Morphological and optical features of the apposition compound eye of Monochamus alternatus Hope (Coleoptera: Cerambycidae)
Publication date: Available online 10 October 2019Source: MicronAuthor(s): Chao Wen, Tao Ma, Yangxiao Deng, Chuanhe Liu, Shiping Liang, Junbao Wen, Cai Wang, Xiujun WenAbstractThe Japanese pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae) is currently the most destructive forest pest as it transmits the pine wilt nematode Bursaphelenchus xylophilus. Morphological, optical features and dark/light adaptational changes of the compound eyes of M. alternatus adults were examined by light, scanning and transmission electron microscopy. The eye of M. alternatus is apposition type and contains 489-712 ommat...
Source: Micron - October 11, 2019 Category: Biology Source Type: research

Automatic Identification of Cashmere and Wool Fibers Based on the Morphological Features Analysis
Publication date: Available online 9 October 2019Source: MicronAuthor(s): Wenyu Xing, Yiwen Liu, Na Deng, Binjie Xin, Wenzhen Wang, Yang ChenAbstractIdentification of wool and cashmere extremely similar fibers is always an important topic in the textile industry. In order to solve this problem much better, a novel fiber recognition method based on the extraction and analysis of the morphological features was proposed in this paper. Firstly, the original fiber images were captured by the self-developed system including the optical microscope and digital camera. The influence of the acquisition process may lead to the low co...
Source: Micron - October 10, 2019 Category: Biology Source Type: research

Comparative study on the effect of Y content on grain refinement in the simulated coarse-grained heat-affected zone of X70 pipeline steels
This study aims to investigate the influence of Y content on grain refinement in the simulated coarse-grained heat-affected zone (CGHAZ) of X70 pipeline steels. The results explored that two different grain refinement mechanisms were presented in 0.034 wt.%-Y and 0.075 wt.%-Y steels, respectively. The Y2O2S inclusions and YP precipitates are formed in 0.034 wt.%-Y steel instead of the YP inclusions and more YP precipitates in 0.075 wt.%-Y steel. The grain refinement mechanism in the simulated CGHAZ of 0.034 wt.%-Y steel mainly depends on the formation of acicular ferrite induced by Y2O2S ...
Source: Micron - October 4, 2019 Category: Biology Source Type: research

Phase masks for electron microscopy fabricated by thermal scanning probe lithography
Publication date: December 2019Source: Micron, Volume 127Author(s): Simon Hettler, Lucas Radtke, Lukas Grünewald, Yuliya Lisunova, Oliver Peric, Juergen Brugger, Simon BonanniAbstractNano-structured phase masks offer intriguing possibilities in electron-beam shaping. The fabrication of such phase masks is typically achieved by focused (Ga+-)ion beam milling of thin membranes. To overcome the problem of Ga implantation in the phase mask, we explore the fabrication of silicon-nitride phase masks using thermal scanning probe lithography combined with wet and dry etching. The functionality of the phase masks is demonstrat...
Source: Micron - October 4, 2019 Category: Biology Source Type: research

Nonlinear indentation of single human erythrocytes under application of a localized mechanical force
Publication date: Available online 30 September 2019Source: MicronAuthor(s): Elisabetta Tognoni, Paolo Orsini, Mario PellegrinoAbstractDespite the accepted notion that erythrocytes are uniquely deformable cells, the apparent Young’s modulus values reported in the literature do not differ so much from those of other cells. We devised to measure the local deformability of living immobilized human erythrocytes at a low force, in contact-free mode, using an application of Scanning Ion Conductance Microscopy (SICM) previously developed in our laboratory. Reversible indentations were induced by forces of up to few hundreds...
Source: Micron - October 1, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: November 2019Source: Micron, Volume 126Author(s): (Source: Micron)
Source: Micron - October 1, 2019 Category: Biology Source Type: research

Bacteria accumulate copper ions and inhibit oxide formation on copper surface during antibacterial efficiency test
Publication date: Available online 24 September 2019Source: MicronAuthor(s): Jiaqi Luo, Christina Hein, Jaafar Ghanbaja, Jean-François Pierson, Frank MücklichAbstractCopper surface after antibacterial test against E. coli was examined in the aspect of corrosion. Results from scanning electron microscope (SEM), grazing incidence X-ray diffractometer (GIXRD) and Raman spectroscopy together confirmed less oxidation on copper surface with the presence of E. coli. The inhibition of the cuprous oxide (Cu2O) layer instead ensured the continuous exposure of copper surface, letting localised corrosion attacks observable...
Source: Micron - September 26, 2019 Category: Biology Source Type: research

Sperm morphology of Elasmus polistis Burks, 1971 (Hymenoptera: Chalcidoidea: Eulophidae)
Publication date: Available online 22 September 2019Source: MicronAuthor(s): Pedro Nere, Glenda Dias, Helen P. Santos, André De Souza, José Lino-NetoAbstractThe sperm morphology of the parasitoid Elasmus polistis (Eulophidae) has been investigated with light and transmission electron microscopy. The sperm were filiform and spiraled, with 165.6 (± 4.6) μm in length, and showed a distinctive head, formed by a one-layered small acrosome and a nucleus, and a flagellar region. An extracellular sheath from which many long filaments radiated out covered the acrosome and part of the nucleus. The spiral nucl...
Source: Micron - September 24, 2019 Category: Biology Source Type: research

Influence of combinatory effects of STEM setups on the sensitivity of differential phase contrast imaging
Publication date: Available online 20 September 2019Source: MicronAuthor(s): Gwangyeob Lee, Juyoung Jeong, Felix Schwarzhuber, Josef Zweck, Jeehoon Kim, Hyang Kim Do, Hye Jung ChangAbstractDifferential phase-contrast (DPC) imaging in the scanning transmission electron microscopy (STEM) mode has been suggested as a new method to visualize the nanoscale electromagnetic features of materials. However, the quality of the DPC image is very sensitive to the electron-beam alignment, microscope setup, and specimen conditions. Unlike normal STEM imaging, the microscope setup variables in the DPC mode are not independent; rather, th...
Source: Micron - September 21, 2019 Category: Biology Source Type: research

Exploring the Accurate size measurement method for two typical nanostructures basing on SEM imaging
Publication date: Available online 20 September 2019Source: MicronAuthor(s): Peng ZhangAbstractThe accurate size measurement has been necessary in modern nanotechnology /semiconductor industry. Based on a sophisticated Monte Carlo scanning electron microscope simulation tool, gold nanorods and the silicon trapezoidal line, two commonly used nanostructures, were studied for aspect ratio and linewidth measurements, respectively. Accurate measurement for aspect ratio is significant for the optical property of gold nanorods. This work adopted an image-based method with a Monte Carlo simulation to obtain an accurate value of th...
Source: Micron - September 21, 2019 Category: Biology Source Type: research

First simultaneous detection of helium and tritium inside bubbles in beryllium
Publication date: Available online 20 September 2019Source: MicronAuthor(s): M. Klimenkov, P. Vladimirov, J. Hoffmann, N. Zimber, A. Möslang, V. KuksenkoAbstractElectron energy loss spectroscopy (EELS) was applied to detect and analyze quantitatively helium (He) and tritium (3H) enclosed inside bubbles in irradiated beryllium. Both gases were formed in beryllium under neutron irradiation as a consequence of neutron-induced transmutation reactions. They were detected for the first time as pronounced peaks at 13.0 eV for 3H and 22.4 eV for He in EELS spectra collected from flat hexagonal bubbles. An adhesi...
Source: Micron - September 21, 2019 Category: Biology Source Type: research

Specific features of the miniature ionisation BSE multi-detector unit for 3D imaging in environmental conditions
Publication date: Available online 9 September 2019Source: MicronAuthor(s): Witold SlówkoAbstractThe author has been striving to develop SEM equipment that could extend the capabilities of standard instruments to allow advanced environmental techniques, including 3D imaging. Now its improved version is presented in a form of the miniature ionisation multi-detector unit that can be accommodated inside a bore of the objective lens and does not limit the minimal Working Distance (WD). The miniature detector unit seems well suited to VP/ESEM of any type to be used instead a standard Pressure Limiting Aperture and even t...
Source: Micron - September 10, 2019 Category: Biology Source Type: research

Analytical analysis and simulation on fringe field effect of deflector plates applied in ultrafast electron microscopy
Publication date: Available online 9 September 2019Source: MicronAuthor(s): Lixin Zhang, Quanlin DongAbstractDeflector plates are one of the critical components in ultrafast electron microscope (UEM), which generates the short electron pulse. While the electron pulse can be stretched due to the velocity dispersion induced by the fringe field of the deflector plates, understanding the effect of the fringe field on the electron velocity and how to avoid the adverse effect of it are needed in the design of the deflector plates. In this paper, firstly, an analytical model of the fringe field of deflector plates with double fie...
Source: Micron - September 10, 2019 Category: Biology Source Type: research

Silver nanoparticles induce cell death of colon cancer cells through impairing cytoskeleton and membrane nanostructure
In this study, we used a silver nanoparticle (AgNP)-based methodology to treat colon cancer cells, and single cell approaches to examine how AgNPs exerted inhibiting effects on cells. We found that AgNPs could apparently destroy cytoskeleton and topography structures, alter cell membrane nanostructures, and thus increase membrane roughness, and depress cell membrane adhesion properties and cell stiffness. We also found that AgNPs caused mitochondrial dysfunctions including hyperpolarization of membrane potential and reactive oxygen species (ROS) accumulation. Notably, AgNPs altered all phenotypes or functions of cells in a...
Source: Micron - September 8, 2019 Category: Biology Source Type: research

3D observation of chromosome scaffold structure using a 360° electron tomography sample holder
Publication date: Available online 8 September 2019Source: MicronAuthor(s): Rinyaporn Phengchat, Misa Hayashida, Nobuko Ohmido, Darren Homeniuk, Kiichi FukuiAbstractThe chromosome scaffold is considered to be a key structure of the mitotic chromosome. It plays a vital role in chromosome condensation, shaping the X-shaped structure of the mitotic chromosome, and also provides flexibility for chromosome movement during cell division. However, it remains to be elucidated how the chromosome scaffold organizes the mitotic chromosome and how it supports shaping the structure of the chromosome during metaphase. Here we present a ...
Source: Micron - September 8, 2019 Category: Biology Source Type: research

Structure, Morphology and Composition of Fur on Different Parts of Reindeer (Rangifer Tarandus) Foot
Publication date: Available online 5 September 2019Source: MicronAuthor(s): Rui Zhang, Guoyu Li, Ruiduo Pan, Qiang Wang, Jianqiao LiAbstractIn the long-distance migration of reindeer in winter, furs of reindeer foot, as the part in direct contacting with the external environment, can play the role of protection and heat preservation. With furs on different parts of the right posterior foot (fibular side, tibial side and planta pedis) as research objects, the microstructure of reindeer foot furs was observed with a scanning electron microscope. The image displayed that the reindeer foot furs was divided into 3 layers, namel...
Source: Micron - September 6, 2019 Category: Biology Source Type: research

Pinopsin and photoreception in the pineal organ of the domestic turkey during post-hatching development
This study was carried out to demonstrate the following: 1) the structures showing immunoreactivity to pinopsin in the turkey pineal organ, 2) the changes of these structures during development, 3) the pinopsin localization in pinealocytes in monolayer cultures, and 4) the role of direct photoreception in the regulation of melatonin secretion in pineal organs in adult turkeys. Pinopsin immunoreactivity was localized in the apical extensions of columnar cells limiting the follicular lumen, in fiber-like structures located between columnar cells in the inner part of follicle wall, in string-shapes or small spherical structur...
Source: Micron - September 4, 2019 Category: Biology Source Type: research

Observation of intracellular protein localization area in a single neuron using gold nanoparticles with a scanning electron microscope
Publication date: Available online 31 August 2019Source: MicronAuthor(s): Toichiro Goto, Nahoko Kasai, Roxana Filip, Koji Sumitomo, Hiroshi NakashimaAbstractThe localization areas of intracellular proteins in rat cortical neurons were visualized using a scanning electron microscope (SEM) coupled with a focused ion beam (FIB) system. To obtain a clear contrast in the SEM images, gold nanoparticles (GNPs) were bound to specific intracellular proteins by antigen-antibody reactions. By obtaining a cross section of the desired location of the neurons by FIB milling under the SEM imaging condition, it was possible to observe the...
Source: Micron - September 1, 2019 Category: Biology Source Type: research

Seasonal exploration of ultrastructure and Na+/K+-ATPase, Na+/K+/2Cl– cotransporter of mitochondria-rich cells in the small intestine of turtles
Publication date: Available online 29 August 2019Source: MicronAuthor(s): Waseem Ali Vistro, Imran Tarique, Abdul Haseeb, Ping Yang, Yufei Huang, Hong Chen, Xuebing Bai, Surfaraz Ali Fazlani, Qiusheng ChenAbstractDespite the exploration of mitochondria-rich cells (MRCs) in different animal classes, very limited information has been documented about MRCs in reptiles. The present study was designed to investigate the effect of seasonal variation on the cell ultrastructure and ion transport protein activity of MRCs during hibernation and non-hibernation of Chinese soft-shelled turtle’s intestine. Transmission electron m...
Source: Micron - August 31, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: October 2019Source: Micron, Volume 125Author(s): (Source: Micron)
Source: Micron - August 29, 2019 Category: Biology Source Type: research

Short-range ordering induced serrated flow in a carbon contained FeCoCrNiMn high entropy alloy
Publication date: Available online 23 August 2019Source: MicronAuthor(s): Lin Guo, Ji Gu, Xing Gong, Kai Li, Song Ni, Yong Liu, Min SongAbstractPhase stability of high entropy alloys depends significantly on the compositional constituents. Here we report the joint effects of interstitial carbon and compositional inhomogeneity on the mechanical response, particularly the serrated flow, of a carbon doped FeCoCrMnNi high entropy alloy. Detailed microstructural characterizations show that compositional inhomogeneity, including heterogeneous short-range ordering structure, is formed in carbon doped FeCoCrNiMn HEA. The serrated ...
Source: Micron - August 25, 2019 Category: Biology Source Type: research

A Circuit Model for SECCM and Topographic Imaging Method In AC Mode
Publication date: Available online 23 August 2019Source: MicronAuthor(s): Jian Zhuang, Xiaobo Liao, Yalou Deng, Lei Cheng, Ali Akmal Zia, Yong Cai, Maolin ZhouABSTRACTSingle-barrel scanning electrochemical cell microscopy (SECCM) can be used to perform electrochemical activity analysis and sample surface imaging simultaneously. Compared to SECM & SICM in imaging, the most significant advantage of SECCM is that it does not need to immerse sample in solution, which avoids the electrochemical reaction between electrolyte and sample surface. In traditional direct current (DC) topographic imaging method of SECCM, when the m...
Source: Micron - August 25, 2019 Category: Biology Source Type: research

Wave-packet numerical investigation of thermal diffuse scattering: A time-dependent quantum approach to electron diffraction simulations
Publication date: Available online 23 August 2019Source: MicronAuthor(s): Samantha Rudinsky, Angel S. Sanz, Raynald GauvinAbstractThe effects of thermal diffuse scattering on diffraction of highly-accelerated electrons by crystal lattices are investigated with a method that combines the frozen phonon approximation with an exact numerical solution of the time-dependent Schrödinger equation. The phonon configuration for each single-electron diffraction process is determined by means of Einstein's model. It is shown that this procedure provides the possibility of describing and explaining, in a natural way, after averagi...
Source: Micron - August 25, 2019 Category: Biology Source Type: research

An Engineering Perspective on the Microstructure and Compression Properties of the Seagull Larus argentatus Feather Rachis
In this study, the microstructure properties of L. argentatus feather rachis were analysed by scanning electron microscopy (SEM). These analysis methods enabled the configuration, structure and compression properties of the rachis to be investigated. The results indicated that the rachis was composed of the outer cortex and the inner medulla. The cortex had a continuous layered nano-fibre composite structure, which bears, transmits, absorbs and disperses the compression force. The medulla had bubble-like cells with a porous-fibre structure, which rapidly absorbs, transmits and consumes compression force and is a suitably l...
Source: Micron - August 20, 2019 Category: Biology Source Type: research

Automated Stitching of Microscope Images of Fluorescence in Cells with Minimal Overlap
In conclusion, quantitative evaluation of the performance of the method proved its improved accuracy compared to other current state-of-art techniques, and it showed robust performance even with noise and a narrow overlapping region between adjacent fields. (Source: Micron)
Source: Micron - August 17, 2019 Category: Biology Source Type: research

Morphological study of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) eggs by X-ray computed microtomography
Publication date: Available online 16 August 2019Source: MicronAuthor(s): Lilian Mirian Oliveira de Morais, Eduardo Inocente Jussiani, João Antonio Cyrino Zequi, Paulo José dos Reis, Avacir Casanova AndrelloAbstractAedes aegypti and Aedes albopictus are vectors of several arboviruses responsible for causing dengue, chikungunya, Zika, and yellow fever viruses and microcephaly, resulting in a public health problem in several countries worldwide. Even in this scenario, studies on the morphology of mosquito eggs are still lacking. In this paper, X-ray microtomography was used to study mosquito egg morphology. Sev...
Source: Micron - August 17, 2019 Category: Biology Source Type: research

Correlative FLIM-Confocal-Raman mapping applied to plant lignin composition and autofluorescence
We present a method for correlative FLIM-confocal-Raman imaging and its application to lignin composition studies in the woody stems of the plant model Arabidopsis thaliana. Lignin is autofluorescent and exhibits characteristic fluorescence lifetimes attributed to its composition. Its composition can be further resolved by Raman microscopy to multiple peaks that represent different components. A lignin biosynthetic mutant is found to have a marked difference in fluorescence lifetime and corresponds to a change in composition as demonstrated by the Raman output. (Source: Micron)
Source: Micron - August 12, 2019 Category: Biology Source Type: research

Effect of propionic acid on the morphology of the amygdala in adolescent male rats and their behavior
Publication date: Available online 6 August 2019Source: MicronAuthor(s): Giorgi Lobzhanidze, Mzia Zhvania (Source: Micron)
Source: Micron - August 7, 2019 Category: Biology Source Type: research

Variability and composition of calcium oxalate crystals in embryos-seedlings-adult plants of the globose cacti Mammillaria uncinata
Publication date: Available online 2 August 2019Source: MicronAuthor(s): Brenda Micaela López-Macías, Sandra Elena Morales-Martínez, Sofía Loza-Cornejo, Claudio Frausto-Reyes, Teresa Terrazas, Rita Judith Patakfalvi, Martín Ortiz-Morales, María de la Luz Miranda BeltránAbstractThe morphology and elemental composition of crystals in embryos, seedlings and adult plants of the globose cacti Mammillaria uncinata were studied. Samples of mature fruits and adult plants were collected. To obtain embryos and seedlings, seeds from mature fruits were germinated under laboratory condit...
Source: Micron - August 3, 2019 Category: Biology Source Type: research

Human macrophages and osteoclasts resorb β-tricalcium phosphate in vitro but not mouse macrophages
Publication date: Available online 30 July 2019Source: MicronAuthor(s): Baptiste Arbez, Florence Manero, Guillaume Mabilleau, Hélène Libouban, Daniel ChappardAbstractβ-TCP is a resorbable bony biomaterial but its biodegradation mechanisms in vivo remains unclear. Osteoclast can resorb β-TCP but a role for macrophages has also been suggested by in vivo studies. However no in vitro study has clearly evidenced the action of macrophages in the resorption process. We prepared flat β-TCP tablets with a smooth surface to investigate the in vitro capability of murine (RAW 264.7) and human macrophage ce...
Source: Micron - July 31, 2019 Category: Biology Source Type: research

A SEM Study of Unexplored Wax Microtubes Produced by Caterpillar Living on Common Banana Leaves
Publication date: Available online 29 July 2019Source: MicronAuthor(s): Salprima Yudha S., Aswin Falahudin, Deni Agus Triawan, Morina AdfaAbstractThe primary concern of this research is to perform a microscopic analysis of the natural wax covering banana skipper caterpillar. The scanning electron microscopic (SEM) analysis shows that the wax exhibits an unprecedented microtubular form, consisting of carbon and oxygen as main elements as revealed by energy dispersive X-ray (EDX) analysis. The Fourier Transform Infra-Red (FTIR) spectroscopic analysis, which reveals the presence of peaks at 2914, 2890, 1469, and 722 cm&thinsp...
Source: Micron - July 30, 2019 Category: Biology Source Type: research

Preface – Electron microscopy of beam-sensitive materials
Publication date: October 2019Source: Micron, Volume 125Author(s): Thomas Slater (Source: Micron)
Source: Micron - July 30, 2019 Category: Biology Source Type: research

Towards more reliable AFM force-curve evaluation: a method for spring constant selection, adaptive lever sensitivity calibration and fitting boundary identification
Publication date: Available online 26 July 2019Source: MicronAuthor(s): Judit Kámán, Róbert Huszánk, Attila BonyárAbstractThis work discusses key issues regarding the atomic force microscopy (AFM) force-curve evaluation practice, which can affect the determined Young’s modulus of the investigated sample. These issues are 1) the proper calibration of lever sensitivity and the effect of its variation between the measurements; 2) the selection of proper cantilever spring constant for the investigated sample; and 3) the selection of the fitting boundaries for the contact mechanics model...
Source: Micron - July 27, 2019 Category: Biology Source Type: research

Editorial Board
Publication date: September 2019Source: Micron, Volume 124Author(s): (Source: Micron)
Source: Micron - July 27, 2019 Category: Biology Source Type: research

Comparative sperm morphology of Proceratophrys and Odontophrynus (Anura, Odontophrynidae)
Publication date: Available online 25 July 2019Source: MicronAuthor(s): Julio Sérgio Santos, William Pinheiro da Costa, Karin Regina Seger, Shirlei Maria Recco-PimentelAbstractThe ultrastructure of the spermatozoa of seven species of Proceratophrys and one of Odontophrynus was analyzed in the present study. The sperm of Odontophrynus juquinha is similar to that of Odontophrynus cultripes, described previously, and to those of Proceratophrys branti, Proceratophrys bigibbosa, Proceratophrys affinis concavitympanum, Proceratophrys huntingtoni, Proceratophrys conformis moratoi, P. moratoi, and Proceratophrys palustris, ...
Source: Micron - July 27, 2019 Category: Biology Source Type: research

Mapping the strain distribution within embedded nanoparticles via geometrical phase analysis
Publication date: Available online 23 July 2019Source: MicronAuthor(s): Yan Wang, Wei ZhangAbstractStrain variation within a nanoparticle plays a crucial role in tuning its properties. Geometrical phase analysis (GPA) is typically a powerful tool to investigate the strain in high-resolution transmission electron microscopy (HRTEM) images. It is known that the traditional GPA method measuring the displacement of lattice fringes directly in an HRTEM image is inapplicable to strain measurements on nanoparticles embedded in a matrix, where lattice fringes of nanoparticles are invisible, instead Moiré fringes are present...
Source: Micron - July 24, 2019 Category: Biology Source Type: research

Effect of density and Z-contrast on the visibility of noble gas precipitates and voids with insights from Monte-Carlo simulations
Publication date: Available online 11 July 2019Source: MicronAuthor(s): Anamul H. Mir, Jonathan. A. Hinks, Stephen. E. DonnellyAbstractIn this work, a detailed analysis of He, Ne, Ar, Kr and Xe precipitates in a complex borosilicate glass using transmission electron microscopy (TEM) with in-situ ion implantation is presented. With in-situ monitoring, the real-time dynamics of precipitate and void evolution under ion implantation was followed. Using appropriate equations of state and, Monte-Carlo simulations to supplement the TEM images, we then discuss in detail the possibility and ways of differentiating the precipitates ...
Source: Micron - July 12, 2019 Category: Biology Source Type: research

Heat transfer in the Tank-Inflorescence of Nidularium innocentii (Bromeliaceae): Experimental and Finite Element Analysis based on X-ray microtomography
This study investigates the role of water in the flowers and inflorescences of Nidularium innocentii, and describes water as a protection mechanism. Individuals were divided into groups with and without water provision in inflorescences. Both groups were maintained with water in soil and leaves under the same environmental conditions. During anthesis, individuals were collected, and inflorescences were measured. Another specimen was prepared and scanned using X-ray microtomography (µCT), generating a high-resolution 3D model that was converted into a discretized geometry. Heat transfer finite element analysis (FEA) o...
Source: Micron - July 12, 2019 Category: Biology Source Type: research

Coherent strain of Guinier–Preston II zone in an Al–Zn–Mg–Cu alloy
Publication date: Available online 2 July 2019Source: MicronAuthor(s): Xiaohu Hou, Jijun Li, Fei Liu, Liangming Yan, Pucun BaiAbstractThe Guinier-Preston (GP) II zone in an Al–Zn–Mg–Cu alloy was investigated by using a high-resolution electron microscopy (HREM). We used Geometric Phase Analysis (GPA) technique to measure the experimental HREM image and the simulated HREM image to obtain the strain distribution maps. The results show converging strain fields in the GP II zone, and the maximum compressive strain can reach ―7.2% and ―6.7%, respectively. The distribution of the experimental strain field w...
Source: Micron - July 3, 2019 Category: Biology Source Type: research

Effect of extract from ginseng rust rot on the inhibition of human hepatocellular carcinoma cells in vitro
In this study, the HCC cells were treated with different parts of mountain cultivated ginseng rust rot and compared with human normal liver cells. The morphology, survival rate and β-actin expression of the cells were changed by introducing the ginseng epidermis during the incubation process. Notably, the results reveal that the ginseng epidermis can induce apoptosis by altering the morphologies of cells, indicating the practical implication for the HCC treatment and drug development. (Source: Micron)
Source: Micron - July 3, 2019 Category: Biology Source Type: research

Microscopic imaging quality improvement through L0 gradient constraint model based on multi-fields of view analysis
Publication date: Available online 29 June 2019Source: MicronAuthor(s): Jufeng Zhao, Chao Wu, Weiping Hua, Guangmang Cui, Keqi Zhang, Yu Zhang, Yue ZangAbstractThe degradation of optical microscopic imaging is space-variant, and how to fast restore optical degraded image remains a special problem. Based on point spread function (PSF) estimation under each field of view (FOV), a L0 gradient-constrained image restoration method is proposed to solve optical degradation in microscopic imaging. Firstly, the whole scene is segmented into several different regions according to different FOV. The PSFs for each region are estimated...
Source: Micron - June 30, 2019 Category: Biology Source Type: research

Improved Vinegar & Wellington Calibration for Estimation of Fluid Saturation and Porosity from CT Images for a Core Flooding Test under Geologic Carbon Storage Conditions
Publication date: Available online 29 June 2019Source: MicronAuthor(s): Xiuxiu Miao, Yan Wang, Liwei Zhang, Ning Wei, Xiaochun LiAbstractX-ray computed tomography (CT) of fluid flow in formation rocks is an important characterization technique in geologic carbon sequestration research to provide insight into the migration and capillary trapping of CO2 under reservoir conditions. An improved calibration method adapted from traditional Vinegar & Wellington calibration is proposed to map the 3D pore and fluid distributions from the CT images of CO2/brine displacement flooding. Similar to Vinegar & Wellington calibrati...
Source: Micron - June 30, 2019 Category: Biology Source Type: research

Nitrogen deprivation elicits dimorphism, capsule biosynthesis and autophagy in Papiliotrema laurentii strain RY1
Publication date: Available online 26 June 2019Source: MicronAuthor(s): Soumyadev Sarkar, Avishek Mukherjee, Subhadeep Das, Bidisha Ghosh, Shouvik Chaudhuri, Debanjana Bhattacharya, Arpita Sarbajna, Ratan GachhuiAbstractStress response due to the lack of essential nutrient(s) for an organism has been a focal point of several scientific investigations. The present study investigates the cellular adaptations behind the ability of Papiliotrema laurentii strain RY1 to perpetuate without added nitrogen and propagate robustly in growth- limiting amount of nitrogen. We executed phenotypic (using scanning electron microscopy, diff...
Source: Micron - June 27, 2019 Category: Biology Source Type: research

Guy Cox, Fundamentals of Fluorescence Imaging, 2019, Jenny Stanford Publishing; Singapore.
Publication date: September 2019Source: Micron, Volume 124Author(s): Marc A.M.J. van Zandvoort (Source: Micron)
Source: Micron - June 27, 2019 Category: Biology Source Type: research

Identification of collagen fibrils in cross sections of bone by electron energy loss spectroscopy (EELS)
Publication date: Available online 20 June 2019Source: MicronAuthor(s): Bryan E.J. Lee, Lucy Luo, Kathryn Grandfield, Carmen M. Andrei, Henry P. SchwarczAbstractTransmission electron microscopic (TEM) images of ion-milled bovid cortical bone cut approximately normal to the axes of fibrils show that mineral occurs in the form of plates surrounding and laying between circular or elliptical features about 50 nm in diameter. The classification of these features as either pores or collagen fibrils is highly debated. Electron energy loss spectroscopy (EELS) mapping of these features in ion milled sections shows that they ...
Source: Micron - June 20, 2019 Category: Biology Source Type: research

Book Review “Fundamentals of Fluorescence Imaging”.
Publication date: Available online 19 June 2019Source: MicronAuthor(s): Marc A.M.J. van Zandvoort (Source: Micron)
Source: Micron - June 19, 2019 Category: Biology Source Type: research

Application of a layered model for determination of the elasticity of biological systems
Publication date: Available online 15 June 2019Source: MicronAuthor(s): M. Rusaczonek, B. Zapotoczny, M. Szymonski, J. KoniorAbstractElasticity of biological systems is considered to be an important property that might be related to functional or pathological changes. Therefore, careful study and detailed understanding of cell and tissue elasticity is crucial for correct description of their functioning. Atomic force microscopy (AFM) is a powerful technique, which allows for determination of the physical properties, such as elasticity, of soft-matter systems in nano-scale. An important step in AFM elasticity studies is a p...
Source: Micron - June 16, 2019 Category: Biology Source Type: research

Volumetric estimate of bordered pits in Pinus sylvestris based on X-ray tomography and light microscopy imaging
Publication date: Available online 13 June 2019Source: MicronAuthor(s): Tim Koddenberg, Maximilian Wentzel, Holger MilitzAbstractBordered pits are a major determinant for the hydraulic function of wood tissues. Unlike microscopic imaging (e.g. light and electron microscopy) that is constrained to two-dimensional (2D) information, X-ray micro-computed tomography (XμCT) contributes to three-dimensional (3D) analysis. This advantage was used to estimate the volume of bordered pits in Pinus sylvestris. The 3D data obtained by XµCT were compared with two mathematical models (ellipsoid model and spherical cap models) us...
Source: Micron - June 15, 2019 Category: Biology Source Type: research

A rapid method of whole cell sample preparation for scanning electron microscopy using neodymium chloride
Publication date: Available online 13 June 2019Source: MicronAuthor(s): Ivan Novikov, Igor ChebotarAbstractThe quality of electron microscopy (EM) visualization of biological objects is constantly improving, primarily with the usage of more complex technologies, such as serial block-face scanning electron microscopy (SEM), focused ion beam scanning electron microscopy, and array tomography. Here we suggest a new rapid method of whole cell sample preparation for scanning EM using neodymium chloride treatment followed by staining with lead acetate. This variant of sample preparation does not require separate fixation, comple...
Source: Micron - June 13, 2019 Category: Biology Source Type: research

Mechanical characterisation of pentagonal gold nanowires in three different test configurations: A comparative study
Publication date: Available online 8 June 2019Source: MicronAuthor(s): Mikk Antsov, Boris Polyakov, Vahur Zadin, Magnus Mets, Sven Oras, Mikk Vahtrus, Rünno Lõhmus, Leonid Dorogin, Sergei VlassovAbstractMechanical characterisation of individual nanostructures is a challenging task and can greatly benefit from the utilisation of several alternative approaches to increase the reliability of results. In the present work, we have measured and compared the elastic modulus of five-fold twinned gold nanowires (NWs) with atomic force microscopy (AFM) indentation in three different test configurations: three-point bendi...
Source: Micron - June 9, 2019 Category: Biology Source Type: research