Molecules, Vol. 29, Pages 1895: Improving the Quality of Wheat Flour Bread by a Thermophilic Xylanase with Ultra Activity and Stability Reconstructed by Ancestral Sequence and Computational-Aided Analysis
In this study, ancestral sequence reconstruction and molecular docking of xylanase and wheat xylan were used to enhance the activity and stability of a thermophilic xylanase. The results indicated that the ancestral enzyme TmxN3 exhibited significantly improved activity and thermal stability. The Vmax increased by 2.7 times, and the catalytic efficiency (Kcat/Km) increased by 1.7 times in comparison to TmxB. After being incubated at 100 °C for 120 min, it still retained 87.3% of its activity, and the half-life in 100 °C was 330 min, while the wild type xylanase was only 55 min. This resulted in an i...
Source: Molecules - April 22, 2024 Category: Chemistry Authors: Guoshuai Hu Xizhi Hong Meixin Zhu Lei Lei Zhenggang Han Yong Meng Jiangke Yang Tags: Article Source Type: research
Molecules, Vol. 29, Pages 1880: Structural and Rheological Characterization of Vegetable Crispbread Enriched with Legume Pur & eacute;e
Molecules, Vol. 29, Pages 1880: Structural and Rheological Characterization of Vegetable Crispbread Enriched with Legume Purée
Molecules doi: 10.3390/molecules29081880
Authors:
Karolina Szulc
Sabina Galus
Crispbread is gaining popularity as a healthy snack or bread substitute. This is a lightweight dry type of flat food that stays fresh for a very long time due to its lack of water and usually contains different types of grain flour, including gluten-containing wheat or rye flour. The incorporation of legume purée into crispbread represents an innovative approach to enhancing the nutriti...
Source: Molecules - April 20, 2024 Category: Chemistry Authors: Karolina Szulc Sabina Galus Tags: Article Source Type: research
Correction: Rapid and cost-effective molecular karyotyping in wheat, barley, and their crossprogeny by chromosome-specific multiplex PCR
(Source: Plant Methods)
Source: Plant Methods - April 20, 2024 Category: Biology Authors: Mohammad Ali, D ávid Polgári, Adél Sepsi, Levente Kontra, Ágnes Dalmadi, Zoltán Havelda, László Sági and András Kis Tags: Correction Source Type: research
Presence of the neurotoxin β-N-methylamino-L-alanine in irrigation water and accumulation in cereal grains with human exposure risk
Environ Sci Pollut Res Int. 2024 Apr 18. doi: 10.1007/s11356-024-33188-y. Online ahead of print.ABSTRACTThe present study demonstrates the presence of the neurotoxin β-N-methylamino-L-alanine and its cyanobacterial producers in irrigation water and grains of some cereal plants from farmlands irrigated with Nile River water in Egypt. BMAA detected by LC-MS/MS in phytoplankton samples was found at higher concentrations of free form (0.84-11.4 μg L-1) than of protein-bound form (0.16-1.6 μg L-1), in association with the dominance of cyanobacteria in irrigation water canals. Dominant cyanobacterial species isolated from the...
Source: Environmental Science and Pollution Research International - April 18, 2024 Category: Environmental Health Authors: Zakaria A Mohamed Rehab O Elnour Saad Alamri Mohamed Hashem Alexandre Campos Vitor Vasconcelos Hanan Badawye Source Type: research
High Fat Diet –Wheat Gliadin Interaction and its Implication for Obesity and Celiac Disease Onset: In Vivo Studies
Wheat gluten may play a role for development of life-style related diseases in populations on a high fat diet. Mice feed high fat diet show that wheat gliadin consumption affects glucose and lipid metabolic homeostasis, alters the gut microbiota and the immune cell profile in liver. AbstractThe intestinal immune system plays a crucial role in obesity and insulin resistance. An altered intestinal immunity is associated with changes to the gut microbiota, barrier function, and tolerance to luminal antigens. Lipid metabolism and its unbalance can also contribute to acute and chronic inflammation in different conditions. In ce...
Source: Molecular Nutrition and Food Research - April 18, 2024 Category: Food Science Authors: Yuri Haneishi,
Lucia Treppiccione,
Francesco Maurano,
Diomira Luongo,
Junki Miyamoto,
Mauro Rossi Tags: Review Source Type: research
QTL mapping for the flag leaf-related traits using RILs derived from Trititrigia germplasm line SN304 and wheat cultivar Yannong15 in multiple environments
Developing and enriching genetic resources plays important role in the crop improvement. The flag leaf affects plant architecture and contributes to the grain yield of wheat (Triticum aestivum L.). The genetic im... (Source: BMC Plant Biology)
Source: BMC Plant Biology - April 18, 2024 Category: Biology Authors: Xia Zhang, Piyi Xing, Caicai Lin, Honggang Wang, Yinguang Bao and Xingfeng Li Tags: Research Source Type: research
Mechanism of benzoxazinoids affecting the growth and development of Fusarium oxysporum f. sp. fabae
Plant Mol Biol. 2024 Apr 17;114(3):42. doi: 10.1007/s11103-024-01439-8.ABSTRACTContinuous cropping of faba bean (Vicia faba L.) has led to a high incidence of wilt disease. The implementation of an intercropping system involving wheat and faba bean can effectively control the propagation of faba bean wilt disease. To investigate the mechanisms of wheat in mitigating faba bean wilt disease in a wheat-faba bean intercropping system. A comprehensive investigation was conducted to assess the temporal variations in Fusarium oxysporum f. sp. fabae (FOF) on the chemotaxis of benzoxazinoids (BXs) and wheat root through indoor cult...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Zixuan Cen Bijie Hu Siyin Yang Guanglei Ma Yiran Zheng Yan Dong Source Type: research
Mechanism of benzoxazinoids affecting the growth and development of Fusarium oxysporum f. sp. fabae
Plant Mol Biol. 2024 Apr 17;114(3):42. doi: 10.1007/s11103-024-01439-8.ABSTRACTContinuous cropping of faba bean (Vicia faba L.) has led to a high incidence of wilt disease. The implementation of an intercropping system involving wheat and faba bean can effectively control the propagation of faba bean wilt disease. To investigate the mechanisms of wheat in mitigating faba bean wilt disease in a wheat-faba bean intercropping system. A comprehensive investigation was conducted to assess the temporal variations in Fusarium oxysporum f. sp. fabae (FOF) on the chemotaxis of benzoxazinoids (BXs) and wheat root through indoor cult...
Source: Mol Biol Cell - April 17, 2024 Category: Molecular Biology Authors: Zixuan Cen Bijie Hu Siyin Yang Guanglei Ma Yiran Zheng Yan Dong Source Type: research
Characterization of a wheat stable QTL for spike length and its genetic effects on yield-related traits
In this study, a major stable quantitative trait locus (QTL) for SL, i.e., qSl-2B, was detected in... (Source: BMC Plant Biology)
Source: BMC Plant Biology - April 17, 2024 Category: Biology Authors: Hongke Ding, Chenyang Wang, Yibiao Cai, Kai Yu, Haibo Zhao, Faxiang Wang, Xinyao Shi, Jiajia Cheng, Han Sun, Yongzhen Wu, Ran Qin, Cheng Liu, Chunhua Zhao, Xiaohui Sun and Fa Cui Tags: Research Source Type: research
Effects of Streptomyces sp. HU2014 inoculation on wheat growth and rhizosphere microbial diversity under hexavalent chromium stress
Ecotoxicol Environ Saf. 2024 Apr 15;276:116313. doi: 10.1016/j.ecoenv.2024.116313. Online ahead of print.ABSTRACTWheat (Triticum aestivum L.) is a major foodstuff for over 40% of the world's population. However, hexavalent chromium [Cr(VI)] in contaminated soil significantly affects wheat production and its ecological environment. Streptomyces sp. HU2014 was first used to investigate the effects of Cr (VI) stress on wheat growth. We analyzed the Cr(VI) concentration, physicochemical properties of wheat and soil, total Cr content, and microbial community structures during their interactions. HU2014 reduced the toxicity of C...
Source: Ecotoxicology and Environmental Safety - April 16, 2024 Category: Environmental Health Authors: Hongxia Zhu Linfeng Hu Yunlong Wang Peipei Mei Feng Zhou Tetiana Rozhkova Chengwei Li Source Type: research
A < em > Burkholderia cenocepacia < /em > -like environmental isolate strongly inhibits the plant fungal pathogen < em > Zymoseptoria tritici < /em >
Appl Environ Microbiol. 2024 Apr 16:e0222223. doi: 10.1128/aem.02222-23. Online ahead of print.ABSTRACTFungal phytopathogens cause significant reductions in agricultural yields annually, and overusing chemical fungicides for their control leads to environmental pollution and the emergence of resistant pathogens. Exploring natural isolates with strong antagonistic effects against pathogens can improve our understanding of their ecology and develop new treatments for the future. We isolated and characterized a novel bacterial strain associated with the species Burkholderia cenocepacia, termed APO9, which strongly inhibits Zy...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Tingting Song Suyash Gupta Yael Sorokin Omer Frenkel Eddie Cytryn Jonathan Friedman Source Type: research
Characterization of < em > Amycolatopsis < /em > 75iv2 dye-decolorizing peroxidase on < em > O < /em > -glycosides
Appl Environ Microbiol. 2024 Apr 16:e0020524. doi: 10.1128/aem.00205-24. Online ahead of print.ABSTRACTDye-decolorizing peroxidases are heme peroxidases with a broad range of substrate specificity. Their physiological function is still largely unknown, but a role in the depolymerization of plant cell wall polymers has been widely proposed. Here, a new expression system for bacterial dye-decolorizing peroxidases as well as the activity with previously unexplored plant molecules are reported. The dye-decolorizing peroxidase from Amycolatopsis 75iv2 (DyP2) was heterologously produced in the Gram-positive bacterium Streptomyce...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Silja V älimets Peicheng Sun Ludovika Jessica Virginia Gijs van Erven Mark G Sanders Mirjam A Kabel Clemens Peterbauer Source Type: research
Effects of Streptomyces sp. HU2014 inoculation on wheat growth and rhizosphere microbial diversity under hexavalent chromium stress
Ecotoxicol Environ Saf. 2024 Apr 15;276:116313. doi: 10.1016/j.ecoenv.2024.116313. Online ahead of print.ABSTRACTWheat (Triticum aestivum L.) is a major foodstuff for over 40% of the world's population. However, hexavalent chromium [Cr(VI)] in contaminated soil significantly affects wheat production and its ecological environment. Streptomyces sp. HU2014 was first used to investigate the effects of Cr (VI) stress on wheat growth. We analyzed the Cr(VI) concentration, physicochemical properties of wheat and soil, total Cr content, and microbial community structures during their interactions. HU2014 reduced the toxicity of C...
Source: Ecotoxicology and Environmental Safety - April 16, 2024 Category: Environmental Health Authors: Hongxia Zhu Linfeng Hu Yunlong Wang Peipei Mei Feng Zhou Tetiana Rozhkova Chengwei Li Source Type: research
Ozone variability, its formation potential and crops losses in the himalayan foothills
AbstractSurface ozone observations in Doon Valley (Dehradun: 30.3oN, 78.0oE, 700 m), which acts as a bridge between the Himalayas and the Indo-Gangetic Plain, showed daytime higher values, suggesting a typical urban behaviour in proximity of the Himalayas. Ozone exhibited a maximum in spring (49.2 ± 24.8 ppbv in May) with an hourly average of more than 110 ppbv, followed by a secondary maximum in autumn and the lowest level occurring in the summer-monsoon (~ 13 ppbv in July-August). Ozone levels exceeded the 8-hour National Air Quality Standard limit (50 ppbv) throughout the year, except in July-September. The obs...
Source: Air Quality, Atmosphere and Health - April 16, 2024 Category: Environmental Health Source Type: research
Environmental effects and their impact on yield in adjacent experimental plots of high-stem and short-stem wheat varieties
In regional wheat trials, when short-stem wheat varieties and high-stem wheat varieties are planted adjacent to each other in small plots, changes in their marginal plot environment can lead to bias in yield e... (Source: BMC Plant Biology)
Source: BMC Plant Biology - April 16, 2024 Category: Biology Authors: Xiujuan Ren, Xinhua Li, Xingqi Ou and Zijuan Wang Tags: Research Source Type: research
