Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Herbal extracts that induce type I interferons through Toll-like receptor 4 signaling
CONCLUSIONS: INT induced type I IFN mRNA and proteins in BMDMs and other cell types. This induction was mediated by TLR4, which transduces signals using the TRIF/MyD88 pathway. The high-MW component of INT contained type I IFN inducing activity. The supernatants from INT-treated cells displayed antiviral activity and protected cells from EMCV infection. These findings indicate that INT is a novel natural IFN inducer that strengthens host's innate immunity.PMID:35173566 | PMC:PMC8809074 | DOI:10.29219/fnr.v66.5524 (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - February 17, 2022 Category: Nutrition Authors: Misa Nakasuji-Togi Sumihito Togi Keita Saeki Yasuhiko Kojima Keiko Ozato Source Type: research
Evaluation of Some Agro-Industrial Wastes as Fermentation Medium for Pullulan Production by Aureobasidium pullulans AZ-6
In this study, different agro-industrial wastes; various citrus peels, grape pomace, the hydrolysates of hazelnut and chestnut shells, sugarcane molasses residue, dried and fresh hazelnut husks and pumpkin peel, were used as fermentation media without adding any extra nutritional component for pullulan production by A. pullulans AZ-6. As a result, among the tested media, the maximum pullulan concentration was obtained as 33.59 gL-1 using the sugarcane molasses residue, and followed by the corresponding value of 30.02 gL-1 obtained in the dried hazelnut husk hydrolysate medium. Therefore, the usage of agro-industrial wastes...
Source: Current Microbiology - February 9, 2022 Category: Microbiology Authors: B üşra Akdeniz Oktay M Tijen Bozdemir Z Ye şim Özbaş Source Type: research
Evaluation of Some Agro-Industrial Wastes as Fermentation Medium for Pullulan Production by Aureobasidium pullulans AZ-6
In this study, different agro-industrial wastes; various citrus peels, grape pomace, the hydrolysates of hazelnut and chestnut shells, sugarcane molasses residue, dried and fresh hazelnut husks and pumpkin peel, were used as fermentation media without adding any extra nutritional component for pullulan production by A. pullulans AZ-6. As a result, among the tested media, the maximum pullulan concentration was obtained as 33.59 gL-1 using the sugarcane molasses residue, and followed by the corresponding value of 30.02 gL-1 obtained in the dried hazelnut husk hydrolysate medium. Therefore, the usage of agro-industrial wastes...
Source: Current Microbiology - February 9, 2022 Category: Microbiology Authors: B üşra Akdeniz Oktay M Tijen Bozdemir Z Ye şim Özbaş Source Type: research
In vivo pharmacokinetic study of a Cucurbita moschata polysaccharide after oral administration
This study aimed to elucidate the pharmacokinetic information (the plasma concentration-time curve, tissue distribution, and excretion profiles) of the pumpkin polysaccharide (PPc) in vivo after oral administration. Results revealed that PPc could enter into the blood and exhibited a relatively long circulation in the blood with a mean residence time (MRT) of 7.45 h and presented a higher aggregation of PPc in the liver and kidneys. To obtain the visualization of the systemic circulation of PPc, in vivo imaging was used with near-infrared fluorescence (NIRF) imaging, which distributed into various tissues with different re...
Source: International Journal of Biological Macromolecules - January 29, 2022 Category: Biochemistry Authors: Fei Li Yunlu Wei Jing Zhao Luyao Zhang Quanhong Li Source Type: research
In vivo pharmacokinetic study of a Cucurbita moschata polysaccharide after oral administration
This study aimed to elucidate the pharmacokinetic information (the plasma concentration-time curve, tissue distribution, and excretion profiles) of the pumpkin polysaccharide (PPc) in vivo after oral administration. Results revealed that PPc could enter into the blood and exhibited a relatively long circulation in the blood with a mean residence time (MRT) of 7.45 h and presented a higher aggregation of PPc in the liver and kidneys. To obtain the visualization of the systemic circulation of PPc, in vivo imaging was used with near-infrared fluorescence (NIRF) imaging, which distributed into various tissues with different re...
Source: International Journal of Biological Macromolecules - January 29, 2022 Category: Biochemistry Authors: Fei Li Yunlu Wei Jing Zhao Luyao Zhang Quanhong Li Source Type: research
Genes, Vol. 13, Pages 262: Genome-Wide Identification of CCD Gene Family in Six Cucurbitaceae Species and Its Expression Profiles in Melon
This study presents a comprehensive analysis of CCD gene family in six species of Cucurbitaceae, providing a strong foundation for future studies on specific genes in this family. (Source: Genes)
Source: Genes - January 28, 2022 Category: Genetics & Stem Cells Authors: Cheng Wang Li Zhao Wei Zhang Tags: Article Source Type: research
Long-distance control of pumpkin rootstock over cucumber scion under drought stress as revealed by transcriptome sequencing and mobile mRNAs identifications
Hortic Res. 2022 Jan 19:uhab033. doi: 10.1093/hr/uhab033. Online ahead of print.ABSTRACTGrafting with pumpkin rootstock is commonly used not only to improve the quality of cucumber fruits but also to confer biotic or abiotic stress tolerance. However, the molecular mechanism of grafted cucumbers to drought stress and the possible roles of mobile mRNAs to improve stress tolerance have remained obscure. Hence, we conducted transcriptome sequencing and combined it with morpho-physiological experiments to compare the response of homografts (cucumber as scion and rootstock) (C) and heterografts (cucumber as scion and pumpkin as...
Source: Cell Research - January 19, 2022 Category: Cytology Authors: Marzieh Davoudi Mengfei Song Mengru Zhang Jinfeng Chen Qunfeng Lou Source Type: research
Pumpkin seed oil and zinc attenuate chronic mild stress perturbations in the cerebral cortex of rats
This study aims to investigate the potential of pumpkin seed oil (PSO) and zinc to attenuate oxidative stress and neuroinflammation caused by chronic mild stress (CMS) in the cerebral cortex of male rats.
The rats were submitted to stress for six weeks and then the behavior of the rats was tested by forced swimming test (FST) and novel cage test. The treated groups were given venlafaxine (20 mg/kg), pumpkin seed oil (40 mg/kg) and zinc (4 mg/kg). The cortex homogenate was used for the detection of the oxidative stress parameters, the concentration of neurotransmitters, tumor necrosis factor-α (TNF-α) and inter...
Source: Nutrition and Food Science - January 12, 2022 Category: Nutrition Authors: Marwa H. El-Azma
Nadia M. El-Beih
Karima A. El-Shamy
Khaled M.M. Koriem
Mahitab I. Elkassaby
Wael M. El-Sayed Source Type: research
Efficient virus-induced gene silencing system in pumpkin (Cucurbita maxima) using apple latent spherical virus vector
J Virol Methods. 2022 Jan 6;301:114456. doi: 10.1016/j.jviromet.2022.114456. Online ahead of print.ABSTRACTCrude-sap of apple latent spherical virus (ALSV)-infected Chenopodium quinoa leaves was rub-inoculated on the expanded cotyledons of various Cucurbitaceae plants. Most of the species were systemically infected with the virus without obvious symptoms, except pumpkin (Cucurbita maxima). In pumpkin, the ALSV infection was restricted to inoculated cotyledons; it did not spread to the upper true leaves. In situ hybridization showed that the ALSV was confined to part of the cotyledon tissues and it did not invade the phloem...
Source: Journal of Virological Methods - January 9, 2022 Category: Virology Authors: Noriko Yamagishi Nobuyuki Yoshikawa Source Type: research
