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Condition: Inflammatory Bowel Disease

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Total 147 results found since Jan 2013.

Effects of particle size and binding affinity for siRNA on the cellular processing, intestinal permeation, and anti‐inflammatory efficacy of polymeric nanoparticles
ConclusionNanoparticles with particle size of 450 nm or more balanced binding affinity for siRNA might be preferable for the treatment of ulcerative colitis. This article is protected by copyright. All rights reserved.
Source: The Journal of Gene Medicine - August 9, 2015 Category: Genetics & Stem Cells Authors: Wenyue Cheng, Cui Tang, Chunhua Yin Tags: Research Article Source Type: research

Systemic delivery of siRNA to the colon using peptide modified PEG-PCL polymer micelles for the treatment of ulcerative colitis
In this study, we investigated the effects of siRNA delivered using MPEG-PCL-CH2R4H2C nanomicelles through intravenous administration to the inflammation site of dextran sulfate sodium-induced colitis mice. The MPEG-PCL-CH2R4H2C micelles had optimum physical properties and high siRNA compaction ability. Moreover, model-siRNA delivered through MPEG-PCL-CH2R4H2C showed higher accumulation in the inflammatory site than that of the naked siRNA. Furthermore, intravenous administration of MPEG-PCL-CH2R4H2C/siRelA micelles, targeting siRelA, a subunit of NF-κB, significantly decreased the shortening of large intestine, clinical ...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 29, 2021 Category: Drugs & Pharmacology Authors: Hisako Ibaraki Naruhiro Hatakeyama Naoki Arima Akihiro Takeda Yasuo Seta Takanori Kanazawa Source Type: research

siRNA-based nanotherapeutics as emerging modalities for immune-mediated diseases: A preliminary review
This study will pave the way for new avenues of research into the diagnosis and treatment of IMDs.PMID:35830711 | DOI:10.1002/cbin.11841
Source: Cell Biology International - July 13, 2022 Category: Cytology Authors: Saman Sargazi Rabia Arshad Reza Ghamari Abbas Rahdar Ali Bakhshi Sonia Fathi Karkan Narges Ajalli Muhammad Bilal Ana M D íez-Pascual Source Type: research

Enzyme- and pH-responsive microencapsulated nanogels for oral delivery of siRNA to induce TNF-α knockdown in the intestine.
Abstract Inflammatory bowel diseases (IBD) manifest from excessive intestinal inflammation. Local delivery of siRNA that targets these inflammatory cytokines would provide a novel treatment approach. Microencapsulated nanogels are designed and validated as platforms for oral delivery of siRNA targeting TNF-α, a common clinical target of IBD treatments. The preferred platform was designed to (i) protect siRNA-loaded nanogels from the harsh acidic environment of the upper GI tract; and (ii) enzymatically degrade and release the nanogels once the carrier has reached the intestinal region. This platform consists of m...
Source: Biomacromolecules - January 26, 2016 Category: Biochemistry Authors: Knipe JM, Strong LE, Peppas NA Tags: Biomacromolecules Source Type: research

Gemini lipid nanoparticle (GLNP)-mediated oral delivery of TNF- α siRNA mitigates gut inflammation < em > via < /em > inhibiting the differentiation of CD4 < sup > + < /sup > T cells
Nanoscale. 2022 Sep 28. doi: 10.1039/d1nr05644a. Online ahead of print.ABSTRACTProinflammatory cytokines such as Tumor Necrosis Factor-α (TNF-α) are critical mediators of inflammatory bowel disease pathogenesis, and are important targets to restore intestinal homeostasis. Herein, we present the engineering and screening of gemini lipid nanoparticles (GLNPs) for siRNA delivery to colon epithelial cells, macrophages and dendritic cells, and their ability to deliver siRNA therapeutics to the inflamed gastrointestinal tract. We synthesized eight gemini cationic lipids by tethering two lithocholic acid molecules through 3'-hy...
Source: Cell Research - September 28, 2022 Category: Cytology Authors: Priyanka Verma Amit Arora Kajal Rana Devashish Mehta Raunak Kar Vikas Verma C V Srikanth Veena S Patil Avinash Bajaj Source Type: research

A Genome-Wide siRNA Screen Reveals Positive and Negative Regulators of the NOD2 and NF-κB Signaling Pathways.
Abstract The cytoplasmic receptor NOD2 (nucleotide-binding oligomerization domain 2) senses peptidoglycan fragments and triggers host defense pathways, including activation of nuclear factor κB (NF-κB) signaling, which lead to inflammatory immune responses. Dysregulation of NOD2 signaling is associated with inflammatory diseases, such as Crohn's disease and Blau syndrome. We used a genome-wide small interfering RNA screen to identify regulators of the NOD2 signaling pathway. Several genes associated with Crohn's disease risk were identified in the screen. A comparison of candidates from this screen with other "o...
Source: Science Signaling - January 23, 2013 Category: Biomedical Science Authors: Warner N, Burberry A, Franchi L, Kim YG, McDonald C, Sartor MA, Núñez G Tags: Sci Signal Source Type: research

4 co-delivery of tnf- α sirna and il-22 via a nanoparticle-in-hydrogel system exerts synergistic therapeutic effects against ulcerative colitis
Combination therapy is an emerging approach for treating ulcerative colitis (UC). During the development of UC, TNF- α acts as the major pro-inflammatory cytokine while interleukin-22 (IL-22) plays an important role in preventing mucosal damage. In the present study, we loaded TNF-α siRNA (siTNF) into galactosylated polymeric nanoparticles (NPs). The resultant Gal-siTNF-NPs had a desirable diameter (～261 nm), narrow size distribution and a slightly negative zeta potential (～‒12 mV). These NPs successful mediated the targeted delivery of siTNF to macrophages and efficiently inhibited the expression of TNF-α.
Source: Gastroenterology - January 1, 2018 Category: Gastroenterology Authors: Bo Xiao, Emilie Viennois, Tim L. Denning, Didier Merlin Tags: Clinical and Research Challenges Source Type: research

siRNA-based identification of IBD-related targets in human monocyte-derived dendritic cells
J Immunol Methods. 2021 Apr 20:113058. doi: 10.1016/j.jim.2021.113058. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) is thought to be caused by an aberrant host response to the commensal enteric flora in genetically susceptible individuals. Dendritic cells (DCs) play a key role in the regulation of this response as they sample gut commensals. In healthy individuals DCs actively contribute to tolerance upon recognition of these resident bacteria, whereas in individuals with IBD, DCs will initiate an inflammatory response. To mimic the disease response in vitro, human monocyte-derived DCs were matured with E...
Source: Journal of Immunological Methods - April 23, 2021 Category: Allergy & Immunology Authors: Diane Bosman-Schluep Remko de Pril Banu Verbaken Ana ïs Legent Jan Stallen Esther C de Jong Richard A J Janssen Source Type: research

Effects of particle size and binding affinity for small interfering RNA on the cellular processing, intestinal permeation and anti‐inflammatory efficacy of polymeric nanoparticles
ConclusionsNanoparticles with a particle size of 450 nm or balanced binding affinity for siRNA might be preferable for the treatment of ulcerative colitis. Copyright © 2015 John Wiley & Sons, Ltd.
Source: The Journal of Gene Medicine - December 11, 2015 Category: Genetics & Stem Cells Authors: Wenyue Cheng, Cui Tang, Chunhua Yin Tags: Research Article Source Type: research

Auranofin, an Anti-rheumatic Gold Drug, Aggravates the Radiation-Induced Acute Intestinal Injury in Mice
Conclusion In this study, we found that a non-toxic dose of auranofin significantly aggravated the severity of the radiation-induced intestinal injury. This suggests that auranofin treatment can be an independent factor that influences the risk of intestinal complications after pelvic or abdominal radiotherapy. Ethics Statement All the protocols used in this study were approved by the Institutional Animal Care and Use Committee of the Korean Institute of Radiological and Medical Sciences (IACUC permit number: KIRAMS217-0007). Author Contributions H-JL, JS, and Y-BL designed the experiments. EL and JK conducted the exp...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research