cysticidal effects against Acanthamoeba castellanii
This study shows the anti-acanthamoebic potential of C. sinensis SE against A. castellanii trophozoites and cysts. Pre-clinical studies are required to elucidate the in vivo efficacy and safety of C. sinensis SE.PMID:36280206 | DOI:10.1016/j.actatropica.2022.106729 (Source: Acta Tropica)
Source: Acta Tropica - October 24, 2022 Category: Infectious Diseases Authors: Lenu B Fakae Mohammad S R Harun Darren Shu Jeng Ting Harminder S Dua Gareth W V Cave Xing-Quan Zhu Carl W Stevenson Hany M Elsheikha Source Type: research

Interleukin-33 deficiency prevents biliary injuries and repairments caused by Clonorchis sinensis via restraining type 2 cytokines
Clonorchiasis caused by Clonorchis sinensis is a zoonotic parasitic disease characterized by cholangitis, biliary proliferation, biliary fibrosis, and even cholangiocarcinoma. Our previous study showed that the e... (Source: Parasites and Vectors)
Source: Parasites and Vectors - October 22, 2022 Category: Microbiology Authors: Chao Yan, Na Xu, Man Liu, Zhihua Jiang, Jing Wu, Stephane Koda, Yu Chen, Beibei Zhang, Qian Yu, Yin-Hai Xu, Jian-Lin Wu and Kui-Yang Zheng Tags: Research Source Type: research

Clonorchis sinensis infection contributes to hepatocellular carcinoma progression in rat
This study explored the potential relationship between C. sinensis infection and HCC. A total of 110 Sprague-Dawley rats were divided into four treatment groups, the negative control group (NC) received intragastric (i.g.) administration of saline, while the clonorchiasis group (CS) received i.g. administration of 150 C. sinensis metacercariae. The diethylnitrosamine-induced group (DEN) received intraperitoneal (i.p.) administration of DEN. The clonorchiasis DEN-induced group (CSDEN) received i.g. administration of 150 C. sinensis metacercariae followed by i.p. administration of DEN. Hematoxylin and eosin staining, immunoh...
Source: Cell Research - October 20, 2022 Category: Cytology Authors: Yapeng Qi Junwen Hu Jiahao Liang Xiaoyin Hu Ning Ma Bangde Xiang Source Type: research

Clonorchis sinensis infection contributes to hepatocellular carcinoma progression in rat
This study explored the potential relationship between C. sinensis infection and HCC. A total of 110 Sprague-Dawley rats were divided into four treatment groups, the negative control group (NC) received intragastric (i.g.) administration of saline, while the clonorchiasis group (CS) received i.g. administration of 150 C. sinensis metacercariae. The diethylnitrosamine-induced group (DEN) received intraperitoneal (i.p.) administration of DEN. The clonorchiasis DEN-induced group (CSDEN) received i.g. administration of 150 C. sinensis metacercariae followed by i.p. administration of DEN. Hematoxylin and eosin staining, immunoh...
Source: Cancer Control - October 20, 2022 Category: Cancer & Oncology Authors: Yapeng Qi Junwen Hu Jiahao Liang Xiaoyin Hu Ning Ma Bangde Xiang Source Type: research

Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2
Clonorchis sinensis (C. sinensis) infection induces severe hepatobiliary injuries, which can cause inflammation, periductal fibrosis, and even cholangiocarcinoma. Sphingolipid metabolic pathways responsible for the generation of sphingosine-1-phosphate (S1P) and its receptor S1P receptors (S1PRs) have been implicated in many liver-related diseases. However, the role of S1PRs in C. sinensis-mediated biliary epithelial cells (BECs) proliferation and hepatobiliary injury has not been elucidated. In the present study, we found that C. sinensis infection resulted in alteration of bioactive lipids and sphingolipid metabolic path...
Source: Frontiers in cellular and infection microbiology - October 19, 2022 Category: Microbiology Source Type: research

Can avian flyways reflect dispersal barriers of clinostomid parasites? First evidence from the mitogenome of Clinostomum complanatum
Gene. 2022 Oct 12:146952. doi: 10.1016/j.gene.2022.146952. Online ahead of print.ABSTRACTClinostomum complanatum (Rudolphi, 1814) is an economically important parasitic flatworm (Trematoda, Digenea), yet little is known on the population structure of these animals. We characterise a new mitochondrial genome for C. complanatum, derived from an Iranian specimen. The newly obtained sequence is used to position the species in the digenean tree of life. The first-ever intraspecific comparison at mitogenome scale within C. complanatum revealed a high degree of similarity to the previously sequenced mitogenome of a distant (Itali...
Source: Gene - October 15, 2022 Category: Genetics & Stem Cells Authors: Marlies Monnens Ali Halajian D Tim J Littlewood Andrew G Briscoe Tom Artois Maarten P M Vanhove Source Type: research

Can avian flyways reflect dispersal barriers of clinostomid parasites? First evidence from the mitogenome of Clinostomum complanatum
Gene. 2022 Oct 12:146952. doi: 10.1016/j.gene.2022.146952. Online ahead of print.ABSTRACTClinostomum complanatum (Rudolphi, 1814) is an economically important parasitic flatworm (Trematoda, Digenea), yet little is known on the population structure of these animals. We characterise a new mitochondrial genome for C. complanatum, derived from an Iranian specimen. The newly obtained sequence is used to position the species in the digenean tree of life. The first-ever intraspecific comparison at mitogenome scale within C. complanatum revealed a high degree of similarity to the previously sequenced mitogenome of a distant (Itali...
Source: Gene - October 15, 2022 Category: Genetics & Stem Cells Authors: Marlies Monnens Ali Halajian D Tim J Littlewood Andrew G Briscoe Tom Artois Maarten P M Vanhove Source Type: research

IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
Clonorchiasis caused by Clonorchis sinensis is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive role during infection. Our previous study found that IL-10 was increased in mice with C. sinensis infection. However, the role and mechanism of IL-10 playing in hepatobiliary injury induced by C. sinensis infection remain unknown. Herein, Il10+/+ mice and Il10+/- C57BL/6J mice were infected with C. sinensis. It was found that IL-10...
Source: Frontiers in cellular and infection microbiology - October 13, 2022 Category: Microbiology Source Type: research

Pangenome-wide analysis of cyclic nucleotide-gated channel (CNGC) gene family in citrus Spp. Revealed their intraspecies diversity and potential roles in abiotic stress tolerance
Cyclic nucleotide-gated channels (CNGC) gene family has been found to be involved in physiological processes including signaling pathways, environmental stresses, plant growth, and development. This gene family of non-selective cation channels is known to regulate the uptake of calcium and is reported in several plant species. The pangenome-wide studies enable researchers to understand the genetic diversity comprehensively; as a comparative analysis of multiple plant species or member of a species at once helps to better understand the evolutionary relationships and diversity present among them. In the current study, pange...
Source: Frontiers in Genetics - October 11, 2022 Category: Genetics & Stem Cells Source Type: research