Smart antimicrobial system based on enzyme-responsive high methoxyl pectin-whey protein isolate nanocomplex for fresh-cut apple preservation
This study introduces a novel intelligent preservation strategy for storing fresh-cut apples.PMID:37748593 | DOI:10.1016/j.ijbiomac.2023.127064 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - September 25, 2023 Category: Biochemistry Authors: Ying Xin Zhenzhen Liu Chenhao Yang Chen Dong Fusheng Chen Kunlun Liu Source Type: research

Label-free Raman microspectroscopic imaging with chemometrics for cellular investigation of apple ring rot and nondestructive early recognition using near-infrared reflection spectroscopy with machine learning
Talanta. 2023 Sep 17;267:125212. doi: 10.1016/j.talanta.2023.125212. Online ahead of print.ABSTRACTApple ring rot caused by Botryosphaeria dothidea can cause fruit decay during the growth and storage stages of apple fruit. Understanding the infection process and cellular defense response at the cellular micro-level holds immense importance in the field of prevention and control. Consequently, there is a pressing need to develop suitable chemical imaging analysis methods. Here we proposed a label-free, high-throughput imaging method for cellular investigation of apple fruit ring rot infected by Botryosphaeria dothidea, base...
Source: Talanta - September 23, 2023 Category: Chemistry Authors: Mei Li Lu Zhang Ling-Li Jiang Zhi-Bo Zhao You-Hua Long Dong-Mei Chen Jun Bin Chao Kang Ya-Juan Liu Source Type: research

Physicians as citizens and the indispensability of civic virtues for professional practice
Incivility poses a serious threat to any healthcare system striving for effectiveness without sacrificing the requirements of humanity. Threats to civility within healthcare not only come from individual ‘bad apples’ exhibiting borderline and inacceptable behaviour, as seen in many ‘high-tech, high-risk, high-responsibility’ environments such as operating or emergency rooms.1 They may also be facilitated by ‘bad trees’ or system-immanent, poor healthcare environments.2 This may be the case when healthcare administrations, facing the challenges of political austerity, set budgetary target...
Source: Journal of Medical Ethics - September 22, 2023 Category: Medical Ethics Authors: Monteverde, S. Tags: Commentary Source Type: research

The role of fruits as reservoirs for resistant and virulent strains of opportunistic yeasts
AbstractStrains of theCandida parapsilosis andMeyerozyma guilliermondii species complexes isolated from the internal tissues of 26 fruit species from 21 countries were evaluated for their susceptibility to conventional antifungal compounds (fluconazole, voriconazole, amphotericin B) and hydrolytic activity. A total of 144 strains were studied. Resistance to at least one of the antifungal compounds tested was found in 26.4% of the endophytic strains examined. Most of the strains were insensitive to fluconazole. Multiresistance was detected only in two strains ofC. parapsilosis sensu stricto from tropical apples. Phospholipa...
Source: World Journal of Microbiology and Biotechnology - September 21, 2023 Category: Microbiology Source Type: research

Analyzing Bioaccessibility of Polyphenols in Six Commercial and Six Traditional Apples (Malus domestica Borkh.) during In Vitro and Ex Vivo Oral Digestion
ConclusionDue to higher phenolic contents in combination with a similar release, increased PP concentrations in the oral phase and an improved uptake of PP from traditional varieties are assumed. However, the proposed beneficial effect of phenolics on allergenicity cannot be confirmed. (Source: Molecular Nutrition and Food Research)
Source: Molecular Nutrition and Food Research - September 20, 2023 Category: Food Science Authors: Julia Anna Helene Kaeswurm, Rebecca Sempio, Federica Manca, Melanie Regina Burandt, Maria Buchweitz Tags: Research Article Source Type: research

Feasibility and acceptability of miracle fruit application prior to the consumption of sour-tasting foods as a weight-loss strategy in adults with diabetes or prediabetes: A randomized crossover trial
Appetite. 2023 Sep 17:107046. doi: 10.1016/j.appet.2023.107046. Online ahead of print.ABSTRACTMiracle fruit (MF) has emerged as a promising option for healthy noncaloric sweeteners due to its sour-to-sweet taste-modifying effects and high antioxidant activity. We aimed to examine the feasibility and acceptability of using MF as a weight-loss strategy for individuals with diabetes or prediabetes. Fifty Korean-American men and women (25 participants each) aged 45-75 years with diabetes or prediabetes were recruited in this study. They participated in a randomized placebo-controlled crossover trial where they took the assigne...
Source: Appetite - September 19, 2023 Category: Nutrition Authors: Sung Eun Choi Taehwan Park Source Type: research

Feasibility and acceptability of miracle fruit application prior to the consumption of sour-tasting foods as a weight-loss strategy in adults with diabetes or prediabetes: A randomized crossover trial
Appetite. 2023 Sep 17:107046. doi: 10.1016/j.appet.2023.107046. Online ahead of print.ABSTRACTMiracle fruit (MF) has emerged as a promising option for healthy noncaloric sweeteners due to its sour-to-sweet taste-modifying effects and high antioxidant activity. We aimed to examine the feasibility and acceptability of using MF as a weight-loss strategy for individuals with diabetes or prediabetes. Fifty Korean-American men and women (25 participants each) aged 45-75 years with diabetes or prediabetes were recruited in this study. They participated in a randomized placebo-controlled crossover trial where they took the assigne...
Source: Appetite - September 19, 2023 Category: Nutrition Authors: Sung Eun Choi Taehwan Park Source Type: research

Investigation on the inhibition mechanism and binding behavior of paeonol to tyrosinase and its anti-browning property by multi-spectroscopic and molecular docking methods
In this study, the inhibition mechanism and binding behavior of paeonol to tyrosinase and its anti-browning property were investigated using multi-spectroscopic and molecular docking methods. Activity assay and kinetic results confirmed paeonol as a reversible mixed-type tyrosinase inhibitor. Results of the mechanistic studies were clarified using fluorescence quenching, synchronous fluorescence, CD spectra and 3D fluorescence, and showed that the binding of paeonol to tyrosinase might change the chromophore microenvironment and conformation of tyrosinase to inhibit enzyme catalytic activity. Molecular docking results reve...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Xiaofeng Min Li Lu Xuetao Xu Yi Wen Xi Zheng Source Type: research

The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum
Fungal Genet Biol. 2023 Sep 16:103837. doi: 10.1016/j.fgb.2023.103837. Online ahead of print.ABSTRACTEpigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacety...
Source: Fungal Genetics and Biology - September 18, 2023 Category: Biology Authors: Dianiris Luciano-Rosario Omer Barda Joanna Tannous Dean Frawley Özgür Bayram Dov Prusky Edward Sionov Nancy P Keller Source Type: research

Investigation on the inhibition mechanism and binding behavior of paeonol to tyrosinase and its anti-browning property by multi-spectroscopic and molecular docking methods
In this study, the inhibition mechanism and binding behavior of paeonol to tyrosinase and its anti-browning property were investigated using multi-spectroscopic and molecular docking methods. Activity assay and kinetic results confirmed paeonol as a reversible mixed-type tyrosinase inhibitor. Results of the mechanistic studies were clarified using fluorescence quenching, synchronous fluorescence, CD spectra and 3D fluorescence, and showed that the binding of paeonol to tyrosinase might change the chromophore microenvironment and conformation of tyrosinase to inhibit enzyme catalytic activity. Molecular docking results reve...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Xiaofeng Min Li Lu Xuetao Xu Yi Wen Xi Zheng Source Type: research

The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum
Fungal Genet Biol. 2023 Sep 16:103837. doi: 10.1016/j.fgb.2023.103837. Online ahead of print.ABSTRACTEpigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacety...
Source: Fungal Genetics and Biology - September 18, 2023 Category: Biology Authors: Dianiris Luciano-Rosario Omer Barda Joanna Tannous Dean Frawley Özgür Bayram Dov Prusky Edward Sionov Nancy P Keller Source Type: research

The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum
Fungal Genet Biol. 2023 Sep 16:103837. doi: 10.1016/j.fgb.2023.103837. Online ahead of print.ABSTRACTEpigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacety...
Source: Fungal Genetics and Biology - September 18, 2023 Category: Biology Authors: Dianiris Luciano-Rosario Omer Barda Joanna Tannous Dean Frawley Özgür Bayram Dov Prusky Edward Sionov Nancy P Keller Source Type: research

Cold atmospheric plasma fumigation suppresses postharvest apple Botrytis cinerea by triggering intracellular reactive oxygen species and mitochondrial calcium
In this study, the effects of cold atmospheric plasma (CAP) fumigation on apple B. cinerea under different exposure times and intensities were investigated. The growth of B. cinerea in vitro and in vivo was significantly suppressed by the CAP fumigation at least 700 μL/L for 5 min. To reveal the possible mechanism of antifungal activity of CAP fumigation, the pathogen was exposed to 700 μL/L and 1000 μL/L for 5 min, respectively. The results indicated that the CAP-treated spores of the pathogen underwent shrinkage, cell membrane collapse and cytoplasmic vacuolation. The results obtained from the fluorescent probe assay ...
Source: International Journal of Food Microbiology - September 16, 2023 Category: Food Science Authors: Jiankang Cao Qiong Fang Chenrui Han Chongshan Zhong Source Type: research

Exploring the impact of carbon emissions and co-macroeconomic determinants on China's sustainable apple export
Environ Sci Pollut Res Int. 2023 Sep 14. doi: 10.1007/s11356-023-29497-3. Online ahead of print.ABSTRACTChina is the foremost global consumer, producer, and exporter of fresh apples. In 2021, China produced roughly 44 million tons of apples and exported just over 1 million tons, a nearly 2% increase over the previous year. However, the ongoing COVID-19 pandemic has had a detrimental impact on global trade and has led to a decrease in China's agricultural exports. The present study aims to contribute to the existing body of literature by analyzing plausible macroeconomic determinants that might impact China's apple exports....
Source: Environmental Science and Pollution Research International - September 14, 2023 Category: Environmental Health Authors: Zaid Ashiq Khan Mansoor Ahmed Koondhar Aftab Khan Zixin Zhang Uzair Ali Zhanar Nurgazina Tianjun Liu Source Type: research

Exploring the impact of carbon emissions and co-macroeconomic determinants on China's sustainable apple export
Environ Sci Pollut Res Int. 2023 Sep 14. doi: 10.1007/s11356-023-29497-3. Online ahead of print.ABSTRACTChina is the foremost global consumer, producer, and exporter of fresh apples. In 2021, China produced roughly 44 million tons of apples and exported just over 1 million tons, a nearly 2% increase over the previous year. However, the ongoing COVID-19 pandemic has had a detrimental impact on global trade and has led to a decrease in China's agricultural exports. The present study aims to contribute to the existing body of literature by analyzing plausible macroeconomic determinants that might impact China's apple exports....
Source: Environmental Science and Pollution Research International - September 14, 2023 Category: Environmental Health Authors: Zaid Ashiq Khan Mansoor Ahmed Koondhar Aftab Khan Zixin Zhang Uzair Ali Zhanar Nurgazina Tianjun Liu Source Type: research