Filtered By:
Condition: Anxiety
This page shows you your search results in order of relevance.
Order by Relevance | Date
Total 25 results found since Jan 2013.
i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of Neuromics' Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news
i-Fect Delivers siRNA in vitro and in vivo
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: Neuromics - February 21, 2015 Category: Neuroscience Tags: Cerebral Ischemia Connexin 43 Delivering siRNA to the CNS EPO HDAC2 i-Fect Stroke Source Type: news
HDAC2 and Anxiety in Alcoholism
The Impact of HDAC2 Gene Expression on AnxietyOur i-Fect Transfection Kit continues to be a potent tool for testing the impact of altered gene expression on behavior. see: SACHIN MOONAT. The Role of Amygdaloid Chromatin and Synaptic Remodeling in Anxiety and Alcoholism. THESIS Submitted as partial fulfillment of the requirements for the degree of Doctor of Philosophy in Neuroscience in the Graduate College of the University of Illinois at Chicago, 2014.The author hypothesized that increased HDAC2 would have a positive impact on anxiety in alchohol preferring (P) rats. Specifically, HDAC2-induced histone modifications ...
Source: siRNA and DsiRNA Transfection Efficiency - November 24, 2014 Category: Neuroscience Tags: Alcoholism Anxiety BDNF Gene Expression Analysis Gene Silencing HDAC2 gene Learning Source Type: news
i-Fect Delivers Again!
Knocks Down Suspected Stress/Anxiety ReceptorThe molecular pathogenesis underlying anxiety disorders is still unclear. Here, the authors demonstrate that myristoylated alanine-rich C-kinase substrate like 1 (MARCKSL1) overexpression in mice increases spine formation in the amygdala and induces stress hormone upregulation and anxiety-like behaviors. Suppression of MARCKSL1 in the amygdala ameliorates both the increase in stress hormones and the elevated anxiety-like behaviors. Our results indicate that MARCKSL1 expression in the amygdala plays an important role in anxiety-like behaviors.This was proved, in part, by the knoc...
Source: siRNA and DsiRNA Transfection Efficiency - March 28, 2018 Category: Neuroscience Tags: Amygdala Anxiety i-Fect MARCKSL1 Stress Source Type: news
Unmasking Root Causes of Stress/Anxiety
i-Fect Knocks Down Suspected Stress/Anxiety ReceptorThe molecular pathogenesis underlying anxiety disorders is still unclear. Here, the authors demonstrate that myristoylated alanine-rich C-kinase substrate like 1 (MARCKSL1) overexpression in mice increases spine formation in the amygdala and induces stress hormone upregulation and anxiety-like behaviors. Suppression of MARCKSL1 in the amygdala ameliorates both the increase in stress hormones and the elevated anxiety-like behaviors. Our results indicate that MARCKSL1 expression in the amygdala plays an important role in anxiety-like behaviors.This was proved, in part, by t...
Source: Neuromics - March 29, 2018 Category: Neuroscience Tags: Anxiety gene expression analysis i-Fect MARCKSL1 Source Type: news
Complexity of Compensatory Effects in Nrf1 Knockdown: Linking Undeveloped Anxiety-Like Behavior to Prevented Mitochondrial Dysfunction and Oxidative Stress.
Abstract
Anxiety-related disorders are complex illnesses that underlying molecular mechanisms need to be understood. Mitochondria stand as an important link between energy metabolism, oxidative stress, and anxiety. The nuclear factor, erythroid-derived 2,-like 1(Nrf1) is a member of the cap "n" collar subfamily of basic region leucine zipper transcription factors and plays the major role in regulating the adaptive response to oxidants and electrophiles within the cell. Here, we injected small interfering RNA (siRNA) targeting Nrf1 in dorsal third ventricle of adult male albino Wistar rats and subsequently examined...
Source: Cellular and Molecular Neurobiology - July 23, 2015 Category: Cytology Authors: Khalifeh S, Oryan S, Khodagholi F, Digaleh H, Shaerzadeh F, Maghsoudi N, Zarrindast MR Tags: Cell Mol Neurobiol Source Type: research
LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway.
Abstract
Atrazine (2-chloro-4-ethylamino-6-isopropylamine-1,3,5-triazine; ATR) has been demonstrated to regulate autophagy- and apoptosis-related proteins in doparminergic neuronal damage. In our study, we investigated the role of LC3-II in ATR-induced degeneration of dopaminergic neurons. In vivo dopaminergic neuron degeneration model was set up with ATR treatment and confirmed by the behavioral responses and pathological analysis. Dopaminergic neuron cells were transfected with LC3-II siRNA and treated with ATR to observe cell survival and reactive oxygen species release. The process of mitochondrial autophagy a...
Source: Acta Biochimica et Biophysica Sinica - August 2, 2018 Category: Biochemistry Authors: Ma K, Wu H, Li P, Li B Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research
mGlu7 VFTD-directed Antagonist Relieves Fear and Stress Signal Transduction
The metabotropic glutamate receptor subtype 7 (mGlu7) is an important presynaptic regulator of neurotransmission in the mammalian CNS. mGlu7 function has been linked to autism, drug abuse, anxiety, and depression. Despite this, it has been difficult to develop specific blockers of native mGlu7 signaling in relevant brain areas such as amygdala and limbic cortex. Here, we present the mGlu7-selective antagonist 7-hydroxy-3-(4-iodophenoxy)-4H-chromen-4-one (XAP044), which inhibits lateral amygdala long term potentiation (LTP) in brain slices from wild type mice with a half-maximal blockade at 88 nm. There was no effect of XAP...
Source: Journal of Biological Chemistry - April 17, 2014 Category: Chemistry Authors: Gee, C. E., Peterlik, D., Neuhauser, C., Bouhelal, R., Kaupmann, K., Laue, G., Uschold–Schmidt, N., Feuerbach, D., Zimmermann, K., Ofner, S., Cryan, J. F., van der Putten, H., Fendt, M., Vranesic, I., Glatthar, R., Flor, P. J. Tags: Neurobiology Source Type: research
Involvement of Nrf2 in Development of Anxiety-Like Behavior by Linking Bcl2 to Oxidative Phosphorylation: Estimation in Rat Hippocampus, Amygdala, and Prefrontal Cortex
In this study, we evaluated whether silencing of Nrf2 plays a role in development of anxiety-related behavior. In this regard, we exerted small interfering RNA (siRNA) targeting Nrf2 in dorsal third ventricle and subsequently examined the effect of this silencing on anxiety-related behavior along with supposed molecular mechanisms. Therefore, we evaluated apoptotic markers and mitochondrial electron transport chain (ETC) activity in three brain regions: hippocampus, amygdala, and prefrontal cortex. Based on our result, Nrf2-silenced rats exhibited greater anxiety-like behavior compared to control group. Furthermore, Nrf2 s...
Source: Journal of Molecular Neuroscience - January 23, 2015 Category: Neuroscience Source Type: research
Antidepressants activate the lysophosphatidic acid receptor LPA1 to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 fibroblasts.
Abstract
Different lines of evidence indicate that the lysophosphatidic acid (LPA) receptor LPA1 is involved in neurogenesis, synaptic plasticity and anxiety-related behaviour, but little is known on whether this receptor can be targeted by neuropsychopharmacological agents. The present study investigated the effects of different antidepressants on LPA1 signaling. We found that in Chinese hamster ovary (CHO)-K1 fibroblasts expressing endogenous LPA1 tricyclic and tetracyclic antidepressants and fluoxetine induced the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and CREB. This response was a...
Source: Biochemical Pharmacology - April 15, 2015 Category: Drugs & Pharmacology Authors: Olianas MC, Dedoni S, Onali P Tags: Biochem Pharmacol Source Type: research
Role of cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) in regulating the trafficking and signaling of corticotropin-releasing factor receptor 1.
Abstract
Corticotropin releasing factor (CRF) receptor1 (CRFR1) is associated with psychiatric illness and is a proposed target for the treatment of anxiety and depression. Like many G protein-coupled receptors (GPCRs), CRFR1 harbors a PDZ (PSD-95/Disc Large/Zona Occludens 1)-binding motif at the end of its carboxyl terminal tail. The interactions of PDZ proteins with GPCRs is crucial for the regulation of their receptor function. In the present study, we characterize the interaction of the cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) with CRFR1. We show using co-immunoprecipitation ...
Source: Cellular Signalling - June 23, 2015 Category: Cytology Authors: Hammad MM, Dunn HA, Walther C, Ferguson SS Tags: Cell Signal Source Type: research
BDNFVal66met polymorphism: a potential bridge between depression and thrombosis
Conclusion</div>Activation of platelets, alteration in coagulation pathways, and changes in vessel wall protein expression in BDNF<sup>Met/Met</sup> mice recapitulate well the features occurring in the anxiety/depression condition. Furthermore, our data suggest that the BDNFVal66Met polymorphism contribute to the individual propensity for arterial thrombosis related to AMI.</span>
Source: European Heart Journal - December 26, 2015 Category: Cardiology Source Type: research
Amygdalar expression of the microRNA miR ‐101a and its target Ezh2 contribute to rodent anxiety‐like behavior
This article is protected by copyright. All rights reserved.
Source: European Journal of Neuroscience - June 14, 2017 Category: Neuroscience Authors: Joshua L. Cohen, Nateka L. Jackson, Mary E. Ballestas, William M. Webb, Farah D. Lubin, Sarah M. Clinton Tags: Research Report Source Type: research
30 YEARS OF THE MINERALOCORTICOID RECEPTOR: Mineralocorticoid receptor null mice: informing cell-type-specific roles
The mineralocorticoid receptor (MR) mediates the actions of two important adrenal corticosteroid hormones, aldosterone and cortisol. The cell signalling roles of the MR in vivo have expanded enormously since the cloning of human MR gene 30 years ago and the first MR gene knockout in mice nearly 20 years ago. Complete ablation of the MR revealed important roles postnatally for regulation of kidney epithelial functions, with MR-null mice dying 1–2 weeks postnatally from renal salt wasting and hyperkalaemia, with elevated plasma renin and aldosterone. Generation of tissue-selective MR-deficient mice using Cre recombinas...
Source: Journal of Endocrinology - June 20, 2017 Category: Endocrinology Authors: Cole, T. J., Young, M. J. Tags: Thematic Review Source Type: research
A role for Galanin N-terminal fragment (1-15) In anxiety and depression in rats
Galanin (GAL) is involved in several functions including mood regulation. The GAL N-terminal fragment (1-15) [GAL(1-15)] also participates at central level and a differential role of GAL(1-15) compared with GAL has been proposed. In this work we have analysed if GAL(1-15) contributes to depression- and anxiety -related behaviours using the forced swimming test, tail suspension test, open field and light/dark test. We tested the involvement of the GAL receptor 2 (GALR2) in GAL(1-15) effects with the GAL receptor antagonist M871 and with an in vivo model of siRNA GALR2 knockdown rats.
Source: Neuropeptides - October 1, 2017 Category: Neuroscience Authors: Carmelo Mill ón, Antonio Flores-Burguess, Manuel Narváez, Dasiel Borroto-Escuela, Luis Santín, Jose Angel Narváez, Kjell Fuxe, Zaida Díaz-Cabiale Source Type: research
