Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis
Transgenic Res. 2023 Sep 13. doi: 10.1007/s11248-023-00367-5. Online ahead of print.ABSTRACTCitrus is one of the major horticultural crops with high economic and nutraceutical value. Despite the fact that conventional research has developed numerous improved varieties, citriculture is still susceptible to various stresses and requires innovative solutions such as genetic engineering. Among all the currently available modern approaches, Agrobacterium-mediated transformation is the most efficient method for introducing desired traits in citrus. However, being a non-host for Agrobacterium, various citrus species, including Ci...
Source: Transgenic Research - September 13, 2023 Category: Genetics & Stem Cells Authors: Sweta Singh Zeba Tarannum Sunil Kokane Dilip K Ghosh Ashwani K Sharma Harsh Chauhan Source Type: research
Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis
Transgenic Res. 2023 Sep 13. doi: 10.1007/s11248-023-00367-5. Online ahead of print.ABSTRACTCitrus is one of the major horticultural crops with high economic and nutraceutical value. Despite the fact that conventional research has developed numerous improved varieties, citriculture is still susceptible to various stresses and requires innovative solutions such as genetic engineering. Among all the currently available modern approaches, Agrobacterium-mediated transformation is the most efficient method for introducing desired traits in citrus. However, being a non-host for Agrobacterium, various citrus species, including Ci...
Source: Transgenic Research - September 13, 2023 Category: Genetics & Stem Cells Authors: Sweta Singh Zeba Tarannum Sunil Kokane Dilip K Ghosh Ashwani K Sharma Harsh Chauhan Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants
In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgeni...
Source: Transgenic Research - September 1, 2023 Category: Genetics & Stem Cells Authors: Maryam Khoshnami Bahar Zare Hamideh Mardani-Mehrabad Farshad Rakhshandehroo Mohammad Amin Baghery Mohammad Ali Malboobi Source Type: research
Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster
This study represents the first step toward the establishment of n-3 PUFA-producing insects.PMID:37615877 | DOI:10.1007/s11248-023-00363-9 (Source: Transgenic Research)
Source: Transgenic Research - August 24, 2023 Category: Genetics & Stem Cells Authors: Mai Sato Ryoma Ota Satoru Kobayashi Kimiko Yamakawa-Kobayashi Takeshi Miura Atsushi Ido Yuya Ohhara Source Type: research
Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster
This study represents the first step toward the establishment of n-3 PUFA-producing insects.PMID:37615877 | DOI:10.1007/s11248-023-00363-9 (Source: Transgenic Research)
Source: Transgenic Research - August 24, 2023 Category: Genetics & Stem Cells Authors: Mai Sato Ryoma Ota Satoru Kobayashi Kimiko Yamakawa-Kobayashi Takeshi Miura Atsushi Ido Yuya Ohhara Source Type: research
Bioproduction of n-3 polyunsaturated fatty acids by nematode fatty acid desaturases and elongase in Drosophila melanogaster
This study represents the first step toward the establishment of n-3 PUFA-producing insects.PMID:37615877 | DOI:10.1007/s11248-023-00363-9 (Source: Transgenic Research)
Source: Transgenic Research - August 24, 2023 Category: Genetics & Stem Cells Authors: Mai Sato Ryoma Ota Satoru Kobayashi Kimiko Yamakawa-Kobayashi Takeshi Miura Atsushi Ido Yuya Ohhara Source Type: research
