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Vegan grade medium component screening and concentration optimization for the fermentation of the probiotic strain Lactobacillus paracasei IMC 502 ® using Design of Experiments
J Ind Microbiol Biotechnol. 2024 Apr 24:kuae016. doi: 10.1093/jimb/kuae016. Online ahead of print.ABSTRACTLactobacillus paracasei IMC502® is a commercially successful probiotic strain, however, there are no reports that investigate growth medium composition in relation to improved biomass production for this strain. The major outcome of the present study is the design and optimization of a growth medium based on vegan components to be used in the cultivation of Lactobacillus paracasei IMC502®, by using Design of Experiments (DoE). Besides comparing different carbon sources, the use of plant-based peptones as nitrogen sou...
Source: Journal of Industrial Microbiology and Biotechnology - April 24, 2024 Category: Microbiology Authors: Darshankumar Parecha Alberto Alfano Donatella Cimini Chiara Schiraldi Source Type: research

Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling
J Integr Plant Biol. 2024 Apr 24. doi: 10.1111/jipb.13658. Online ahead of print.ABSTRACTLeaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical ...
Source: Journal of Integrative Plant Biology - April 24, 2024 Category: Biology Authors: Longxi Zhou Guowei Chang Chuncai Shen Wan Teng Xue He Xueqiang Zhao Yanfu Jing Zhixiong Huang Yiping Tong Source Type: research

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination
Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain e... (Source: BMC Plant Biology)
Source: BMC Plant Biology - April 24, 2024 Category: Biology Authors: Wei Gao, Yating Jiang, Xiaohu Yang, Ting Li, Litian Zhang, Shengnan Yan, Jiajia Cao, Jie Lu, Chuanxi Ma, Cheng Chang and Haiping Zhang Tags: Research Source Type: research

Impact of Bacillus cereus SPB-10 on Growth Promotion of Wheat (Triticum aestivum L.) Under Arsenic-Contaminated Soil
This study investigates the impact of bacteria on arsenic reduction in wheat plants, highlighting the potential of microbe-based eco-friendly strategies for plant growth. In the present study, bacterial isolate SPB-10 was survived at high concentration against both form of arsenic (As3+ and As5+). SPB-10 produced 5.2 g/L and 11.3 g/L of exo-polysaccharide at 20 ppm of As3+ and As5+, respectively, whereas qualitative examination revealed the highest siderophores ability. Other PGP attributes such as IAA production were recorded 52.12 mg/L and 95.82 mg/L, phosphate solubilization was 90.23 mg/L and 129 mg/L at 20 ppm of As3+...
Source: Current Microbiology - April 23, 2024 Category: Microbiology Authors: Prabhat K Chauhan Himanshu K Pathak Gopal Dubey Hritik Sharma Sudhir K Upadhyay Source Type: research

Molecules, Vol. 29, Pages 1923: Formulation and Characterization of Soybean Oil-in-Water Emulsions Stabilized Using Gelatinized Starch Dispersions from Plant Sources
In this study, we investigated the effects of gelatinized starch dispersions (GSDs) prepared from six plant sources (indica and japonica rice, wheat, corn, potatoes, and sweet potatoes) on the formulation and stability of oil-in-water (O/W) emulsions. The effect of gelatinization temperature and time conditions of 85&ndash;90 &deg;C for 20 min on the interfacial tension of the two phases was observed. Emulsification was performed using a primary homogenization condition of 10,000 rpm for 5 min, followed by high-pressure homogenization at 100 MPa for five cycles. The effects of higher oil weight fractions (1...
Source: Molecules - April 23, 2024 Category: Chemistry Authors: Ankita Singh Takumi Umeda Isao Kobayashi Tags: Article Source Type: research

Neural Tracing Protein ‐Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons
Chemically conjugating the neural tracing protein WGA-HRP to gold nanoparticle (AuNP) is effective in inducing fast retrograde axonal transport of the nanoparticle in live neurons cultured in microfluidics with microchannels separating the axon side from the cell body side. AbstractNeural tracing proteins like horseradish peroxidase-conjugated wheat germ agglutinin (WGA-HRP) can target the central nervous system (CNS) through anatomic retrograde transport without crossing the blood –brain barrier (BBB). Conjugating WGA-HRP to nanoparticles may enable the creation of BBB-bypassing nanomedicine. Microfluidics and two-photo...
Source: Small - April 22, 2024 Category: Nanotechnology Authors: Wenqian Wang, Md. Musfizur Hassan, Natasha Kapoor ‐Kaushik, Lital Livni, Benjamin Musrie, Jianbo Tang, Zaheri Mahmud, Saluo Lai, Peter Richard Wich, Vaishnavi Ananthanarayanan, Gila Moalem‐Taylor, Guangzhao Mao Tags: Research Article Source Type: research

Separating the Wheat from the Chaff among HLA-DQ Eplets
J Immunol. 2024 Apr 22:ji2400030. doi: 10.4049/jimmunol.2400030. Online ahead of print.ABSTRACTIn transplantation, anti-HLA Abs, especially targeting the DQ locus, are well-known to lead to rejection. These Abs identified by Luminex single Ag assays recognize polymorphic amino acids on HLA, named eplets. The HLA Eplet Registry included 83 DQ eplets, mainly deduced from amino acid sequence alignments, among which 66 have not been experimentally verified. Because eplet mismatch load may improve organ allocation and transplant outcomes, it is imperative to confirm the genuine reactivity of eplets to validate this approach. Ou...
Source: Journal of Immunology - April 22, 2024 Category: Allergy & Immunology Authors: Magali Devriese Fran çois A Lemonnier Julien Lion Caroline Sayegh Emma Fleury Catherine Shofstall Lisa Giraldo Quentin Fiachetti C édric Usureau Hiroko Miyadera Olivier Toutirais Nuala Mooney Dave Lowe Jean-Luc Taupin Source Type: research

Factors driving antimony accumulation in soil-pakchoi and wheat agroecosystems: insights and predictive models
This study investigated the accumulation of Sb in the edible parts of pakchoi and wheat grain by adding exogenous Sb to 21 soils with varying properties. The results revealed a positive correlation between bioavailable Sb (Sbava, extracted by 0.1 M K2HPO4) in soil and Sb in the edible parts of pakchoi (R2 = 0.77, p<0.05) and wheat grain (R2 = 0.54, p<0.05). Both machine learning and traditional multiple regression analysis indicated Sbava was the most critical feature and the main soil properties that contributed to Sb uptake by pakchoi and wheat were CaCO3 and clay, respectively. The advisory food limits for Sb in p...
Source: Environmental Pollution - April 22, 2024 Category: Environmental Health Authors: Tongliang Wu Naichi Zhang Cun Liu Changfeng Ding Peng Zhang Sainan Hu Yihang Huang Zixuan Ge Peixin Cui Yujun Wang Source Type: research

Molecules, Vol. 29, Pages 1895: Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis
In this study, ancestral sequence reconstruction and molecular docking of xylanase and wheat xylan were used to enhance the activity and stability of a thermophilic xylanase. The results indicated that the ancestral enzyme TmxN3 exhibited significantly improved activity and thermal stability. The Vmax increased by 2.7 times, and the catalytic efficiency (Kcat/Km) increased by 1.7 times in comparison to TmxB. After being incubated at 100 &deg;C for 120 min, it still retained 87.3% of its activity, and the half-life in 100 &deg;C was 330 min, while the wild type xylanase was only 55 min. This resulted in an i...
Source: Molecules - April 22, 2024 Category: Chemistry Authors: Guoshuai Hu Xizhi Hong Meixin Zhu Lei Lei Zhenggang Han Yong Meng Jiangke Yang Tags: Article Source Type: research

Effect of different molecular weight β-glucan hydrated with highland barley protein on the quality and in vitro starch digestibility of whole wheat bread
This study investigated the effects of β-glucan and highland barley protein of different molecular weights (2 × 104, 1 × 105, and 3 × 105 Da) and different hydrate methods (pre-hydrate and not pre-hydrate) on the characteristics of whole wheat dough and bread. The mixing properties and rheological properties demonstrated that β-glucan pre-hydrated with highland barley protein were able to reduce the dough tan δ, reduce the dough viscoelasticity, while enhance the gluten network structure and dough deformation resistance. Compared to the control sample, the medium molecular weight pre-hydrate bread had a better specif...
Source: International Journal of Biological Macromolecules - April 21, 2024 Category: Biochemistry Authors: Mengfei Gao Zhongbo Hu Yueyue Yang Zhengyu Jin Aiquan Jiao Source Type: research