Copulation of < em > Ixodes ricinus < /em > males and females on the host and its potential impact on pathogen transmission
CONCLUSIONS: By increasing the feeding dynamics in females, the copulation of I. ricinus males with females attached to the host's skin may stimulate pathogen replication in tick tissues and migration from the gut to the salivary glands. Further investigations of the I. ricinus copulation on the host on female feeding and pathogen transmission may contribute to the elucidation of the eco-epidemiology of tick-borne diseases transmitted by this tick species.PMID:38153063 | DOI:10.26444/aaem/174105 (Source: Annals of Agricultural and Environmental Medicine : AAEM)
Source: Annals of Agricultural and Environmental Medicine : AAEM - December 28, 2023 Category: Environmental Health Authors: Weronika Buczek Alicja Buczek Marek Asman Katarzyna Bartosik Source Type: research

Copulation of < em > Ixodes ricinus < /em > males and females on the host and its potential impact on pathogen transmission
CONCLUSIONS: By increasing the feeding dynamics in females, the copulation of I. ricinus males with females attached to the host's skin may stimulate pathogen replication in tick tissues and migration from the gut to the salivary glands. Further investigations of the I. ricinus copulation on the host on female feeding and pathogen transmission may contribute to the elucidation of the eco-epidemiology of tick-borne diseases transmitted by this tick species.PMID:38153063 | DOI:10.26444/aaem/174105 (Source: Annals of Agricultural and Environmental Medicine : AAEM)
Source: Annals of Agricultural and Environmental Medicine : AAEM - December 28, 2023 Category: Environmental Health Authors: Weronika Buczek Alicja Buczek Marek Asman Katarzyna Bartosik Source Type: research

Editorial: Babesia: biology, interactions, and mechanisms of pathogenesis in ticks and its hosts volume II
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - December 20, 2023 Category: Microbiology Source Type: research

Detection and quantification of Babesia species intraerythrocytic parasites by flow cytometry
CONCLUSIONS: This investigation demonstrates that Babesia parasites can be detected and quantified directly from venous blood using FLC. Although promising, opportunities remain to improve the general applicability of the method.PMID:38113371 | DOI:10.1093/ajcp/aqad168 (Source: American Journal of Clinical Pathology)
Source: American Journal of Clinical Pathology - December 19, 2023 Category: Pathology Authors: Patrick M Vanderboom Anisha Misra Kyle G Rodino Allison R Eberly Jason D Greenwood Heather E Morris Felicity C Norrie Emily C Fernholz Bobbi S Pritt Andrew P Norgan Source Type: research

Detection and quantification of Babesia species intraerythrocytic parasites by flow cytometry
CONCLUSIONS: This investigation demonstrates that Babesia parasites can be detected and quantified directly from venous blood using FLC. Although promising, opportunities remain to improve the general applicability of the method.PMID:38113371 | DOI:10.1093/ajcp/aqad168 (Source: American Journal of Clinical Pathology)
Source: American Journal of Clinical Pathology - December 19, 2023 Category: Pathology Authors: Patrick M Vanderboom Anisha Misra Kyle G Rodino Allison R Eberly Jason D Greenwood Heather E Morris Felicity C Norrie Emily C Fernholz Bobbi S Pritt Andrew P Norgan Source Type: research

Babesia divergens
Trends Parasitol. 2023 Dec 15:S1471-4922(23)00288-X. doi: 10.1016/j.pt.2023.11.008. Online ahead of print.NO ABSTRACTPMID:38104025 | DOI:10.1016/j.pt.2023.11.008 (Source: Trends in Parasitology)
Source: Trends in Parasitology - December 16, 2023 Category: Parasitology Authors: Ana Maria Filipe Viktoriya Levytska Marie Jalovecka Source Type: research

Babesia divergens
Trends Parasitol. 2023 Dec 15:S1471-4922(23)00288-X. doi: 10.1016/j.pt.2023.11.008. Online ahead of print.NO ABSTRACTPMID:38104025 | DOI:10.1016/j.pt.2023.11.008 (Source: Trends in Parasitology)
Source: Trends in Parasitology - December 16, 2023 Category: Parasitology Authors: Ana Maria Filipe Viktoriya Levytska Marie Jalovecka Source Type: research

Babesia divergens
Trends Parasitol. 2023 Dec 15:S1471-4922(23)00288-X. doi: 10.1016/j.pt.2023.11.008. Online ahead of print.NO ABSTRACTPMID:38104025 | DOI:10.1016/j.pt.2023.11.008 (Source: Trends in Parasitology)
Source: Trends in Parasitology - December 16, 2023 Category: Parasitology Authors: Ana Maria Filipe Viktoriya Levytska Marie Jalovecka Source Type: research

Babesia divergens
Trends Parasitol. 2023 Dec 15:S1471-4922(23)00288-X. doi: 10.1016/j.pt.2023.11.008. Online ahead of print.NO ABSTRACTPMID:38104025 | DOI:10.1016/j.pt.2023.11.008 (Source: Trends in Parasitology)
Source: Trends in Parasitology - December 16, 2023 Category: Parasitology Authors: Ana Maria Filipe Viktoriya Levytska Marie Jalovecka Source Type: research

Establishment of a stable transfection and gene targeting system in Babesia divergens
In this study, we address the current limitation of functional genomic tools in B. divergens and introduce a stable transfection system specific to this parasite. We define the parameters for a drug selection system hdhfr-WR99210 and evaluate different transfection protocols for highly efficient generation of transgenic parasites expressing GFP. We proved that plasmid delivery into bovine erythrocytes prior to their infection is the most optimal transfection approach for B. divergens, providing novel evidence of Babesia parasites’ ability to spontaneously uptake external DNA from erythrocytes cytoplasm. Furthermore, we v...
Source: Frontiers in cellular and infection microbiology - December 13, 2023 Category: Microbiology Source Type: research

Wide bovine tick-borne pathogen spectrum: Predominancy of Theileria annulata and the first molecular detection of Ehrlichia minasensis in Turkey
This study aimed to investigate significant bovine tick-borne piroplasm, rickettsia, and some other bacterial agents by genus- or species-specific PCR and nested PCR techniques in Turkey. A total of 210 cattle blood samples were collected from sixteen provinces in different geographical regions of Turkey. PCR analyses were performed targeting the detection ofBabesia/Theileria/Hepatozoon sp. 18S rRNA,Babesia/Theileria sp. 18S rRNA (V4),B. bigemina RAP-1a,B. bovis SBP-4,B. ovata AMA-1,B. naoaki AMA-1,T. annulata Tams-1,T. orientalis MPSP,T. mutans 18S rRNA,Anaplasma/Ehrlichia sp.16S rRNA,A. marginale MSP4,A. bovis 16S rRNA,A...
Source: Veterinary Research Communications - December 11, 2023 Category: Veterinary Research Source Type: research

Hematological profile of healthy Thoroughbred foals from birth to one month of age in Trinidad, West Indies
This study aimed to develop a sound database for the hematological reference intervals of thoroughbred foals in Trinidad, West Indies from birth to 1 month of age.ANIMALS: 89 foals.METHODS: Whole blood samples were taken from 89 foals throughout Trinidad at approximately 1 day, 1 week, and 1 month of age. These foals were examined to be classified as healthy or free from disease. Complete blood count (CBC), microscopic analysis of blood smears, and conventional PCR for Theileria equi and Babesia caballi were performed.RESULTS: Of the 89 foals, 67 were deemed healthy and suitable for establishing reference intervals. Foals ...
Source: Cell Research - November 29, 2023 Category: Cytology Authors: Candice Sant Daniel M Lima Roger d'Abadie Indira Pargass Karla C Georges Source Type: research

Hematological profile of healthy Thoroughbred foals from birth to one month of age in Trinidad, West Indies
This study aimed to develop a sound database for the hematological reference intervals of thoroughbred foals in Trinidad, West Indies from birth to 1 month of age.ANIMALS: 89 foals.METHODS: Whole blood samples were taken from 89 foals throughout Trinidad at approximately 1 day, 1 week, and 1 month of age. These foals were examined to be classified as healthy or free from disease. Complete blood count (CBC), microscopic analysis of blood smears, and conventional PCR for Theileria equi and Babesia caballi were performed.RESULTS: Of the 89 foals, 67 were deemed healthy and suitable for establishing reference intervals. Foals ...
Source: American Journal of Veterinary Research - November 29, 2023 Category: Veterinary Research Authors: Candice Sant Daniel M Lima Roger d'Abadie Indira Pargass Karla C Georges Source Type: research

Detection of a novel Babesia sp. in Amblyomma javanense, an ectoparasite of Sunda pangolins
Babesia is a protozoal, tick-borne parasite that can cause life-threatening disease in humans, wildlife and domestic animals worldwide. However, in Southeast Asia, little is known about the prevalence and diversi... (Source: Parasites and Vectors)
Source: Parasites and Vectors - November 22, 2023 Category: Microbiology Authors: Stacy Q. Y. Chong, Darren Yeo, Nur Insyirah Aidil, Jasmine L. Y. Ong, Amy H. J. Chan, Charlene Judith Fernandez, Bryan T. M. Lim, Max D. Y. Khoo, Anna M. S. Wong, Siow Foong Chang and Him Hoo Yap Tags: Brief Report Source Type: research

Neurologic Manifestations of Tick-Borne Diseases Transmitted by Deer Ticks (Ixodes scapularis) in the USA
This article aims to review the relevant updates in pathogenesis, diagnostics, clinical manifestations, and treatments of tick-borne diseases involving the nervous system, with special emphasis on emerging viral and bacterial infections transmitted by deer ticks (Ixodes scapularis).Recent Findings Neuroborreliosis includes a wide array of peripheral and/or central nervous system syndromes, of which diagnosis depends on a combination of clinical gestalt, serum serologic testing, and CSF cellular and serologic analysis. Powassan virus may cause life-threatening neuroinvasive disease and diagnosis that is based on CSF serolog...
Source: Current Tropical Medicine Reports - November 17, 2023 Category: Tropical Medicine Source Type: research