Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice
In this study, we have reported a novel gene transfer protocol that involves pulling out primary leaf from coleoptile and injection of Agrobacterium culture into the empty channel. Out of 25 plants survived after injection of Agrobacterium tumefaciens EHA105 culture harboring pCAMBIA1301-RD29A-AtDREB1A, 8 T0 plants revealed the expected size of around 811 bp corresponding to AtDREB1A gene and Southern blotting analysis on 18 T1 plants suggested introgression of AtDREB1A. 3 T2 lines (7-9, 12-3, 18-6) exhibited accumulation of free proline and soluble sugars, yet increase of chlorophyll content, but decrease of electrolyte l...
Source: Transgenic Research - June 15, 2023 Category: Genetics & Stem Cells Authors: Un-Hyang Ho Sam-Rang Song Yong-Il Choe Myong-Hyok Pak Mi-Hyang Kim Kang Kim Tong-Su Ho Source Type: research
Development of Agrobacterium-mediated in planta transformation protocol through coleoptile in rice
In this study, we have reported a novel gene transfer protocol that involves pulling out primary leaf from coleoptile and injection of Agrobacterium culture into the empty channel. Out of 25 plants survived after injection of Agrobacterium tumefaciens EHA105 culture harboring pCAMBIA1301-RD29A-AtDREB1A, 8 T0 plants revealed the expected size of around 811 bp corresponding to AtDREB1A gene and Southern blotting analysis on 18 T1 plants suggested introgression of AtDREB1A. 3 T2 lines (7-9, 12-3, 18-6) exhibited accumulation of free proline and soluble sugars, yet increase of chlorophyll content, but decrease of electrolyte l...
Source: Transgenic Research - June 15, 2023 Category: Genetics & Stem Cells Authors: Un-Hyang Ho Sam-Rang Song Yong-Il Choe Myong-Hyok Pak Mi-Hyang Kim Kang Kim Tong-Su Ho Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
GEnZ explorer: a tool for visualizing agroclimate to inform research and regulatory risk assessment
Transgenic Res. 2023 Jun 6. doi: 10.1007/s11248-023-00354-w. Online ahead of print.ABSTRACTConfined field trials (CFT) of genetically engineered (GE) crops are used to generate data to inform environmental risk assessments (ERA). ERAs are required by regulatory authorities before novel GE crops can be released for cultivation. The transportability of CFT data to inform risk assessment in countries other than those where the CFT was conducted has been discussed previously in an analysis showing that the primary difference between CFT locations potentially impacting trial outcomes is the physical environment, particularly th...
Source: Transgenic Research - June 6, 2023 Category: Genetics & Stem Cells Authors: Rachel L Melnick Larissa Jarvis Paul Hendley Monica Garcia-Alonso Marc J Metzger Navin Ramankutty John L Teem Andrew Roberts Source Type: research
A novel approach to achieving more efficient production of the mature form of human IL-37 in plants
Transgenic Res. 2023 Jun 2. doi: 10.1007/s11248-023-00351-z. Online ahead of print.ABSTRACTInterleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01...
Source: Transgenic Research - June 2, 2023 Category: Genetics & Stem Cells Authors: Yao Zhang Nadiyah Alqazlan Zihe Meng Jingyao Zhao Nan Liu Yuxin Zhang Mingfeng Feng Shengwu Ma Aoxue Wang Source Type: research
A novel approach to achieving more efficient production of the mature form of human IL-37 in plants
Transgenic Res. 2023 Jun 2. doi: 10.1007/s11248-023-00351-z. Online ahead of print.ABSTRACTInterleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01...
Source: Transgenic Research - June 2, 2023 Category: Genetics & Stem Cells Authors: Yao Zhang Nadiyah Alqazlan Zihe Meng Jingyao Zhao Nan Liu Yuxin Zhang Mingfeng Feng Shengwu Ma Aoxue Wang Source Type: research
A novel approach to achieving more efficient production of the mature form of human IL-37 in plants
Transgenic Res. 2023 Jun 2. doi: 10.1007/s11248-023-00351-z. Online ahead of print.ABSTRACTInterleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01...
Source: Transgenic Research - June 2, 2023 Category: Genetics & Stem Cells Authors: Yao Zhang Nadiyah Alqazlan Zihe Meng Jingyao Zhao Nan Liu Yuxin Zhang Mingfeng Feng Shengwu Ma Aoxue Wang Source Type: research
A novel approach to achieving more efficient production of the mature form of human IL-37 in plants
Transgenic Res. 2023 Jun 2. doi: 10.1007/s11248-023-00351-z. Online ahead of print.ABSTRACTInterleukin-37 is a newly discovered cytokine that plays a pivotal role in suppressing innate inflammation and acquired immunity. We have recently expressed both the mature(mat-) and pro-forms of human IL-37b in plants and demonstrated that while both forms of the plant-made hIL-37b are functional, pmat-hIL37b exhibited significantly greater activity than ppro-IL-37b. Compared to ppro-hIL-37b, on the other hand, the expression level of pmat-hIL-37b was substantially lower (100.5 µg versus 1.05 µg/g fresh leaf mass or 1% versus 0.01...
Source: Transgenic Research - June 2, 2023 Category: Genetics & Stem Cells Authors: Yao Zhang Nadiyah Alqazlan Zihe Meng Jingyao Zhao Nan Liu Yuxin Zhang Mingfeng Feng Shengwu Ma Aoxue Wang Source Type: research
The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells
Transgenic Res. 2023 May 29. doi: 10.1007/s11248-023-00352-y. Online ahead of print.ABSTRACTJC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Hua-Chuan Zheng Hang Xue Hong-Zhi Sun Wen-Jing Yun Zheng-Guo Cui Source Type: research
A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants
In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, ...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Mst Sufara Akhter Banu Kazi Md Kamrul Huda Md Harun-Ur-Rashid Shahanaz Parveen Narendra Tuteja Source Type: research
