Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
This study used three target regions (T1, T2, and T3) in gbss exon I, whose sequences were first inserted into the BbsI sites in the appropriate guide RNA (gRNA) vector (pEn-Chimera, pMR203, pMR204, and pMR205), and then localized between the AtU6 promoter and the gRNA scaffold sequence. Expression vectors were constructed by introducing gRNA genes into the pMR287 (pYUCas9Plus) plasmids using the MultiSite Gateway system by attR and attL sites. The three target regions of mutant potato lines were analyzed. The use of CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis allowed tri- or tetra-allelic mutant potato li...
Source: Transgenic Research - June 18, 2023 Category: Genetics & Stem Cells Authors: Laura Abeuova Balnur Kali Dilnur Tussipkan Ainash Akhmetollayeva Yerlan Ramankulov Shuga Manabayeva Source Type: research
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
This study used three target regions (T1, T2, and T3) in gbss exon I, whose sequences were first inserted into the BbsI sites in the appropriate guide RNA (gRNA) vector (pEn-Chimera, pMR203, pMR204, and pMR205), and then localized between the AtU6 promoter and the gRNA scaffold sequence. Expression vectors were constructed by introducing gRNA genes into the pMR287 (pYUCas9Plus) plasmids using the MultiSite Gateway system by attR and attL sites. The three target regions of mutant potato lines were analyzed. The use of CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis allowed tri- or tetra-allelic mutant potato li...
Source: Transgenic Research - June 18, 2023 Category: Genetics & Stem Cells Authors: Laura Abeuova Balnur Kali Dilnur Tussipkan Ainash Akhmetollayeva Yerlan Ramankulov Shuga Manabayeva Source Type: research
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
This study used three target regions (T1, T2, and T3) in gbss exon I, whose sequences were first inserted into the BbsI sites in the appropriate guide RNA (gRNA) vector (pEn-Chimera, pMR203, pMR204, and pMR205), and then localized between the AtU6 promoter and the gRNA scaffold sequence. Expression vectors were constructed by introducing gRNA genes into the pMR287 (pYUCas9Plus) plasmids using the MultiSite Gateway system by attR and attL sites. The three target regions of mutant potato lines were analyzed. The use of CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis allowed tri- or tetra-allelic mutant potato li...
Source: Transgenic Research - June 18, 2023 Category: Genetics & Stem Cells Authors: Laura Abeuova Balnur Kali Dilnur Tussipkan Ainash Akhmetollayeva Yerlan Ramankulov Shuga Manabayeva Source Type: research
CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis in the potato
This study used three target regions (T1, T2, and T3) in gbss exon I, whose sequences were first inserted into the BbsI sites in the appropriate guide RNA (gRNA) vector (pEn-Chimera, pMR203, pMR204, and pMR205), and then localized between the AtU6 promoter and the gRNA scaffold sequence. Expression vectors were constructed by introducing gRNA genes into the pMR287 (pYUCas9Plus) plasmids using the MultiSite Gateway system by attR and attL sites. The three target regions of mutant potato lines were analyzed. The use of CRISPR/Cas9-mediated multiple guide RNA-targeted mutagenesis allowed tri- or tetra-allelic mutant potato li...
Source: Transgenic Research - June 18, 2023 Category: Genetics & Stem Cells Authors: Laura Abeuova Balnur Kali Dilnur Tussipkan Ainash Akhmetollayeva Yerlan Ramankulov Shuga Manabayeva Source Type: research
Large scale sampling of Mexican maize landraces for the presence of transgenes
Transgenic Res. 2023 Jun 16. doi: 10.1007/s11248-023-00357-7. Online ahead of print.ABSTRACTThe presence and levels of transgenic maize in Mexico and the effect this could have on local landraces or closely related species such as teosinte has been the subject of several previous reports, some showing contrasting results. Cultural, social and political factors all affect maize cultivation in Mexico and although since 1998 there has been a moratorium on the commercial cultivation of transgenic maize, Mexico imports maize, mainly from the USA where transgenic cultivars are widely grown. Additionally extensive migration betwe...
Source: Transgenic Research - June 16, 2023 Category: Genetics & Stem Cells Authors: E Adriana Ceniceros-Ojeda Corina Hayano-Kanashiro Octavio Mart ínez M Humberto Reyes-Vald és Fernando Hern ández-Godinez Jos é Luis Pons-Hernández June Simpson Source Type: research
Large scale sampling of Mexican maize landraces for the presence of transgenes
Transgenic Res. 2023 Jun 16. doi: 10.1007/s11248-023-00357-7. Online ahead of print.ABSTRACTThe presence and levels of transgenic maize in Mexico and the effect this could have on local landraces or closely related species such as teosinte has been the subject of several previous reports, some showing contrasting results. Cultural, social and political factors all affect maize cultivation in Mexico and although since 1998 there has been a moratorium on the commercial cultivation of transgenic maize, Mexico imports maize, mainly from the USA where transgenic cultivars are widely grown. Additionally extensive migration betwe...
Source: Transgenic Research - June 16, 2023 Category: Genetics & Stem Cells Authors: E Adriana Ceniceros-Ojeda Corina Hayano-Kanashiro Octavio Mart ínez M Humberto Reyes-Vald és Fernando Hern ández-Godinez Jos é Luis Pons-Hernández June Simpson Source Type: research
Large scale sampling of Mexican maize landraces for the presence of transgenes
Transgenic Res. 2023 Jun 16. doi: 10.1007/s11248-023-00357-7. Online ahead of print.ABSTRACTThe presence and levels of transgenic maize in Mexico and the effect this could have on local landraces or closely related species such as teosinte has been the subject of several previous reports, some showing contrasting results. Cultural, social and political factors all affect maize cultivation in Mexico and although since 1998 there has been a moratorium on the commercial cultivation of transgenic maize, Mexico imports maize, mainly from the USA where transgenic cultivars are widely grown. Additionally extensive migration betwe...
Source: Transgenic Research - June 16, 2023 Category: Genetics & Stem Cells Authors: E Adriana Ceniceros-Ojeda Corina Hayano-Kanashiro Octavio Mart ínez M Humberto Reyes-Vald és Fernando Hern ández-Godinez Jos é Luis Pons-Hernández June Simpson Source Type: research
