GSE264574 Understanding resistance to polymyxin and amikacin in Klebsiella pneumoniae
Contributors : Camila de Souza ; Octavio FrancoSeries Type : Expression profiling by high throughput sequencingOrganism : Klebsiella pneumoniaeKlebsiella pneumoniae poses a significant global health threat primarily attributable to its pronounced resistance. Here, we report an in vitro acquired resistance analyses of K. pneumoniae to the combination of amikacin and polymyxin B. We found some differentially expressed genes associated with the resistome of K. pneumoniae. The main differences were found in the genes aphA, asmA, phoP, and in the arn operon. Once these genes are related to modification in lipopolysaccharides,...
Source: GEO: Gene Expression Omnibus - April 26, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Klebsiella pneumoniae Source Type: research
The NLRP3 Inflammasome Is a Major Cause of Acute Renal Failure Induced by Polypeptide Antibiotics
J Immunol. 2024 Apr 19:ji2300193. doi: 10.4049/jimmunol.2300193. Online ahead of print.ABSTRACTDrug-induced acute renal failure (ARF) is a public health concern that hinders optimal drug therapy. However, pathological mechanisms of drug-induced ARF remain to be elucidated. Here, we show that a pathological process of drug-induced ARF is mediated by proinflammatory cross-talk between kidney tubular cells and macrophages. Both polymyxin B and colistin, polypeptide antibiotics, frequently cause ARF, stimulated the ERK and NF-κB pathways in kidney tubular cells, and thereby upregulated M-CSF and MCP-1, leading to infiltration...
Source: Journal of Immunology - April 19, 2024 Category: Allergy & Immunology Authors: Tomohiro Kagi Aya Inoue Takuya Noguchi Wakana Suzuki Saya Takano Kohei Otani Rio Naganuma Yuto Sekiguchi Yusuke Hirata Sawako Shindo Gi-Wook Hwang Atsushi Matsuzawa Source Type: research
Paper-based fluorescence sensor array with functionalized carbon quantum dots for bacterial discrimination using a machine learning algorithm
Anal Bioanal Chem. 2024 Apr 17. doi: 10.1007/s00216-024-05262-4. Online ahead of print.ABSTRACTThe rapid discrimination of bacteria is currently an emerging trend in the fields of food safety, medical detection, and environmental observation. Traditional methods often require lengthy culturing processes, specialized analytical equipment, and bacterial recognition receptors. In response to this need, we have developed a paper-based fluorescence sensor array platform for identifying different bacteria. The sensor array is based on three unique carbon quantum dots (CQDs) as sensing units, each modified with a different antibi...
Source: Analytical and Bioanalytical Chemistry - April 17, 2024 Category: Chemistry Authors: Fangbin Wang Minghui Xiao Jing Qi Liang Zhu Source Type: research
A systematic evaluation of population pharmacokinetic models for polymyxin B in patients with liver and/or kidney dysfunction
This study aimed to systemically evaluate eleven PMB PopPK models from ten published literature based on a new independent population, which was divided into four different populations, patients with liver dysfunction, kidney dysfunction, liver and kidney dysfunction, and normal liver and kidney function. The whole data set consisted of 146 patients with 391 PMB concentrations. The prediction- and simulation-based diagnostics and Bayesian forecasting were conducted to evaluate model predictability. In the overall evaluation process, none of the models exhibited satisfactory predictive ability in both prediction- and simula...
Source: Journal of Pharmacokinetics and Pharmacodynamics - April 16, 2024 Category: Drugs & Pharmacology Source Type: research
GSE262848 High-throughput Fitness Experiments Reveal Specific Vulnerabilities of Human-Adapted Salmonella During Stress and Infection- Barseq9 (LB, gentamycin, cotrimoxazole, rifampicin, polymyxin B)
Contributors : Benjamin X Wang ; Denise M MonackSeries Type : OtherOrganism : Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150 ; Salmonella enterica subsp. enterica serovar Typhi str. Ty2 ; Salmonella enterica subsp. enterica serovar Typhimurium str. D23580 ; Salmonella enterica subsp. enterica serovar Typhimurium str. ST4/74Salmonella enterica is comprised of genetically distinct “serovars”, that together provide an intriguing model for exploring the genetic basis of pathogen evolution. While the genomes of numerous Salmonella isolates with broad variations in host range and human disease manifes...
Source: GEO: Gene Expression Omnibus - April 12, 2024 Category: Genetics & Stem Cells Tags: Other Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150 Salmonella enterica subsp. enterica serovar Typhi str. Ty2 Salmonella enterica subsp. enterica serovar Typhimurium str. D23580 Salmonella enterica subsp. enterica serovar Source Type: research
Discovery of a novel class of rosmarinic acid derivatives as antibacterial agents: Synthesis, structure-activity relationship and mechanism of action
Bioorg Chem. 2024 Mar 28;146:107318. doi: 10.1016/j.bioorg.2024.107318. Online ahead of print.ABSTRACTTwenty-seven rosmarinic acid derivatives were synthesized, among which compound RA-N8 exhibited the most potent antibacterial ability. The minimum inhibition concentration of RA-N8 against both S. aureus (ATCC 29213) and MRSA (ATCC BAA41 and ATCC 43300) was found to be 6 μg/mL, and RA-N8 killed E. coli (ATCC 25922) at 3 μg/mL in the presence of polymyxin B nonapeptide (PMBN) which increased the permeability of E. coli. RA-N8 exhibited a weak hemolytic effect at the minimum inhibitory concentration. SYTOX Green assay, SEM...
Source: Bioorganic Chemistry - April 5, 2024 Category: Chemistry Authors: Yong Wang Zhiguang Liang Yihui Cao Cheung-Hin Hung Ruolan Du Alan Siu-Lun Leung Pui-Kin So Pak-Ho Chan Wing-Leung Wong Yun-Chung Leung Kwok-Yin Wong Source Type: research
Discovery of a novel class of rosmarinic acid derivatives as antibacterial agents: Synthesis, structure-activity relationship and mechanism of action
Bioorg Chem. 2024 Mar 28;146:107318. doi: 10.1016/j.bioorg.2024.107318. Online ahead of print.ABSTRACTTwenty-seven rosmarinic acid derivatives were synthesized, among which compound RA-N8 exhibited the most potent antibacterial ability. The minimum inhibition concentration of RA-N8 against both S. aureus (ATCC 29213) and MRSA (ATCC BAA41 and ATCC 43300) was found to be 6 μg/mL, and RA-N8 killed E. coli (ATCC 25922) at 3 μg/mL in the presence of polymyxin B nonapeptide (PMBN) which increased the permeability of E. coli. RA-N8 exhibited a weak hemolytic effect at the minimum inhibitory concentration. SYTOX Green assay, SEM...
Source: Bioorganic Chemistry - April 5, 2024 Category: Chemistry Authors: Yong Wang Zhiguang Liang Yihui Cao Cheung-Hin Hung Ruolan Du Alan Siu-Lun Leung Pui-Kin So Pak-Ho Chan Wing-Leung Wong Yun-Chung Leung Kwok-Yin Wong Source Type: research
Retracted: In Vivo Development of Polymyxin B Resistance in < em > Klebsiella pneumoniae < /em > owing to a 42 bp Deletion in the Sequence of < em > phoQ < /em >
Biomed Res Int. 2024 Mar 20;2024:9791312. doi: 10.1155/2024/9791312. eCollection 2024.ABSTRACT[This retracts the article DOI: 10.1155/2020/5868479.].PMID:38550180 | PMC:PMC10973224 | DOI:10.1155/2024/9791312 (Source: Biomed Res)
Source: Biomed Res - March 29, 2024 Category: Research Authors: BioMed Research International Source Type: research
Retracted: In Vivo Development of Polymyxin B Resistance in < em > Klebsiella pneumoniae < /em > owing to a 42 bp Deletion in the Sequence of < em > phoQ < /em >
Biomed Res Int. 2024 Mar 20;2024:9791312. doi: 10.1155/2024/9791312. eCollection 2024.ABSTRACT[This retracts the article DOI: 10.1155/2020/5868479.].PMID:38550180 | PMC:PMC10973224 | DOI:10.1155/2024/9791312 (Source: Biomed Res)
Source: Biomed Res - March 29, 2024 Category: Research Authors: BioMed Research International Source Type: research
Sensors, Vol. 24, Pages 2169: Molecular Weights of Polyethyleneimine-Dependent Physicochemical Tuning of Gold Nanoparticles and FRET-Based Turn-On Sensing of Polymyxin B
. Narayan
Environmental monitoring and the detection of antibiotic contaminants require expensive and time-consuming techniques. To overcome these challenges, gold nanoparticle-mediated fluorometric &ldquo;turn-on&rdquo; detection of Polymyxin B (PMB) in an aqueous medium was undertaken. The molecular weight of polyethyleneimine (PEI)-dependent physicochemical tuning of gold nanoparticles (PEI@AuNPs) was achieved and employed for the same. The three variable molecular weights of branched polyethyleneimine (MW 750, 60, and 1.3 kDa) molecules controlled the nano-geometry of the gold nanoparticles along wi...
Source: Sensors - March 28, 2024 Category: Biotechnology Authors: Atul Kumar Tiwari Munesh Kumar Gupta Ramovatar Meena Prem C. Pandey Roger J. Narayan Tags: Article Source Type: research
Detection and characterization of eravacycline heteroresistance in clinical bacterial isolates
This study aimed to investigate ERV HR prevalence among common clinical isolates and further characterize ERV HR in carbapenem-resistant Klebsiella pneumoniae (CRKP). A total of 280 clinical pathogens from two centers were selected for HR and analyzed using population analysis profiling (PAP) and modified E-tests. The PAP assay revealed an overall ERV HR prevalence of 0.7% (2/280), with intermediate heterogeneity observed in 24.3% (68/280) of strains. The proportion of heteroresistant strains was 18.3% according to modified E-test results. A time-killing assay demonstrated that CRKP CFU increased significantly after 10 h...
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
β-lactam-induced OMV release promotes polymyxin tolerance in Salmonella enterica sv. Typhi
This study emphasizes the importance of evaluating the influence of β-lactam antibiotics on the interaction between OMVs and other antimicrobial agents. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 25, 2024 Category: Microbiology Source Type: research
Suppression of planktonic and biofilm of Escherichia coli by the synergistic lantibiotics-polymyxins combinations
Arch Microbiol. 2024 Mar 23;206(4):191. doi: 10.1007/s00203-024-03922-8.ABSTRACTEscherichia coli are generally resistant to the lantibiotic's action (nisin and warnerin), but we have shown increased sensitivity of E. coli to lantibiotics in the presence of subinhibitory concentrations of polymyxins. Synergistic lantibiotic-polymyxin combinations were found for polymyxins B and M. The killing of cells at the planktonic and biofilm levels was observed for two collection and four clinical multidrug-resistant E. coli strains after treatment with lantibiotic-polymyxin B combinations. Thus, 24-h treatment of E. coli mature biofi...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Tatyana Polyudova Larisa Lemkina Daria Eroshenko Artem Esaev Source Type: research
Suppression of planktonic and biofilm of Escherichia coli by the synergistic lantibiotics-polymyxins combinations
Arch Microbiol. 2024 Mar 23;206(4):191. doi: 10.1007/s00203-024-03922-8.ABSTRACTEscherichia coli are generally resistant to the lantibiotic's action (nisin and warnerin), but we have shown increased sensitivity of E. coli to lantibiotics in the presence of subinhibitory concentrations of polymyxins. Synergistic lantibiotic-polymyxin combinations were found for polymyxins B and M. The killing of cells at the planktonic and biofilm levels was observed for two collection and four clinical multidrug-resistant E. coli strains after treatment with lantibiotic-polymyxin B combinations. Thus, 24-h treatment of E. coli mature biofi...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Tatyana Polyudova Larisa Lemkina Daria Eroshenko Artem Esaev Source Type: research
