Holistic review of polysaccharides isolated from pumpkin: Preparation methods, structures and bioactivities
Int J Biol Macromol. 2021 Oct 14:S0141-8130(21)02189-9. doi: 10.1016/j.ijbiomac.2021.10.037. Online ahead of print.ABSTRACTPumpkin polysaccharides have arrested researchers' attention in fields of food supplements for healthy product and traditional Chinese medicine due to their multiple bioactivities with non-toxic and highly biocompatible. This review emphatically summarized recent progresses in the primary and spatial structural features, various bioactivities, structure-to-function associations, different preparation techniques, and absorption characteristics across intestinal epithelial and in vivo bio-distribution of...
Source: International Journal of Biological Macromolecules - October 17, 2021 Category: Biochemistry Authors: Fei Li Jing Zhao Yunlu Wei Xu Jiao Quanhong Li Source Type: research

Pumpkin (Cucurbita moschata) HSP20 Gene Family Identification and Expression Under Heat Stress
Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop that has strong resistance to abiotic stress. While heat shock protein 20 (HSP20) has been implicated in vegetable response to heat stress, little is known regarding activity of HSP20 family proteins in C. moschata. Here, we performed a comprehensive genome-wide analysis to identify and characterize the functional dynamics of the Cucurbita moschata HSP20 (CmoHSP20) gene family. A total of 33 HSP20 genes distributed across 13 chromosomes were identified from the pumpkin genome. Our phylogenetic analysis determined that the CmoHSP20 proteins fell into nine ...
Source: Frontiers in Genetics - October 14, 2021 Category: Genetics & Stem Cells Source Type: research

Sensors, Vol. 21, Pages 6657: Yield Estimation and Visualization Solution for Precision Agriculture
We present an end-to-end smart harvesting solution for precision agriculture. Our proposed pipeline begins with yield estimation that is done through the use of object detection and tracking to count fruit within a video. We use and train You Only Look Once model (YOLO) on video clips of apples, oranges and pumpkins. The bounding boxes obtained through objection detection are used as an input to our selected tracking model, DeepSORT. The original version of DeepSORT is unusable with fruit data, as the appearance feature extractor only works with people. We implement ResNet as DeepSORT's new feature extractor, which is...
Source: Sensors - October 7, 2021 Category: Biotechnology Authors: Osman Dennis Elgazzar Tags: Article Source Type: research

Characterization of recombinant pumpkin 2S albumin and mutation studies to unravel potential DNA/RNA binding site
Biochem Biophys Res Commun. 2021 Sep 29;580:28-34. doi: 10.1016/j.bbrc.2021.09.076. Online ahead of print.ABSTRACTThe native pumpkin 2S albumin, a multifunctional protein, possess a variety of potential biotechnologically exploitable properties. The present study reports the characterization of recombinant pumpkin 2S albumin (rP2SA) and unraveling of its potential DNA/RNA binding site. The purification and characterization of the rP2SA established that it retains the characteristic α-helical structure and exhibited comparable DNase, RNase, antifungal and anti-proliferative activities as native protein. In vitro studies re...
Source: Biochemical and Biophysical Research communications - October 5, 2021 Category: Biochemistry Authors: Brajesh Kumar Savita Vikram Dalal Shweta Choudhary Deena Nath Gupta Neeladrisingha Das Shailly Tomar Pravindra Kumar Partha Roy Ashwani Kumar Sharma Source Type: research

Characterization of recombinant pumpkin 2S albumin and mutation studies to unravel potential DNA/RNA binding site
Biochem Biophys Res Commun. 2021 Sep 29;580:28-34. doi: 10.1016/j.bbrc.2021.09.076. Online ahead of print.ABSTRACTThe native pumpkin 2S albumin, a multifunctional protein, possess a variety of potential biotechnologically exploitable properties. The present study reports the characterization of recombinant pumpkin 2S albumin (rP2SA) and unraveling of its potential DNA/RNA binding site. The purification and characterization of the rP2SA established that it retains the characteristic α-helical structure and exhibited comparable DNase, RNase, antifungal and anti-proliferative activities as native protein. In vitro studies re...
Source: Biochemical and Biophysical Research communications - October 5, 2021 Category: Biochemistry Authors: Brajesh Kumar Savita Vikram Dalal Shweta Choudhary Deena Nath Gupta Neeladrisingha Das Shailly Tomar Pravindra Kumar Partha Roy Ashwani Kumar Sharma Source Type: research

The effects of cellulose nanocrystal and cellulose nanofiber on the properties of pumpkin starch-based composite films
Int J Biol Macromol. 2021 Oct 1:S0141-8130(21)02115-2. doi: 10.1016/j.ijbiomac.2021.09.187. Online ahead of print.ABSTRACTPumpkin starch (PS) was extracted from Cucurbita maxima and utilized to prepare films in combination with cellulose nanocrystal (CNC) and cellulose nanofiber (CNF), using a solvent casting strategy. The PS was characterized to contain 26.6% of amylose, exhibiting a "B"-type crystalline structure and high stability against thermal degradation. PS/CNF films showed better thermal stability than PS/CNC films, whereas the CNC was more effective than CNF for enhancing the tensile strength (TS) of the films. T...
Source: International Journal of Biological Macromolecules - October 4, 2021 Category: Biochemistry Authors: Luyao Zhang Jing Zhao Yu Zhang Fei Li Xu Jiao Quanhong Li Source Type: research

The effects of cellulose nanocrystal and cellulose nanofiber on the properties of pumpkin starch-based composite films
Int J Biol Macromol. 2021 Oct 1:S0141-8130(21)02115-2. doi: 10.1016/j.ijbiomac.2021.09.187. Online ahead of print.ABSTRACTPumpkin starch (PS) was extracted from Cucurbita maxima and utilized to prepare films in combination with cellulose nanocrystal (CNC) and cellulose nanofiber (CNF), using a solvent casting strategy. The PS was characterized to contain 26.6% of amylose, exhibiting a "B"-type crystalline structure and high stability against thermal degradation. PS/CNF films showed better thermal stability than PS/CNC films, whereas the CNC was more effective than CNF for enhancing the tensile strength (TS) of the films. T...
Source: International Journal of Biological Macromolecules - October 4, 2021 Category: Biochemistry Authors: Luyao Zhang Jing Zhao Yu Zhang Fei Li Xu Jiao Quanhong Li Source Type: research

The Fourth Quarter is Ours
It is October, the first month of the fourth quarter of the year. Fall has arrived, and the weather where you are may be changing. Local stores have likely updated their inventory from fall d écor to that of the upcoming holidays celebrated in the final 2 months of the year. Local coffee shops are likely filled with the aroma of pumpkin spiced lattes and other delightful pumpkin-based treats. For some of us, it brings the joy of one of America’s favorite past-times, football. (Source: The Journal for Nurse Practitioners)
Source: The Journal for Nurse Practitioners - October 4, 2021 Category: Nursing Authors: Courtney J. Pitts Tags: Editorial Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | PMC:PMC8576748 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | PMC:PMC8576748 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research

The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier < em > in vitro < /em >
In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.PMID:34587668 | DOI:10.2478/aiht-2021-72-3541 (Source: Arhiv za Higijenu Rada i Toksikologiju)
Source: Arhiv za Higijenu Rada i Toksikologiju - September 29, 2021 Category: Occupational Health Authors: Manuel Alonso-Garrido Noelia Pallar és Guillermina Font Paola Tedeschi Lara Manyes Manuel Lozano Source Type: research