CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
CRISPR/Cas9-mediated knock-in of masu salmon (Oncorhyncus masou) elongase gene in the melanocortin-4 (mc4r) coding region of channel catfish (Ictalurus punctatus) genome
Transgenic Res. 2023 Jul 19. doi: 10.1007/s11248-023-00346-w. Online ahead of print.ABSTRACTChannel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%. Fish containing bo...
Source: Transgenic Research - July 19, 2023 Category: Genetics & Stem Cells Authors: Michael Coogan De Xing Baofeng Su Veronica Alston Andrew Johnson Mohd Khan Karim Khalil Ahmed Elaswad Shangjia Li Jinhai Wang Cuiyu Lu Wenwen Wang Darshika Hettiarachchi Mei Shang Tasnuba Hasin Zhenkui Qin Roger Cone Ian A E Butts Rex A Dunham Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Protein familiarity is a fundamental but rarely operationalized concept in the safety assessment of genetically modified crops: example of phosphomannose isomerase (PMI)
Transgenic Res. 2023 Jul 6. doi: 10.1007/s11248-023-00358-6. Online ahead of print.ABSTRACTFundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by r...
Source: Transgenic Research - July 6, 2023 Category: Genetics & Stem Cells Authors: Anne B Carlson Carey A Mathesius Tim A Gunderson Aideen Hession Reba Bruyere Henry P Mirsky John Zhang Mat Sandmann Melissa N Fallers Rod A Herman Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
Evaluation of cucumber UBL5 promoter as a tool for transgene expression and genome editing in plants
This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined. The results showed that the CsUBL5 promoter effectively drove transgene expressio...
Source: Transgenic Research - June 23, 2023 Category: Genetics & Stem Cells Authors: Kamonchanok Taway Issariya Dachphun Supachai Vuttipongchaikij Anongpat Suttangkakul Source Type: research
