Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
Establishment of a hydrodynamic delivery system in ducks
In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein. The highest expression of SEAP occurred when ducks were injected with 5 µg/mL plasmid pLIVE-SEAP in 10% bodyweight volume of physiological saline for 6 s. To verify the distribution and expression of exogenous genes in multiple tissues, the relative level of foreign gene DNA and β-galactosidase staining of LacZ were evaluated, which showed the plasmids and their products were located mainly in the liver. Additionally, β-galactosidase staining and fluorescence imaging indicated the delivered exogenous g...
Source: Transgenic Research - March 9, 2024 Category: Genetics & Stem Cells Authors: Zhanji Zhao Jiabing Zhu Lijian Zhou Nan Sun Kaile Chang Xiaoyue Hu Yuting Hu Mingzhi Ren Yan Cheng Derong Xu Hongbo Xin Chunbo Zhang Source Type: research
