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Procedure: Kidney Transplant

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Total 64 results found since Jan 2013.

Attenuating Ischemia-Reperfusion Injury in Kidney Transplantation by Perfusing Donor Organs With siRNA Cocktail Solution
Conclusions: In conclusion, this is the first demonstration that perfusing donor organs with an siRNA cocktail solution can induce gene silencing in the kidney and prevent kidneys from extended cold I/R injury in kidney transplantation, highlighting the promise of the clinical application of siRNA-based therapies in the preservation of donor organs.
Source: Transplantation - March 22, 2016 Category: Transplant Surgery Tags: Original Basic Science-General Source Type: research

Innate immunity activation involved in unprotected porcine auto-transplant kidneys preserved by naked caspase-3 siRNA
Conclusions: The activation of innate immunity with amplified inflammatory responses in the caspase-3 siRNA preserved auto-transplant kidneys are associated with increased TLR3, TLR7 and PKR, which might be due to complementary systemic feedback, although persistent actions initiated by short-acting caspase-3 siRNA cannot be completely ruled out. These results provided valuable evidence to guide future siRNA design and pre-clinic studies.
Source: Journal of Translational Medicine - September 13, 2013 Category: Research Authors: Cheng YangLong LiYinjia XueZitong ZhaoTian ZhaoYichen JiaRuiming RongMing XuMichael NicholsonTongyu ZhuBin Yang Source Type: research

siRNA Technology in Kidney Transplantation: Current Status and Future Potential
Abstract Kidney transplantation is one of the most common transplantation operations in the world, accounting for up to 50 % of all transplantation surgeries. To curtail the damage to transplanted organs that is caused by ischemia–reperfusion injury and the recipient’s immune system, small interfering RNA (siRNA) technology is being explored. Importantly, the kidney as a whole is a preferential site for non-specific systemic delivery of siRNA. To date, most attempts at siRNA-based therapy for transplantation-related conditions have remained at the in vitro stage, with only a few of them being advanced into a...
Source: BioDrugs - August 1, 2014 Category: Drugs & Pharmacology Source Type: research

T7 peptide-decorated exosome-based nanocarrier system for delivery of Galectin-9 siRNA to stimulate macrophage repolarization in glioblastoma
ConclusionOverall, T7-Exo/siGalectin-9 promotes macrophage repolarization and restricts the immunosuppression of GBM, thus providing novel insights into and drug delivery system of immunotherapy for GBM.Graphical abstract
Source: Journal of Neuro-Oncology - March 1, 2023 Category: Cancer & Oncology Source Type: research

Novel Complement C5 Small-interfering RNA Lipid Nanoparticle Prolongs Graft Survival in a Hypersensitized Rat Kidney Transplant Model
Conclusions. C5 siRNA-LNP combined with immunosuppressants CsA and DSG downregulated C5 activity and significantly prolonged graft survival compared with control siRNA-LNP with CsA and DSG. Downregulation of C5 expression using C5 siRNA-LNP may be an effective therapeutic approach for AMR.
Source: Transplantation - December 1, 2022 Category: Transplant Surgery Tags: Original Basic Science Source Type: research

Naked caspase 3 small interfering RNA is effective in cold preservation but not in autotransplantation of porcine kidneys
Conclusions: Naked caspase 3 siRNA administered into the kidney was effective in cold preservation but not enough to protect posttransplant kidneys, which might be because of systemic complementary responses overcoming local effects.
Source: Journal of Surgical Research - July 27, 2012 Category: Surgery Authors: Cheng Yang, Yichen Jia, Tian Zhao, Yinjia Xue, Zitong Zhao, Junlin Zhang, Jina Wang, Xuanchuan Wang, Yongyin Qiu, Miao Lin, Dong Zhu, Guisheng Qi, Yue Qiu, Qunye Tang, Ruiming Rong, Ming Xu, Sujie Ni, Bin Lai, Michael L. Nicholson, Tongyu Zhu, Bin Yang Tags: Transplantation/Immunology Source Type: research

Fighting against kidney diseases with small interfering RNA: opportunities and challenges
The significant improvements in siRNA therapy have been achieved, which have great potential applications in humans. The kidney is a comparatively easy target organ of siRNA therapy due to its unique structural and functional characteristics. Here, we reviewed recent achievements in siRNA design, delivery and application with focuses on kidney diseases, in particular kidney transplant-related injuries. In addition, the strategy for increasing serum stability and immune tolerance of siRNA was also discussed. At last, the future challenges of siRNA therapy including organ/tissue/cell-specific delivery and time-controlled sil...
Source: Journal of Translational Medicine - February 1, 2015 Category: Research Authors: Cheng YangChao ZhangZitong ZhaoTongyu ZhuBin Yang Source Type: research

Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention
In conclusion, osmotic burst of inflated complement-damaged cells may occur, but these bursts are most likely a consequence of metabolic collapse of the cell rather than the cause of cell death. The Complement Cell Death Mediator: A Concerted Action of Toxic Moieties Membrane pores caused by complement were first visualized by electron microscopy on red blood cell membranes as large ring structures (22). Similar lesions were viewed on E. coli cell walls (23). Over the years, ample information on the fine ultrastructure of the MAC that can activate cell death has been gathered (24) and has been recently further examined (...
Source: Frontiers in Immunology - April 9, 2019 Category: Allergy & Immunology Source Type: research

Time dependent p53 inhibition determines senescence attenuation and long term outcome after renal ischemia/reperfusion.
In this study we tested the hypothesis that p53 siRNA reduces I/R induced senescence and thereby improves kidney outcome. By comparing the impact of different treatment durations in a mouse model of renal I/R we found that repetitive administration of p53 siRNA during the first 14 days after I/R reduced the senescence load and ameliorated the post-ischemic phenotype. Prolonged application of p53 siRNA over a 26 day period after I/R however, did not provide any additional benefit for senescence reduction, but reversed some of the renoprotective effects of the early treatment. These data suggest a time dependent role of p53 ...
Source: American Journal of Physiology. Renal Physiology - February 20, 2019 Category: Physiology Authors: Baisantry A, Berkenkamp B, Rong S, Bhayadia R, Sörensen-Zender I, Schmitt R, Melk A Tags: Am J Physiol Renal Physiol Source Type: research

Renoprotective effect of erythropoietin via modulation of the STAT6/MAPK/NF- κB pathway in ischemia/reperfusion injury after renal transplantation.
Renoprotective effect of erythropoietin via modulation of the STAT6/MAPK/NF-κB pathway in ischemia/reperfusion injury after renal transplantation. Int J Mol Med. 2017 Oct 20;: Authors: Zhang J, Zhao D, Na N, Li H, Miao B, Hong L, Huang Z Abstract Ischemia/reperfusion injury (IRI) commonly occurs in renal transplantation. Erythropoietin (EPO) exerts a protective effect in IRI. To investigate the underlying molecular mechanism, rat models of renal IRI were established and treated with EPO and/or lentivirus‑mediated EPO-siRNA, the signal transducer and activator of transcription 6 (STAT6) inhibito...
Source: International Journal of Molecular Medicine - October 20, 2017 Category: Molecular Biology Authors: Zhang J, Zhao D, Na N, Li H, Miao B, Hong L, Huang Z Tags: Int J Mol Med Source Type: research

Cell signalling
Conclusions: Taken together, it is likely that klotho protects against renal fibrotic process accompanied by down-regulation of fibrotic markers, counteracting to TGF-β, and one of possible mechanism mediating translocation of Na/K ATPase, resulting in Ca+ channel stabilization/alternation of Ca+ ion concentration. Klotho should be involved in the accentuation of the progression of renal fibrosis in CKD.
Source: Nephrology Dialysis Transplantation - May 10, 2013 Category: Urology & Nephrology Authors: Tsuchiya, K., Shiohira, S., Sugiura, H., Suzuki, M., Okano, K., Nitta, K., Kaesler, N., Immendorf, S., Ouyang, C., Carmeliet, P., Floege, J., Kruger, T., Schlieper, G., Georgescu, A., Kalucka, J., Olbrich, S., Baumgartl, J., Hackenbeck, T., Eckardt, K.-U. Tags: Abstracts Source Type: research

Cell signalling and apoptosis
Conclusions: These results suggest that activation of S1P signaling mediated by S1PR-3 results in chronic pathological fibrosis, such as in chronic kidney disease (CKD).
Source: Nephrology Dialysis Transplantation - May 21, 2014 Category: Urology & Nephrology Authors: Shiohira, S., Yoshida, T., Sugiura, H., Nishida, M., Nitta, K., Tsuchiya, K., Grampp, S., Goppelt-Strube, M., Eckardt, K. U., Schodel, J., Kang, S. W., Kim, Y., Seo, S.-K., Kim, T., Ong, S., Yang, W. S., Han, N. J., Lee, J. M., Baek, C. H., Park, S.-K., K Tags: Monday, June 2nd, 2014: Posters Source Type: research

Inhibition of autophagy increases apoptosis during re‐warming after cold storage in renal tubular epithelial cells
In conclusion, autophagy inhibition in RTECs after CS/REW induces apoptotic cell death and may be deleterious as a therapy to decrease DGF. This article is protected by copyright. All rights reserved.
Source: Transplant International - September 30, 2014 Category: Transplant Surgery Authors: Swati Jain, Daniel Keys, Trevor Nydam, Robert J. Plenter, Charles L. Edelstein, Alkesh Jani Tags: Original Article Source Type: research

Altered tooth morphogenesis after silencing the planar cell polarity core component, Vangl2
In this study, we show that Vangl2 is expressed in the inner dental epithelium (IDE) and in the secondary enamel knots (SEKs) of bell stage tooth germs. Inhibition of Vangl2 expression by siRNA treatment in in vitro-cultured tooth germs resulted in retarded tooth germ growth with deregulated cell proliferation and apoptosis. After kidney transplantation of Vangl2 siRNA-treated tooth germs, teeth were observed to be small and malformed. We also show that Vangl2 is required to maintain the proper pattern of cell alignment in SEKs, which maybe important for the function of SEKs as signaling centers. These results suggest that...
Source: Cell and Tissue Research - September 2, 2016 Category: Cytology Source Type: research

Essential role of microRNA-650 in the regulation of B-cell CLL/lymphoma 11B gene expression following transplantation: A novel mechanism behind the acute rejection of renal allografts.
Abstract Kidney transplantation is an effective final therapeutic procedure for patients with end-stage kidney failure. Although advanced immunosuppressive therapy is administered following transplantation, certain patients still suffer from acute allograft rejection. MicroRNAs (miRs) have a potential diagnostic and therapeutic value for acute renal allograft rejection; however, their underlying mechanism of action is largely unknown. In the present study, an increased level of miR-650 was identified to be associated with the downregulation of B-cell CLL/lymphoma 11B (BCL11B) expression in acute renal allograft ...
Source: International Journal of Molecular Medicine - October 17, 2017 Category: Molecular Biology Authors: Jin P, Chen H, Xie J, Zhou C, Zhu X Tags: Int J Mol Med Source Type: research