Cancers, Vol. 11, Pages 728: TCam-2 Cells Deficient for SOX2 and FOXA2 Are Blocked in Differentiation and Maintain a Seminoma-Like Cell Fate In Vivo

In this study, we generated TCam-2 cells double-deficient for SOX2 and FOXA2 using the CRISPR/Cas9 technique and xenografted those cells into the flank of nude mice. Upon loss of SOX2 and FOXA2, TCam-2 maintained a seminoma cell fate for at least twelve weeks, demonstrating that both factors are key players in the reprogramming to an EC-like cell fate. Therefore, our study adds an important piece to the puzzle of GCT development and plasticity, providing interesting insights in what can be expected in a patient, when GCT cells are confronted with different microenvironments.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research

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AbstractThe activity concentrations of natural radionuclides from the238U and232Th series and singly occurring radionuclide40K have been assessed in most commonly used building materials in Kannur district, Kerala, using well established gamma ray spectrometry employing 5  cm × 5 cm scintillation NaI (Tl) detector. The results of the present work include activities of radionuclides viz.40K,226Ra and232Th and associated radiological parameters. The important and expected significant parameters estimated from the activities were radium equivalent activity (Raeq), hazard indexes and dose paramete...
Source: Journal of Radioanalytical and Nuclear Chemistry - Category: Nuclear Medicine Source Type: research
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Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Publication date: Available online 17 September 2019Source: Journal of Materials Research and TechnologyAuthor(s): Lu Liu, Jiajun Qiu, Xuanyong LiuAbstractGraphene oxide (GO) has been widely investigated in biomedical fields due to its excellent biocompatibility. In the present study, GO films were fabricated on titanium surface by cathode electrophoretic deposition with the help of Mg2+ and Al3+. Responses of rat bone marrow mesenchymal stem cells to GO films with different alkali treatment, including urea hydrothermal treatment and sodium hydroxide hydrothermal treatment, were investigated. The results indicated that GO ...
Source: Journal of Materials Research and Technology - Category: Materials Science Source Type: research
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Source: Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis - Category: Cytology Source Type: research
Publication date: 17 September 2019Source: Cell Reports, Volume 28, Issue 12Author(s): Banushree Kumar, Simon J. ElsässerSummaryTo understand the epigenomic foundation of naive pluripotency, we implement a quantitative multiplexed chromatin immunoprecipitation sequencing (ChIP-seq) method comparing mouse embryonic stem cells (ESCs) grown in 2i versus 2i/serum and serum conditions. MINUTE-ChIP has a large linear dynamic range for accurately quantifying relative differences in genome-wide histone modification patterns across multiple pooled samples. We find compelling evidence for a broad H3 lysine 27 trimethylation (H3...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 17 September 2019Source: Cell Reports, Volume 28, Issue 12Author(s): Carlos Zeledon, Xiaojuan Sun, Cédric Plutoni, Gregory EmerySummaryCollective cell migration is involved in various developmental and pathological processes, including the dissemination of various cancer cells. During Drosophila melanogaster oogenesis, a group of cells called border cells migrate collectively toward the oocyte. Herein, we show that members of the Arf family of small GTPases and some of their regulators are required for normal border cell migration. Notably, we found that the ArfGAP Drongo and its GTPase-activating ...
Source: Cell Reports - Category: Cytology Source Type: research
We report a high-content screen for small-molecule regulators of the axonal transport of mitochondria. Six compounds enhanced mitochondrial transport in the sub-micromolar range, acting via three cellular targets: F-actin, Tripeptidyl peptidase 1 (TPP1), or Aurora Kinase B (AurKB). Pharmacological inhibition or small hairpin RNA (shRNA) knockdown of each target promotes mitochondrial axonal transport in rat hippocampal neurons and induced pluripotent stem cell (iPSC)-derived human cortical neurons and enhances mitochondrial transport in iPSC-derived motor neurons from an amyotrophic lateral sclerosis (ALS) patient bearing ...
Source: Cell Reports - Category: Cytology Source Type: research
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Source: Cell Reports - Category: Cytology Source Type: research
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Source: Cell Reports - Category: Cytology Source Type: research
ConclusionsWe conclude that AATG is a promising and safe modality for the treatment of the painful scar. There is an abundance of low-level evidence to support its use as an alternative treatment but there is a lack of high-level evidence at present to support its standard use. Future long-term randomized controlled trials with analgesic scores as the primary outcome measures are required to assess long-term efficacy.
Source: Cytotherapy - Category: Cytology Source Type: research
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