Structural and biochemical analysis of penicillin-binding protein 2 from Campylobacter jejuni
Biochem Biophys Res Commun. 2024 Mar 30;710:149859. doi: 10.1016/j.bbrc.2024.149859. Online ahead of print.ABSTRACTPenicillin-binding protein 2 (PBP2) plays a key role in the formation of peptidoglycans in bacterial cell walls by crosslinking glycan chains through transpeptidase activity. PBP2 is also found in Campylobacter jejuni, a pathogenic bacterium that causes food-borne enteritis in humans. To elucidate the essential structural features of C. jejuni PBP2 (cjPBP2) that mediate its biological function, we determined the crystal structure of cjPBP2 and assessed its protein stability under various conditions. cjPBP2 ado...
Source: Biochemical and Biophysical Research communications - April 6, 2024 Category: Biochemistry Authors: Hong Joon Choi Dong Uk Ki Sung-Il Yoon Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Structural and biochemical analysis of penicillin-binding protein 2 from Campylobacter jejuni
Biochem Biophys Res Commun. 2024 Mar 30;710:149859. doi: 10.1016/j.bbrc.2024.149859. Online ahead of print.ABSTRACTPenicillin-binding protein 2 (PBP2) plays a key role in the formation of peptidoglycans in bacterial cell walls by crosslinking glycan chains through transpeptidase activity. PBP2 is also found in Campylobacter jejuni, a pathogenic bacterium that causes food-borne enteritis in humans. To elucidate the essential structural features of C. jejuni PBP2 (cjPBP2) that mediate its biological function, we determined the crystal structure of cjPBP2 and assessed its protein stability under various conditions. cjPBP2 ado...
Source: Cell Research - April 6, 2024 Category: Cytology Authors: Hong Joon Choi Dong Uk Ki Sung-Il Yoon Source Type: research
Structural and biochemical analysis of penicillin-binding protein 2 from Campylobacter jejuni
Biochem Biophys Res Commun. 2024 Mar 30;710:149859. doi: 10.1016/j.bbrc.2024.149859. Online ahead of print.ABSTRACTPenicillin-binding protein 2 (PBP2) plays a key role in the formation of peptidoglycans in bacterial cell walls by crosslinking glycan chains through transpeptidase activity. PBP2 is also found in Campylobacter jejuni, a pathogenic bacterium that causes food-borne enteritis in humans. To elucidate the essential structural features of C. jejuni PBP2 (cjPBP2) that mediate its biological function, we determined the crystal structure of cjPBP2 and assessed its protein stability under various conditions. cjPBP2 ado...
Source: Biochemical and Biophysical Research communications - April 6, 2024 Category: Biochemistry Authors: Hong Joon Choi Dong Uk Ki Sung-Il Yoon Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Chemical biology tools to probe bacterial glycans
Curr Opin Chem Biol. 2024 Apr 5;80:102453. doi: 10.1016/j.cbpa.2024.102453. Online ahead of print.ABSTRACTBacterial cells are covered by a complex carbohydrate coat of armor that allows bacteria to thrive in a range of environments. As a testament to the importance of bacterial glycans, effective and heavily utilized antibiotics including penicillin and vancomycin target and disrupt the bacterial glycocalyx. Despite their importance, the study of bacterial glycans lags far behind their eukaryotic counterparts. Bacterial cells use a large palette of monosaccharides to craft glycans, leading to molecules that are significant...
Source: Current Opinion in Chemical Biology - April 6, 2024 Category: Biochemistry Authors: Daniel Calles-Garcia Danielle H Dube Source Type: research
Antimicrobial Resistance Patterns in Patients with Vaginal Discharge: A 2019-2022 Analysis at the National Health Laboratory in Eritrea
CONCLUSION: Most gram-negative bacteria displayed higher resistance of >45% to ampicillin, trimethoprim-sulfamethoxazole, and tetracycline. Among gram-positive bacteria, a higher resistance rate with ampicillin, trimethoprim-sulfamethoxazole, tetracycline, oxacillin, vancomycin, and penicillin G was recorded. S. aureus showed no resistance to rifampicin and clindamycin, and Strep. spp. indicated 100% resistance to rifampicin and vancomycin. This study highlights critical gaps and areas for further exploration. Expanding the spectrum of antibiotics tested and investigating underlying multidrug resistance mechanisms would...
Source: Biomed Res - April 5, 2024 Category: Research Authors: Khalid Hussein Berhe Tesfai Okbu Frezgi Hagos Hayelom Yosan Gebremeskel Azania Werede Hailemichael Gebremariam Fitsum Kibreab Mohammed Elfatih Hamida Source Type: research
Antimicrobial Resistance Patterns in Patients with Vaginal Discharge: A 2019-2022 Analysis at the National Health Laboratory in Eritrea
CONCLUSION: Most gram-negative bacteria displayed higher resistance of >45% to ampicillin, trimethoprim-sulfamethoxazole, and tetracycline. Among gram-positive bacteria, a higher resistance rate with ampicillin, trimethoprim-sulfamethoxazole, tetracycline, oxacillin, vancomycin, and penicillin G was recorded. S. aureus showed no resistance to rifampicin and clindamycin, and Strep. spp. indicated 100% resistance to rifampicin and vancomycin. This study highlights critical gaps and areas for further exploration. Expanding the spectrum of antibiotics tested and investigating underlying multidrug resistance mechanisms would...
Source: Biomed Res - April 5, 2024 Category: Research Authors: Khalid Hussein Berhe Tesfai Okbu Frezgi Hagos Hayelom Yosan Gebremeskel Azania Werede Hailemichael Gebremariam Fitsum Kibreab Mohammed Elfatih Hamida Source Type: research
Antimicrobial Resistance Patterns in Patients with Vaginal Discharge: A 2019-2022 Analysis at the National Health Laboratory in Eritrea
CONCLUSION: Most gram-negative bacteria displayed higher resistance of >45% to ampicillin, trimethoprim-sulfamethoxazole, and tetracycline. Among gram-positive bacteria, a higher resistance rate with ampicillin, trimethoprim-sulfamethoxazole, tetracycline, oxacillin, vancomycin, and penicillin G was recorded. S. aureus showed no resistance to rifampicin and clindamycin, and Strep. spp. indicated 100% resistance to rifampicin and vancomycin. This study highlights critical gaps and areas for further exploration. Expanding the spectrum of antibiotics tested and investigating underlying multidrug resistance mechanisms would...
Source: Biomed Res - April 5, 2024 Category: Research Authors: Khalid Hussein Berhe Tesfai Okbu Frezgi Hagos Hayelom Yosan Gebremeskel Azania Werede Hailemichael Gebremariam Fitsum Kibreab Mohammed Elfatih Hamida Source Type: research
Antimicrobial Resistance Patterns in Patients with Vaginal Discharge: A 2019-2022 Analysis at the National Health Laboratory in Eritrea
CONCLUSION: Most gram-negative bacteria displayed higher resistance of >45% to ampicillin, trimethoprim-sulfamethoxazole, and tetracycline. Among gram-positive bacteria, a higher resistance rate with ampicillin, trimethoprim-sulfamethoxazole, tetracycline, oxacillin, vancomycin, and penicillin G was recorded. S. aureus showed no resistance to rifampicin and clindamycin, and Strep. spp. indicated 100% resistance to rifampicin and vancomycin. This study highlights critical gaps and areas for further exploration. Expanding the spectrum of antibiotics tested and investigating underlying multidrug resistance mechanisms would...
Source: Biomed Res - April 5, 2024 Category: Research Authors: Khalid Hussein Berhe Tesfai Okbu Frezgi Hagos Hayelom Yosan Gebremeskel Azania Werede Hailemichael Gebremariam Fitsum Kibreab Mohammed Elfatih Hamida Source Type: research
Sociodemographic and clinical characteristics associated with maternal and congenital syphilis-A prospective study in Peru
Syphilis, one of the oldest known infectious diseases, is caused by a spirochetal pathogen Treponema pallidum subspecies pallidum (T. pallidum henceforth). The World Health Organization (WHO) estimates a global prevalence of 36 million cases of syphilis, with 6-12 million new infections occurring annually. Syphilis is an easily treatable disease because parenteral treatment with Benzathine penicillin G usually clears T. pallidum infection. Yet, inaccessibility to proper testing and treatment facilities in resource limited regions remains the major cause of the high incidence of syphilis around the world. (Source: Internati...
Source: International Journal of Infectious Diseases - April 5, 2024 Category: Infectious Diseases Authors: Cesar P Carcamo, Carlos Velasquez, Sandra C. Rocha, Arturo Centurion-Lara, Lorena Lopez-Torres, Nikhat Parveen Source Type: research
