β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis < sup > a < /sup > structure on N-glycan in Oryza sativa
Transgenic Res. 2023 Aug 4. doi: 10.1007/s11248-023-00360-y. Online ahead of print.ABSTRACTβ1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate ge...
Source: Transgenic Research - August 4, 2023 Category: Genetics & Stem Cells Authors: Jae-Wan Jung Seong-Ryong Kim Source Type: research
β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis < sup > a < /sup > structure on N-glycan in Oryza sativa
Transgenic Res. 2023 Aug 4. doi: 10.1007/s11248-023-00360-y. Online ahead of print.ABSTRACTβ1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate ge...
Source: Transgenic Research - August 4, 2023 Category: Genetics & Stem Cells Authors: Jae-Wan Jung Seong-Ryong Kim Source Type: research
β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis < sup > a < /sup > structure on N-glycan in Oryza sativa
Transgenic Res. 2023 Aug 4. doi: 10.1007/s11248-023-00360-y. Online ahead of print.ABSTRACTβ1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate ge...
Source: Transgenic Research - August 4, 2023 Category: Genetics & Stem Cells Authors: Jae-Wan Jung Seong-Ryong Kim Source Type: research
β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis < sup > a < /sup > structure on N-glycan in Oryza sativa
Transgenic Res. 2023 Aug 4. doi: 10.1007/s11248-023-00360-y. Online ahead of print.ABSTRACTβ1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate ge...
Source: Transgenic Research - August 4, 2023 Category: Genetics & Stem Cells Authors: Jae-Wan Jung Seong-Ryong Kim Source Type: research
β1,3-galactosyltransferase on chromosome 6 is essential for the formation of Lewis < sup > a < /sup > structure on N-glycan in Oryza sativa
Transgenic Res. 2023 Aug 4. doi: 10.1007/s11248-023-00360-y. Online ahead of print.ABSTRACTβ1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available. I therefore characterized three GalT candidate ge...
Source: Transgenic Research - August 4, 2023 Category: Genetics & Stem Cells Authors: Jae-Wan Jung Seong-Ryong Kim Source Type: research
Co-expression of four penaeidins in transgenic rice seeds: an alternative strategy for substitute antibiotic agricultural products
Transgenic Res. 2023 Aug 3. doi: 10.1007/s11248-023-00361-x. Online ahead of print.ABSTRACTThe co-expression of multiple antimicrobial peptides (AMPs) in genetically modified (GM) crops can give plants a broader antibacterial spectrum and lower the pathogen risk of drug resistance. Therefore, four penaeidins (shrimp-derived AMPs) were fused and encoded in an artificial gene (PEN1234), driven by the seed-specific promoter Pzein, with the aim of co-expression in seeds of transgenic rice. The resistant rice plants, acquired via Agrobacterium-mediated transformation and glufosinate screening, were identified by PCR and the mod...
Source: Transgenic Research - August 3, 2023 Category: Genetics & Stem Cells Authors: Xinyuan Song Yu Qiao Jian Ma Xue Zhang Jie Liu Wen Xin Shaochen Xing Yunpeng Wang Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
