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Total 76 results found since Jan 2013.

SiRNA Transfection with Cholesterol Modification from Electrospun PET Facilitates Gene Silencing in Human Aortic Smooth Muscle Cells
Anastomotic intimal hyperplasia (AIH) remains the leading cause for prosthetic arterial graft failure. RNAi is a promising tool to silence genes such as Thrombospondin-2 (TSP-2), previously shown by our group to be upregulated in AIH. The present study is aimed at delivering modified TSP-2 siRNA (3’cholesterol tagged TSP-2 siRNA) from electrospun polyethylene terephthalate (ePET) and therefore knocking down TSP-2 gene and ultimately inhibiting AIH.
Source: Journal of Surgical Research - January 10, 2013 Category: Surgery Authors: M.C. Chun, H.S. Oliver-Allen, C.S. Nabzdyk, S.G. Pathan, M.D. Phaneuf, L. Pradhan-Nabzdyk, F.W. LoGerfo Source Type: research

SiRNA Transfection With Cholesterol Modification From Electrospun PET Facilitates Gene Silencing in Human Aortic Smooth Muscle Cells
Anastomotic intimal hyperplasia (AIH) remains the leading cause for prosthetic arterial graft failure. RNAi is a promising tool to silence genes such as Thrombospondin-2 (TSP-2), previously shown by our group to be upregulated in AIH. The present study is aimed at delivering modified TSP-2 siRNA (3’cholesterol tagged TSP-2 siRNA) from electrospun polyethylene terephthalate (ePET) and therefore knocking down TSP-2 gene and ultimately inhibiting AIH.
Source: Journal of Surgical Research - January 10, 2013 Category: Surgery Authors: M.C. Chun, H.S. Oliver-Allen, C.S. Nabzdyk, S.G. Pathan, M.D. Phaneuf, L. Pradhan-Nabzdyk, F.W. LoGerfo Tags: Oral Session: Thursday 2/7 7:30 AM to 9:30 AM Source Type: research

Comparative Analysis of Polymers for siRNA Delivery in Vascular Smooth Muscle Cells
The use of short interfering RNA (siRNA) to degrade mRNA in the cell cytoplasm and transiently attenuate intracellular proteins shows promise in the inhibition of vascular pathogenesis. However, a critical obstacle for therapeutic application is a safe and effective delivery system. Biodegradable polymers are promising alternative molecular carriers for genetic material. Here we aim to perform a comparative analysis of poly(B-amino ester) (PBAE) and polyethylenimine (PEI) polymers in their efficacy for vascular smooth muscle cell (VSMC) transfection using siRNA against the GAPDH housekeeping gene as our test target.
Source: Journal of Surgical Research - July 17, 2015 Category: Surgery Authors: Lindsay M. Bools, Richard K. Fisher, Oscar H. Grandas, Stacy S. Kirkpatrick, Joshua D. Arnold, Mitchell H. Goldman, Michael B. Freeman, Deidra J.H. Mountain Source Type: research

Suppression of Cell Proliferation and Macrophage Infiltration with Localized siRNA Delivery in a Murine Model of Intimal Hyperplasia
Intimal hyperplasia limits the durability of peripheral vascular interventions and is associated with cell proliferation and macrophage infiltration. We use an in vivo rat carotid angioplasty model to evaluate inhibition of intimal hyperplasia with localized siRNA delivery targeting Thrombospondin-2 (TSP2) and evaluate its impact on cell proliferation (Ki-67) and macrophage infiltration (CD68).
Source: Journal of Vascular Surgery - May 19, 2022 Category: Surgery Authors: Jennifer R. Li, Max Zhu, Patric Liang, Kimberly Piper, Mauricio Contreras, Leena Pradhan-Nabzdyk, Frank LoGerfo Tags: PC: Poster Competition Source Type: research

Naked caspase 3 small interfering RNA is effective in cold preservation but not in autotransplantation of porcine kidneys
Conclusions: Naked caspase 3 siRNA administered into the kidney was effective in cold preservation but not enough to protect posttransplant kidneys, which might be because of systemic complementary responses overcoming local effects.
Source: Journal of Surgical Research - July 27, 2012 Category: Surgery Authors: Cheng Yang, Yichen Jia, Tian Zhao, Yinjia Xue, Zitong Zhao, Junlin Zhang, Jina Wang, Xuanchuan Wang, Yongyin Qiu, Miao Lin, Dong Zhu, Guisheng Qi, Yue Qiu, Qunye Tang, Ruiming Rong, Ming Xu, Sujie Ni, Bin Lai, Michael L. Nicholson, Tongyu Zhu, Bin Yang Tags: Transplantation/Immunology Source Type: research

Silencing the livin gene enhances the cytotoxic effects of anticancer drugs on colon cancer cells.
CONCLUSION: siRNA-mediated down-regulation of livin gene expression could significantly suppress colon cancer growth and enhance the cytotoxic effects of anticancer drugs such as 5-FU and L-OHP. The results of this study suggest that silencing livin gene expression in combination with treatment with anticancer drugs might be a novel cancer therapy for colorectal cancer. PMID: 27904848 [PubMed - in process]
Source: Annals of Surgical Treatment and Research - December 3, 2016 Category: Surgery Tags: Ann Surg Treat Res Source Type: research

Murine portal vein catheterization to analyze liver-directed therapies
Conclusions: Liver-directed therapy can provide the selective delivery of siRNA to CRC metastases. EpCAM expression in CRC, but not normal liver, could further selectively target hepatic metastases of epithelial origin.
Source: Journal of Surgical Research - July 18, 2013 Category: Surgery Authors: Joseph D. Valentino, Piotr G. Rychahou, W. Conan Mustain, Victoria A. Elliott, B. Mark Evers Tags: Oncology/Endocrine Source Type: research

Chitosan nanoparticle carrying small interfering RNA to platelet-derived growth factor B mRNA inhibits proliferation of smooth muscle cells in rabbit injured arteries.
In conclusion, transfection of chitosan nanoparticle carrying siRNA against PDGF-B mRNA could inhibit proliferation of vSMCs in the rabbit iliac artery injury model. PMID: 23518855 [PubMed - as supplied by publisher]
Source: Vascular - March 21, 2013 Category: Surgery Authors: Xia H, Jun J, Wen-Ping L, Yi-Feng P, Xiao-Ling C Tags: Vascular Source Type: research

Inhibitory Effect of TLR4 Gene Silencing on Intimal Hyperplasia of Vein Grafting
Conclusion: These results showed that local silencing of TLR4 in the vein grafts could inhibit intimal hyperplasia by downregulating the expression of inflammatory factors in the vein grafts, suggesting that TLR4 can be used as a new target for therapy of vascular intimal hyperplasia.
Source: Vascular and Endovascular Surgery - October 11, 2016 Category: Surgery Authors: Zhu, Z., Xu, R., Zheng, X., Wang, T., Li, D., Wang, Y., Liu, K. Tags: Original Manuscripts Source Type: research

Inhibiting Interleukin-6/Signal Transducers and Activators of Transduction-3/Hypoxia-Inducible Factor-1 α Signaling Pathway Suppressed the Growth of Infantile Hemangioma
Conclusion Our findings indicate that inhibiting the IL-6/STAT3/HIF-1α signaling pathways could suppress IH growth. [...] Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, GermanyArticle in Thieme eJournals: Table of contents  |  Abstract  |  Full text
Source: European Journal of Pediatric Surgery - July 12, 2022 Category: Surgery Authors: Maimaiti, Aziguli Aierken, Yeerfan Zhou, Ling He, Jun Abudureyimu, Abudusaimi Li, Shui-Xue Tags: Original Article Source Type: research

Glucose-regulated protein 78 silencing down-regulates vascular endothelial growth factor/vascular endothelial growth factor receptor 2 pathway to suppress human colon cancer tumor growth
Conclusions: Silencing GRP78 not only inhibits tumor, but also decreases the expression of VEGF and VEGFR2. Collectively, therapy targeting for GRP78 may inhibit the formation of colon cancer tumors via the HIF-1α/VEGF/VEGFR2 pathway.
Source: Journal of Surgical Research - May 30, 2013 Category: Surgery Authors: Li-Jen Kuo, Chin-Sheng Hung, Wei-Yu Chen, Yu-Jia Chang, Po-Li Wei Tags: Oncology/Endocrine Source Type: research

Blockage of CXCR2 suppresses tumor growth of intrahepatic cholangiocellular carcinoma
Conclusion: Our results demonstrated that blocking CXCR2 clearly suppressed the development of ICC. Blocking CXCR2 may be a promising therapeutic approach for ICC.
Source: Surgery - January 6, 2014 Category: Surgery Authors: Hideaki Sueoka, Tadamichi Hirano, Yugo Uda, Yuji Iimuro, Junichi Yamanaka, Jiro Fujimoto Tags: Original Communications Source Type: research

Downregulation of DLGAP1-Antisense RNA 1 Alleviates Vascular Endothelial Cell Injury Via Activation of the Phosphoinositide 3-kinase/Akt Pathway Results from an Acute Limb Ischemia Rat Model.
CONCLUSION: DLGAP1-AS1 silencing could further suppress the oxidative stress, exert an anti-apoptosis effect, and reduce inflammatory reaction, whereby VEC injury is alleviated by activation of the PI3K/Akt pathway in rats with acute lower limb I/R. PMID: 31744785 [PubMed - as supplied by publisher]
Source: PubMed: Eur J Vasc Endovasc ... - November 15, 2019 Category: Surgery Authors: Shen GH, Song Y, Yao Y, Sun QF, Jing B, Wu J, Li SY, Liu SQ, Li HC, Yuan C, Liu GY, Li JB, Liu XY, Wang HY Tags: Eur J Vasc Endovasc Surg Source Type: research

PPAR γ Mediates Protective Effect against Hepatic Ischemia/Reperfusion Injury via NF-κB Pathway
CONCLUSIONS: PPARγ present protective effects on HIRI by attenuating liver injury, inflammatory response, oxidative stress and apoptosis in vivo and in vitro, and its mechanism may be related to down-regulation of NF-κB signaling.PMID:35732295 | DOI:10.1080/08941939.2022.2090033
Source: Journal of Investigative Surgery - June 22, 2022 Category: Surgery Authors: Xinyu Liu Ping Zhang Xianqing Song Hengguan Cui Weixing Shen Source Type: research