Filtered By:
Infectious Disease: Herpes

This page shows you your search results in order of date. This is page number 6.

Order by Relevance | Date

Total 89 results found since Jan 2013.

Epstein-Barr virus glycoprotein gM can interact with the cellular protein p32 and knockdown of p32 impairs virus.
We report here that the long predicted cytoplasmic tail of gM is not required for complex formation and that it interacts with the cellular protein p32, which has been reported to be involved in nuclear egress of human cytomegalovirus and herpes simplex virus. Although redistribution of p32 and colocalization with gM was not observed in virus infected cells, knockdown of p32 expression by siRNA or lentivirus-delivered shRNA recapitulated the phenotype of a virus lacking expression of gNgM. A proportion of virus released from cells sedimented with characteristics of virus lacking an intact envelope and there was an increase...
Source: Virology - January 13, 2016 Category: Virology Authors: Changotra H, Turk SM, Artigues A, Thakur N, Gore M, Muggeridge MI, Hutt-Fletcher LM Tags: Virology Source Type: research

Efficient Gene Suppression in Dorsal Root Ganglia and Spinal Cord Using Adeno-Associated Virus Vectors Encoding Short-Hairpin RNA.
Abstract RNA interference is a powerful tool used to induce loss-of-function phenotypes through post-transcriptional gene silencing. Small interfering RNA (siRNA) molecules have been used to target the central nervous system (CNS) and are expected to have clinical utility against refractory neurodegenerative diseases. However, siRNA is characterized by low transduction efficiency, insufficient inhibition of gene expression, and short duration of therapeutic effects, and is thus not ideal for treatment of neural tissues and diseases. To address these problems, viral delivery of short-hairpin RNA (shRNA) expression ...
Source: Herpes - October 18, 2015 Category: Infectious Diseases Authors: Enomoto M, Hirai T, Kaburagi H, Yokota T Tags: Methods Mol Biol Source Type: research

Herpes Virus Entry Mediator in Human Corneal Epithelial Cells Modulates the Production of Inflammatory Cytokines in Response to HSV Type 1 Challenge
Conclusions: HVEM in human corneal epithelial cells may act to dampen the production of some cytokines and chemokines and thus it may modulate the innate immune response against HSV-1. This may provide a novel mechanism for the pathogenesis of HSV-1 infection in the cornea.Ophthalmic Res 2015;54:128-134
Source: Ophthalmic Research - August 31, 2015 Category: Opthalmology Source Type: research

Short nucleotide sequences in herpesviral genomes identical to the human DNA
Publication date: 7 May 2015 Source:Journal of Theoretical Biology, Volume 372 Author(s): Felix Filatov , Alexander Shargunov In 2010, we described many similar DNA sequences in human and viral genomes, including herpesviral ones. The data obtained allowed us to suggest that these motifs may provide the antiviral protection by mating with a complementary potential target and destroying it by the catalytic way like small interfering RNA, siRNA. Since we have analyzed these viruses as a group, two major issues seemed to us curious: (1) the number of such motifs in genomes of various herpesvirus types, and (2) distribution ...
Source: Journal of Theoretical Biology - March 12, 2015 Category: Biology Source Type: research

Early nucleosome deposition on, and replication of, HSV DNA requires cell factor PCNA
In this study, we examine the role of proliferating cell nuclear antigen (PCNA), a cellular DNA polymerase accessory protein (processivity factor), and cell DNA polymerases in histone deposition during the early stages of HSV infection. Using SiRNA knockdown, and a cytosine arabinoside (araC) chemical inhibitor, we conclude that PCNA is important for viral replication and histone deposition. However, cell DNA polymerases that bind PCNA do not appear to be required for these processes and PCNA does not appear to bind to the viral DNA polymerase (which has its own viral processivity factor).
Source: Journal of NeuroVirology - February 12, 2015 Category: Neurology Source Type: research

CRF-amplified neuronal TLR4/MCP-1 signaling regulates alcohol self-administration.
We report that alcohol-preferring P rats have innately elevated levels of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein-1 (MCP-1) that co-localize in neurons from the CeA and VTA. To examine the potential role of a TLR4/MCP-1 signal, we used Herpes Simplex Virus (HSV) vectors (amplicons) that retain in vivo neurotropism. Infusion of amplicons for TLR4 or MCP-1 siRNA into the CeA or VTA from the P rats inhibited target gene expression and blunted binge drinking. A similarly delivered amplicon for scrambled siRNA did not inhibit TLR4 or MCP-1 expression nor reduce binge drinking, identifying a neuronal TLR4/MC...
Source: Herpes - January 8, 2015 Category: Infectious Diseases Authors: June HL, Liu J, Warnock KT, Bell KA, Balan I, Bollino D, Puche A, Aurelian L Tags: Neuropsychopharmacology Source Type: research

Silencing sexually transmitted infections: topical siRNA-based interventions for the prevention of HIV and HSV.
Authors: Wheeler LA Abstract The global impact of sexually transmitted infections (STIs) is significant. The sexual transmission of viruses such as herpes simplex virus type-2 (HSV-2) and the human immunodeficiency virus type-1 (HIV-1), has been especially difficult to control. To date, no effective vaccines have been developed to prevent the transmission of these STIs. Although antiretroviral drugs have been remarkably successful in treating the symptoms associated with these viral infections, the feasibility of their widespread use for prevention purposes may be more limited. Microbicides might provide an attract...
Source: Infectious Diseases in Obstetrics and Gynecology - November 18, 2014 Category: OBGYN Tags: Infect Dis Obstet Gynecol Source Type: research

A New Approach of Delivering siRNA to the Cornea and its Application for Inhibiting Herpes Simplex Keratitis.
Abstract Small interfering RNA (siRNA) is a potential agent for the treatment of ocular surface diseases. Previous studies delivered siRNA by directly injecting siRNA into cornea or conjunctiva. In the present study we sought to explore an alternative approach to deliver siRNA into mouse cornea via eye drops that contains cy3-labeled siRNA (cy3-siRNA) and different cationic complexing agents and to evaluate the effects of siRNA targeting HSV-1 ICP4 gene (ICP4-siRNA) on mouse herpes simplex keratitis (HSK). Cy3-siRNA was mixed with Lipofectamine 2000, EntransterTM-in vivo, polyethyleneimine (PEI) or PEO-PPO-PEO pol...
Source: Current Molecular Medicine - October 21, 2014 Category: Molecular Biology Authors: Li Z, Duan F, Lin L, Huang Q, Wu K Tags: Curr Mol Med Source Type: research

Innate responses to small interfering RNA pools inhibiting herpes simplex virus infection in astrocytoid and epithelial cells.
Abstract Herpes simplex virus (HSV) is a human pathogen that can cause severe diseases such as encephalitis, keratitis and neonatal herpes. Control of HSV infection may be achieved by using small interfering (si)RNAs. We have designed and enzymatically produced pools of siRNAs targeting HSV. In addition to the target-specific effects, such siRNAs may induce innate immunity responses that may contribute to antiviral effects. HSV has versatile ways of modulating innate immunity, and it remains unclear whether HSV-specific antiviral treatment would benefit from the potential immunostimulatory effects of siRNAs. To ad...
Source: Innate Immunity - July 3, 2014 Category: Allergy & Immunology Authors: Paavilainen H, Romanovskaya A, Nygårdas M, Bamford DH, Poranen MM, Hukkanen V Tags: Innate Immun Source Type: research

Heat-shock protein 90 promotes nuclear transport of herpes simplex virus 1 capsid protein by interacting with acetylated tubulin.
In this study, we found that Hsp90 inhibitors have potent antiviral activity against standard or drug-resistant HSV-1 strains and viral gene and protein synthesis are inhibited in an early phase. More detailed studies demonstrated that Hsp90 is upregulated by virus entry and it interacts with virus. Hsp90 knockdown by siRNA or treatment with Hsp90 inhibitors significantly inhibited the nuclear transport of viral capsid protein (ICP5) at the early stage of HSV-1 infection. In contrast, overexpression of Hsp90 restored the nuclear transport that was prevented by the Hsp90 inhibitors, suggesting that Hsp90 is required for nuc...
Source: Herpes - June 8, 2014 Category: Infectious Diseases Authors: Zhong M, Zheng K, Chen M, Xiang Y, Jin F, Ma K, Qiu X, Wang Q, Peng T, Kitazato K, Wang Y Tags: PLoS One Source Type: research

RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions.
Abstract The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large subunit of ribonucleotide reductase, an essential enzyme for DNA synthesis. Herpes simplex virus type-1 was isolated from saliva samples and mucocutaneous lesions from infected patients. All mucocutaneous lesions' samples were positive for herpes simple...
Source: Braz J Infect Dis - May 15, 2014 Category: Infectious Diseases Authors: da Silva AP, Lopes JF, de Paula VS Tags: Braz J Infect Dis Source Type: research

Consequences of HSV-1-induced AS1 deficiency Microbiology
Herpes simplex virus 1 (HSV-1) infection triggers specific metabolic changes in its host cell. To explore the interactions between cellular metabolism and HSV-1 infection, we performed an siRNA screen of cellular metabolic genes, measuring their effect on viral replication. The screen identified multiple enzymes predicted to influence HSV-1 replication, including...
Source: Proceedings of the National Academy of Sciences - December 17, 2013 Category: Science Authors: Grady, S. L., Purdy, J. G., Rabinowitz, J. D., Shenk, T. Tags: PNAS Plus Source Type: research

Rab6 Dependent Post‐Golgi Trafficking of HSV1 Envelope Proteins to Sites of Virus Envelopment
Abstract Herpes simplex virus 1 (HSV1) is an enveloped virus that uses undefined transport carriers for trafficking of its glycoproteins to envelopment sites. Screening of an siRNA library against 60 Rab GTPases revealed Rab6 as the principal Rab involved in HSV1 infection, with its depletion preventing Golgi‐to‐plasma membrane transport of HSV1 glycoproteins in a pathway used by several integral membrane proteins but not the luminal secreted protein Gaussia luciferase. Knockdown of Rab6 reduced virus yield to 1% and inhibited capsid envelopment, revealing glycoprotein exocytosis as a prerequisite for morphogenesis. Ra...
Source: Traffic - October 23, 2013 Category: Research Authors: Helen L. Johns, Claudia Gonzalez‐Lopez, Charlotte L. Sayers, Michael Hollinshead, Gillian Elliott Tags: Original Article Source Type: research

Herpesviruses and the microbiome
The focus of this article will be to examine the role of common herpesviruses as a component of the microbiome of atopic patients and to review clinical observations suggesting that atopic patients might be predisposed to more severe and atypical herpes-related illness because their immune response is biased toward a TH2 cytokine profile. Human populations are infected with 8 herpesviruses, including herpes simplex virus HSV1 and HSV2 (also termed HHV1 and HHV2), varicella zoster virus (VZV or HHV3), EBV (HHV4), cytomegalovirus (HHV5), HHV6, HHV7, and Kaposi sarcoma–associated herpesvirus (termed KSV or HHV8). Herpesviru...
Source: Journal of Allergy and Clinical Immunology - April 22, 2013 Category: Allergy & Immunology Authors: David H. Dreyfus Tags: Rostrum Source Type: research