The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Atransgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis
Transgenic Res. 2023 May 3. doi: 10.1007/s11248-023-00349-7. Online ahead of print.ABSTRACTMaintenance of calcium homeostasis is important for proper endoplasmic reticulum (ER) function. When cellular stress conditions deplete the high concentration of calcium in the ER, ER-resident proteins are secreted into the extracellular space in a process called exodosis. Monitoring exodosis provides insight into changes in ER homeostasis and proteostasis resulting from cellular stress associated with ER calcium dysregulation. To monitor cell-type specific exodosis in the intact animal, we created a transgenic mouse line with a Gaus...
Source: Transgenic Research - May 3, 2023 Category: Genetics & Stem Cells Authors: Reinis Svarcbahs Sarah M Blossom Helena S Baffoe-Bonnie Kathleen A Trychta Lacey K Greer James Pickel Mark J Henderson Brandon K Harvey Source Type: research
Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin
Transgenic Res. 2023 May 2. doi: 10.1007/s11248-023-00350-0. Online ahead of print.ABSTRACTPlant defensins are a potential tool in crop improvement programs through biotechnology. Their antifungal action makes them attractive molecules for the production of transgenic plants. Information is currently lacking on what happens to the expression of defense genes in transgenic plants that overexpress a defensin. Here we show the relative expression of four defense-related genes: Mn-sod, PAL1, aos1 and HPL evaluated in two transgenic soybean events (Def1 and Def17) constitutively expressing the NmDef02 defensin gene from Nicotia...
Source: Transgenic Research - May 2, 2023 Category: Genetics & Stem Cells Authors: Alejandro E Morales Natacha Soto Celia Delgado Yuniet Hern ández Leonardo Carrillo Camilo Ferrero Gil A Enr íquez Source Type: research
Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'
In this study, a stable Agrobacterium mediated genetic transformation system of Juglans sigillata Dode 'Qianhe-7' was investigated using callus and negative pressure-assisted and ultrasonic-assisted transformation selection. The results showed that the axillary shoot leaves were suitable to induce callus and the callus proliferation rate could reach 516.27% when induction calli were cultured on DKW medium containing 0.5 mg L-1 indole-3-butyric acid, 1.2 mg L-1 2,4-dichlorophenoxyacetic acid and 0.5 mg L-1 kinetin for 18 d. In addition, negative pressure infection was the optimal infection method with the lowest browning ra...
Source: Transgenic Research - April 28, 2023 Category: Genetics & Stem Cells Authors: Rong Wei Wen'e Zhang Chunxiang Li Zhenkun Hao Dong Huang Wenlong Zhang Xuejun Pan Source Type: research
Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'
In this study, a stable Agrobacterium mediated genetic transformation system of Juglans sigillata Dode 'Qianhe-7' was investigated using callus and negative pressure-assisted and ultrasonic-assisted transformation selection. The results showed that the axillary shoot leaves were suitable to induce callus and the callus proliferation rate could reach 516.27% when induction calli were cultured on DKW medium containing 0.5 mg L-1 indole-3-butyric acid, 1.2 mg L-1 2,4-dichlorophenoxyacetic acid and 0.5 mg L-1 kinetin for 18 d. In addition, negative pressure infection was the optimal infection method with the lowest browning ra...
Source: Transgenic Research - April 28, 2023 Category: Genetics & Stem Cells Authors: Rong Wei Wen'e Zhang Chunxiang Li Zhenkun Hao Dong Huang Wenlong Zhang Xuejun Pan Source Type: research
Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'
In this study, a stable Agrobacterium mediated genetic transformation system of Juglans sigillata Dode 'Qianhe-7' was investigated using callus and negative pressure-assisted and ultrasonic-assisted transformation selection. The results showed that the axillary shoot leaves were suitable to induce callus and the callus proliferation rate could reach 516.27% when induction calli were cultured on DKW medium containing 0.5 mg L-1 indole-3-butyric acid, 1.2 mg L-1 2,4-dichlorophenoxyacetic acid and 0.5 mg L-1 kinetin for 18 d. In addition, negative pressure infection was the optimal infection method with the lowest browning ra...
Source: Transgenic Research - April 28, 2023 Category: Genetics & Stem Cells Authors: Rong Wei Wen'e Zhang Chunxiang Li Zhenkun Hao Dong Huang Wenlong Zhang Xuejun Pan Source Type: research
