Generation of a TBX20 homozygous knockout stem cell line (WAe009-A-1E) by episomal vector-based CRISPR/Cas9 system

Stem Cell Res. 2024 Mar 11;77:103384. doi: 10.1016/j.scr.2024.103384. Online ahead of print.ABSTRACTThe T-box family transcription factor gene TBX20 plays a crucial role in cardiac development and function. TBX20 mutations are associated with congenital heart disease, dilated cardiomyopathy, arrhythmias, and heart failure. To further study the role of TBX20 in human heart, here we generated a homozygous TBX20 knockout (TBX20-KO) human embryonic stem cell line using the CRISPR/Cas9 system. This TBX20-KO cell line maintains normal morphology, pluripotency, and karyotype, making it a valuable tool for investigating TBX20's role in cardiac biology.PMID:38479330 | DOI:10.1016/j.scr.2024.103384

Source: Cell Research - March 13, 2024 Category: Cytology Authors: Xiaojie Hou Junsheng Mu Source Type: research