The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells
Transgenic Res. 2023 May 29. doi: 10.1007/s11248-023-00352-y. Online ahead of print.ABSTRACTJC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Hua-Chuan Zheng Hang Xue Hong-Zhi Sun Wen-Jing Yun Zheng-Guo Cui Source Type: research
A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants
In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, ...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Mst Sufara Akhter Banu Kazi Md Kamrul Huda Md Harun-Ur-Rashid Shahanaz Parveen Narendra Tuteja Source Type: research
The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells
Transgenic Res. 2023 May 29. doi: 10.1007/s11248-023-00352-y. Online ahead of print.ABSTRACTJC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Hua-Chuan Zheng Hang Xue Hong-Zhi Sun Wen-Jing Yun Zheng-Guo Cui Source Type: research
A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants
In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, ...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Mst Sufara Akhter Banu Kazi Md Kamrul Huda Md Harun-Ur-Rashid Shahanaz Parveen Narendra Tuteja Source Type: research
The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells
Transgenic Res. 2023 May 29. doi: 10.1007/s11248-023-00352-y. Online ahead of print.ABSTRACTJC polyoma virus (JCPyV), a ubiquitous polyoma virus that commonly infects people, is identified as the etiologic factor for progressive multifocal leukoencephalopathy and has been closely linked to various human cancers. Transgenic mice of CAG-loxp-Laz-loxp T antigen were established. T-antigen expression was specifically activated in gastroenterological target cells with a LacZ deletion using a cre-loxp system. Gastric poorly-differentiated carcinoma was observed in T antigen-activated mice using K19-cre (stem-like cells) and PGC-...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Hua-Chuan Zheng Hang Xue Hong-Zhi Sun Wen-Jing Yun Zheng-Guo Cui Source Type: research
A DEAD box helicase Psp68 positively regulates salt stress responses in marker-free transgenic rice plants
In this study, salinity tolerant marker-free transgenic rice has been developed by overexpressing Psp68 gene and phenotypically characterized. The Psp68 overexpressing marker-free transgenic rice plants were initially screened in the rooting medium containing salt stress and 20% polyethylene glycol (PEG). Stable integration and overexpression of Psp68 in marker-free transgenic lines were confirmed by molecular analyses including PCR, southern, western blot, and qRT-PCR analyses. The marker-free transgenic lines showed enhanced tolerance to salinity stress as displayed by early seed germination, higher chlorophyll content, ...
Source: Transgenic Research - May 29, 2023 Category: Genetics & Stem Cells Authors: Mst Sufara Akhter Banu Kazi Md Kamrul Huda Md Harun-Ur-Rashid Shahanaz Parveen Narendra Tuteja Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
Comparative safety assessment of genetically modified crops: focus on equivalence with reference varieties could contribute to more efficient and effective field trials
Transgenic Res. 2023 May 22. doi: 10.1007/s11248-023-00344-y. Online ahead of print.ABSTRACTThe initial compositional analysis of plants plays an important role within the internationally harmonized comparative safety assessment approach for genetically modified plants. Current EFSA guidance prescribes two types of comparison, namely difference tests with regard to a conventional comparator or control, and equivalence tests with regard to a collection of commercial reference varieties. The experience gained so far shows that most of the statistically significant differences between the test and control can be discounted ba...
Source: Transgenic Research - May 22, 2023 Category: Genetics & Stem Cells Authors: Gijs A Kleter Hilko van der Voet Jasper Engel Jan-Pieter van der Berg Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies
This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.PMID:37166587 | DOI:10.1007/s11248-023-00347-9 (Source: Transgenic Research)
Source: Transgenic Research - May 11, 2023 Category: Genetics & Stem Cells Authors: Margit Drapal Eugenia M A Enfissi Juliana Almeida Elzbieta Rapacz Marilise Nogueira Paul D Fraser Source Type: research
