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Condition: Diabetes

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Total 1499 results found since Jan 2013.

Pterostilbene attenuates high glucose-induced oxidative injury in hippocampal neuronal cells by activating nuclear factor erythroid 2-related factor 2
Publication date: Available online 9 January 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Author(s): Yang Yang, Chongxi Fan, Bodong Wang, Zhiqiang Ma, Dongjin Wang, Bing Gong, Shouyin Di, Shuai Jiang, Yue Li, Tian Li, Zhi Yang, Erping Luo In the present study, neuroblastoma (SH-SY5Y) cells were used to investigate the mechanisms mediating the potential protective effects of pterostilbene (PTE) against mitochondrial metabolic impairment and oxidative stress induced by hyperglycemia for mimicking the diabetic encephalopathy. High glucose medium (100 mM) decreased cellular viability after 24 h...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - January 8, 2017 Category: Molecular Biology Source Type: research

High Glucose-Induced Hypomethylation Promotes Binding of Sp-1 to Myo-Inositol Oxygenase: Implication in the Pathobiology of Diabetic Tubulopathy.
Abstract The catabolic enzyme myo-inositol oxygenase (MIOX) is expressed in proximal tubules and up-regulated in the diabetic state. Previously, we reported its transcriptional and translation regulation by high glucose (HG), osmolytes, and fatty acids. However, its epigenetic regulation is unknown. Bisulfite sequencing revealed that both human and mouse MIOX promoters, enriched with CpG sites, are hypomethylated and unmethylated under HG ambience and hyperglycemic states associated with increased MIOX expression. Eletrophoretic mobility shift assays revealed increased binding of unmethylated oligos with nucleopro...
Source: The American Journal of Pathology - February 12, 2017 Category: Pathology Authors: Sharma I, Dutta RK, Singh NK, Kanwar YS Tags: Am J Pathol Source Type: research

MicroRNA-29 regulates myocardial microvascular endothelial cells proliferation and migration in association with IGF1 in type 2 diabetes.
CONCLUSION: Our study suggests that miR-29 downregulation, through its inverse regulation on downstream target of IGF1 gene, is a pro-angiogenic factor in MMEVC in type 2 diabetic rats. PMID: 28315330 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - March 14, 2017 Category: Biochemistry Authors: Li Z, Yue Q, Peng H Tags: Biochem Biophys Res Commun Source Type: research

Transcription factor SP1 mediates hyperglycemia-induced upregulation of roundabout4 in retinal microvascular endothelial cells.
In this study, we explored the mechanism of transcriptional regulation of Robo4 in retinal endothelial cells, and investigated the effects of this regulation on cellular functions under hyperglycemic conditions. Human retinal endothelial cells (HREC) exposed to hyperglycemia were used to detect the expression levels of specificity protein 1 (SP1) and Robo4 by RT-qPCR and western blotting. Small interfering RNA (SiRNA) transfection technology was used to analyze the regulatory relationship between SP1 and Robo4. The effect of transcription factor SP1 on Robo4 promoter activity and the location of SP1 binding sites were inve...
Source: Gene - March 21, 2017 Category: Genetics & Stem Cells Authors: Xie J, Gong Q, Liu X, Liu Z, Tian R, Cheng Y, Su G Tags: Gene Source Type: research

Klotho down-regulates Egr-1 by inhibiting TGF- β1/Smad3 signaling in high glucose treated human mesangial cells.
Klotho down-regulates Egr-1 by inhibiting TGF-β1/Smad3 signaling in high glucose treated human mesangial cells. Biochem Biophys Res Commun. 2017 Apr 11;: Authors: Li Y, Hu F, Xue M, Jia YJ, Zheng ZJ, Wang L, Guan MP, Xue YM Abstract Diabetic kidney disease (DKD) has become the leading cause of end-stage renal disease worldwide and is associated with glomerular mesangial cell (MC) proliferation and excessive extracellular matrix (ECM) production. Klotho can attenuate renal fibrosis in part by inhibiting TGF-β1/Smad3 signaling in DKD. Early growth response factor 1 (Egr-1) has been shown to play a key...
Source: Biochemical and Biophysical Research communications - April 11, 2017 Category: Biochemistry Authors: Li Y, Hu F, Xue M, Jia YJ, Zheng ZJ, Wang L, Guan MP, Xue YM Tags: Biochem Biophys Res Commun Source Type: research

Epigallocatechin Gallate Upregulates NRF2 to Prevent Diabetic Nephropathy via Disabling KEAP1.
Abstract Epigallocatechin gallate (EGCG) is the most abundant and effective green tea catechin and has been reported to attenuate diabetic nephropathy (DN). However, the mechanism by which EGCG ameliorates DN, till now, has remained unclear. EGCG is known as a potent activator of nuclear factor erythroid 2-related factor 2 (NRF2), which plays a key role in cellular defense against diabetes-induced oxidative stress and in the prevention of DN. In the present study, we tested whether NRF2 is required for EGCG protection against DN. Therefore, C57BL/6 wild type (WT) and Nrf2 knockout mice were induced to diabetes by ...
Source: Free Radical Biology and Medicine - April 27, 2017 Category: Biology Authors: Sun W, Liu X, Zhang H, Song Y, Li T, Liu X, Liu Y, Guo L, Wang F, Yang T, Guo W, Wu J, Jin H, Wu H Tags: Free Radic Biol Med Source Type: research

Inhibition of long noncoding RNA IGF2AS promotes angiogenesis in type 2 diabetes.
CONCLUSION: IGF2AS inhibition has angiogenic effect in diabetic GK mMVE cells. The functions of IGF2AS in type 2 diabetes are very likely through the inverse regulation of IGF2, but independent of IGF1. PMID: 28570978 [PubMed - as supplied by publisher]
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - May 29, 2017 Category: Drugs & Pharmacology Authors: Zhao Z, Liu B, Li B, Song C, Diao H, Guo Z, Li Z, Zhang J Tags: Biomed Pharmacother Source Type: research

Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats.
CONCLUSIONS Silencing of TRB3 has a significant ameliorative effect on diabetic nephropathy in rats. PMID: 28600485 [PubMed - in process]
Source: Medical Science Monitor - June 11, 2017 Category: Research Tags: Med Sci Monit Source Type: research

Long noncoding RNA BDNF ‐AS inversely regulated BDNF and modulated high‐glucose induced apoptosis in human retinal pigment epithelial cells
Conclusion: Our results suggest that BDNF‐AS, through inverse regulation of BDNF, might play a critical role in the process of DGI apoptosis in diabetic retinopathy. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - June 28, 2017 Category: Biochemistry Authors: Yanyan Li, Feng Xu, Hanyan Xiao, Feng Han Tags: Article Source Type: research

Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats.
Authors: Guo X, Liu X Abstract Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM). We investigated whether Nogo receptor (NgR) knockdown and ciliary neurotrophic factor (CNTF) treatment, either alone or in combination, ameliorated diabetic retinopathy (DR) in diabetic rat model. STZ‑induced diabetic rats were administrated for a total of 12 weeks with 3 µM siRNA (5 µl) once every 6 weeks and/or 1 µg CNTF weekly. The retinal tissues were excised. We measured cell number in ganglion cell layer (GCL) using H&E staining and cell apoptosis using TUNEL assay. Bax, Bcl‑2, Caspase...
Source: Molecular Medicine Reports - June 29, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

(-)-Epigallocatechin-3-gallate attenuates myocardial injury induced by ischemia/reperfusion in diabetic rats and in H9c2 cells under hyperglycemic conditions.
In this study, we aimed to examine the effects of EGCG on diabetic cardiomyopathy during myocardial ischemia/reperfusion (I/R) injury. EGCG (100 mg/kg/day) was administered at week 6 for 2 weeks to diabetic rats following the induction of type 1 diabetes by streptozotocin (STZ). At the end of week 8, the animals were subjected to myocardial I/R injury. The EGCG-elicited structural and functional effects were analyzed. Additionally, EGCG (20 µM) was administered for 24 h to cultured cardiac H9c2 cells under hyperglycemic conditions (30 mM glucose) prior to hypoxia/reoxygenation (H/R) challenge, and its effec...
Source: International Journal of Molecular Medicine - July 19, 2017 Category: Molecular Biology Authors: Wu Y, Xia ZY, Zhao B, Leng Y, Dou J, Meng QT, Lei SQ, Chen ZZ, Zhu J Tags: Int J Mol Med Source Type: research

Role of HSP60 (HSPD1) in diabetes-induced renal tubular dysfunction: regulation of intracellular protein aggregation, ATP production, and oxidative stress Research
Because underlying mechanisms of diabetic nephropathy/tubulopathy remained poorly understood, we aimed to define a key protein involving in hyperglycemia-induced renal tubular dysfunction. All altered renal proteins identified from previous large-scale proteome studies were subjected to global protein network analysis, which revealed heat shock protein 60 (HSP60, also known as HSPD1) as the central node of protein–protein interactions. Functional validation was performed using small interfering RNA (siRNA) to knock down HSP60 (siHSP60). At 48 h after exposure to high glucose (HG) (25 mM), Madin-Darby canine kidney (M...
Source: FASEB Journal - May 1, 2017 Category: Biology Authors: Aluksanasuwan, S., Sueksakit, K., Fong-ngern, K., Thongboonkerd, V. Tags: Research Source Type: research

Advanced glycation end ‐products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells
This study aimed at investigating the effects of AGE and Porphyromonas gingivalis lipopolysaccharide (PgLPS) on calprotectin expression in the human gingival epithelial cell line OBA‐9. AGE and PgLPS increased the expression of S100A8 and S100A9 mRNAs, and AGE + PgLPS co‐stimulation amplified their expression in OBA‐9 cells. A higher concentration of calprotectin in cell lysates was also induced by stimulation with AGE and/or PgLPS. S100A8 was mainly translocated from the nucleus to the cytoplasm by AGE stimulation, while cytoplasmic localization of S100A9 was not altered following stimulation with AGE and/or PgL...
Source: Journal of Cellular Biochemistry - August 3, 2017 Category: Biochemistry Authors: Yuka Hiroshima, Eijiro Sakamoto, Kaya Yoshida, Kaori Abe, Koji Naruishi, Takenori Yamamoto, Yasuo Shinohara, Jun ‐ichi Kido, Carolyn L. Geczy Tags: Article Source Type: research

Resveratrol alleviates diabetic cardiomyopathy in rats by improving mitochondrial function through PGC-1 α deacetylation.
In this study we examined whether resveratrol alleviated cardiac dysfunction in diabetes by improving mitochondrial function via SIRT1-mediated PGC-1α deacetylation. T2DM was induced in rats by a high-fat diet combined with STZ injection. Diabetic rats were orally administered RSV (50 mg·kg(-1)·d(-1)) for 16 weeks. RSV administration significantly attenuated diabetes-induced cardiac dysfunction and hypertrophy evidenced by increasing ejection fraction (EF%), fraction shortening (FS%), ratio of early diastolic peak velocity (E velocity) and late diastolic peak velocity (A velocity) of the LV inflow (E/A ratio) and reduci...
Source: Acta Pharmacologica Sinica - August 3, 2017 Category: Drugs & Pharmacology Authors: Fang WJ, Wang CJ, He Y, Zhou YL, Peng XD, Liu SK Tags: Acta Pharmacol Sin Source Type: research

Autophagy inhibits high glucose induced cardiac microvascular endothelial cells apoptosis by mTOR signal pathway
AbstractCardiac microvascular endothelial cells (CMECs) dysfunction is an important pathophysiological event in the cardiovascular complications induced by diabetes. However, the underlying mechanism is not fully clarified. Autophagy is involved in programmed cell death. Here we investigated the potential role of autophagy on the CMECs injury induced by high glucose. CMECs were cultured in normal or high glucose medium for 6, 12 and 24  h respectively. The autophagy of CMECs was measured by green fluorescence protein (GFP)-LC3 plasmid transfection. Moreover, the apoptosis of CMEC was determined by flow cytometry. Furtherm...
Source: Apoptosis - August 20, 2017 Category: Molecular Biology Source Type: research