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Infectious Disease: Human Papillomavirus (HPV)

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Total 89 results found since Jan 2013.

Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention against head and neck cancer-containing HPV16 cell lines.
Conclusion: Our results indicate that siRNA E6 and/or E7 may have potential as a gene-specific therapy for human papillomavirus (HPV) type 16 (HPV16)-related squamous cell carcinoma of the head and neck (HNSCC). Objectives: To evaluate the effectiveness of siRNA targeting E6 and/or E7 on the in vitro and in vivo growth suppression of HPV16-related HNSCC. Methods: HPV16-related HNSCC (UM-SCC47) cell lines were used for the present study. Expression of HPV viral oncogenes E6 and/or E7 and their cellular targets, p53 and pRb, was evaluated by real-time PCR, Western blotting, and immunofluorescence staining. To study the effec...
Source: Acta Oto-Laryngologica - May 3, 2013 Category: ENT & OMF Authors: Adhim Z, Otsuki N, Kitamoto J, Morishita N, Kawabata M, Shirakawa T, Nibu KI Tags: Acta Otolaryngol Source Type: research

PEGylation of lipoplexes: The right balance between cytotoxicity and siRNA effectiveness.
In conclusion, the right balance between cytotoxicity and siRNA effectiveness has been found with the transfection of lipoplexes coated with 20% of Ceramide-PEG2000. This new nanovector could have a high potential against multiple mucosal diseases, such as human papillomavirus-induced genital lesions. PMID: 27593989 [PubMed - as supplied by publisher]
Source: European Journal of Pharmaceutical Sciences - August 31, 2016 Category: Drugs & Pharmacology Authors: Lechanteur A, Furst T, Evrard B, Delvenne P, Hubert P, Piel G Tags: Eur J Pharm Sci Source Type: research

The siRNA cocktail targeting interleukin 10 receptor and transforming growth factor‐β receptor on dendritic cells potentiates tumour antigen‐specific CD8+ T cell immunity
In this study, we used small interfering RNA (siRNA) to silence the expression of endogenous molecules in DCs, which can sense immunosuppressive factors. Among the siRNAs targeting various immunosuppressive molecules, we observed that DCs transfected with siRNA targeting IL‐10 receptor alpha (siIL‐10RA) initiated the strongest antigen‐specific CD8+ T cell immune responses. The potency of siIL‐10RA was enhanced further by combining it with siRNA targeting TGF‐β receptor (siTGF‐βR), which was the next best option during the screening of this study, or the previously selected immunoadjuvant siRNA targeting phosp...
Source: Clinical and Experimental Immunology - May 17, 2015 Category: Allergy & Immunology Authors: Y.‐H. Ahn, S.‐O. Hong, J. H. Kim, K. H. Noh, K.‐H. Song, Y.‐H. Lee, J.‐H. Jeon, D.‐W. Kim, J. H. Seo, T. W. Kim Tags: Original Article Source Type: research

Effects of siRNA-mediated suppression of HPV-11 L1 expression on the proliferation and apoptosis of vaginal epithelial cells.
Authors: Zeng J, Yang S, Wang X, Gao Y, Zhang M Abstract The aim of the present study was to investigate the effects of human papillomavirus (HPV) infection on the gynecological disease of vaginitis and to demonstrate how the small interfering RNA (siRNA) method may be used for HPV prevention in the clinic. Human vaginal epithelial cells were transfected with HPV-11 L1 expression vector and siRNA-HPV-11 L1 vectors and a control group was transfected with scrambled siRNA. Cell proliferation in each group was analyzed using the MTT assay and the expression of apoptosis-associated proteins was measured by western blot...
Source: Experimental and Therapeutic Medicine - April 18, 2017 Category: Journals (General) Tags: Exp Ther Med Source Type: research

Effect of HPV E6/E7 siRNA with Chemotherapeutic Agents on the Regulation of TP53/E2F Dynamic Behavior for Cell Fate Decisions.
Abstract Toxicity and resistance remain major challenges for advanced or recurrent cervical cancer therapies, as treatment requires high doses of chemotherapeutic agents. Restoration of TP53 and hypophosphorylated-retinoblastoma (pRB) proteins by human papillomavirus (HPV) E6/E7 siRNA sensitizes HPV-positive cervical cancer cells toward chemotherapeutic agents. Here, we investigated the therapeutic effects of E6/E7 siRNA on the dynamic behavior of TP53 and RB/E2F signaling networks in deciding the cell fate. The synergistic effect of HPV E6/E7 siRNA pool (SP) with chemotherapeutic agents on TP53 and RB/E2F signali...
Source: Neoplasia - August 23, 2017 Category: Cancer & Oncology Authors: Rajasekaran N, Jung HS, Bae SH, Chelakkot C, Hong S, Choi JS, Yim DS, Oh YK, Choi YL, Shin YK Tags: Neoplasia Source Type: research

Inhibition of SMYD2 Sensitized Cisplatin to Resistant Cells in NSCLC Through Activating p53 Pathway
In conclusion, the present study elucidated that the activity of SMYD2 in NSCLC may affect the cell sensitivity to chemotherapeutic agents, especially to CDDP. The elevated SMYD2 mediated CDDP resistance and malignant phenotype in NSCLC, indicating that SMYD2 may be a useful biomarker of CDDP resistance in NSCLC. Inhibition of SMYD2 contributes to the methylation-related activation of p53 and thus results in cell apoptosis. Furthermore, combination treatment with CDDP and an SMYD2 inhibitor had a synergistically antitumor effects in a xenograft model in vivo. Given that SMYD2 has reversible effects and is a targetable prot...
Source: Frontiers in Oncology - April 25, 2019 Category: Cancer & Oncology Source Type: research

Abstract 2115: Effect of small interfering RNA targeting HPV E6/E7 gene on the regulation of TP53/Rb dynamic behaviour in cervical cancer cells
Human papillomavirus (HPV) E6 and E7 viral oncogenes are very well known to cause cervical cancer, because E6 degrades TP53 tumor suppressor protein, and E7 inactivates the tumor suppressor retinoblastoma (pRb) protein. Thus E6 and E7 oncogenes of HPV are supposed to be promising targets of gene therapy against HPV mediated cervical cancer. Here, we attempted to study the regulation of TP53/pRb proteins dynamic behaviour after HPV E6/E7 small interfering RNA (siRNA) transfection in cervical cancer cells. HPV positive (HeLa and Caski) cell lines were selected for these experiments. Herein, we also validated the dynamics of ...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Rajasekaran, N., Jung, H. S., Kim, Y. D., Kim, D. A., Ha, T. K., Na, Y. H., Shin, Y. k. Tags: Molecular and Cellular Biology Source Type: research

IFIT5 Participates in the Antiviral Mechanisms of Rainbow Trout Red Blood Cells
We described a new defense mechanism based on IFIT5 antiviral activity in nucleated RBCs that appeared to contribute to halting the VHSV infection. This finding sheds light into novel antiviral therapeutics to mitigate the economic losses and social impact caused by viral infections in the aquaculture industry. This work broadens the knowledge of fish nucleated RBCs functions and their role in the immune response to viral infections. Ethics Statement Experimental protocols and methods relating to experimental animals were reviewed and approved by the Animal Welfare Body and the Research Ethics Committee at the University...
Source: Frontiers in Immunology - April 15, 2019 Category: Allergy & Immunology Source Type: research

Depletion of polycistronic transcripts using short interfering RNAs: cDNA synthesis method affects levels of non-targeted genes determined by quantitative PCR
Conclusions: These data emphasise the importance of the cDNA synthesis method used when measuring transcript abundance following siRNA depletion of polycistronic transcripts. They provide a partial explanation for erroneous reports suggesting that siRNAs targeting HPV E7 can have gene-specific effects.
Source: Virology Journal - May 21, 2013 Category: Virology Authors: Jennifer HanningIan GrovesMark PettNicholas Coleman Source Type: research

Cancerous inhibitor of protein phosphatase 2A contributes to human papillomavirus oncoprotein E7-induced cell proliferation via E2F1.
In this study, we demonstrated that HPV-16E7 protein significantly upregulating CIP2A mRNA and protein expression depended on retinoblastoma protein pRb rather than p130. CIP2A siRNA knockdown in HPV-E7-expressing cells inhibited cell proliferation, DNA synthesis and G1/S cell cycle progression. CIP2A siRNA decreased the protein levels of cyclin-dependent kinase 1 (Cdk1), Cdk2 and their partner cyclin A2, with no change in levels of Cdk4, Cdk6 and their partner cyclin D1. The downregulation of Cdk1 and Cdk2 was independent of c-Myc; instead, E2F1 was the main target of CIP2A in this process, as overexpression of E2F1 rescu...
Source: Oncotarget - February 6, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Short interfering RNA induced generation and translation of stable 5’ mRNA cleavage intermediates
Publication date: Available online 16 June 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago-2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the pos...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - June 16, 2016 Category: Genetics & Stem Cells Source Type: research

Short interfering RNA induced generation and translation of stable 5 ’ mRNA cleavage intermediates
Publication date: Available online 16 June 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago-2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the pos...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - June 17, 2016 Category: Genetics & Stem Cells Source Type: research

Short interfering RNA induced generation and translation of stable 5 ′ mRNA cleavage intermediates
Publication date: August 2016 Source:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Volume 1859, Issue 8 Author(s): Richa Singhania, Sandra Pavey, Elizabeth Payne, Wenyi Gu, Jennifer Clancy, Luqman Jubair, Thomas Preiss, Nicholas Saunders, Nigel A.J. McMillan Sequence-specific degradation of homologous mRNA is the main mechanism by which short-interfering RNAs (siRNAs) suppress gene expression. Generally, it is assumed that the mRNA fragments resulting from Ago2 cleavage are rapidly degraded, thus making the transcript translation-incompetent. However, the molecular mechanisms involved in the post-cleav...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - August 6, 2016 Category: Genetics & Stem Cells Source Type: research

Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes.
Abstract Deregulated expression of high-risk human papillomavirus oncogenes (E6 and E7) is a pivotal event for pathogenesis and progression in cervical cancer. Both viral oncogenes are therefore regarded as ideal therapeutic targets. Small interfering RNAs (siRNA) or double-stranded RNAs can knock down target genes effectively through siRNA-induced transcriptional gene silencing (TGS). Here, we established lentiviral-vector mediated shRNA (LV-shRNA) targeting common promoter of HPV16 E6/E7 and targeting E6 transcript, transduced the lentiviral construct into cervical HPV16-positive cell lines Siha and Caski, then ...
Source: Antiviral Research - May 1, 2013 Category: Virology Authors: Zhou J, Li B, Peng C, Wang F, Fu Z, Zhou C, Hong D, Ye F, Lü W, Xie X Tags: Antiviral Res Source Type: research

Deregulation of microRNAs Let-7a and miR-21 mediate aberrant STAT3 signaling during human papillomavirus-induced cervical carcinogenesis: role of E6 oncoprotein
Conclusions: Our results demonstrate existence of a functional loop involving Let-7a, STAT3 and miR-21 which were found potentially regulated by viral oncoprotein E6. Implications: miR-21 and Let-7a along with STAT3 may prove useful targets for pharmacological intervention for management of cervical cancer.
Source: BMC Cancer - December 23, 2014 Category: Cancer & Oncology Authors: Gauri ShishodiaGaurav VermaYogesh SrivastavaRavi MehrotraBhudev DasAlok Bharti Source Type: research