Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region
Transgenic Res. 2024 Apr 10. doi: 10.1007/s11248-024-00379-9. Online ahead of print.ABSTRACTBanana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national income to primary banana producers in the SEA region such as the Philippines, Indonesia, Thailand, Vietnam, and Malaysia. However, the widely traded banana industry is plagued by numerous threats including pests and diseases, post-harvest issues and extreme climate vulnerability. To address these challenges, new breeding techniques such as gene editing have been explored for breeding prog...
Source: Transgenic Research - April 10, 2024 Category: Genetics & Stem Cells Authors: Nurzatil Sharleeza Mat Jalaluddin Abdulah Al-Hadi Ahmad Fuaad Rofina Yasmin Othman Source Type: research
Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region
Transgenic Res. 2024 Apr 10. doi: 10.1007/s11248-024-00379-9. Online ahead of print.ABSTRACTBanana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national income to primary banana producers in the SEA region such as the Philippines, Indonesia, Thailand, Vietnam, and Malaysia. However, the widely traded banana industry is plagued by numerous threats including pests and diseases, post-harvest issues and extreme climate vulnerability. To address these challenges, new breeding techniques such as gene editing have been explored for breeding prog...
Source: Transgenic Research - April 10, 2024 Category: Genetics & Stem Cells Authors: Nurzatil Sharleeza Mat Jalaluddin Abdulah Al-Hadi Ahmad Fuaad Rofina Yasmin Othman Source Type: research
Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region
Transgenic Res. 2024 Apr 10. doi: 10.1007/s11248-024-00379-9. Online ahead of print.ABSTRACTBanana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national income to primary banana producers in the SEA region such as the Philippines, Indonesia, Thailand, Vietnam, and Malaysia. However, the widely traded banana industry is plagued by numerous threats including pests and diseases, post-harvest issues and extreme climate vulnerability. To address these challenges, new breeding techniques such as gene editing have been explored for breeding prog...
Source: Transgenic Research - April 10, 2024 Category: Genetics & Stem Cells Authors: Nurzatil Sharleeza Mat Jalaluddin Abdulah Al-Hadi Ahmad Fuaad Rofina Yasmin Othman Source Type: research
Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region
Transgenic Res. 2024 Apr 10. doi: 10.1007/s11248-024-00379-9. Online ahead of print.ABSTRACTBanana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national income to primary banana producers in the SEA region such as the Philippines, Indonesia, Thailand, Vietnam, and Malaysia. However, the widely traded banana industry is plagued by numerous threats including pests and diseases, post-harvest issues and extreme climate vulnerability. To address these challenges, new breeding techniques such as gene editing have been explored for breeding prog...
Source: Transgenic Research - April 10, 2024 Category: Genetics & Stem Cells Authors: Nurzatil Sharleeza Mat Jalaluddin Abdulah Al-Hadi Ahmad Fuaad Rofina Yasmin Othman Source Type: research
Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Transgenic Res. 2024 Apr 5. doi: 10.1007/s11248-024-00378-w. Online ahead of print.ABSTRACTGenetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to furthe...
Source: Transgenic Research - April 5, 2024 Category: Genetics & Stem Cells Authors: Michael Meissle Source Type: research
Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Transgenic Res. 2024 Apr 5. doi: 10.1007/s11248-024-00378-w. Online ahead of print.ABSTRACTGenetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to furthe...
Source: Transgenic Research - April 5, 2024 Category: Genetics & Stem Cells Authors: Michael Meissle Source Type: research
Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Transgenic Res. 2024 Apr 5. doi: 10.1007/s11248-024-00378-w. Online ahead of print.ABSTRACTGenetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to furthe...
Source: Transgenic Research - April 5, 2024 Category: Genetics & Stem Cells Authors: Michael Meissle Source Type: research
Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Transgenic Res. 2024 Apr 5. doi: 10.1007/s11248-024-00378-w. Online ahead of print.ABSTRACTGenetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to furthe...
Source: Transgenic Research - April 5, 2024 Category: Genetics & Stem Cells Authors: Michael Meissle Source Type: research
Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Transgenic Res. 2024 Apr 5. doi: 10.1007/s11248-024-00378-w. Online ahead of print.ABSTRACTGenetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking pests, such as Lygus spp. (Hemiptera: Miridae) and thrips (Thysanoptera). Ingesting high doses of the insecticidal protein resulted in adverse effects on life table parameters of beneficial, predatory Orius spp. (Hemiptera: Anthocoridae). This triggered laboratory studies with more realistic food treatments, including different combinations of prey types with and without Bt protein to furthe...
Source: Transgenic Research - April 5, 2024 Category: Genetics & Stem Cells Authors: Michael Meissle Source Type: research
The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens
Transgenic Res. 2024 Apr 4. doi: 10.1007/s11248-024-00381-1. Online ahead of print.ABSTRACTGenome editing via CRISPR/Cas has enabled targeted genetic modifications in various species, including plants. The requirement for specific protospacer-adjacent motifs (PAMs) near the target gene, as seen with Cas nucleases like SpCas9, limits its application. PAMless SpCas9 variants, designed with a relaxed PAM requirement, have widened targeting options. However, these so-call PAMless SpCas9 still show variation of editing efficiency depending on the PAM and their efficiency lags behind the native SpCas9. Here we assess the potenti...
Source: Transgenic Research - April 4, 2024 Category: Genetics & Stem Cells Authors: Julie Calbry Guillaume Goudounet Florence Charlot Anouchka Guyon-Debast Pierre-Fran çois Perroud Fabien Nogu é Source Type: research
Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco
Transgenic Res. 2024 Apr 4. doi: 10.1007/s11248-024-00375-z. Online ahead of print.ABSTRACTPlants can produce complex pharmaceutical and technical proteins. Spider silk proteins are one example of the latter and can be used, for example, as compounds for high-performance textiles or wound dressings. If genetically fused to elastin-like polypeptides (ELPs), the silk proteins can be reversibly precipitated from clarified plant extracts at moderate temperatures of ~ 30 °C together with salt concentrations > 1.5 M, which simplifies purification and thus reduces costs. However, the technologies developed around this mechani...
Source: Transgenic Research - April 4, 2024 Category: Genetics & Stem Cells Authors: H M Gruchow P Opdensteinen J F Buyel Source Type: research
The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens
Transgenic Res. 2024 Apr 4. doi: 10.1007/s11248-024-00381-1. Online ahead of print.ABSTRACTGenome editing via CRISPR/Cas has enabled targeted genetic modifications in various species, including plants. The requirement for specific protospacer-adjacent motifs (PAMs) near the target gene, as seen with Cas nucleases like SpCas9, limits its application. PAMless SpCas9 variants, designed with a relaxed PAM requirement, have widened targeting options. However, these so-call PAMless SpCas9 still show variation of editing efficiency depending on the PAM and their efficiency lags behind the native SpCas9. Here we assess the potenti...
Source: Transgenic Research - April 4, 2024 Category: Genetics & Stem Cells Authors: Julie Calbry Guillaume Goudounet Florence Charlot Anouchka Guyon-Debast Pierre-Fran çois Perroud Fabien Nogu é Source Type: research
Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco
Transgenic Res. 2024 Apr 4. doi: 10.1007/s11248-024-00375-z. Online ahead of print.ABSTRACTPlants can produce complex pharmaceutical and technical proteins. Spider silk proteins are one example of the latter and can be used, for example, as compounds for high-performance textiles or wound dressings. If genetically fused to elastin-like polypeptides (ELPs), the silk proteins can be reversibly precipitated from clarified plant extracts at moderate temperatures of ~ 30 °C together with salt concentrations > 1.5 M, which simplifies purification and thus reduces costs. However, the technologies developed around this mechani...
Source: Transgenic Research - April 4, 2024 Category: Genetics & Stem Cells Authors: H M Gruchow P Opdensteinen J F Buyel Source Type: research
Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene
In this study, we present a new approach employing a multi-intron containing Cas9 gene plus multiple gRNAs separated with tRNA sequences to target the phytoene desaturase gene in both 'Carrizo' citrange and 'Duncan' grapefruit. Notably, using this unified vector significantly boosted editing efficiency in both citrus varieties, showcasing mutations in all three designated targets. The implementation of this multiplex gene editing system with a multi-intron-containing Cas9 plus a gRNA-tRNA array demonstrates a promising avenue for efficient citrus genome editing, equipping us with potent tools in the ongoing battle against ...
Source: Transgenic Research - April 2, 2024 Category: Genetics & Stem Cells Authors: Poulami Sarkar Jorge Santiago Vazquez Mingxi Zhou Amit Levy Zhonglin Mou Vladimir Orbovi ć Source Type: research
Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene
In this study, we present a new approach employing a multi-intron containing Cas9 gene plus multiple gRNAs separated with tRNA sequences to target the phytoene desaturase gene in both 'Carrizo' citrange and 'Duncan' grapefruit. Notably, using this unified vector significantly boosted editing efficiency in both citrus varieties, showcasing mutations in all three designated targets. The implementation of this multiplex gene editing system with a multi-intron-containing Cas9 plus a gRNA-tRNA array demonstrates a promising avenue for efficient citrus genome editing, equipping us with potent tools in the ongoing battle against ...
Source: Transgenic Research - April 2, 2024 Category: Genetics & Stem Cells Authors: Poulami Sarkar Jorge Santiago Vazquez Mingxi Zhou Amit Levy Zhonglin Mou Vladimir Orbovi ć Source Type: research
