Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling
J Integr Plant Biol. 2024 Apr 24. doi: 10.1111/jipb.13658. Online ahead of print.ABSTRACTLeaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical ...
Source: Journal of Integrative Plant Biology - April 24, 2024 Category: Biology Authors: Longxi Zhou Guowei Chang Chuncai Shen Wan Teng Xue He Xueqiang Zhao Yanfu Jing Zhixiong Huang Yiping Tong Source Type: research
Identification of the succinate-CoA ligase protein gene family reveals that TaSUCL1-1 positively regulate cadmium resistance in wheat
This study provided a comprehensive analysis of the TaSUCL1 gene family. Within the TaSUCL1 genes, the exon-intron structure and motif composition exhibited significant similarity among members of the same evolutionary branch. Homology analysis and phylogenetic comparison of the SUCL1 genes in different plants offered valuable insights for studying the evolutionary characteristics of the SUCL1 genes. The expression levels of the TaSUCL1 genes in different tissues and under various metal stress conditions reveal its important role in plant growth and development. Gene function analysis demonstrated that TaSUCL1-1 silenced w...
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Liuliu Wu Lifan Cao Ye Tao Halyna Zhatova Haiyan Hu Chengwei Li Source Type: research
Purple rice starch in wheat: Effect on retrogradation dependent on addition amount
In conclusion, the diverse effects of varying PRS proportions on WS alter the texture and characteristics of starch-based foods, underscoring the potential of starch blending for improved applications.PMID:38657931 | DOI:10.1016/j.ijbiomac.2024.131788 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Shuqing Cai Qiqi Su Qian Zhou Qingfei Duan Weijuan Huang Wei Huang Xiuping Xie Pei Chen Fengwei Xie Source Type: research
Fabrication and characterization of novel prolamin nanoparticle-filled starch gels incorporating resveratrol
This study aimed to improve the mechanical properties of wheat starch gels (WSG) and the stability and bioaccessibility of resveratrol (Res) in prolamin nanoparticles. Res-loaded gliadin (Gli), zein, deamidated gliadin (DG) and deamidated zein (DZ) nanoparticles were filled in WSG. The hardness, G' and G'' of WSG were notably increased. It can be attributed to the more ordered and stable structure induced by the interaction of prolamin nanoparticles and starch. The Res retention of nanoparticles and nanoparticle-filled starch gels was at least 24.6 % and 36.0 % higher than free Res upon heating. When exposed to ultraviolet...
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Lin Zhang Luzhen Chen Shiyi Li Zhenyu Yu Yibin Zhou Yongquan Wang Source Type: research
A phase-separated protein hub modulates resistance to Fusarium head blight in wheat
Cell Host Microbe. 2024 Apr 16:S1931-3128(24)00119-7. doi: 10.1016/j.chom.2024.04.002. Online ahead of print.ABSTRACTFusarium head blight (FHB) is a devastating wheat disease. Fhb1, the most widely applied genetic locus for FHB resistance, is conferred by TaHRC of an unknown mode of action. Here, we show that TaHRC alleles distinctly drive liquid-liquid phase separation (LLPS) within a proteinaceous complex, determining FHB susceptibility or resistance. TaHRC-S (susceptible) exhibits stronger LLPS ability than TaHRC-R (resistant), and this distinction is further intensified by fungal mycotoxin deoxynivalenol, leading to op...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Yi He Xiujuan Yang Xiaobo Xia Yuhua Wang Yifan Dong Lei Wu Peng Jiang Xu Zhang Cong Jiang Hongxiang Ma Wujun Ma Cong Liu Ryan Whitford Matthew R Tucker Zhengguang Zhang Gang Li Source Type: research
Vegan grade medium component screening and concentration optimization for the fermentation of the probiotic strain Lactobacillus paracasei IMC 502 ® using Design of Experiments
J Ind Microbiol Biotechnol. 2024 Apr 24:kuae016. doi: 10.1093/jimb/kuae016. Online ahead of print.ABSTRACTLactobacillus paracasei IMC502® is a commercially successful probiotic strain, however, there are no reports that investigate growth medium composition in relation to improved biomass production for this strain. The major outcome of the present study is the design and optimization of a growth medium based on vegan components to be used in the cultivation of Lactobacillus paracasei IMC502®, by using Design of Experiments (DoE). Besides comparing different carbon sources, the use of plant-based peptones as nitrogen sou...
Source: Journal of Industrial Microbiology and Biotechnology - April 24, 2024 Category: Microbiology Authors: Darshankumar Parecha Alberto Alfano Donatella Cimini Chiara Schiraldi Source Type: research
Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling
J Integr Plant Biol. 2024 Apr 24. doi: 10.1111/jipb.13658. Online ahead of print.ABSTRACTLeaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical ...
Source: Journal of Integrative Plant Biology - April 24, 2024 Category: Biology Authors: Longxi Zhou Guowei Chang Chuncai Shen Wan Teng Xue He Xueqiang Zhao Yanfu Jing Zhixiong Huang Yiping Tong Source Type: research
A phase-separated protein hub modulates resistance to Fusarium head blight in wheat
Cell Host Microbe. 2024 Apr 16:S1931-3128(24)00119-7. doi: 10.1016/j.chom.2024.04.002. Online ahead of print.ABSTRACTFusarium head blight (FHB) is a devastating wheat disease. Fhb1, the most widely applied genetic locus for FHB resistance, is conferred by TaHRC of an unknown mode of action. Here, we show that TaHRC alleles distinctly drive liquid-liquid phase separation (LLPS) within a proteinaceous complex, determining FHB susceptibility or resistance. TaHRC-S (susceptible) exhibits stronger LLPS ability than TaHRC-R (resistant), and this distinction is further intensified by fungal mycotoxin deoxynivalenol, leading to op...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Yi He Xiujuan Yang Xiaobo Xia Yuhua Wang Yifan Dong Lei Wu Peng Jiang Xu Zhang Cong Jiang Hongxiang Ma Wujun Ma Cong Liu Ryan Whitford Matthew R Tucker Zhengguang Zhang Gang Li Source Type: research
Vegan grade medium component screening and concentration optimization for the fermentation of the probiotic strain Lactobacillus paracasei IMC 502 ® using Design of Experiments
J Ind Microbiol Biotechnol. 2024 Apr 24:kuae016. doi: 10.1093/jimb/kuae016. Online ahead of print.ABSTRACTLactobacillus paracasei IMC502® is a commercially successful probiotic strain, however, there are no reports that investigate growth medium composition in relation to improved biomass production for this strain. The major outcome of the present study is the design and optimization of a growth medium based on vegan components to be used in the cultivation of Lactobacillus paracasei IMC502®, by using Design of Experiments (DoE). Besides comparing different carbon sources, the use of plant-based peptones as nitrogen sou...
Source: Journal of Industrial Microbiology and Biotechnology - April 24, 2024 Category: Microbiology Authors: Darshankumar Parecha Alberto Alfano Donatella Cimini Chiara Schiraldi Source Type: research
Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcusabyssi
Carbohydr Res. 2024 Apr 22;539:109122. doi: 10.1016/j.carres.2024.109122. Online ahead of print.ABSTRACTThe genomic screening of hyper-thermophilic Pyrococcus abyssi showed uncharacterized novel α-amylase sequences. Homology modelling analysis revealed that the α-amylase from P. abyssi consists of an N-terminal GH57 catalytic domain, α-amylase central, and C-terminal domain. Current studies emphasize in-silico structural and functional analysis, recombinant expression, characterization, structural studies through CD spectroscopy, and ligand binding studies of the novel α-amylase from P. abyssi. The soluble expression o...
Source: Carbohydrate Research - April 24, 2024 Category: Genetics & Stem Cells Authors: Mohsin Shad Hafiz Muzzammel Rehman Muhammad Waheed Akhtar Muhammad Sajjad Source Type: research
Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling
J Integr Plant Biol. 2024 Apr 24. doi: 10.1111/jipb.13658. Online ahead of print.ABSTRACTLeaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical ...
Source: Journal of Integrative Plant Biology - April 24, 2024 Category: Biology Authors: Longxi Zhou Guowei Chang Chuncai Shen Wan Teng Xue He Xueqiang Zhao Yanfu Jing Zhixiong Huang Yiping Tong Source Type: research
Vegan grade medium component screening and concentration optimization for the fermentation of the probiotic strain Lactobacillus paracasei IMC 502 ® using Design of Experiments
J Ind Microbiol Biotechnol. 2024 Apr 24:kuae016. doi: 10.1093/jimb/kuae016. Online ahead of print.ABSTRACTLactobacillus paracasei IMC502® is a commercially successful probiotic strain, however, there are no reports that investigate growth medium composition in relation to improved biomass production for this strain. The major outcome of the present study is the design and optimization of a growth medium based on vegan components to be used in the cultivation of Lactobacillus paracasei IMC502®, by using Design of Experiments (DoE). Besides comparing different carbon sources, the use of plant-based peptones as nitrogen sou...
Source: Journal of Industrial Microbiology and Biotechnology - April 24, 2024 Category: Microbiology Authors: Darshankumar Parecha Alberto Alfano Donatella Cimini Chiara Schiraldi Source Type: research
Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling
J Integr Plant Biol. 2024 Apr 24. doi: 10.1111/jipb.13658. Online ahead of print.ABSTRACTLeaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical ...
Source: Journal of Integrative Plant Biology - April 24, 2024 Category: Biology Authors: Longxi Zhou Guowei Chang Chuncai Shen Wan Teng Xue He Xueqiang Zhao Yanfu Jing Zhixiong Huang Yiping Tong Source Type: research
