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Total 442 results found since Jan 2013.

PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart.
Abstract The remarkable regenerative capacity of the zebrafish has made it an important model organism for studying heart regeneration. However, current loss-of-function studies are limited by a lack of conditional-knockout and effective gene-knockdown methods for the adult heart. Here, we report a novel siRNA knockdown method facilitated by poly (ethylene glycol)-b-poly (D, l-lactide) (PEG-PLA) nanoparticles. The siRNA-encapsulated nanoparticles successfully entered cells and resulted in remarkable gene-specific knockdown in the adult heart. This effect was demonstrated by down-regulation of the Aldh1a2 and Dusp6...
Source: Developmental Biology - August 29, 2015 Category: Biology Authors: Diao J, Wang H, Chang N, Zhou XH, Zhu X, Wang J, Xiong JW Tags: Dev Biol Source Type: research

Specific Delivery of Delta-5-Desaturase siRNA via RNA Nanoparticles Supplemented with Dihomo-γ-Linolenic Acid for Colon Cancer Suppression
In this study, we employed RNA nanotechnology for specific delivery of D5D-siRNA to xenograft colon tumors using 3WJ RNA nanoparticles. When a targeting module, i.e., the EpCAM aptamer, was incorporated, the 3WJ pRNA nanoparticles were able specifically deliver D5D siRNA to human colon cancer HCA-7 cells both in vitro and in vivo, resulting in significant downregulation of D5D expression. Co-treatment with DGLA in combination with 3WJ-EpCAM-siRNA induced a higher DGLA/AA ratio and enhanced formation of 8-HOA at a threshold level, and in HCA-7 tumor-bearing mice, induced significant tumor suppression. We further confirmed t...
Source: Redox Biology - December 19, 2018 Category: Biology Source Type: research

Effect of siRNA PERK on Fluoride-Induced Osteoblastic Differentiation in OS732 Cells.
This study proved that the mechanism underlying fluoride induced osteoblastic and osteoclastic differentiation possible was due to activation of ALP and RANKL mediated by PERK in OS732 cells. PMID: 24838929 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - May 18, 2014 Category: Biology Authors: Lü P, Li X, Ruan L, Xu H, Liu Q Tags: Biol Trace Elem Res Source Type: research

siRNA transfection in the barnacle Amphibalanus amphitrite larvae METHODS amp TECHNIQUES
In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either nauplius or cyprid stage, or both. Effects of siRNA transfection on p38 MAPK level were hardly detectable in the cyprids that their corresponding nauplii were transfected. In contrast, larvae that were transfected at cyprid stage showed lower level of p38 MAPK than the blank and reagent controls. However, significantly decreased level of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rate were observed only in the "Double Transfection", in which larvae were exposed to siRNA solution at both the naupliu...
Source: Journal of Experimental Biology - June 25, 2015 Category: Biology Authors: Zhang, G., He, L.-s., Wong, Y. H., Yu, L., Qian, P.-y. Tags: METHODS [amp ] TECHNIQUES Source Type: research

siRNA transfection in larvae of the barnacle Amphibalanus amphitrite METHODS amp TECHNIQUES
In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in ‘double transfections’, in which larvae were exposed to s...
Source: Journal of Experimental Biology - August 19, 2015 Category: Biology Authors: Zhang, G., He, L.-S., Wong, Y. H., Yu, L., Qian, P.-Y. Tags: METHODS [amp ] TECHNIQUES Source Type: research

siRNA ‐loaded liposomes: inhibition of encystment of Acanthamoeba and toxicity on the eye surface
This article is protected by copyright. All rights reserved. Glycogen phosphorilase, a key enzyme for encystment of Acanthamoeba,was silenced PEGylated cationic liposomes were used as carriers for siRNA delivery Ther was a significant reduction in the encystment of AP2 trophozoties
Source: Chemical Biology and Drug Design - January 29, 2017 Category: Biology Authors: Kathrin Faber, Giovanni K. Zorzi, Nathaly T. Brazil, Marilise B. Rott, Helder F. Teixeira Tags: Research Article Source Type: research

TLR4 siRNA inhibits proliferation and invasion in colorectal cancer cells by downregulating ACAT1 expression
Publication date: Available online 10 May 2016 Source:Life Sciences Author(s): Kai Ye, Yiyang Wu, Yafeng Sun, Jian'an Lin, Jianhua Xu Aims Toll-like receptor 4 (TLR4) is involved in tumor development. Numerous studies have confirmed that TLR4 mediates processes in tumorigenesis, for example, inflammation, proliferation and invasion. However, the effects of TLR4 on colorectal cancer development have not been fully elucidated. The present study aimed to evaluate the effects and mechanisms of TLR4 on colorectal cancer development. Main methods The expression of TLR4 and Acyl coenzyme A cholesterol acyltransferase 1 (A...
Source: Life Sciences - May 10, 2016 Category: Biology Source Type: research

Application of bifurcation theory and siRNA-based control signal to restore the proper response of cancer cells to DNA damage
Publication date: 7 November 2016 Source:Journal of Theoretical Biology, Volume 408 Author(s): Emilia Kozłowska, Krzysztof Puszynski Many diseases with a genetic background such as some types of cancer are caused by damage in the p53 signaling pathway. The damage changes the system dynamics providing cancer cells with resistance to therapy such as radiation therapy. The change can be observed as the difference in bifurcation diagrams and equilibria type and location between normal and damaged cells, and summarized as the changes of the mathematical model parameters and following changes of the eigenvalues of Jacobian mat...
Source: Journal of Theoretical Biology - August 23, 2016 Category: Biology Source Type: research

siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells
Publication date: May 2018 Source:Micron, Volume 108 Author(s): Janine Berkholz, Regina Eberle, Klaus Boller, Barbara Munz skNAC (skeletal and heart muscle-specific variant of nascent polypeptide-associated complex) and Smyd1 (SET and MYND domain-containing 1) form a protein dimer which is specific for striated muscle cells. Its function is largely unknown. On the one hand, skNAC-Smyd1 appears to control transcriptional processes in the nucleus, on the other hand, specifically at later stages of myogenic differentiation, both proteins translocate to the sarcoplasm and at least Smyd1 specifically associates with sarcomeric...
Source: Micron - March 1, 2018 Category: Biology Source Type: research

Intraluminal delivery of thrombospondin-2 small interfering RNA inhibits the vascular response to injury in a rat carotid balloon angioplasty model Research
In an effort to inhibit the response to vascular injury that leads to intimal hyperplasia, this study investigated the in vivo efficacy of intraluminal delivery of thrombospondin-2 (TSP-2) small interfering RNA (siRNA). Common carotid artery (CCA) balloon angioplasty injury was performed in rats. Immediately after denudation, CCA was transfected intraluminally (15 min) with one of the following: polyethylenimine (PEI)+TSP-2 siRNA, saline, PEI only, or PEI+control siRNA. CCA was analyzed at 24 h or 21 d by using quantitative real-time PCR and immunohistochemistry. TSP-2 gene and protein expression were significantly up-regu...
Source: FASEB Journal - January 2, 2017 Category: Biology Authors: Bodewes, T. C. F., Johnson, J. M., Auster, M., Huynh, C., Muralidharan, S., Contreras, M., LoGerfo, F. W., Pradhan-Nabzdyk, L. Tags: Research Source Type: research

Depletion of CPEB1 protects against oxidized LDL-induced endothelial apoptosis and inflammation though SIRT1/LOX-1 signalling pathway
Publication date: Available online 12 September 2019Source: Life SciencesAuthor(s): Kaicheng Xu, Xiwen liu, Guanghao Ren, Dexin Yin, Suli Guo, Yue ZhaoAbstractAtherosclerosis (AS) is a chronic inflammatory disease that results from Oxidized low-density lipoprotein (Ox-LDL) induced endothelial dysfunction. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is closely related to the development of epithelial cells, but the role of CPEB1 in AS remains unknown. The RNA and protein levels of CPEB1 expression are increased by Ox-LDL exposure, which is abrogated by c-Jun amino-terminal kinase (JNK) inhibitor SP600125. ...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera LeConte, does not involve transitive pathways
This article is protected by copyright. All rights reserved
Source: Insect Science - July 31, 2016 Category: Biology Authors: Huarong Li, Andrew J. Bowling, Premchand Gandra, Murugesan Rangasamy, Heather E. Pence, Robert McEwan, Chitvan Khajuria, Blair Siegfried, Kenneth E. Narva Tags: ORIGINAL ARTICLE Source Type: research

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera, does not involve transitive pathways
This study visualized the spread of the RNAi‐mediated knockdown of Dv v‐ATPase C mRNA throughout the WCR gut and other tissues using high‐sensitivity branched DNA in situ hybridization. Furthermore, we did not detect either secondary siRNA production or transitive RNAi in WCR through siRNA sequence profile analysis. Nucleotide mismatched sequences introduced into either the sense or antisense strand of v‐ATPase C dsRNAs were maintained in siRNAs derived from WCR fed with the mismatched dsRNAs in a strand specific manner. The distribution of all siRNAs was restricted to within the original target sequence regions, w...
Source: Insect Science - November 16, 2016 Category: Biology Authors: Huarong Li, Andrew J. Bowling, Premchand Gandra, Murugesan Rangasamy, Heather E. Pence, Robert E. McEwan, Chitvan Khajuria, Blair D. Siegfried, Kenneth E. Narva Tags: ORIGINAL ARTICLE Source Type: research

Amphiphilic small peptides for delivery of plasmid DNAs and siRNAs
This article is protected by copyright. All rights reserved. As novel nucleic acid carriers, linear and branched amphiphilic peptides were prepared. Each peptide was compared in terms of SNALP size, stability, and delivery activities for plasmid DNA (pDNA) and siRNA. Among the five peptides, a linear peptide H‐RCL was an effective delivery carrier for both siRNA and pDNA. Introduction of a benzoyl group to H‐RCL significantly enhanced siRNA delivery, but greatly reduced pDNA delivery. These demonstrated that a small structural modification of the peptides can modulate their delivery properties.
Source: Chemical Biology and Drug Design - October 20, 2017 Category: Biology Authors: Eun ‐Kyoung Bang, Hanna Cho, Sean S‐H Jeon, Na Ly Tran, Dong‐Kwon Lim, Wooyoung Hur, Taebo Sim Tags: Research Article Source Type: research

Endo-PDI is required for TNFα-induced angiogenesis.
Conclusion: Our study establishes Endo-PDI as a novel, important mediator of AP-1 driven gene expression and endothelial angiogenic function. PMID: 24103565 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - October 5, 2013 Category: Biology Authors: de Lucca Camargo L, Babelova A, Mieth A, Weigert A, Mooz J, Rajalingam K, Heide H, Wittig I, Rossetti Lopes L, Brandes RP Tags: Free Radic Biol Med Source Type: research