Filtered By:
Specialty: Infectious Diseases

This page shows you your search results in order of date. This is page number 9.

Order by Relevance | Date

Total 139 results found since Jan 2013.

ALIX Rescues Budding of a Double PTAP/PPEY L-Domain Deletion Mutant of Ebola VP40: A Role for ALIX in Ebola Virus Egress
Ebola (EBOV) is an enveloped, negative-sense RNA virus belonging to the family Filoviridae that causes hemorrhagic fever syndromes with high-mortality rates. To date, there are no licensed vaccines or therapeutics to control EBOV infection and prevent transmission. Consequently, the need to better understand the mechanisms that regulate virus transmission is critical to developing countermeasures. The EBOV VP40 matrix protein plays a central role in late stages of virion assembly and egress, and independent expression of VP40 leads to the production of virus-like particles (VLPs) by a mechanism that accurately mimics buddi...
Source: The Journal of Infectious Diseases - September 9, 2015 Category: Infectious Diseases Authors: Han, Z., Madara, J. J., Liu, Y., Liu, W., Ruthel, G., Freedman, B. D., Harty, R. N. Tags: MOLECULAR BIOLOGY Source Type: research

Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Listeria monocytogenes is a foodborne pathogen capable of invading a broad range of cell types and replicating within the host cell cytoplasm. This paper describes the colocalization of host cell lamellipodin (Lpd) with intracellular L. monocytogenes detectable 6 h postinfection of epithelial cells. The association was mediated via interactions between both the peckstrin homology (PH) domain in Lpd and phosphatidylinositol (3,4)-bisphosphate [PI(3,4)P2] on the bacterial surface and by interactions between the C-terminal EVH1 (Ena/VASP [vasodilator-stimulated phosphoprotein] homology domain 1) binding domains of Lpd and the...
Source: Infection and Immunity - August 12, 2015 Category: Infectious Diseases Authors: Wang, J., King, J. E., Goldrick, M., Lowe, M., Gertler, F. B., Roberts, I. S. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research

Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus
Publication date: Available online 5 August 2015 Source:The Brazilian Journal of Infectious Diseases Author(s): Camila Michele Appolinario, Suasan Dora Allendorf, Marina Gea Peres, Clovis Reynaldo Fonseca, Acacia Ferreira Vicente, João Marcelo Azevedo de Paula Antunes, José Carlos Figueiredo Pantoja, Jane Megid We have evaluated the efficacy of short-interfering RNAs targeting the nucleoprotein gene and also the brain immune response in treated and non-treated infected mice. Mice were inoculated with wild-type virus, classified as dog (hv2) or vampire bat (hv3) variants and both groups were treated or leaved as...
Source: The Brazilian Journal of Infectious Diseases - August 7, 2015 Category: Infectious Diseases Source Type: research

Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus.
Abstract We have evaluated the efficacy of short-interfering RNAs targeting the nucleoprotein gene and also the brain immune response in treated and non-treated infected mice. Mice were inoculated with wild-type virus, classified as dog (hv2) or vampire bat (hv3) variants and both groups were treated or leaved as controls. No difference was observed in the lethality rate between treated and non-treated groups, although clinical evaluation of hv2 infected mice showed differences in the severity of clinical disease (p=0.0006). Evaluation of brain immune response 5 days post-inoculation in treated hv2 group showed no...
Source: Braz J Infect Dis - August 5, 2015 Category: Infectious Diseases Authors: Appolinario CM, Allendorf SD, Peres MG, Fonseca CR, Vicente AF, de Paula Antunes JM, Pantoja JC, Megid J Tags: Braz J Infect Dis Source Type: research

Induction of interferon lambda in influenza a virus infected cells treated with shRNAs against M1 transcript.
Abstract RNA interference (RNAi) represents a form of post-transcriptional gene silencing mediated by small interfering RNAs (siRNA) and provides a powerful tool to specifically inhibit viral infection. To investigate therapeutic capacity of siRNAs targeting M gene, six vectors with U1-short hairpin RNA (shRNA) expression system were prepared and tested in infected cells and animals. In infected cells, three of six shRNAs targeting M1 gene significantly (P <0,01) reduced the virus titer to 66%, 45% or 21%, respectively. Replication of IAV and levels of M1 RNAs were significantly reduced in the cells transfe...
Source: Acta Virologica - June 28, 2015 Category: Infectious Diseases Authors: Švančarová P, Svetlíková D, Betáková T Tags: Acta Virol Source Type: research

Synergistic Costimulatory Effect of Chlamydia pneumoniae with Carbon Nanoparticles on NLRP3 Inflammasome-Mediated Interleukin-1{beta} Secretion in Macrophages Host Response and Inflammation
This study assessed whether costimulation of C. pneumoniae with CNTs synergistically enhanced IL-1β secretion from macrophages, and determined the molecular mechanism involved. Enhanced IL-1β secretion from C. pneumoniae-infected macrophages by CNTs was dose and time dependent. Transmission electron microscopy revealed that C. pneumoniae and CNTs were engulfed concurrently by macrophages. Inhibitors of actin polymerization or caspase-1, a component of the inflammasome, significantly blocked IL-1β secretion. Gene silencing using small interfering RNA (siRNA) targeting the NLRP3 gene also abolished IL-1β ...
Source: Infection and Immunity - June 15, 2015 Category: Infectious Diseases Authors: Matsuo, J., Nakamura, S., Takeda, S., Ishida, K., Yamazaki, T., Yoshida, M., Chiba, H., Hui, S.-P., Yamaguchi, H. Tags: Host Response and Inflammation Source Type: research

Targeting Human Telomerase Reverse Transcriptase by a Simple siRNA Expression Cassette in HepG2 Cells
Conclusions: Our developed simple SEC was a powerful strategy for screening highly effective RNAi-targeted sequences and showed promise for gene therapy of HCC.
Source: Hepatitis Monthly - March 30, 2015 Category: Infectious Diseases Source Type: research

Receptor tyrosine kinase signaling regulates replication of the peste des petits ruminants virus.
In this study, we found out that blocking the receptor tyrosine kinase (RTK) signaling in Vero cells by tryphostin AG879 impairs the in vitro replication of the peste des petits ruminants virus (PPRV). A reduced virus replication in Trk1-knockdown (siRNA) Vero cells confirmed the essential role of RTK in the virus replication, in particular a specific regulation of viral RNA synthesis. These data represent the first evidence that the RTK signaling regulates replication of a morbillivirus. PMID: 25790054 [PubMed - as supplied by publisher]
Source: Acta Virologica - March 20, 2015 Category: Infectious Diseases Authors: Chaudhary K, Chaubey KK, Singh SV, Kumar N Tags: Acta Virol Source Type: research

Rab14 Regulates Maturation of Macrophage Phagosomes Containing the Fungal Pathogen Candida albicans and Outcome of the Host-Pathogen Interaction Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Avoidance of innate immune defense is an important mechanism contributing to the pathogenicity of microorganisms. The fungal pathogen Candida albicans undergoes morphogenetic switching from the yeast to the filamentous hyphal form following phagocytosis by macrophages, facilitating its escape from the phagosome, which can result in host cell lysis. We show that the intracellular host trafficking GTPase Rab14 plays an important role in protecting macrophages from lysis mediated by C. albicans hyphae. Live-cell imaging of macrophages expressing green fluorescent protein (GFP)-tagged Rab14 or dominant negative Rab14, or with ...
Source: Infection and Immunity - March 17, 2015 Category: Infectious Diseases Authors: Okai, B., Lyall, N., Gow, N. A. R., Bain, J. M., Erwig, L.-P. Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research

CRF-amplified neuronal TLR4/MCP-1 signaling regulates alcohol self-administration.
We report that alcohol-preferring P rats have innately elevated levels of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein-1 (MCP-1) that co-localize in neurons from the CeA and VTA. To examine the potential role of a TLR4/MCP-1 signal, we used Herpes Simplex Virus (HSV) vectors (amplicons) that retain in vivo neurotropism. Infusion of amplicons for TLR4 or MCP-1 siRNA into the CeA or VTA from the P rats inhibited target gene expression and blunted binge drinking. A similarly delivered amplicon for scrambled siRNA did not inhibit TLR4 or MCP-1 expression nor reduce binge drinking, identifying a neuronal TLR4/MC...
Source: Herpes - January 8, 2015 Category: Infectious Diseases Authors: June HL, Liu J, Warnock KT, Bell KA, Balan I, Bollino D, Puche A, Aurelian L Tags: Neuropsychopharmacology Source Type: research

Kinome siRNA screen identifies novel cell-type specific dengue host target genes
Publication date: October 2014 Source:Antiviral Research, Volume 110 Author(s): Yong-Jun Kwon , Jinyeong Heo , Hazel E.E. Wong , Deu John M. Cruz , Sumathy Velumani , Camila T. da Silva , Ana Luiza P. Mosimann , Claudia N. Duarte dos Santos , Lucio H. Freitas-Junior , Katja Fink Dengue is a global emerging infectious disease, with no specific treatment available. To identify novel human host cell targets important for dengue virus infection and replication, an image-based high-throughput siRNA assay screening of a human kinome siRNA library was conducted using human hepatocyte cell line Huh7 infected with a recent dengue...
Source: Antiviral Therapy - November 13, 2014 Category: Infectious Diseases Source Type: research

ATF3 Confers Resistance to Pneumococcal Infection Through Positive Regulation of Cytokine Production
Conclusions. Taken together, we can conclude that ATF3 regulates innate immunity positively upon pneumococcus infection by enhancing TNF-α, IL-1β, and IFN- expression and modulating bacterial clearance.
Source: The Journal of Infectious Diseases - November 7, 2014 Category: Infectious Diseases Authors: Nguyen, C. T., Kim, E.-H., Luong, T. T., Pyo, S., Rhee, D.-K. Tags: BACTERIA Source Type: research

Heat-shock protein 90 promotes nuclear transport of herpes simplex virus 1 capsid protein by interacting with acetylated tubulin.
In this study, we found that Hsp90 inhibitors have potent antiviral activity against standard or drug-resistant HSV-1 strains and viral gene and protein synthesis are inhibited in an early phase. More detailed studies demonstrated that Hsp90 is upregulated by virus entry and it interacts with virus. Hsp90 knockdown by siRNA or treatment with Hsp90 inhibitors significantly inhibited the nuclear transport of viral capsid protein (ICP5) at the early stage of HSV-1 infection. In contrast, overexpression of Hsp90 restored the nuclear transport that was prevented by the Hsp90 inhibitors, suggesting that Hsp90 is required for nuc...
Source: Herpes - June 8, 2014 Category: Infectious Diseases Authors: Zhong M, Zheng K, Chen M, Xiang Y, Jin F, Ma K, Qiu X, Wang Q, Peng T, Kitazato K, Wang Y Tags: PLoS One Source Type: research

RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions.
Abstract The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large subunit of ribonucleotide reductase, an essential enzyme for DNA synthesis. Herpes simplex virus type-1 was isolated from saliva samples and mucocutaneous lesions from infected patients. All mucocutaneous lesions' samples were positive for herpes simple...
Source: Braz J Infect Dis - May 15, 2014 Category: Infectious Diseases Authors: da Silva AP, Lopes JF, de Paula VS Tags: Braz J Infect Dis Source Type: research

Hepatitis B virus x protein induces autophagy via activating death‐associated protein kinase
In conclusion, HBX induces autophagy via activating DAPK in a pathway related to Beclin 1, but not JNK. This new finding should help us to understand the role of autophagy in HBX‐mediated pathogenesis and thus may provide targets for intervening HBX‐related disorders.
Source: Journal of Viral Hepatitis - November 4, 2013 Category: Infectious Diseases Authors: H.‐T Zhang, G. G. Chen, B.‐G. Hu, Z.‐Y. Zhang, J.‐P Yun, M.‐L. He, P. B. S. Lai Tags: Original Article Source Type: research