Assessment of Babesia spp. prevalence in various domestic animals across Southern Punjab, Pakistan
This study, conducted in Southern Punjab, Pakistan, aimed to assess the prevalence of Babesia spp. across various livestock species using microscopic and PCR methods. A total of 180 blood samples (60 from each district) were systematically collected from apparently healthy animals, with 36 samples obtained from each domestic animal species, including camel, cattle, buffalo, goat, and sheep, noting that 12 samples were collected from each district for each animal species. Overall prevalence was determined to be 32.8% (59/180), with varying rates among species: 25.0% in cattle, 41.66% in buffalo, 30.55% in goats, 33.3% in sh...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M Tariq F Badshah N U Khan M I Zafar P R De Los R íos-Escalante E Ib áñez-Arancibia S Mehnaz M Mubashir M S Khan M S Khan N Rafiq N Shaheen S Saeed M Ben Said Source Type: research

Assessment of Babesia spp. prevalence in various domestic animals across Southern Punjab, Pakistan
This study, conducted in Southern Punjab, Pakistan, aimed to assess the prevalence of Babesia spp. across various livestock species using microscopic and PCR methods. A total of 180 blood samples (60 from each district) were systematically collected from apparently healthy animals, with 36 samples obtained from each domestic animal species, including camel, cattle, buffalo, goat, and sheep, noting that 12 samples were collected from each district for each animal species. Overall prevalence was determined to be 32.8% (59/180), with varying rates among species: 25.0% in cattle, 41.66% in buffalo, 30.55% in goats, 33.3% in sh...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M Tariq F Badshah N U Khan M I Zafar P R De Los R íos-Escalante E Ib áñez-Arancibia S Mehnaz M Mubashir M S Khan M S Khan N Rafiq N Shaheen S Saeed M Ben Said Source Type: research

Assessment of Babesia spp. prevalence in various domestic animals across Southern Punjab, Pakistan
This study, conducted in Southern Punjab, Pakistan, aimed to assess the prevalence of Babesia spp. across various livestock species using microscopic and PCR methods. A total of 180 blood samples (60 from each district) were systematically collected from apparently healthy animals, with 36 samples obtained from each domestic animal species, including camel, cattle, buffalo, goat, and sheep, noting that 12 samples were collected from each district for each animal species. Overall prevalence was determined to be 32.8% (59/180), with varying rates among species: 25.0% in cattle, 41.66% in buffalo, 30.55% in goats, 33.3% in sh...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M Tariq F Badshah N U Khan M I Zafar P R De Los R íos-Escalante E Ib áñez-Arancibia S Mehnaz M Mubashir M S Khan M S Khan N Rafiq N Shaheen S Saeed M Ben Said Source Type: research

Assessment of Babesia spp. prevalence in various domestic animals across Southern Punjab, Pakistan
This study, conducted in Southern Punjab, Pakistan, aimed to assess the prevalence of Babesia spp. across various livestock species using microscopic and PCR methods. A total of 180 blood samples (60 from each district) were systematically collected from apparently healthy animals, with 36 samples obtained from each domestic animal species, including camel, cattle, buffalo, goat, and sheep, noting that 12 samples were collected from each district for each animal species. Overall prevalence was determined to be 32.8% (59/180), with varying rates among species: 25.0% in cattle, 41.66% in buffalo, 30.55% in goats, 33.3% in sh...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M Tariq F Badshah N U Khan M I Zafar P R De Los R íos-Escalante E Ib áñez-Arancibia S Mehnaz M Mubashir M S Khan M S Khan N Rafiq N Shaheen S Saeed M Ben Said Source Type: research

Assessment of Babesia spp. prevalence in various domestic animals across Southern Punjab, Pakistan
This study, conducted in Southern Punjab, Pakistan, aimed to assess the prevalence of Babesia spp. across various livestock species using microscopic and PCR methods. A total of 180 blood samples (60 from each district) were systematically collected from apparently healthy animals, with 36 samples obtained from each domestic animal species, including camel, cattle, buffalo, goat, and sheep, noting that 12 samples were collected from each district for each animal species. Overall prevalence was determined to be 32.8% (59/180), with varying rates among species: 25.0% in cattle, 41.66% in buffalo, 30.55% in goats, 33.3% in sh...
Source: Braz J Biol - February 29, 2024 Category: Biology Authors: M Tariq F Badshah N U Khan M I Zafar P R De Los R íos-Escalante E Ib áñez-Arancibia S Mehnaz M Mubashir M S Khan M S Khan N Rafiq N Shaheen S Saeed M Ben Said Source Type: research

Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead
CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.PMID:38409720 | DOI:10.2174/0113892010268142231226115140 (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 27, 2024 Category: Biotechnology Authors: Henrique Bonif ácio Moura Henrique Gama Ker Luana S Ramos Lais M Nogueira Juliana M Machado Daniel S Dias Rita Ala íde Leandro Rodrigues Mariana Campos da Paz Rafael Gon çalves Teixeira-Neto Alexandre Barbosa Reis Alexsandro S Galdino Eduardo S Silva Source Type: research

Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead
CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.PMID:38409720 | DOI:10.2174/0113892010268142231226115140 (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 27, 2024 Category: Biotechnology Authors: Henrique Bonif ácio Moura Henrique Gama Ker Luana S Ramos Lais M Nogueira Juliana M Machado Daniel S Dias Rita Ala íde Leandro Rodrigues Mariana Campos da Paz Rafael Gon çalves Teixeira-Neto Alexandre Barbosa Reis Alexsandro S Galdino Eduardo S Silva Source Type: research

Transboundary Tick and Tick-Borne Pathogen Threats to Cattle
Transboundary incursions of ticks and tick-borne pathogens are ever present concerns for US cattle industries. Global trade in livestock and wildlife, historic and emerging transboundary issues with endemic tick populations and pathogens, and migratory bird flyways are pathways of concern. Transboundary challenges are presented for the Asian long-horned tick and Theileria orientalis Ikeda, for 2 cattle fever tick species [Rhipicephalus (Boophilus) annulatus and R (B) microplus] and Babesia bigemina and B bovis, and for the tropical bont tick and Ehrlichia ruminantium. (Source: Veterinary Clinics of North America: Food Animal Practice)
Source: Veterinary Clinics of North America: Food Animal Practice - February 23, 2024 Category: Veterinary Research Authors: Pete D. Teel, Thomas Hairgrove Source Type: research

Parasites and microorganisms associated with the snakes collected for the “ < i > festa Dei serpari < /i > ” in Cocullo, Italy
by Jairo Alfonso Mendoza-Roldan, Livia Perles, Ernesto Filippi, Nicole Szafranski, Gianpaolo Montinaro, Mariaelisa Carbonara, Riccardo Scalera, Pedro Paulo de Abreu Teles, Julia Walochnik, Domenico Otranto While in much of the Western world snakes are feared, in the small, rural, mountainous town of Cocullo, in the middle of central Italy, snakes are annually collected and celebrated in a sacro-profane ritual. Every 1st of May,Serpari (snake catchers) capture and showcase dozens of non-venomous snakes to celebrate the ritual ofSan Domenico. In order to detect potential zoonotic pathogens within this unique epidemiological...
Source: PLoS Neglected Tropical Diseases - February 21, 2024 Category: Tropical Medicine Authors: Jairo Alfonso Mendoza-Roldan Source Type: research

Expression of sex-specific molecular markers by Babesia bovis gametes
Bovine babesiosis caused by Babesia bovis is one of the most important tick-borne diseases of cattle in tropical and subtropical regions. Babesia bovis parasites have a complex lifecycle, including development wi... (Source: Parasites and Vectors)
Source: Parasites and Vectors - February 19, 2024 Category: Microbiology Authors: Hala E. Hussein, Wendell C. Johnson, Naomi S. Taus and Massaro W. Ueti Tags: Research Source Type: research

Epidemiology and genetic diversity of Theileria equi and Babesia caballi in Mongolian horses
Infect Genet Evol. 2024 Feb 14:105571. doi: 10.1016/j.meegid.2024.105571. Online ahead of print.ABSTRACTEquine piroplasmosis is a tick-borne disease caused by Theileria equi and Babesia caballi in horses. Because of its impact on horse industry, control of this disease is crucial for endemic countries. The control of equine piroplasmosis may be influenced by the genotypic diversity of T. equi and B. caballi. Mongolia, a country with a thriving livestock industry, is endemic for T. equi and B. caballi. However, nationwide epidemiological surveys have not been conducted to determine the current status of infections and genet...
Source: Infection, Genetics and Evolution - February 16, 2024 Category: Genetics & Stem Cells Authors: Davaajav Otgonsuren Tovuu Amgalanbaatar Sandagdorj Narantsatsral Batsaikhan Enkhtaivan Dalantai Munkhgerel Myagmar Zoljargal Batbold Davkharbayar Punsantsogvoo Myagmarsuren Banzrach Battur Badgar Battsetseg Thillaiampalam Sivakumar Naoaki Yokoyama Source Type: research

Epidemiology and genetic diversity of Theileria equi and Babesia caballi in Mongolian horses
Infect Genet Evol. 2024 Feb 15;119:105571. doi: 10.1016/j.meegid.2024.105571. Online ahead of print.ABSTRACTEquine piroplasmosis is a tick-borne disease caused by Theileria equi and Babesia caballi in horses. Because of its impact on horse industry, control of this disease is crucial for endemic countries. The control of equine piroplasmosis may be influenced by the genotypic diversity of T. equi and B. caballi. Mongolia, a country with a thriving livestock industry, is endemic for T. equi and B. caballi. However, nationwide epidemiological surveys have not been conducted to determine the current status of infections and g...
Source: Infection, Genetics and Evolution - February 16, 2024 Category: Genetics & Stem Cells Authors: Davaajav Otgonsuren Tovuu Amgalanbaatar Sandagdorj Narantsatsral Batsaikhan Enkhtaivan Dalantai Munkhgerel Myagmar Zoljargal Batbold Davkharbayar Punsantsogvoo Myagmarsuren Banzrach Battur Badgar Battsetseg Thillaiampalam Sivakumar Naoaki Yokoyama Source Type: research

Mixed-sex clusters on grass blades: breeding strategy of the ornate dog tick, Dermacentor reticulatus
The ornate dog tick Dermacentor reticulatus is second only to the hard tick Ixodes ricinus in terms of importance as a vector of infectious organisms, especially of Babesia canis, the agent of canine babesiosis. ... (Source: Parasites and Vectors)
Source: Parasites and Vectors - February 9, 2024 Category: Microbiology Authors: Dagmara W ężyk, Wiktoria Romanek, Wiktoria Małaszewicz, Jerzy M. Behnke and Anna Bajer Tags: Research Source Type: research

Genes, Vol. 15, Pages 215: Exploring the Impact of Primer & ndash;Template Mismatches on PCR Performance of DNA Polymerases Varying in Proofreading Activity
In this study, we strategically designed 111 primer&amp;ndash;template combinations with varying numbers, types, and locations of mismatches to meticulously assess their impact on qPCR performance while two distinctly different types of DNA polymerases were used. Notably, when a single-nucleotide mismatch occurred at the 3&amp;rsquo; end of the primer, we observed significant decreases in the analytical sensitivity (0&amp;ndash;4%) with Invitrogen&amp;trade; Platinum&amp;trade; Taq DNA Polymerase High Fidelity, while the analytical sensitivity remained unchanged with Takara Ex Taq Hot Start Version DNA ...
Source: Genes - February 7, 2024 Category: Genetics & Stem Cells Authors: Ke Huang Jilei Zhang Jing Li Haixiang Qiu Lanjing Wei Yi Yang Chengming Wang Tags: Article Source Type: research

Transmission risk evaluation of transfusion blood containing low-density Babesia microti
ConclusionWhole blood containing extremely low density of B. microti poses a high transmission risk when transfused between mice and mice or human and mice, suggesting that Babesia detection should be considered by governments, hospitals, and disease prevention and control centers as a mandatory test before blood donation or transfusion. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - February 5, 2024 Category: Microbiology Source Type: research