Burkholderia pseudomallei in Soil, US Virgin Islands, 2019.
Abstract The distribution of Burkholderia pseudomallei in the Caribbean is poorly understood. We isolated B. pseudomallei from US Virgin Islands soil. The soil isolate was genetically similar to other isolates from the Caribbean, suggesting that B. pseudomallei might have been introduced to the islands multiple times through severe weather events. PMID: 33079041 [PubMed - as supplied by publisher] (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - October 21, 2020 Category: Infectious Diseases Authors: Stone NE, Hall CM, Browne AS, Sahl JW, Hutton SM, Santana-Propper E, Celona KR, Guendel I, Harrison CJ, Gee JE, Elrod MG, Busch JD, Hoffmaster AR, Ellis EM, Wagner DM Tags: Emerg Infect Dis Source Type: research

Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories.
Abstract The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories. PM...
Source: Emerging Infectious Diseases - October 21, 2020 Category: Infectious Diseases Authors: Kesterson AE, Craig JE, Chuvala LJ, Heine HS Tags: Emerg Infect Dis Source Type: research

Secondary metabolites from the Burkholderia pseudomallei complex: structure, ecology, and evolution.
er JR Abstract Bacterial secondary metabolites play important roles in promoting survival, though few have been carefully studied in their natural context. Numerous gene clusters code for secondary metabolites in the genomes of members of the Bptm group, made up of three closely related species with distinctly different lifestyles: the opportunistic pathogen Burkholderia pseudomallei, the non-pathogenic saprophyte Burkholderia thailandensis, and the host-adapted pathogen Burkholderia mallei. Several biosynthetic gene clusters are conserved across two or all three species, and this provides an opportunity to unders...
Source: Journal of Industrial Microbiology and Biotechnology - October 14, 2020 Category: Microbiology Authors: Klaus JR, Coulon PML, Koirala P, Seyedsayamdost MR, Déziel E, Chandler JR Tags: J Ind Microbiol Biotechnol Source Type: research

Burkholderia pseudomallei in Soil, US Virgin Islands, 2019
N. E. Stone et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - October 8, 2020 Category: Infectious Diseases Source Type: research

Chronic melioidotic osteomyelitis presenting as suspected sarcoma
Melioidosis is an infection caused by Gram-negative bacillus Burkholderia pseudomallei commonly present in the water and soil (Limmathurotsakul et al., 2013; Benoit et al., 2015). Clinically melioidosis can mimic other diseases such as cancer and tuberculosis, which in our case was suspected sarcoma. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - October 8, 2020 Category: Infectious Diseases Authors: Samuel S.Y. Wang Tags: Medical Imagery Source Type: research

Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers.
This article is protected by copyright. All rights reserved. PMID: 33006278 [PubMed - as supplied by publisher] (Source: Biotechnology Journal)
Source: Biotechnology Journal - September 30, 2020 Category: Biotechnology Authors: Datta P, Yan L, Awofiranye A, Dordick JS, Linhardt RJ Tags: Biotechnol J Source Type: research

In vitro  production of ergothioneine isotopologues.
We describe a general protocol for the production of ergothioneine isotopologues with programmable 2H, 15N 13C, 34S and 33S isotope labelling patterns. This enzyme-based approach makes efficient use of commercial isotope reagents and is also directly applicable for the synthesis of radio-isotopologues. PMID: 32996678 [PubMed - as supplied by publisher] (Source: Angewandte Chemie)
Source: Angewandte Chemie - September 29, 2020 Category: Chemistry Authors: Beliaeva MA, Burn R, Lim D, Seebeck FP Tags: Angew Chem Int Ed Engl Source Type: research

Genomic loss in environmental and isogenic morphotype isolates of < i > Burkholderia pseudomallei < /i > is associated with intracellular survival and plaque-forming efficiency
Conclusions/SignificanceOur plaque screening and genomic studies revealed evidence of impairment in plaque formation in environmental isolates ofB.pseudomallei that is associated with large genomic loss of genes important for intracellular multiplication and MNGC formation. These findings suggest that the genomic and phenotypic differences of environmental isolates may be associated with clinical infection. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - September 28, 2020 Category: Tropical Medicine Authors: Natnaree Saiprom Source Type: research

Investigation of Melioidosis Using Blood Culture and Indirect Hemagglutination Assay Serology among Patients with Fever, Northern Tanzania.
Abstract Prediction models indicate that melioidosis may be common in parts of East Africa, but there are few empiric data. We evaluated the prevalence of melioidosis among patients presenting with fever to hospitals in Tanzania. Patients with fever were enrolled at two referral hospitals in Moshi, Tanzania, during 2007-2008, 2012-2014, and 2016-2019. Blood was collected from participants for aerobic culture. Bloodstream isolates were identified by conventional biochemical methods. Non-glucose-fermenting Gram-negative bacilli were further tested using a Burkholderia pseudomallei latex agglutination assay. Also, we...
Source: The American Journal of Tropical Medicine and Hygiene - September 27, 2020 Category: Tropical Medicine Authors: Maze MJ, Elrod MG, Biggs HM, Bonnewell J, Carugati M, Hoffmaster AR, Lwezaula BF, Madut DB, Maro VP, Mmbaga BT, Morrissey AB, Saganda W, Sakasaka P, Rubach MP, Crump JA Tags: Am J Trop Med Hyg Source Type: research

Benzylaminoethylureido-tailed benzenesulfonamides show potent inhibitory action against bacterial carbonic anhydrases.
Abstract A series of benzylaminoethylureido-tailed benzenesulfonamides was analyzed for their inhibition potential against bacterial carbonic anhydrases (CAs) such as VhCA α, β, and γ from Vibrio cholerae , and BpsCA β and γ-CAs from Burkholderia pseudomallei . Growing drug-resistance against antibiotics demands alternative targets and mechanisms of action. As CA is essential for the survival of bacteria, such enzymes have the potential for developing new antibiotics. Most of the compounds presented excellent inhibition potential against VhCA γ as compared to α and β, w...
Source: ChemMedChem - September 22, 2020 Category: Chemistry Authors: Ali M, Bozdag M, Angeli A, Carta F, Capasso C, Farooq U, Supuran CT Tags: ChemMedChem Source Type: research

Safer In Vitro Drug Screening Models for Melioidosis Therapy Development.
Abstract Melioidosis is a neglected tropical disease caused by the Gram-negative soil bacterium Burkholderia pseudomallei. Current antibiotic regimens used to treat melioidosis are prolonged and expensive, and often ineffective because of intrinsic and acquired antimicrobial resistance. Efforts to develop new treatments for melioidosis are limited by the risks associated with handling pathogenic B. pseudomallei, which restricts research to facilities with biosafety level three containment. Closely related nonpathogenic Burkholderia can be investigated under less stringent biosafety level two containment, and we hy...
Source: The American Journal of Tropical Medicine and Hygiene - September 20, 2020 Category: Tropical Medicine Authors: Amiss AS, Webb JR, Mayo M, Currie BJ, Craik DJ, Henriques ST, Lawrence N Tags: Am J Trop Med Hyg Source Type: research

Rhamnolipid enhances Burkholderia pseudomallei biofilm susceptibility, disassembly and production of virulence factors
Future Microbiology,Volume 15, Issue 12, Page 1109-1121, August 2020. (Source: Future Microbiology)
Source: Future Microbiology - September 20, 2020 Category: Microbiology Authors: Jos é JC Sidrim Crister J Ocadaque Bruno R Amando Glaucia M de M Guedes Cec ília L Costa Raimunda SN Brilhante Rossana de A Cordeiro Marcos FG Rocha D ébora SCM Castelo-Branco Source Type: research

Splenic abscess and association with melioidosis in an endemic region: A retrospective review of 39 cases in Kapit, Sarawak, Malaysian Borneo
Background: Splenic abscess is an uncommon condition with an estimated incidence rate of 0.05 –0.7% in autopsy studies. Causative microorganisms can be very diverse. However, in areas where melioidosis is endemic, Burkholderia pseudomallei is the most frequent cause of splenic abscess. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - September 17, 2020 Category: Infectious Diseases Authors: C.Y. Chang, L. Chai, S.C. Huong Tags: 0629 Source Type: research

Predicting the mortality of melioidosis in the heart of Borneo
Background: Melioidosis, caused by Burkholderia pseudomallei, is prevalent in rural areas of Malaysia. This soil-dwelling pathogen is inherently resistant to many first-line antibiotics and carries a high mortality rate. Hence, it poses a significant burden of disease in terms of productivity losses among farmers and blue-collar workers in resource-poor environments. There are limited reports on the factors associated with mortality from melioidosis in Borneo. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - September 17, 2020 Category: Infectious Diseases Authors: V. Toh, S.P. Tee, D. Ang, C.Y. Chang Tags: 0525 Source Type: research

The role of chemoenzymatic synthesis in advancing trehalose analogues as tools for combatting bacterial pathogens.
We describe the role of TreT catalysis and related methods in the development of (i) tools for in vitro and in vivo imaging of mycobacteria, (ii) anti-biofilm compounds that sensitize drug-tolerant mycobacteria to clinical anti-tubercular compounds, and (iii) degradation-resistant trehalose analogues that block trehalose metabolism in C. difficile and potentially other trehalose-utilizing bacteria. We conclude by recapping progress and discussing priorities for future research in this area, including improving the scope and scale of chemoenzymatic synthesis methods to support translational research and expanding the functi...
Source: Chemical Communications - September 10, 2020 Category: Chemistry Authors: Kalera K, Stothard AI, Woodruff PJ, Swarts BM Tags: Chem Commun (Camb) Source Type: research

Case Report: Six New Cases of Melioidosis in the Indian Ocean Region: Comoros, Madagascar, Reunion Island.
lou N Abstract Melioidosis is an emerging disease caused by the environmental Gram-negative bacillus Burkholderia pseudomallei. Melioidosis has been reported to be endemic mainly in northern Australia and Southeast Asia. Here, we report the first two cases of melioidosis on the Comorian island of Mayotte. We also describe four cases that occurred over a short period of time in patients who had traveled between Mayotte, Madagascar, and Reunion Island. PMID: 32876008 [PubMed - as supplied by publisher] (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - August 30, 2020 Category: Tropical Medicine Authors: Renou A, Miltgen G, Blondé R, Jean F, Allyn J, Allou N Tags: Am J Trop Med Hyg Source Type: research

Burkholderia pseudomallei septic arthritis in Type-2 diabetes mellitus patients: Report of two cases
K Baruah Frincy, Borkakoty Biswajyoti, Satyaki Saikia, Manash P Baruah, Utpala DeviIndian Journal of Medical Microbiology 2020 38(2):222-225 Two cases of Burkholderia pseudomallei septic arthritis are presented with a brief review of the literature. B. pseudomallei septic arthritis most commonly occurs in diabetics and other immunocompromised patients and may prove fatal despite appropriate therapy. Clinical and microbiological suspicion of B. pseudomallei infection may help in providing appropriate empirical therapy. (Source: Indian Journal of Medical Microbiology)
Source: Indian Journal of Medical Microbiology - August 28, 2020 Category: Microbiology Authors: K Baruah Frincy Borkakoty Biswajyoti Satyaki Saikia Manash P Baruah Utpala Devi Source Type: research

Detecting the nature and solving the crystal structure of a contaminant protein from an opportunistic pathogen
The unintentional crystallization of contaminant proteins in the place of target recombinant proteins is sporadically reported, despite the availability of stringent expression/purification protocols and of software for the detection of contaminants. Typically, the contaminant protein originates from the expression organism (for example Escherichia coli), but in rare circumstances contaminants from different sources have been reported. Here, a case of contamination from a Serratia bacterial strain that occurred while attempting to crystallize an unrelated protein from Burkholderia pseudomallei (overexpressed in E. coli) is...
Source: Acta Crystallographica Section F - August 27, 2020 Category: Biochemistry Authors: Pederzoli, R. Tarantino, D. Gourlay, L.J. Chaves-Sanjuan, A. Bolognesi, M. Tags: contaminant proteins cyanase hydratase Serratia research communications Source Type: research

Serum From Melioidosis Survivors Diminished Intracellular Burkholderia pseudomallei Growth in Macrophages: A Brief Research Report
Melioidosis is a neglected tropical disease with high mortality rate. It is caused by the Gram-negative, CDC category B select agent Burkholderia pseudomallei (B. ps) that is intrinsically resistant to first-line antibiotics. An antibody-based vaccine is likely to be the most effective control measure. Previous studies have demonstrated significant mechanistic roles of antibodies in protection against death in animal models, but data from human melioidosis is scarce. Herein, we used in-vitro antibody-dependent cellular phagocytosis and growth inhibition assays to assess the mechanism of protective antibodies in patients wi...
Source: Frontiers in cellular and infection microbiology - August 25, 2020 Category: Microbiology Source Type: research

A DUF4148 family protein produced inside RAW264.7 cells is a critical Burkholderia pseudomallei virulence factor
Volume 11, Issue 1, December 2020, Page 1041-1058 . (Source: Virulence)
Source: Virulence - August 23, 2020 Category: Virology Authors: Susan Welkos Irma Blanco Udoka Okaro Jennifer Chua David DeShazer Source Type: research

Jugular Lymphadenitis as a Precursor to Burkholderia Pseudomallei Sepsis
We report a jugular lymphnode abscess associated with facial percutaneous inoculation of B. pseudomallei. Hospital course was complicated by endocarditis, septic arthritis and pyelonephritis. Surgical drainage and intensive and eradicative phase of antibiotics successfully cured the disease. (Source: Journal of Maxillofacial and Oral Surgery)
Source: Journal of Maxillofacial and Oral Surgery - August 12, 2020 Category: ENT & OMF Source Type: research

Burkholderia pseudomallei interferes with host lipid metabolism via NR1D2-mediated PNPLA2/ATGL suppression to block autophagy-dependent inhibition of infection.
This study proposes potential targets for clinical therapy of melioidosis. ABBREVIATIONS: 3-MA: 3-methyladenine; ACTB: actin beta; ATG7: autophagy related 7; B. pseudomallei: Burkholderia pseudomallei; CFU: colony-forming unit; DG: diglyceride; FASN: fatty acid synthase; GFP: green fluorescent protein; LAMP1: lysosomal associated membrane protein 1; LC-MS/MS: liquid chromatography-tandem mass spectrometry; LD: lipid droplet; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MG: monoglyceride; MOI: multiplicity of infection; mRFP: monomeric red fluorescent protein; NR1D2: nuclear receptor subfamily 1 ...
Source: Autophagy - August 10, 2020 Category: Cytology Authors: Tang M, Hu Z, Rao C, Chen J, Yuan S, Zhang J, Mao C, Yan J, Xia Y, Zhang M, Yue J, Xiang Y, Xie J, Mao X, Li Q Tags: Autophagy Source Type: research

NMR fragment screening reveals a novel small molecule binding site near the catalytic surface of the disulfide-dithiol oxidoreductase enzyme DsbA from Burkholderia pseudomallei.
Abstract The presence of suitable cavities or pockets on protein structures is a general criterion for a therapeutic target protein to be classified as 'druggable'. Many disease-related proteins that function solely through protein-protein interactions lack such pockets, making development of inhibitors by traditional small-molecule structure-based design methods much more challenging. The 22 kDa bacterial thiol oxidoreductase enzyme, DsbA, from the gram-negative bacterium Burkholderia pseudomallei (BpsDsbA) is an example of one such target. The crystal structure of oxidized BpsDsbA lacks well-defined surface...
Source: Journal of Bimolecular NMR - August 5, 2020 Category: Biomedical Science Authors: Nebl S, Alwan WS, Williams ML, Sharma G, Taylor A, Doak BC, Wilde KL, McMahon RM, Halili MA, Martin JL, Capuano B, Fenwick RB, Mohanty B, Scanlon MJ Tags: J Biomol NMR Source Type: research

Case Report and Literature Review: Gestational Melioidosis through Perinatal Transmission.
We report the first case of native gestational and neonatal melioidosis in the Western hemisphere. The isolated strains in the mother and newborn were confirmed by whole-genome sequencing and identified as a novel sequence type ST1748. The comparison of both genomes revealed a nucleotide similarity of 100%. Melioidosis should be considered within the differential diagnosis of febrile illness or pneumonia in pregnant women and newborns from endemic areas of the Americas. PMID: 32748772 [PubMed - as supplied by publisher] (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - August 2, 2020 Category: Tropical Medicine Authors: Rodríguez JY, Huertas MG, Rodríguez GJ, Vargas-Otalora S, Benıtez-Peñuela MA, Esquea K, Rios R, Mendoza LR, Diaz L, Reyes J, Álvarez-Moreno CA Tags: Am J Trop Med Hyg Source Type: research

IJERPH, Vol. 17, Pages 5203: Distribution of Serological Response to Burkholderia pseudomallei in Swine from Three Provinces of Vietnam
Conclusions: Initial serological sampling identified a significant number of seropositive and potential melioidosis infections occurring in swine in Vietnam. This work is a critical step in understanding the role swine may play in the epidemiology of human melioidosis in Vietnam. (Source: International Journal of Environmental Research and Public Health)
Source: International Journal of Environmental Research and Public Health - July 17, 2020 Category: Environmental Health Authors: Michael H. Norris Hang Thi Thu Tran Morgan A. Walker Andrew P. Bluhm Diansy Zincke Trinh Thanh Trung Nga Vu Thi Ngoc Pham Thi Herbert P. Schweizer Fred Unger Jason K. Blackburn Nguyen Thi Thu Hang Tags: Article Source Type: research

Melioidosis: A Neglected Cause of Community-Acquired Pneumonia.
Abstract Melioidosis, caused by the facultative intracellular gram-negative pathogen Burkholderia pseudomallei, is an emerging cause of community-acquired pneumonia across the tropics. The majority of patients present with pneumonia with or without sepsis, but localized and asymptomatic infection is also well recognized. Recent modeling and epidemiological studies have demonstrated the widespread presence of B. pseudomallei in otherwise unrecognized regions with a predicted mortality of 90,000 deaths worldwide. Innovative environmental studies are also uncovering how hydrodynamic, pedology, fauna, and weather even...
Source: Respiratory Care - July 8, 2020 Category: Respiratory Medicine Authors: Virk HS, Mukhopadhyay C, Wiersinga WJ Tags: Semin Respir Crit Care Med Source Type: research

Bacterial-induced cell fusion is a danger signal triggering cGAS-STING pathway via micronuclei formation [Microbiology]
Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease in the tropics and subtropics with high morbidity and mortality. The facultative intracellular bacterium induces host cell fusion through its type VI secretion system 5 (T6SS5) as an important part of its pathogenesis in mammalian hosts. This allows it... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - July 6, 2020 Category: Science Authors: Joanne Wei Kay Ku, Yahua Chen, Bryan Jian Wei Lim, Stephan Gasser, Karen Carmelina Crasta, Yunn-Hwen Gan Tags: Biological Sciences Source Type: research

Melioidosis: A Neglected Cause of Community-Acquired Pneumonia
Semin Respir Crit Care Med 2020; 41: 496-508 DOI: 10.1055/s-0040-1710570Melioidosis, caused by the facultative intracellular gram-negative pathogen Burkholderia pseudomallei, is an emerging cause of community-acquired pneumonia across the tropics. The majority of patients present with pneumonia with or without sepsis, but localized and asymptomatic infection is also well recognized. Recent modeling and epidemiological studies have demonstrated the widespread presence of B. pseudomallei in otherwise unrecognized regions with a predicted mortality of 90,000 deaths worldwide. Innovative environmental studies are also uncoveri...
Source: Seminars in Respiratory and Critical Care Medicine - July 5, 2020 Category: Respiratory Medicine Authors: Virk, Harjeet Singh Mukhopadhyay, Chiranjay Wiersinga, W. Joost Tags: Review Article Source Type: research

Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis [Bacteriology]
Melioidosis is caused by Burkholderia pseudomallei and is predominantly seen in tropical regions. The clinical signs and symptoms of the disease are nonspecific and often result in misdiagnosis, failure of treatment, and poor clinical outcome. Septicemia with septic shock is the most common cause of death, with mortality rates above 40%. Bacterial culture is the gold standard for diagnosis, but it has low sensitivity and takes days to produce definitive results. Early laboratory diagnosis can help guide physicians to provide treatment specific to B. pseudomallei. In our study, we adapted host gene expression signatures obt...
Source: Journal of Clinical Microbiology - June 23, 2020 Category: Microbiology Authors: Sangwichian, O., Whistler, T., Nithichanon, A., Kewcharoenwong, C., Sein, M. M., Arayanuphum, C., Chantratita, N., Lertmemongkolchai, G. Tags: Bacteriology Source Type: research

Regulation of Virulence by Two-Component Systems in Pathogenic Burkholderia [Minireviews]
The regulation and timely expression of bacterial genes during infection is critical for a pathogen to cause an infection. Bacteria have multiple mechanisms to regulate gene expression in response to their environment, one of which is two-component systems (TCS). TCS have two components. One component is a sensory histidine kinase (HK) that autophosphorylates when activated by a signal. The activated sensory histidine kinase then transfers the phosphoryl group to the second component, the response regulator, which activates transcription of target genes. The genus Burkholderia contains members that cause human disease and ...
Source: Infection and Immunity - June 21, 2020 Category: Infectious Diseases Authors: Schaefers, M. M. Tags: Minireviews Source Type: research

Talus Osteomyelitis: An Uncommon Manifestation of Melioidosis
The patient was a 56-year-old man with progressive right ankle pain and swelling with no fever or systemic symptoms. He had poorly controlled diabetes mellitus and a remote history of splenic melioidosis 14 years ago. Initial radiographs were unremarkable (Fig. 1A). MRI of the right ankle showed marrow edema and enhancement of the talus (Fig. 1B –D) suspicious for osteomyelitis versus bone marrow edema syndrome. He underwent an open biopsy of the talus and tissue cultures grew Burkholderia pseudomallei. (Source: International Journal of Infectious Diseases)
Source: International Journal of Infectious Diseases - June 2, 2020 Category: Infectious Diseases Authors: Mai Adnan Banjar, Loon Ying Tan, Louise Elizabeth Gartner, James Thomas Patrick Decourcy Hallinan Source Type: research

Point-of-Care Ultrasound in the Diagnosis of Melioidosis in Laos.
Abstract Melioidosis is endemic in many rural areas in Southeast Asia where facilities for culture and identification of Burkholderia pseudomallei are often limited. We performed a prospective observational study in patients presenting with fever to Mahosot Hospital, the primary referral hospital in Laos, to establish whether the detection of abscesses on ultrasound could support a presumptive diagnosis of melioidosis. All patients underwent ultrasound examination to detect abscesses in the liver, spleen, prostate, or, if indicated, subcutaneous tissue. We enrolled 153 patients, including 18 patients with melioido...
Source: Am J Trop Med Hyg - May 31, 2020 Category: Infectious Diseases Authors: Huson MAM, Kling K, Chankongsin S, Phongluxa K, Keoluangkhot V, Newton PN, Dance D, Heller T, Neumayr A Tags: Am J Trop Med Hyg Source Type: research

Polymicrobial community-acquired Acinetobacter baumannii and Burkholderia pseudomallei bacteremia: opportunistic infections with similar risk factors in northern Australia
Publication date: Available online 23 May 2020Source: IDCasesAuthor(s): Lee Fairhead, John Vardanega, Rakesh Pandey, Simon Smith (Source: IDCases)
Source: IDCases - May 24, 2020 Category: Infectious Diseases Source Type: research

In silico prediction of host-pathogen protein interactions in melioidosis pathogen Burkholderia pseudomallei and human reveals novel virulence factors and their targets.
Abstract The aerobic, Gram-negative motile bacillus, Burkholderia pseudomallei is a facultative intracellular bacterium causing melioidosis, a critical disease of public health importance, which is widely endemic in the tropics and subtropical regions of the world. Melioidosis is associated with high case fatality rates in animals and humans; even with treatment, its mortality is 20-50%. It also infects plants and is designated as a biothreat agent. B. pseudomallei is pathogenic due to its ability to invade, resist factors in serum and survive intracellularly. Despite its importance, to date only a few effector pr...
Source: Briefings in Bioinformatics - May 22, 2020 Category: Bioinformatics Authors: Loaiza CD, Duhan N, Lister M, Kaundal R Tags: Brief Bioinform Source Type: research

Extrication of the Microbial Interactions of Activated Sludge Used in the Textile Effluent Treatment of Anaerobic Reactor Through Metagenomic Profiling.
This study is applied to a nanopore sequencing to understand the microbial organisms involved in degradation pathways, and their interaction among them. The taxonomic profile determined the occurrence of Proteobacteria, Actinobacteria, Terrabacteria, bacteria, and Firmicutes with the dominance of Burkholderiales. Burkholderia pseudomallei was found to be 13.79% of the classified reads. The Gene ontology evaluation revealed that mobile elements and tRNA genes were the predominant genetic composition of the community with 16% of the total genes. The functional annotation of genes ensured the presence of genes encoding variou...
Source: Current Microbiology - May 21, 2020 Category: Microbiology Authors: Krishnaswamy VG, Aishwarya S, Kathawala TM Tags: Curr Microbiol Source Type: research

Septic arthritis of knee due to Burkholderia pseudomallei: a case report.
We report such a case with septic arthritis of the knee, diagnosed on the basis of a positive pus culture. After being treated by arthrotomy and surgical debridement followed by a combination of intravenous ceftazidime (acute phase = three weeks) and oral doxycycline and co-trimoxazole (eradication phase = five weeks) for two months, the patient recovered completely with no recurrence. PMID: 32437295 [PubMed - as supplied by publisher] (Source: Tropical Doctor)
Source: Tropical Doctor - May 20, 2020 Category: Tropical Medicine Authors: Parija D, Kar BK, Das P, Mishra JK, Agrawal AC, Yadav SK Tags: Trop Doct Source Type: research

Molecules, Vol. 25, Pages 2269: Crystal Structure of a Tetrameric Type II β-Carbonic Anhydrase from the Pathogenic Bacterium Burkholderia pseudomallei
Molecules, Vol. 25, Pages 2269: Crystal Structure of a Tetrameric Type II β-Carbonic Anhydrase from the Pathogenic Bacterium Burkholderia pseudomallei Molecules doi: 10.3390/molecules25102269 Authors: Andrea Angeli Marta Ferraroni Mariana Pinteala Stelian S. Maier Bogdan C. Simionescu Fabrizio Carta Sonia Del Prete Clemente Capasso Claudiu T. Supuran Carbonic anhydrase (CA) is a zinc enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate and proton. Currently, CA inhibitors are widely used as antiglaucoma, anticancer, and anti-obesity drugs and for the treatment of neu...
Source: Molecules - May 11, 2020 Category: Chemistry Authors: Andrea Angeli Marta Ferraroni Mariana Pinteala Stelian S. Maier Bogdan C. Simionescu Fabrizio Carta Sonia Del Prete Clemente Capasso Claudiu T. Supuran Tags: Article Source Type: research

Molecular Characteristics of Burkholderia pseudomallei Collected From Humans in Hainan, China
In this study, multilocus variable-number tandem repeat analysis (MLVA), and multilocus sequence typing (MLST) were applied to examine the epidemiological relatedness and population structure of 166 B. pseudomallei isolates obtained during 2002–2014 in Hainan, China. Both the MLVA_4 and MLST approaches had high discriminatory power for this population, with diversity indices of 0.9899 and 0.9457, respectively. However, the MLVA_4 assay showed a higher discriminatory power than the MLST approach, and a variable-number tandem repeat (VNTR3 933) found by the MLVA approach was the most useful in discriminating strains fr...
Source: Frontiers in Microbiology - May 6, 2020 Category: Microbiology Source Type: research

A comparative genomic analysis of small-colony variant and wild-type Burkholderia pseudomallei in a patient with bacterial liver abscess
Publication date: June 2020Source: Journal of Global Antimicrobial Resistance, Volume 21Author(s): Xuming Wang, Xiao Zheng, Meihui Huang, Lingli Liu (Source: Journal of Global Antimicrobial Resistance)
Source: Journal of Global Antimicrobial Resistance - April 30, 2020 Category: Infectious Diseases Source Type: research

Is GSK3 β a molecular target of chloroquine treatment against COVID-19?
Is GSK3β a molecular target of chloroquine treatment against COVID-19? Drug Discov Ther. 2020 Apr 21;: Authors: Embi MN, Ganesan N, Sidek HM Abstract The recent clinical trial reports pertaining to the efficacy of chloroquine and hydroxychloroquine against COVID-19 albeit yet to be validated with larger clinical trials, have sparked much interest globally to evaluate whether this anti-malarial drug can be repurposed for the treatment of COVID-19. In addition to its anti-viral activity, the anti-inflammatory activity of chloroquine may also contribute to its efficacy. Based on our data obtained fro...
Source: Drug Discoveries and Therapeutics - April 25, 2020 Category: Drugs & Pharmacology Tags: Drug Discov Ther Source Type: research

Burkholderia pseudomallei was Identified in a Melioidosis Aneurysm using Polymerase Chain Reaction Targeting 23S rRNA
We describe an attempt to identify Burkholderia pseudomallei immediately, which was confirmed by polymerase chain reaction (PCR) and gene sequence analysis of 23S rRNA gene. PCR is not only an unambiguous identification of B. pseudomallei but also a rapid detection because B. pseudomallei may not be readily isolated. For patients of melioidosis abdominal aortic aneurysm with spleen abscess, prolonged antibiotic therapy, splenectomy and artificial vessel replacement provided an excellent result in our study. (Source: Annals of Vascular Surgery)
Source: Annals of Vascular Surgery - April 23, 2020 Category: Surgery Authors: Li Shi, Jie Chen, Gao Yi Tags: Case Report Source Type: research

Virulence of the emerging pathogen, Burkholderia pseudomallei, depends upon the O-linked oligosaccharyltransferase, PglL
Future Microbiology,Volume 15, Issue 4, Page 241-257, March 2020. (Source: Future Microbiology)
Source: Future Microbiology - April 8, 2020 Category: Microbiology Authors: Samuel J Willcocks Carmen Denman Felipe Cia Elizabeth McCarthy Jon Cuccui Brendan W Wren Source Type: research

Survival of Burkholderia pseudomallei and Pathogenic Leptospira in Cola, Beer, Energy Drinks, and Sports Drinks.
In conclusion, Leptospira can survive in some beverages for up to 15 minutes, whereas B. pseudomallei can survive in some beverages for up to 4 weeks. PMID: 32274989 [PubMed - as supplied by publisher] (Source: Am J Trop Med Hyg)
Source: Am J Trop Med Hyg - April 5, 2020 Category: Infectious Diseases Authors: Wuthiekanun V, Amornchai P, Langla S, White NJ, Day NPJ, Limmathurotsakul D Tags: Am J Trop Med Hyg Source Type: research

Community-acquired bacterial pneumonia in adults: An update.
Abstract Community-acquired pneumonia (CAP) is the prominent cause of mortality and morbidity with important clinical impact across the globe. India accounts for 23 per cent of global pneumonia burden with case fatality rates between 14 and 30 per cent, and Streptococcus pneumoniae is considered a major bacterial aetiology. Emerging pathogens like Burkholderia pseudomallei is increasingly recognized as an important cause of CAP in Southeast Asian countries. Initial management in the primary care depends on clinical assessment while the hospitalized patients require combinations of clinical scores, chest radiograph...
Source: The Indian Journal of Medical Research - March 31, 2020 Category: Biomedical Science Authors: Eshwara VK, Mukhopadhyay C, Rello J Tags: Indian J Med Res Source Type: research

Burkholderia pseudomallei peri-prosthetic infection following medial malleolar internal fixation: a case report
Melioidosis-associated peri-prosthetic infection is extremely rare. To date, melioidosis associated septic arthritis of the ankle joint following a medial malleolar internal fixation has not been reported. (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - March 19, 2020 Category: Infectious Diseases Authors: Umesh Jayarajah, Arulprashanth Arulanantham, Vimaleswaran Koculen, Chamikara Palkumbura, Aadil Faleel and Rukshan Sooriyarachchi Tags: Case report Source Type: research

Human Melioidosis.
Abstract SUMMARYThe causative agent of melioidosis, Burkholderia pseudomallei, a tier 1 select agent, is endemic in Southeast Asia and northern Australia, with increased incidence associated with high levels of rainfall. Increasing reports of this condition have occurred worldwide, with estimates of up to 165,000 cases and 89,000 deaths per year. The ecological niche of the organism has yet to be clearly defined, although the organism is associated with soil and water. The culture of appropriate clinical material remains the mainstay of laboratory diagnosis. Identification is best done by phenotypic methods, altho...
Source: Clinical Microbiology Reviews - March 13, 2020 Category: Microbiology Authors: Gassiep I, Armstrong M, Norton R Tags: Clin Microbiol Rev Source Type: research

Burkholderia pseudomallei pathogenesis and survival in different niches.
Abstract Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease of the tropics with high clinical mortality rates. To date, no vaccines are approved for melioidosis and current treatment relies on antibiotics. Conversely, common misdiagnosis and high pathogenicity of Bp hamper efforts to fight melioidosis. This bacterium can be isolated from a wide range of niches such as waterlogged fields, stagnant water bodies, salt water bodies and from human and animal clinical specimens. Although extensive studies have been undertaken to elucidate pathogenesis mechanisms of Bp, little is known about ...
Source: Biochemical Society Transactions - March 12, 2020 Category: Biochemistry Authors: Yip CH, Ghazali AK, Nathan S Tags: Biochem Soc Trans Source Type: research

Identification of Burkholderia pseudomallei Genes Induced During Infection of Macrophages by Differential Fluorescence Induction
Burkholderia pseudomallei, the causative agent of melioidosis, can survive and replicate in macrophages. Little is known about B. pseudomallei genes that are induced during macrophage infection. We constructed a B. pseudomallei K96243 promoter trap library with genomic DNA fragments fused to the 5′ end of a plasmid-borne gene encoding enhanced green fluorescent protein (eGFP). Microarray analysis showed that the library spanned 88% of the B. pseudomallei genome. The recombinant plasmids were introduced into Burkholderia thailandensis E264, and promoter fusions active during in vitro culture were removed. J774A.1 muri...
Source: Frontiers in Microbiology - February 20, 2020 Category: Microbiology Source Type: research

Caspase-4 Mediates Restriction of Burkholderia pseudomallei in Human Alveolar Epithelial Cells [Cellular Microbiology: Pathogen-Host Cell Molecular Interactions]
Melioidosis is an infectious disease with a high mortality rate responsible for community-acquired sepsis in Southeast Asia and Northern Australia. The causative agent of this disease is Burkholderia pseudomallei, a Gram-negative bacterium that resides in soil and contaminated natural water. After entering into host cells, the bacteria escape into the cytoplasm, which has numerous cytosolic sensors, including the noncanonical inflammatory caspases. Although the noncanonical inflammasome (caspase-11) has been investigated in a murine model of B. pseudomallei infection, its role in humans, particularly in lung epithelial cel...
Source: Infection and Immunity - February 19, 2020 Category: Infectious Diseases Authors: Srisaowakarn, C., Pudla, M., Ponpuak, M., Utaisincharoen, P. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research